Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,678,039	0	T	G	44.1%	85.5 / 15.0	62	N108T (AAC→ACC)	yphE	putative sugar ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (7/26);  new base G (26/0);  total (36/26)
Fisher's exact test for biased strand distribution p-value = 1.12e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCT  >  NC_000913/2677890‑2678187                                                                                                                                                       |                                                                                                                                                      ccaGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGt                                                                                                                                                       <  2:609840/150‑1 (MQ=255)  caGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGCCACCGACCGAGGCAGA‑GGgttt                                                                                                                                                    >  2:559568/1‑151 (MQ=255)       gctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCTGACCGAGGCGGA‑GGgttt                                                                                                                                                    >  1:1134562/1‑147 (MQ=255)       gctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTcc                                                                                                                                                  <  2:395237/149‑1 (MQ=255)       gctATTGCGTCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTATCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGGATCCTGCGCCATTGGCAGGAAATAAAGCATGCCGTTGCGGCGGGGCCACTGCCCGAGGCAGA‑GGTTTTcc                                                                                                                                                  <  1:31920/149‑1 (MQ=255)        ctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCTGACCCAGGCAGAGGGTTTTcccc                                                                                                                                                >  2:30126/1‑149 (MQ=255)        ctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGc                                                                                                                                                <  1:819871/150‑1 (MQ=255)         ttttGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACCGACCGAGGCAGA‑GGGTTTCCGcc                                                                                                                                               >  1:55997/1‑150 (MQ=255)             gcgcCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCCCGGCAGAGGGTTTTCccccccg                                                                                                                                            >  1:308903/1‑147 (MQ=255)               gcCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCGCCGACCGCGGGAGG‑GGGTTTTCGCCAccctc                                                                                                                                         >  1:133077/1‑146 (MQ=255)                 cGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGAGCCGCCTAACACAGCAGA‑AGATTTCCGCCaacgcttac                                                                                                                                      >  1:321171/1‑143 (MQ=255)                   ggCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGGGGCAGA‑GGTTTTCCGCCACTGTC‑AGccc                                                                                                                                   >  2:306675/1‑151 (MQ=255)                      ttAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCCGACCGAGGCAGA‑GGGTTTTCGCCACCGTC‑AGCCCtt                                                                                                                                 >  1:440643/1‑150 (MQ=255)                         gCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTGACCGAGGCAGA‑GGGTTTCCCCCACTGTC‑AGCCCTTccac                                                                                                                             >  2:523759/1‑151 (MQ=255)                         gCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTcca                                                                                                                              <  1:497597/150‑1 (MQ=255)                         gCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCGAGGCAGA‑GGGTTTTCGCCCCCGTC‑AGCCCCTccac                                                                                                                             >  2:166662/1‑151 (MQ=255)                               aaaCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGGTTTCCGCCCCTGTC‑AGCCCTTCCACCAGAc                                                                                                                        >  2:1028533/1‑150 (MQ=255)                                aaCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCCAACCGAAGGAGA‑AGAATTTTTCCACTGTC‑GGGCCCTCCACCCCACtt                                                                                                                      >  1:372169/1‑151 (MQ=255)                                  cAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTaa                                                                                                                    <  2:535474/151‑1 (MQ=255)                                  cAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGGCCCAGGCAGA‑GGGTTTTCCCCCCTGTC‑AGCCCGCCCACCAGACt                                                                                                                       >  2:452936/1‑148 (MQ=255)                                   aaGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAAc                                                                                                                   <  2:751071/151‑1 (MQ=255)                                    aGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCGGGGGAGA‑GGGTTTTCCCCGCCGTC‑AGCCCCTCCACCCGACt                                                                                                                       >  2:69027/1‑146 (MQ=255)                                     gttgttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACCGCACCAGGGAGA‑GGGTTTTCGCCCCTGTC‑AGCCCCTCCCCCCAACttca                                                                                                                    >  2:847730/1‑146 (MQ=255)                                       tgttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCCGACCGAGGAAGG‑GGGTTTCCGCCCCCGTC‑AGCCCCTCCACCAAAATTAAcctc                                                                                                                >  2:1239694/1‑147 (MQ=255)                                        gttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTg                                                                                                              <  1:390248/151‑1 (MQ=255)                                        gttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGAGCCCCCGACCCAGGCCGA‑TGGTTTTCGCCGCTGTC‑CGCCCCTccacc                                                                                                                            >  1:1206162/1‑137 (MQ=255)                                          tCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCGAGGGAGA‑GGGTTTTCGCCACCGTC‑AGCCCCTCCACCAGACTTTACTCCTGAt                                                                                                            >  2:460119/1‑151 (MQ=255)                                          tCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGCGGCAGA‑GGGTTTTCGCCACCGTC‑AGCCCTTCCCTCAGAATTAACTcc                                                                                                                >  2:634168/1‑147 (MQ=255)                                                  aaCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTGACCGAGGCAGA‑GGGTTTTCGCCCCCGTC‑AGCCCCTCCCCCCGACCTAACTCCCGGTAAAAcg                                                                                                      >  1:577291/1‑149 (MQ=255)                                                       cAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTTACCCAGGCAGA‑GGGTTTCCGCCACTGTC‑CGCCCTTCCCCCAGACTTAAATCCTGATAAAAcccgc                                                                                                   >  2:603547/1‑147 (MQ=255)                                                           gCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCCAGGGAGA‑GGGTTTTCGCCCCCGTC‑CGCCCCTCCACCCCACCTAACTCCTGATAAAACCCGCGCAccccc                                                                                           >  1:871870/1‑151 (MQ=255)                                                           gCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCAccccc                                                                                           <  2:704038/151‑1 (MQ=255)                                                            cccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCa                                                                                          <  1:987387/151‑1 (MQ=255)                                                            cccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCCCGGGAGA‑GGGTTTCCGCCACTGTC‑AGCCCCTCCCCCCGCCCTAAATCCCCATAAACCCCGCGCccccccc                                                                                          >  1:234319/1‑150 (MQ=255)                                                             ccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACCGAATGGGGGAGA‑GGGTTTTCGCCACTGTC‑GGGTCCTCCACCCAACTTAACTCCTGTGAAAACCCGCGCAacccc                                                                                           >  1:881717/1‑149 (MQ=255)                                                             ccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAg                                                                                         <  1:260785/151‑1 (MQ=255)                                                                  gcCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTGACCGCGGCAGA‑GGGTTTTCCCCACTGTC‑AGCCCCTCCACCCAACTTAACTCCCGATAAAACGCGCGCACCCCCacgtc                                                                                      >  2:778817/1‑145 (MQ=255)                                                                   ccTGTAAGCAACGTTGGGCATCCTGCGCCATGTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACGGACCGAGCCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGc                                                                                   <  2:1105573/151‑1 (MQ=255)                                                                    cTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCCACCGAGGGAGA‑GGGTTTTCGCCCCTGTC‑AGCCCTTCCCCCCGACTTAACTCCCTATAAAACGCGCGCccccccccctc                                                                                      >  1:749805/1‑142 (MQ=255)                                                                       tAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCCCGGCCGA‑GGGTTTCCGCCCCTCTC‑ACCCCTTCCCCCAGACCTTACTCCCGATAAACCCCCCCCCCCCCCCATTCACCg                                                                                  >  1:158729/1‑148 (MQ=255)                                                                       tAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCCACCGCGGCGGA‑GGGTTTTCGCCCCTTTC‑CGCCCCTCCCCCCGACCTTACTCCTTATAAAACCCGCGCACCCCCCCTTCAGccg                                                                                 >  1:2361/1‑147 (MQ=255)                                                                            aaCGTTGGGCATCCTGCGCCATTTGTAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCg                                                                          <  1:1152468/151‑1 (MQ=255)                                                                               gTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTGGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGa                                                                         <  2:787781/149‑1 (MQ=255)                                                                                   ggCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTTACCCGAGCAGA‑GGGTTTCCCCCACTCTC‑AGCCCTTCCCCCAGACCTAAATCCTGATAAAACCCGCGCCCCCCCCCTTCAACGGGACAGCGAGTCa                                                                     >  2:1228995/1‑149 (MQ=255)                                                                                    gCATCCTGCCCCATTTGCAGGTAATCAATCATGCCGGTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCg                                                                  <  2:144025/151‑1 (MQ=255)                                                                                         cTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTGGCCGAGGCAGA‑GGGTTTTCTCCACCTTC‑CGCCCCTCCACCAGACTTAAATCCTGATAAACCCCCCGCACCCCCCGGTCCGCGGCACCGCGGGGCAGCGGAg                                                               >  2:899991/1‑149 (MQ=255)                                                                                               cATTTGCAGGTAATCAATCATGCCGTTGCGGCGGCGCCACTCATTAAGGCAGA‑GGCTTTCCCCGACTCTCTGGCCCTTCC‑CCAGCGTTCACACCTGATTAATCCCGTGCACCCCCAACTCAACGGCCAGGCGAGTCCGcagatatttg                                                          >  2:1158529/1‑139 (MQ=255)                                                                                                 tttGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGCGGGGGA‑GGGTTTTCGCCCCTCTC‑AGCCCCTCCCCCAGACCTAACTCCTGATAAAACCCGCGCCCCCCCCCTTCAGCGGCACGGCGGGGCAGCGGAGCCTCGTcccc                                                     >  1:1060357/1‑151 (MQ=255)                                                                                                   tGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCt                                                    <  2:1163329/150‑1 (MQ=255)                                                                                                      aGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCa                                                <  2:317966/151‑1 (MQ=255)                                                                                                         tAATCAATCATGCCGTTGCGGCGGGGGCACTGACCGAGGCAGA‑GGGTTTTCGCCACTGTC‑AGCCCCTTCACCCGACTTAAAACCCGATAAACCGCGCGCACCCCCCCTTCAGCCGCACGGCGAGGCAGGGGAGATTCGTTCCCTTcccctc                                             >  2:812239/1‑147 (MQ=255)                                                                                                           atcaatcaTGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTcgc                                           <  1:325493/151‑1 (MQ=255)                                                                                                           atcaatcaTGCCGTTGCGGCGGGGCCACCGACCGCGGCAGA‑GGTTTTCCCCCCCTGTC‑AGCCCCTCCACCAGACTTTACTCCTGGTAAACCCCCCGCACCCCCAGTTCAGCGGCACGGCGCGTCAGCGTAGCTTCGTCACCTTCCAGTcgc                                           >  2:221012/1‑151 (MQ=255)                                                                                                                  aTGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGc                                    <  2:829675/151‑1 (MQ=255)                                                                                                                    gcggTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGtct                                       <  1:1124109/143‑1 (MQ=255)                                                                                                                            ggcggGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATcc                              >  1:1098154/1‑147 (MQ=255)                                                                                                                              cggGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGGC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGatat                        >  1:378660/1‑151 (MQ=255)                                                                                                                                gggCCACTGACCTAGGCGGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGCCTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCATCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCa                      <  2:1082364/151‑1 (MQ=255)                                                                                                                                   ccACTGACCGAGGCAGA‑GGTTTTCCGCCTCTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCAcct                   <  1:980361/151‑2 (MQ=255)                                                                                                                                      cTGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCt                 <  2:1150481/150‑1 (MQ=255)                                                                                                                                       tGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTAt               >  2:816105/1‑151 (MQ=255)                                                                                                                                               gCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGt       <  2:760283/151‑1 (MQ=255)                                                                                                                                                 aga‑gGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGtt      <  1:420507/150‑1 (MQ=255)                                                                                                                                                 aga‑gGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTc     <  1:30125/151‑1 (MQ=255)                                                                                                                                                     ggTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCt  <  1:1124839/151‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                      CCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGA‑GGTTTTCCGCCACTGTC‑AGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCT  >  NC_000913/2677890‑2678187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑