| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,678,039 | 0 | T | G | 42.9% | 86.3 / 16.5 | 56 | N108T (AAC→ACC) | yphE | putative sugar ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base T (7/25); new base G (24/0); total (31/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.52e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGC > NC_000913/2677890‑2678186 | ccaGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGGGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGt < 1:72868/150‑1 (MQ=255) aGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTGACC‑GAGGGAGAGGgttt > 1:1001055/1‑151 (MQ=255) gctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCg < 2:1117451/150‑1 (MQ=255) gCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCt < 1:808016/151‑1 (MQ=255) gCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGGTTTTCGCCCCTGTCAGccc > 1:714149/1‑150 (MQ=255) gggTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCtt < 2:672853/145‑1 (MQ=255) gCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCCGACC‑GAGGCGGAGGGTTTTCGCCCCCGTCAGCCCCTcc > 2:879046/1‑149 (MQ=255) gTTGAAACAAGTTGTTCAGGACTAACGTCACCGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTccacca < 2:43379/151‑1 (MQ=255) gTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCAGTCCGGAGGC‑GAGGGTTTCTCGCACTGTCAGCCCCTccacc > 2:275313/1‑150 (MQ=255) gAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCCACC‑GAGGCAGAGGGTTTCCGCCCCCGTCAACCCTTccacc > 2:1047492/1‑147 (MQ=255) aaaCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACC‑CAGGCGGAGGGTTTTCCCCACTGCCCGCCCTTccccc > 2:769190/1‑146 (MQ=255) aaCAAGTTGTTCAGGACTAACGTCAACGCCCAGCCCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACtt < 1:187634/151‑1 (MQ=255) aGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACC‑GAGGGGGAGGGTTTTCGCCCCCTTCAGCCCTTCCCCCAGAcctta > 2:524650/1‑145 (MQ=255) gttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTg < 1:399925/151‑1 (MQ=255) cAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGAt < 2:1032634/150‑1 (MQ=255) aGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑CAGGGAGAGGGTTTCCCCCCCTGTCAGCCCTTCCACCAGACCTAAATCCCGATa > 1:9719/1‑150 (MQ=255) aCTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACC‑GCGGCAGAGGGTTTTCGCCCCTGTCAGCCCCTCCCCCAGACCTAACACCTGTTaaaac > 2:418016/1‑149 (MQ=255) aacgccaacgccCAGCTCCGGTAAGCAACGTGGGGCATCCGGGGCCATTGGCAGGTAATCAATCATGCCGTTGCGGCGGGCCCACCGACC‑GAGGCAGAGTTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACcgc < 2:940047/150‑1 (MQ=255) aaCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGTAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGGTTTTCGCCACCGTCAGCCCCTTCCCCACACATAACTCCTGATAAACcgc > 1:323991/1‑150 (MQ=255) aaCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCTCTTCCACCAGACTTAACTCCTGATAAACcgc < 1:114452/150‑1 (MQ=255) aCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACTGACC‑CAGGCAGAGGGTTTTCGCCCCTGTCCGCCCTTCCCCCACACTTAACTCCTTATAAAAcccgc > 1:800046/1‑151 (MQ=255) aCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACC‑CAGGCGGAGGGTTTTCGCCCCCGGCCGCCCCTCCCCCAGACCTAACACCTGATaaa > 2:464768/1‑145 (MQ=255) cGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACcgcgcg < 1:224684/151‑1 (MQ=255) cGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACcgcgcg < 2:259521/151‑1 (MQ=255) tCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACCGACC‑GAGGCAGAGGGTTTTCGCCCCCGTCAGCCCCTCCCCCAGACTTTACTCCTGATAAACcgcgc > 2:1116955/1‑148 (MQ=255) cAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCAc < 1:863084/151‑1 (MQ=255) aaCGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCTGACC‑GAGGCAGAGGTTTTTCGCCACTGTCAGCCCCTCCCCCCCACTTAACTCCTGATAAAACGCGCGcccc > 2:1146181/1‑151 (MQ=255) cAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGt < 2:1054037/151‑1 (MQ=255) cgcCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACC‑GCGGCCGAGGGTTTTCGCCACTGTCAGCCCCTCCCCCCGACTTAAATCCTGATAAAACGCGCGCACCCCCCGtt > 2:77794/1‑149 (MQ=255) gcCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACC‑GAGGCAGAGGTTTTTCGCCCCTGTCAGCCCCTCCACCCGACTTAACTCCTCTTAAACCGCGCGCACCCCCacatt > 1:216782/1‑145 (MQ=255) tGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGAAC‑GAGGGAGAGGGTTTCCCCCCCTGCCAGCCCCTCCACCCCACTTAACTCCTGATAAAACACGCCCGCCCCCCGATCAGCgg > 1:62576/1‑151 (MQ=255) aaGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACC‑GAGGGAGAGGGTTTTCCCCCCTGTCAGCCCCTCCACCAGACTTAAATTCTGATAAAACCCGCGCACCCCCCCGTCAGCGGCa > 1:50956/1‑150 (MQ=255) aaCGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCg < 1:865100/151‑1 (MQ=255) ttGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGGAGAGGGTTTTCGCCCCTGTCAGCCCCTCCCCCCGACTTAAATCCTGATAAAACGCGCGCACCCCCAGGTCAGCGGCACGGCg > 1:680881/1‑147 (MQ=255) tGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCCACC‑CAGGGAGAGGGGTTCCTCCACCGTCACCCCCTCCACCCGAATTAACTCCCGTTAAAACCCCCGCCCCCCCCGTTCACCGGCACGCCggggcc > 2:592041/1‑146 (MQ=255) gCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACCGACC‑GAGGCAGAGGGTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGCTAAAACCCGCGCCCCCCCCGTTCAGCGGCACGGCGa > 1:615485/1‑144 (MQ=255) aTCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACC‑CGGGCGGAGGGTTTTCGCCACTTTCCGCCCCTTCCCCAGCCTTAACTCCTGATAAAACGCGCGCCCCCCCACGTCCGcggcgcggcggggca > 2:1118508/1‑141 (MQ=255) tCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACC‑CAGGCAGAGGGTTTTCGCCACTGTCAGCCCTTCCACCCGACTTAACTCCTGATAAAACCCGCGCACCCCCCGTTCAGCGGCACGGCGGGTCAg > 2:561485/1‑146 (MQ=255) gcgcCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTCAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTc < 2:1008469/151‑1 (MQ=255) cgcCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTc < 2:230977/150‑1 (MQ=255) cgcCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCg < 1:690127/151‑1 (MQ=255) cgcCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCg < 2:494063/151‑1 (MQ=255) cATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCAACC‑CAGGGCGAGGGTTTTCGCCCCTGTCCGCCCTTCCCCCCGAATTAACTCCTTATAAAACCCGCGCACCCCCCCCTCAGTGGCCCggggggcacacgga > 2:773199/1‑130 (MQ=255) cATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGGTTTTCGCCACTGTCAGCCCTTCCCCCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGCAGCTTCGTc > 1:410686/1‑150 (MQ=255) ttGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCGCTTACC‑GGGGGAGAGGGTTTTCGCCACCGTCAGGCCTTCCCCCAAACTTAAATCCTGCTAAACCCCGCGCACCCCCCCTTCCGCGGCACGCCGCGTCAGCGGAGCTTCGTcccc > 2:707659/1‑150 (MQ=255) tGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCt < 1:437490/150‑1 (MQ=255) gCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACC‑GAGGCAGAGGGTTTTCCCCCCTGTCAGCCCTTCCCCCCGACTTAACTCCTGTTAAAACGCGCGCACCCCCCCTTTAGCGGCACGGCGAGGCAGCCGAGCTTCGTCACCtt > 1:95984/1‑150 (MQ=255) aatcaatcATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTc < 2:710758/150‑1 (MQ=255) cggcggGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCCTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATcc > 1:892613/1‑148 (MQ=255) ggcggGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGa < 2:23978/150‑1 (MQ=255) gcggGGCCACTGCCC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGat < 2:189384/150‑1 (MQ=255) ggggCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATc > 1:874150/1‑151 (MQ=255) gggCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATc > 1:5553/1‑150 (MQ=255) gggCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCa < 1:1147691/151‑1 (MQ=255) gACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTa < 2:723896/149‑1 (MQ=255) gACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATc > 1:454274/1‑151 (MQ=255) c‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCgg > 1:248448/1‑151 (MQ=255) agGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGc < 1:587487/151‑1 (MQ=255) | CCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACC‑GAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGC > NC_000913/2677890‑2678186 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |