Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,261,811	0	T	G	40.6%	84.7 / 28.3	66	K185N (AAA→AAC)	fruK	fructose‑1‑phosphate kinase
Reads supporting (aligned to +/- strand):  ref base T (11/27);  new base G (26/0);  total (39/27)
Fisher's exact test for biased strand distribution p-value = 1.65e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACA  >  NC_000913/2261661‑2261960                                                                                                                                                        |                                                                                                                                                         ggAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGt                                                                                                                                                          <  2:460972/151‑1 (MQ=255)       aTTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGGTTCAcc                                                                                                                                                    >  1:631527/1‑151 (MQ=255)         tAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGGTTCAcc                                                                                                                                                    >  2:121766/1‑149 (MQ=255)          aaCCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTTGGTTTTTCCAGc                                                                                                                                                 >  2:1159920/1‑151 (MQ=255)            cccAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCTCATCTTTCATTTCAGGCAGTTTGCGGCCTGCCTTGATTTCCAGCTCGCGGCGG‑TTAGGTTTCTCCAGcc                                                                                                                                                <  2:215392/150‑1 (MQ=255)            cccAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGGTTTCCCAGcc                                                                                                                                                >  2:152005/1‑150 (MQ=255)                aaGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGGTTTACCAGCCACggg                                                                                                                                           >  1:571666/1‑150 (MQ=255)                       ccTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑tt                                                                                                                                  <  1:870629/151‑1 (MQ=255)                            ggCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCaaa                                                                                                                             <  2:29080/151‑1 (MQ=255)                            ggCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGGTTTACCAGCCCCGGGGCCG‑C‑CTTCaa                                                                                                                              >  2:873055/1‑150 (MQ=255)                             gCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGGTTTAACAGGCCCCGGGCCG‑C‑TTTCaaa                                                                                                                             >  2:62085/1‑150 (MQ=255)                              cACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAAcc                                                                                                                           <  2:352363/151‑1 (MQ=255)                                 ccAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGc                                                                                                                        <  2:182902/151‑1 (MQ=255)                                  cAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGct                                                                                                                       <  1:895890/151‑1 (MQ=255)                                   aGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGGTTCACCAGCCACGGGGCCG‑C‑TTTCAAAACTGc                                                                                                                        >  1:651598/1‑149 (MQ=255)                                     tGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGGGCCG‑C‑TTTCAAACCTGctac                                                                                                                     >  1:1007063/1‑150 (MQ=255)                                      gAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGctact                                                                                                                    <  1:1158390/150‑1 (MQ=255)                                                caTGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTTGGGTTTACCCACCCCCGTGCCC‑C‑TTTTAAACCCCCTCCTAA‑CGCTTTAc                                                                                                         >  2:551127/1‑150 (MQ=255)                                                 aTGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACgg                                                                                                       <  2:1035396/151‑1 (MQ=255)                                                   gcgcGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGc                                                                                                      <  2:268781/150‑1 (MQ=255)                                                    cgcgATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTTACCAGCCCCGGGGCCG‑C‑CTTTAAACCTGCTACTAA‑CGCTTCACgg                                                                                                       >  2:159630/1‑148 (MQ=255)                                                            cTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGGTTCACCAGCCACGGGGCCG‑C‑CTTCAAAACTGCCACTAA‑AGCTTCACCGCTACTATTaa                                                                                             >  1:128482/1‑150 (MQ=255)                                                               gTTCACGTAGCGCATGTGCAGCTTCAATCGCATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCTAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTAATAA‑CGCTTCACGGCTACTATCa                                                                                              <  2:110179/146‑1 (MQ=255)                                                                 tCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAACCCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGa                                                                                          <  1:503469/148‑1 (MQ=255)                                                                   aCGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTTGGTTTTACCCGCCCCCGTGCCG‑CTTTTCAAACC‑GGTCCCTA‑CGCCTCTCGGCTACCATCaaa                                                                                            >  2:106685/1‑144 (MQ=255)                                                                   aCGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGGTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGaaaa                                                                                       >  1:923856/1‑149 (MQ=255)                                                                     gTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGACTACTATCAAAGATAATGCa                                                                                   <  1:698113/151‑1 (MQ=255)                                                                       aGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTTAGGTTCACCAGCCACGGGGCCC‑C‑CTTCAAAACTGCTACCAA‑CGCTTCACCGCAACTATCAAAGATAATg                                                                                     >  1:919772/1‑147 (MQ=255)                                                                          gcATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGac                                                                              <  2:594447/151‑1 (MQ=255)                                                                              gtgCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACt                                                                           <  1:515228/150‑1 (MQ=255)                                                                                 cAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTACAGCTCGCGGCGG‑GTAGGGTTTACCAGCCACAGCGGCG‑G‑CTTCTAACCTGCTACTAA‑AACCTCTCCGATCCTATCAAAGAAAATGCAAAGACACtatct                                                                       >  2:506436/1‑147 (MQ=255)                                                                                  aGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTa                                                                      <  2:624530/151‑1 (MQ=255)                                                                                  aGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGGTTCACCCGCCCCCGGGCCC‑C‑TTTTAAAACCGCTACTAA‑CGCTTCACCGCTACTATCAAAGAAAAAGCa                                                                                   >  2:988563/1‑138 (MQ=255)                                                                                    cTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTTGGGTTTACCAGCCACGGTGCCG‑C‑CTTTAAACCTGCTACTAA‑AGCCTCACGGCTACTATCAAAAATAAAGCAAGGGCAATGACTAc                                                                     >  2:795243/1‑150 (MQ=255)                                                                                     ttCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTTCGTTTCAACAACCAAAGTGCCG‑C‑TTTCAAACCTTCTTCTAA‑CGCTTCACGGCTACTATCAAAGATAATGCCAGGACACTGACTaa                                                                     >  1:1048391/1‑148 (MQ=255)                                                                                      tCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACg                                                                    <  1:467362/149‑1 (MQ=255)                                                                                      tCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGGTTCACCAACCACCGGGCCG‑C‑CTTTAAAACCGCTAATTA‑AGCTTCACGGCCACTATCAAAGATAAAGCAAAGACACTGACCAcac                                                                   >  2:803387/1‑148 (MQ=255)                                                                                       cAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTTGGGTTTACCAGCCACGGTGCCG‑C‑CTTCAAACCCGCTTCTAA‑CCCTTCCCAGGTACTATTaaa                                                                                            >  2:156436/1‑124 (MQ=255)                                                                                        aaTCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGGTTTACCACCCACCCTGCCG‑C‑TTTCAAAACCGCTGCTCA‑CTCCTCCCCGCTACTATCCAAAATaaatcaaag                                                                                >  2:875351/1‑128 (MQ=255)                                                                                         aTCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGGTTTACCCGCCACGGGGCCG‑C‑TTTCAAACCATCTAATAA‑AGCCTCACGGCCACCATCAAAAATAATGAAAGGACACTGACTACGCAgg                                                                >  2:385665/1‑150 (MQ=255)                                                                                          tCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTTGGGTTTACCCGCCACGGGGCCG‑C‑CTTCAAAACCGCTACTAA‑CGCTTCACGGCTACTATCCAAGATAATGCAAGGACACCGACTAAGCAgg                                                                >  2:198375/1‑149 (MQ=255)                                                                                           cacaTCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCg                                                              <  1:726485/150‑1 (MQ=255)                                                                                           cacaTCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGa                                                             <  1:801014/151‑1 (MQ=255)                                                                                             caTCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTTACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGt                                                           >  1:436623/1‑151 (MQ=255)                                                                                               tCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTTGGGTTTACCACCCCCGGGGCCG‑C‑CTTCAAACCTGCTACTAA‑CCCTTCCCCGCTACTATCCAAGATAATGCAAAGACACTGAATACGCAAGCggggc                                                          >  2:1005449/1‑146 (MQ=255)                                                                                                cTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGGTTTACCAGCCCCCGGGCCC‑C‑TTTTAAACCCGCTACCAA‑CGCTTCACCGCCACTATCAAAAAAAATGCCAGGACACCTAATACGCAAGCGAggc                                                          >  2:616422/1‑147 (MQ=255)                                                                                                 tttcatttcaGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGGTTTCCCAGCCCCGGTGGCG‑C‑TTTCAAACCCTCTACTAA‑CGCTTCACGGCTAATATCCAAGATAATGCAAAGACACCGACTTAGCAGGCGAGGCAt                                                        >  1:418961/1‑150 (MQ=255)                                                                                                  ttcatttcaGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTc                                                          <  1:416203/147‑1 (MQ=255)                                                                                                  ttcatttcaGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCa                                                         <  2:256989/148‑1 (MQ=255)                                                                                                  ttcatttcaGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCAt                                                        <  1:644018/149‑1 (MQ=255)                                                                                                  ttcatttcaGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGGTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATcc                                                      >  1:776580/1‑151 (MQ=255)                                                                                                    catttcaGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTATGCTTTCCCCGCCACCGTGCCC‑C‑TTTTCAAACTCCTCCTAAGCCCTTC‑CAGCCACTATTCAAAATAATGCAAAGACACATACTACGCAGGCGAGGCATcc                                                      >  2:1171007/1‑149 (MQ=255)                                                                                                        tcaGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCg                                                 <  1:692079/150‑1 (MQ=255)                                                                                                         caGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCTCGGCGGATGAGGTTT‑ACCAGACCCAGAGTCG‑C‑GTTCAAACCCACTCCTTA‑CGCTTCGCAGCTCCTGTCGAAGGTGATGAGAGGGCACTGACTacgcacgccaatctgcccgacg                                                 >  2:253113/1‑135 (MQ=255)                                                                                                         caGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGGTTCACCAGCCACGGGGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCgg                                                >  1:896971/1‑150 (MQ=255)                                                                                                            gCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGaa                                            <  2:487792/151‑1 (MQ=255)                                                                                                              cgTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTTTGGTTTACCAGCCCCGGGGCCG‑C‑TTTTCAACCTTCTACTAA‑CGCTTTCCGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCCGTCGGTGaa                                            >  2:967073/2‑149 (MQ=255)                                                                                                                   aCGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGGCATCCAGTCGGGGAACGCTTcc                                     >  2:40723/1‑151 (MQ=255)                                                                                                                          gCCCAGATTTCCAGCTCGCGGCGGTTTAGGTTT‑ACCAACCCCGGGGCCGCC‑TTTCAAACC‑GCTACTTA‑AGCTTTACGGCCACCTTCAAAAATAATGGAAGGAAACCGACCACCCAGGCGAGTCATCCAAGCGGTTAAAGCCTcc                                     >  2:477512/1‑144 (MQ=255)                                                                                                                              aGATTTCCAGCTCGCGGCGG‑TTTGGGTTCACCAACCACGGTGCCG‑G‑TTTCAAACCCGCTCCCAA‑CGGTTCCCGGCCCCCTACAAAGATAAAGGAAGGACACTACCTAACCCGGGGGGTCCTCCACCAGGTGAACCAGTCCCGGGTGACGcc                          >  2:713347/1‑151 (MQ=255)                                                                                                                                  tgccAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGa                       <  1:447489/148‑1 (MQ=255)                                                                                                                                    ccAGCTCGCGGCGG‑TTATGATTTACCCGCCACCGTGCCG‑C‑TTTCCAACCCGCTACTAA‑AGCTTCACCGCTACTATCAAAGATAATGCAAGGACACTGTCTACGCAGGCGAGTCATTCAGTCGGGGAACGCGTTCGGGCTGGCGCCTGATgg                    >  2:401267/1‑151 (MQ=255)                                                                                                                                          cgcgGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAg                <  1:174718/149‑1 (MQ=255)                                                                                                                                             ggcgg‑TTAGGTTTCAACAGAGACGGGGGCG‑C‑ATTCAAAACTGCTACTAA‑GGCTTCAAGGGTACTATCAAAGATAATGCAAGGACAATGACTACGCAGGCGAGTCATCCAGTCGGGGAACGATTCCGGGCAGACGCCTGACGGGAAGCTTcc           >  2:858241/1‑151 (MQ=255)                                                                                                                                                gg‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCt        >  1:94829/1‑151 (MQ=255)                                                                                                                                                     aGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGac     <  1:1040681/150‑1 (MQ=255)                                                                                                                                                       gTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGAcaca  <  2:507306/151‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                         GGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGCGATGCCTTGTTCACGTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGG‑TTAGGTTTCACCAGCCACGGTGCCG‑C‑TTTCAAACCTGCTACTAA‑CGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACA  >  NC_000913/2261661‑2261960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑