Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,434,882	0	T	G	41.6%	124.7 / 23.8	79	Y252S (TAC→TCC)	truA	tRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base T (9/36);  new base G (32/0);  total (43/36)
Fisher's exact test for biased strand distribution p-value = 7.93e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCT  >  NC_000913/2434733‑2435024                                                                                                                                                       |                                                                                                                                                 cAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGt                                                                                                                                                  <  1:62815/150‑1 (MQ=255) cAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGt                                                                                                                                                  <  2:149881/150‑1 (MQ=255)   ggTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGTAGATAAT‑ACCCGGCAGGGGaa                                                                                                                                                >  2:259672/1‑150 (MQ=255)     tCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCAT‑ACCGGGCAGGGGaa                                                                                                                                                >  1:526630/1‑148 (MQ=255)      ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAATAATAT‑ATCAGTCAAGGTaa                                                                                                                                                >  1:54420/1‑147 (MQ=255)      ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATcc                                                                                                                                             <  2:636866/150‑1 (MQ=255)          atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAACAT‑ACCGGTTAGGGGCAACCACcgc                                                                                                                                        >  1:902380/1‑151 (MQ=255)            ttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATTAT‑ACCGGTCAGGGGAATCCACcgc                                                                                                                                        >  2:447957/1‑149 (MQ=255)             tGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATAAT‑ACCGGGCAGGGGAATCCACCGCCAc                                                                                                                                     >  1:867336/1‑151 (MQ=255)               ccTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCa                                                                                                                                   <  2:882950/151‑1 (MQ=255)               ccTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCa                                                                                                                                   <  2:335836/151‑1 (MQ=255)                  aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCAT‑ACCGGTCAGGGGAATCCACCCCGAcc                                                                                                                                    >  1:888354/1‑147 (MQ=255)                  aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGAt                                                                                                                                <  2:430063/151‑1 (MQ=255)                    gCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCAT‑AACGGGCAGGGGAAACCACCCCGACCAGATa                                                                                                                               >  1:214535/1‑150 (MQ=255)                    gCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCAT‑AACGGGCCGGGGAAACCACCCCGAcc                                                                                                                                    >  2:218510/1‑145 (MQ=255)                     ccTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACa                                                                                                                             <  2:659496/151‑1 (MQ=255)                       ttGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGATTTGGGAGAACATAACGGGTCAGGG‑AAAACACCCCGAcc                                                                                                                                    >  1:810867/1‑142 (MQ=255)                          tACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAACAT‑ACCCGGCCGGGGAATACACCCCGACCAGATACAA‑ccc                                                                                                                        >  2:1065315/1‑150 (MQ=255)                           aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCtt                                                                                                                      <  2:55871/151‑1 (MQ=255)                              cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTc                                                                                                                     <  1:1153791/149‑1 (MQ=255)                              cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCg                                                                                                                   <  2:578364/151‑1 (MQ=255)                               gTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGc                                                                                                                  <  2:308378/151‑1 (MQ=255)                                 cATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAACAT‑AACCGGCCGGGGAATCCACCCCGGCCAGAAAACG‑CCCCTccccct                                                                                                                >  2:877970/1‑147 (MQ=255)                                 cATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCGT‑AAACGGCAGAGAAATCCCCCACCAACAGATACAG‑cccctcccct                                                                                                                 >  2:560493/1‑150 (MQ=255)                                       tCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAAAACCT‑AACGGCCCGGGGAAACTACCGCGGCCAGATAAAA‑CCCTTCCGCttttt                                                                                                             >  1:408710/1‑147 (MQ=255)                                        cAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCAT‑ACCGGTCAGGGGAATCCACCCCGACCAGATACAG‑CCCTTCCCCTTTTTcc                                                                                                           >  1:127923/1‑149 (MQ=255)                                           gCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCAT‑ACCGGGCAGGGGAATACCCCCCGACCCGAAAAAA‑CCCCTCCCCCTTTTCCGtt                                                                                                        >  2:484323/1‑149 (MQ=255)                                               tAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAACAT‑ACCGGGTAGGGGAAACCACCCCCACCAGCTACAA‑CCCTTCCGCCTTTTCCGTtgctg                                                                                                    >  1:370242/1‑149 (MQ=255)                                               tAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGTAGAACAT‑ATCGGTCACGGGAATCCCCCACCGCCCGCTAAAG‑CCCCTCCGCCTTTTCCgct                                                                                                        >  2:773135/1‑143 (MQ=255)                                                aaCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGTAGATCAT‑ACCCGTCAGGGGAATTCACCGCGGCCAGAAACAA‑CCCTTTCGCCTTTTCCGGTGCTgcc                                                                                                  >  2:794033/1‑150 (MQ=255)                                                aaCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTgcc                                                                                                  <  2:1093880/150‑1 (MQ=255)                                                aaCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTgcc                                                                                                  <  1:861772/150‑1 (MQ=255)                                                  cTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCCGGCCGGGGAATCCACCCCGACCCGAAAAAG‑CCCCTCCGCTTTTGCCGTTTCTgccccc                                                                                               >  1:970316/1‑151 (MQ=255)                                                    aCGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCa                                                                                              <  1:282719/150‑1 (MQ=255)                                                     cGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCa                                                                                              <  2:484543/149‑1 (MQ=255)                                                       ttGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCAT‑ACCGGTCAGGGGAATTCACCCCCACCCGAAACAG‑CCCCTCCCCCTTTTCCGTTGCTGccgcccgc                                                                                            >  2:1098618/1‑149 (MQ=255)                                                         ggCTACAGCCTGAAATGTACCGAGCATTATTCTCGTTAGTCCGCCAGAAAGAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCTCCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGtt                                                                                         <  1:1065837/150‑1 (MQ=255)                                                               aGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCt                                                                                 <  1:515170/151‑1 (MQ=255)                                                                  cTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCAT‑ACCGGTCAGGGGAATCCACCGCGGCCCGAAAAAA‑CCCCTCCGCTTTTTTCGTTGCTGCCCCCAGCGGTC‑TTTCCtttt                                                                              >  1:109069/1‑150 (MQ=255)                                                                       aTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGAACAT‑AACCGTCAGGGGGAATCACCCCGACCAGAAAAAA‑ACCTTTCGCTTTTTCCGTTGCTTGCGCCCGCGTTC‑TGTCCcttttcccc                                                                         >  1:431155/1‑145 (MQ=255)                                                                        tGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAGCAT‑ACCCGGCAGGGGAATCCACCCCGACCCGCTACAA‑CCCCTCCCCTTTTGTCCTTGCTTACCCCCGGGTTC‑TTTCCTTTtcccccc                                                                        >  2:59936/1‑150 (MQ=255)                                                                         gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCa                                                                        <  1:93070/150‑1 (MQ=255)                                                                          ttCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCAT‑ACCCGTCCGGGGAATCCACCCCGACCAGAAACAA‑CCCCTCCCCTTTTTTCGTTTCTGCCGCCAGCGTTC‑TGTCCTTTtccccc                                                                         >  2:434605/1‑148 (MQ=255)                                                                           tCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCAT‑ACCCGTCAGGGGAAACCACCCCCCCCAGATACAA‑CCCCTCCCCTTTTTCCGGTGCTGCCGCCCGCCGTC‑TTTCCTTTTccgcccgc                                                                      >  2:276149/1‑150 (MQ=255)                                                                                gCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑AACGGGCAGGGGAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGGTGCTGCCCCCAGCGTTC‑TGTCCCTTTCCGCCAGCAActc                                                                 >  1:300533/1‑150 (MQ=255)                                                                                 cATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTg                                                               <  1:1133215/151‑1 (MQ=255)                                                                                    tattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAACAT‑ACCCGGGAGGGGAATTCACCCCGGACAGATAAAG‑CCCTTTCCCTTTTGCCGTTTGTGCCGCCCGCGGTC‑TGTCCTTTTCCGCCACCAACTCTGc                                                              >  2:777437/1‑149 (MQ=255)                                                                                    tattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATTAT‑ACCGGTCAGGGGGATCCAACCCCCACAGATACAC‑CCCCTCCTCCTTTTCCGTTGCTTCCGCCCGCCGTC‑TGTTCTTTTTCGCCCGCaa                                                                    >  2:192961/1‑143 (MQ=255)                                                                                     attCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTa                                                            <  1:1172725/150‑1 (MQ=255)                                                                                     attCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTa                                                            <  2:743861/150‑1 (MQ=255)                                                                                      ttCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCAT‑ACCGGGCAGGGGAAACCACCCCGACCAGATACAG‑CCCTTCCGCTTTTTCCGGTGCTGCCGCCCGCGTTC‑TGTCCTTTTCCGCCCGCCACTCTGCTAt                                                           >  2:345409/1‑150 (MQ=255)                                                                                       tctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑AACGGGCAGGGGAAACCACCCCGACCAGAAAAAG‑CCCTTCCGCCTTTTCCGTTGCTGCCCCCAGCGGTC‑TGTCCTTTTCCGCCCGCAACTCTGCTATc                                                          >  1:556192/1‑150 (MQ=255)                                                                                          cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAATAT‑ACCCGGCAGGGTAATCCACCCCGACCCGATACAGACCCTTCCCCTTTT‑CCGTTGCTGCCGCCCGCGTTC‑TGTCCTTTTCCCCCCGCAAATCTTCTATCCAGc                                                      >  2:138096/1‑151 (MQ=255)                                                                                           gTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGGTTAT‑ACCGGGCTGGGTGAATCTCCACCACCAGGTACAA‑CCCTTCCGCTTTTTCCGTTGGTGCCGCCACCGCTC‑TTGCCTTTTCCGCCGGCAAATCTTCTaatca                                                        >  1:1119036/1‑144 (MQ=255)                                                                                             tAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATTAT‑ACCGGGCAAGGGAATCCACCCCCACCAGATACAA‑CCCCTCCGCTTTTTCCCGTGCTGCCCCCCGCGTTC‑TTTCCTTTTCCCCCCGCCAACCTGCTATCCaac                                                      >  2:181953/1‑146 (MQ=255)                                                                                                      aGAAATAGCGGGCCCATTGGCGGTTTTTGGAAATCAA‑AACCGGCAGGGGAATTCACCGCGACCAGATACAA‑CCCTTCCCCCTTTTCCGGTTGTGCCGCCAGCGTTC‑ATTCCTTTTCCGCCAGCGAATCCGGTATACATCTCTcc                                                 >  1:422743/1‑144 (MQ=255)                                                                                                      aGAAATAGCGGGCCCATTGGCGGTTTTGTGAAATCAT‑ACCCGGGAAGGGAATCCACCGCGACCAGAAAAAGACCCTTCC‑CTTTTGCCCCTTCTGCCGCCCCCGGTCTTGCCCTTTTCC‑CCCGCCACCCTGCTTTCCCGCTCTCCGGGTgg                                           >  2:27204/1‑150 (MQ=255)                                                                                                                ggCCCATTGGCGGGTTTTGAAGATCAT‑ACCGGTCAGGGGAATCCACCGCGACCAGATACAG‑CCCCTTCGCTTTTTCCGTTGCTCCCCCCCGCGGTC‑TGTCCCTTGCCCCCAGCCAACCTGCTATCCAACCCCCCGGCTGGGTTTGGGCAcc                                >  2:367182/1‑151 (MQ=255)                                                                                                                   ccATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAc                             <  2:431139/151‑1 (MQ=255)                                                                                                                    cATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACt                            <  2:203312/151‑1 (MQ=255)                                                                                                                         gCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCAt                       <  2:992499/151‑1 (MQ=255)                                                                                                                         gCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCAt                       <  1:947731/151‑1 (MQ=255)                                                                                                                          cGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc                      <  2:370229/151‑1 (MQ=255)                                                                                                                           ggTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCa                     <  1:479803/151‑1 (MQ=255)                                                                                                                            gTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAg                    <  2:526612/151‑1 (MQ=255)                                                                                                                            gTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCAGCTGGTTGTGGGCACCGACTTCCATc                      >  1:545781/1‑149 (MQ=255)                                                                                                                             ttttGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAgg                   >  1:588408/1‑151 (MQ=255)                                                                                                                               ttGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGc                  <  1:536313/150‑1 (MQ=255)                                                                                                                                tGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCt                 <  1:461451/150‑1 (MQ=255)                                                                                                                                tGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTg                >  1:636664/1‑151 (MQ=255)                                                                                                                                 ggAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTg                <  1:1113848/150‑1 (MQ=255)                                                                                                                                 ggAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGc               >  2:230983/1‑151 (MQ=255)                                                                                                                                 ggAAGATCAT‑ACCGGTCAGGGTAATCCACAGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGc               >  2:381581/1‑151 (MQ=255)                                                                                                                                      aTCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGAc           >  1:598142/1‑150 (MQ=255)                                                                                                                                         aT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAata       <  1:1034775/151‑1 (MQ=255)                                                                                                                                         aT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAata       <  1:766222/151‑1 (MQ=255)                                                                                                                                            aCCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAatat      <  1:162835/150‑1 (MQ=255)                                                                                                                                             ccGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTc    <  2:7442/151‑1 (MQ=255)                                                                                                                                              cGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTcc   <  1:649202/151‑1 (MQ=255)                                                                                                                                               ggTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCt  <  2:203515/151‑1 (MQ=255)                                                                                                                                               ggTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCt  <  2:3487/151‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                 CAGGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCAT‑ACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTC‑TGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCT  >  NC_000913/2434733‑2435024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑