Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,492,834	0	T	G	43.3%	98.0 / 17.3	68	G89G (GGT→GGG)	tsgA	putative transporter
Reads supporting (aligned to +/- strand):  ref base T (10/28);  new base G (29/0);  total (40/28)
Fisher's exact test for biased strand distribution p-value = 1.15e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACATTCCTGGTAACACAAATGTATGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACTCC  >  NC_000913/3492686‑3492984                                                                                                                                                     |                                                                                                                                                         tGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGgtt                                                                                                                                                        >  1:1096326/1‑151 (MQ=255)  gCCTGTTTCCAGTATGAGTAATACCATCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCGTTCTCCTGATGGTGCTGGCGGTTGCCGGtg                                                                                                                                                         <  1:378640/149‑2 (MQ=255)      gTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATg                                                                                                                                                    <  2:558486/150‑1 (MQ=255)      gTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATg                                                                                                                                                    >  1:263896/1‑150 (MQ=255)       tttCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTTTTGtt                                                                                                                                                  >  1:784379/1‑151 (MQ=255)          ccAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCa                                                                                                                                                <  2:557394/150‑1 (MQ=255)             gTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGCTGCCGGGTTTGTGtttaatc                                                                                                                                             >  2:1150457/1‑145 (MQ=255)                 gAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAg                                                                                                                                         <  1:975551/150‑1 (MQ=255)                 gAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGCTTTGTGTTTAGc                                                                                                                                              >  1:935283/1‑145 (MQ=255)                  cgTAATACGTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTAATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGGTTTGTGTTTAGcccgagg                                                                                                                                        >  1:313064/2‑145 (MQ=255)                  aGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGGTTTAATTTCAGCCCCAccc                                                                                                                                       >  1:573899/1‑151 (MQ=255)                    tAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGTGGTTTCGCCCCAGcc                                                                                                                                       >  2:184083/1‑149 (MQ=255)                      aTACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGGTTGTTGGTTAGCCCCAACCTgg                                                                                                                                    >  2:963066/1‑150 (MQ=255)                           ttcaccttcCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGGTTTATTTTTCGCCCCAGccctg                                                                                                                                    >  2:1142002/1‑142 (MQ=255)                           ttcaccttcCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGGTGCCGGGTTGGTGGTTTGCCACTGCCTGgctc                                                                                                                                 >  2:535444/1‑146 (MQ=255)                                    cTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGGTTGTTGTTCAGGCACAACCTCGGGCTGTTCTcggcg                                                                                                                      >  1:990465/1‑150 (MQ=255)                                      cAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTcggcgg                                                                                                                     <  2:914819/149‑1 (MQ=255)                                                aTTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGGTTTTTGTTTTGCCCCACCCTGGCGCTGTTCTCTGCGGCGGTGTTCAtt                                                                                                          >  2:410994/1‑150 (MQ=255)                                                aTTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGGTTTATGTTTAGCCACAGCCTGGCGCTGTTCTCGGCGGCGGTGTTCAtt                                                                                                          >  2:350248/1‑150 (MQ=255)                                                 ttttAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGGTTTTTGTTTAGCCACAGCCCGGGGCTGTTCTCGGCGGCGATGTTTATtct                                                                                                        >  2:372571/1‑151 (MQ=255)                                                   ttAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATtct‑c                                                                                                      <  2:341497/150‑1 (MQ=255)                                                    tAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATtct‑c                                                                                                      <  2:183359/149‑1 (MQ=255)                                                      aTCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGGTTTGTGTTCAGCCACCGCCTGGCGCCGTTTTCGGCGGCGGTGTTTATTTT‑Cgggg                                                                                                  >  2:581151/1‑151 (MQ=255)                                                       tctcTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGCTGTCGGGTTGGTGTTTAGCCCCAGCCCGGCGCTTTTTTCGGCGGCGGTGTTTATTTT‑Cgggg                                                                                                  >  1:865219/1‑150 (MQ=255)                                                          ctATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGggt                                                                                                 <  1:1000857/148‑1 (MQ=255)                                                           tATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCCGGTTGTTGTTCCGCCCCAGCCTCGGGCCGTTTTTGGCGGGGGTTTTTTTTTT‑CGgggggg                                                                                               >  2:236107/1‑149 (MQ=255)                                                           tATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTCGCCGGGTTTATGTTTTGCCCCAGGCCGGCGCCGTTCTCGGCGGGGGTGTTCATTTT‑CTgggggggg                                                                                             >  2:490255/1‑149 (MQ=255)                                                           tATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGATGACGGGTTTATGTTTATGCCCAGCCTTGCGCTGTTTTTGggggcgtggttttttct                                                                                                        >  1:303941/1‑130 (MQ=255)                                                                  cTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCg                                                                                         <  2:558302/148‑1 (MQ=255)                                                                  cTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGGTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGGGGTGTTCATTCT‑CGGGGGGGTCAGCgg                                                                                        >  2:795682/1‑149 (MQ=255)                                                                     aaCGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGGTTTATGTTTTGCCACAGCCCGGCGCTGTTTTCGGGGGGGGTGGTTATTTT‑CCGGGGGGGCCGCGGGATc                                                                                    >  2:972806/1‑150 (MQ=255)                                                                      aCGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCAc                                                                                  <  1:697546/151‑1 (MQ=255)                                                                      aCGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGGTTGGTGTTTAGCCACAACCCGGCGCCGTTCTCGGCGGCGGGGTTTATTTT‑CGGGGGGGGGAGCGGcgt                                                                                     >  1:1042838/1‑146 (MQ=255)                                                                            ggCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTTCCGGGGTGTTGGTTCGCCCCAACCCGGCGGTGTTTTCGGCGGCGGAGTTTTTTTT‑CGgggggggccagggcgacacc                                                                                 >  1:47581/1‑132 (MQ=255)                                                                            ggCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATg                                                                              <  1:1141360/149‑1 (MQ=255)                                                                            ggCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGt                                                                             <  1:1203454/150‑1 (MQ=255)                                                                            ggCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGGTTTATGTTTAGGCCCAACCCGGCGCCGGTCTCGGCGGCGGTGGTCATTTT‑CGGGGGGGGCAGCGGGATCACCATg                                                                              >  2:1162293/1‑149 (MQ=255)                                                                               tGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGGTTTGTGTTTAGCCCCAGCCTGGCGCTGTTCTCGGCGGGGGGGTTCATTCT‑CGGGGGGGGGAGCGGCATCACCATGTCGA‑t                                                                        >  1:1053718/1‑151 (MQ=255)                                                                                gATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGa                                                                          <  2:588462/149‑1 (MQ=255)                                                                                gATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑tt                                                                       <  2:753719/151‑1 (MQ=255)                                                                                    gAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGGCGGGTTTATGTTTAGCCCCCACCCGGCGCTGTTTTCGGGGGCGGTGGTCATTTT‑CGGGGGGGGCCGCGGCATCCCCCAGTCGG‑tttg                                                                     >  1:537916/1‑147 (MQ=255)                                                                                       aTCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACAt                                                                <  2:47582/151‑1 (MQ=255)                                                                                          gTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGGTTTATGTTTAGCCCCAGCCCGGGGCTGTTCTCGGCGGGGAGGTTTATTTT‑CGGGGGGGGCAGCGGCATCACCCTGTCGG‑TTGGGACAtttc                                                             >  2:46020/1‑149 (MQ=255)                                                                                             ccGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTTCCGGGTTTATGTTTCGCCCCAACCTGGCGCTGTTCTCGGCGGGGGTGTTCATTCT‑CGGGGGGGGCCGCGGCATCACCATGTCGG‑TTGGTACATTcc                                                             >  2:166121/1‑148 (MQ=255)                                                                                               gTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACATTCCTGGTa                                                        <  1:485582/151‑1 (MQ=255)                                                                                               gTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGGTTGTTGTTTAGCCCCACCCTGGGGCCGTTTTCGGCGGCGGTGTTCATTTT‑TGGGGGGGGCCGCGGGCTCACCCTGTGGG‑TTTGTACAtttc                                                             >  1:802199/1‑144 (MQ=255)                                                                                                ttGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTTGCGGTTTTGTTTTAAGCCAAACCCCGGCGCCGGTCTTCGCGGCGGTGTTTATTTT‑CTGGGGGGGCAGGGGCAGCAACAAATCGG‑TTGGGATAtt                                                               >  2:1006177/1‑143 (MQ=255)                                                                                                   aaaaCGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACATTCCTGGTAacac                                                    <  1:1051861/151‑1 (MQ=255)                                                                                                     aaCGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACATTCCTGGTAACACaa                                                  <  2:1135004/151‑1 (MQ=255)                                                                                                           gTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACATTCCTGGTAACACAAATGTAt                                            <  1:803997/151‑1 (MQ=255)                                                                                                           gTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACATTCCTGGTAACACAAATGTAt                                            <  2:823821/151‑1 (MQ=255)                                                                                                            ttACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTTCCGGTTTGCTGTTCACCCCCAGCCTCGCGCTGGTCTCGGCGGCGGAGTTCCTTTTCCGGGGGGGTCTCGGGCA‑CACCATGTCGG‑TTGGTACCTCCCCTGGAAAACAAATgaa                                             >  2:272096/1‑147 (MQ=255)                                                                                                                gTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACATTCCTGGTAACACAAATGTATGAAgg                                       <  1:826291/151‑1 (MQ=255)                                                                                                                 ttttGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGGTTTATGTTTAGCCACAGCCTGGCGCTGTTTTCGGCGGGGATGTTTATTTT‑CCGGGGGGGCAGCGGCATCACCATGTCGG‑TTGGGGCATTCCTGGTAACACAAAAGTATGAAggg                                      >  2:749654/1‑151 (MQ=255)                                                                                                                  tttGGCTTTCTCCTGATGGTGCTGGCGGTTGGCCGTTTGATTTTCAGCCACAGACTGGGGCTGTTCTCGGCGGCGGTGTTCATTTT‑CGGGGGGGTTAGCGGCATTACCATGTCGA‑TTGGGACAATCCCGCTAACACaaaaagatgtaggg                                      >  1:350527/1‑139 (MQ=255)                                                                                                                   ttGGCTTTCTCCTGATGGTGCTGGCGGTTTCCGGGTTTATGTTTAGCCAAAACCTGGGGCTGTTCTTCGGGGGGGTGTTCATTTT‑CTGGGGGGGCAGAGGGCGCACCCTGTTGA‑GTGGGATATTCCCGGGAAAACAAATGTATGAAggg                                      >  2:265933/1‑149 (MQ=255)                                                                                                                   ttGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACATTCCTGGTAACACAAATGTATGAAGGGc                                     <  1:977317/150‑1 (MQ=255)                                                                                                                       cTTTCTCCTGATGGTGCTGGCGGTTGGCGGGTTGATGTTTTACCACAGCCTGGCCCTGTTCTCGGCGGGGATGGTCCTTTC‑CGGGGGGGGCAGCGGCATCACCATGTGGA‑TTTGTGCATTTCTGGTAACACAAATGTATGAAGGGCGtta                                 >  2:976996/1‑148 (MQ=255)                                                                                                                           ctcCTGATGGTGCTGGCGGTTGGCGGTTTGGTGTTCAGCCACCCCCTCGCGCCTGTCTCGGCGGCGGTGGTTATTTT‑CCGGGGGGGGCGCGGCCTCCCCACGCCCATTTGGTCAATTCCCGG‑AACACCAAACTATGAAAGGGGTCCgcgc                             >  1:1022037/1‑150 (MQ=255)                                                                                                                           ctcCTGATGGTGCTGGCGGTTGCCGGGTTTATGTTCAGCCACAGCCTGGCGCTGTTTTCGGCGGGGATGTTCATTTT‑CGGGGGGGGCCACGGCATCACCATGTCGA‑TTGGGACATTCCTGGGAAAACAAAAGTATGAAGGGCGTCAgcg                              >  2:984787/1‑149 (MQ=255)                                                                                                                             ccTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACATTCCTGGTAACACAAATGTATGAAGGGCGTCAgcgc                             <  2:1090595/148‑1 (MQ=255)                                                                                                                                 aTGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACATTCCTGGTAACACAAATGTATGAAGGGCGTCAGCGCGGTTccc                      <  2:271993/151‑1 (MQ=255)                                                                                                                                      gCTGGGGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACATTCCTGTTAACACAAATGTATGAAGGGCGTCATCGCGGTTCCCGCCt                  <  1:800454/150‑1 (MQ=255)                                                                                                                                         ggCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACATTCCTGGTAACACAAATGTATGAAGGGCGTCAGCGCGGTTCCCGCCTGtt               <  2:58216/150‑1 (MQ=255)                                                                                                                                          gCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCG‑CGGGGTGGTCAGCGGCATCCCCATTTCGA‑TTGTTACATTCCTGGTAACACAAATGTATGAAGGGCGTCAGCGGGTTTCCCGCCTGTTAt             <  1:184079/151‑1 (MQ=255)                                                                                                                                              ttGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACATTCCTGGTAACACAAATGTATGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTa          <  2:1096388/150‑1 (MQ=255)                                                                                                                                               tGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACATTCCTGGTAACACAAATGTATGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTAc         <  2:1238438/150‑1 (MQ=255)                                                                                                                                                 ccGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACATTCCTGGTAACACAAATGTATGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCg       >  1:1063454/1‑150 (MQ=255)                                                                                                                                                    gTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACATTCCTGGTAACACAAATGTATGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACTc   >  2:575590/1‑151 (MQ=255)                                                                                                                                                    gTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACATTCCTGGTAACACAAATGTATGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACTc   >  2:937214/1‑151 (MQ=255)                                                                                                                                                     tttGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACATTCCTGGTAACACAAATGTATGAAGGGCGTTAGCGCGGTTCCCGCCTGTTATTTACCGACTcc  >  1:900708/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                         TGCCTGTTTCCAGTATGAGTAATACCTTCACCTTCCTCAACGCCGGCATTTTAATCTCTATCTTCCTCAACGCCTGGCTGATGGAAATCGTCCCGTTGAAAACGCAGTTACGTTTTGGCTTTCTCCTGATGGTGCTGGCGGTTGCCGGTTTGATGTTCAGCCACAGCCTGGCGCTGTTCTCGGCGGCGATGTTCATTCT‑CGGGGTGGTCAGCGGCATCACCATGTCGA‑TTGGTACATTCCTGGTAACACAAATGTATGAAGGGCGTCAGCGCGGTTCCCGCCTGTTATTTACCGACTCC  >  NC_000913/3492686‑3492984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑