Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	29,496	0	T	A	45.2%	80.9 / 49.4	73	intergenic (+301/‑155)	dapB/carA	dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (30/10);  new base A (0/33);  total (30/43)
Fisher's exact test for biased strand distribution p-value = 3.71e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGG  >  NC_000913/29347‑29646                                                                                                                                                       |                                                                                                                                                        ttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                                         <  1:987557/150‑1 (MQ=255)  tAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAAtata                                                                                                                                                        >  1:746161/1‑150 (MQ=255)   aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaa                                                                                                                                                       <  1:65442/150‑1 (MQ=255)      gTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaaa                                                                                                                                                    >  2:594816/1‑150 (MQ=255)       tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                                  >  2:376845/1‑151 (MQ=255)        aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑c                                                                                                                                                <  2:672870/151‑1 (MQ=255)         aaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑cc                                                                                                                                               <  1:700419/151‑1 (MQ=255)            tttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCg                                                                                                                                             <  2:1192871/150‑1 (MQ=255)            tttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCg                                                                                                                                             <  1:210845/150‑1 (MQ=255)            tttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGc                                                                                                                                            >  1:793570/1‑151 (MQ=255)             ttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGc                                                                                                                                            <  2:284594/150‑1 (MQ=255)                  cATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTa                                                                                                                                       >  2:284528/1‑150 (MQ=255)                     ttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGtt                                                                                                                                   >  1:1160093/1‑151 (MQ=255)                       ggcccACTTTTTTCTGCTCGTTTTTTTTTCATGCAATTTTCTTGCTGCGCAAGCGTTTTCC‑AAAACAGGTTAGATGATTTTTTTGTCGCTTAATGCCTGAAAAACATGCATGAGCCAAAAAATAATATAAAAAAT‑CCCGCCATTAAGtt                                                                                                                                   <  2:269181/146‑1 (MQ=255)                          ccttttttttCTGCTTTTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCTTTTCCC‑AGAACAGTTTAGATGATTTTTTTGTCGCTTAATCCTTGAAAAACATGCATGACCCAAAAAATAATAAAAAAATT‑CCCGCCATTAAGTTGACtt                                                                                                                              <  2:217083/147‑1 (MQ=255)                               tttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAgc                                                                                                                         >  2:271735/1‑151 (MQ=255)                                ttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTa                                                                                                                           >  1:1141324/1‑148 (MQ=255)                                             tttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                             >  1:377006/1‑149 (MQ=255)                                              ttttttCATTCATTCTTCTTCCTACGCAGCCTTTTACA‑AAAACGAGTAAGATGATTTTTTTGTCGCTTAATCCCTAAAAACCATGCTTGACCCACAAAAAAATAAAAAAAAC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAg                                                                                                           <  1:917520/147‑1 (MQ=255)                                                aTTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                                        >  2:63443/1‑151 (MQ=255)                                                         aaTCTTCTTGCTGCGCAAGCTTTTCCA‑AAAACAGTTTAGATGTTTTTTTTGTCGTTTAATCCCTGTAAAACATGCATGACCCAAAAAATAATAAAAAAATT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATg                                                                                                       <  1:1055765/143‑1 (MQ=255)                                                         aaTCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATACCGCCGtt                                                                                               >  2:745213/1‑151 (MQ=255)                                                          aTCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                              >  1:2670/1‑151 (MQ=255)                                                           tttttttGCTGCGCAAGCTTTTTCA‑AAAACAGGTTAAATGTTTTTTTTGCCTTTAATTGCTTGAAAAAAATGCATGACCCAAAAAAAAATAAAAAAAAC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                                  <  2:393749/141‑1 (MQ=255)                                                             ttcttGCTGCGAAAGCTTTTTCA‑AAAAAAGGTAAAATGATTTTTTTGTCCCTTAATGCTTAAAAAACATGCATGACCCAAAAAATAAAAAAAAAACC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGc                                                                                            <  1:103299/150‑1 (MQ=255)                                                                    tgcGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                                    >  2:1053186/1‑151 (MQ=255)                                                                    tgcGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                                    >  2:1013666/1‑151 (MQ=255)                                                                     gcgcAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                                   >  1:103754/1‑151 (MQ=255)                                                                      cgcAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcg                                                                                  >  1:240985/1‑151 (MQ=255)                                                                        cAAGCTTTTACA‑AAAACAGTTAAGATGATTTTTTTTTCGCTTAATCCCTTAAAACCATGCAGGCCCAAAAAAAAAAAATAAAAACT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                                       <  1:85169/144‑1 (MQ=255)                                                                         agggttttaCC‑AAAAAAGTTTAAATGTTTTTTTTGTGGTTTAATGCCTAAAAAACATGGATGGGCAAAAAAAAAAAAAAAAAAAC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                                    <  2:746166/141‑1 (MQ=255)                                                                         aaGCTTTTTCC‑AGAACGGTTTAGATGTTTTTTTTGTCGTTTAATGCCTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAt                                                                                     <  2:977980/145‑1 (MQ=255)                                                                         aaGCTTTTTCA‑AAAACAGTTTAGATAATTTTTTTGTCGTTTAATGCCTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAAC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtcg                                                                               <  1:531231/151‑1 (MQ=255)                                                                         aaGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCTAGAAATtcgtcg                                                                               >  2:1060776/1‑151 (MQ=255)                                                                              ttttCC‑AGAACAGGTTAGATGATCTTTTTGTCGTTTAATGCCTTTAAAACATGCATGACCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTaa                                                                           <  2:454733/150‑1 (MQ=255)                                                                              ttttCC‑AAAACAGTTTAGATGATTTTTTTGTCGCTTAATGCCTAAAAAACATGCAGGACCCAAAAAAAAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGt                                                                             <  2:289117/148‑1 (MQ=255)                                                                               tttCCAAGAAAAGTTTAGATGATTTTTTTTTCGTTTAATGCCTAAAAAACATGCATGACCCAAAAAATAAAAAAAAAAAC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTaa                                                                           <  1:616808/150‑1 (MQ=255)                                                                               tttCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                                         >  1:949975/1‑151 (MQ=255)                                                                                   caaaaaaagtttAAATTATTTTTTTGTCTTTTATTCCCTAAAAAACATGCATGACCCAAAAAAAAAAAAAAAAAAC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                                         <  1:192830/138‑1 (MQ=255)                                                                                      gAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAttt                                                                   >  2:501339/1‑151 (MQ=255)                                                                                      aaaagggTTAGATATTTTTTTTGTCTTTTAATCCCTAAAAAACATGCAGGACCCAAAAAAAAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAttt                                                                   <  1:1186606/146‑1 (MQ=255)                                                                                        aCAGTTTAGATTATTTTTTTGTCGCTTAATGCCTGAAAAAAATGCAGGACCCAAAAAAAAATAAAAAAACC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGc                                                                 <  1:273084/151‑1 (MQ=255)                                                                                        aCAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGc                                                                 >  2:273664/1‑151 (MQ=255)                                                                                           ggTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                              >  1:96067/1‑151 (MQ=255)                                                                                                gatgatCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAttt                                                         >  2:672329/1‑151 (MQ=255)                                                                                                  ttatttttttGTCGCTTAACGCCTATAAAACATGCATGACCCACAAAATAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAttt                                                         <  1:376844/144‑1 (MQ=255)                                                                                                  tgatCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGTTTTGCATTGATTTa                                                        >  2:587233/1‑150 (MQ=255)                                                                                                     ttttttttTCGTTTAATGCCTAAAAAACTTGCATGACCCAAAAAAAAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                                      <  1:679257/147‑1 (MQ=255)                                                                                                     tttttttGTCTTTAAATGCCTAAAAAACATGCATGAGCCAAAAAATAAAAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAtt                                                          <  1:1034925/143‑1 (MQ=255)                                                                                                       tttttGTCCTTAAATGCTTAAAAACCATGAATGAGCAAAAAAATAAAAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTAc                                                       <  2:1188443/146‑1 (MQ=255)                                                                                                           tGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCAtt                                              >  1:146796/1‑151 (MQ=255)                                                                                                            gTCGTTTAATGCTTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                               <  2:646558/149‑1 (MQ=255)                                                                                                             tCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                                            >  1:903513/1‑151 (MQ=255)                                                                                                              cttttaatccttaaaaaaaaTGAATGACCCAAAAAATAATAAAAAAAAC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAACAGATTTGCATTGATTTACGTCATCATtgt                                            <  1:119800/136‑1 (MQ=255)                                                                                                               gCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                           >  1:707553/1‑150 (MQ=255)                                                                                                                 ttATTGCCTGAAAAACATGCAGGAGCCAAAAAATAATAAAAAAAAC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                                          <  2:799293/149‑1 (MQ=255)                                                                                                                 ttAATGCCTGAAAAACATGCATGACCCAAAAAAAAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                                            <  2:595797/147‑1 (MQ=255)                                                                                                                   aaTGCCTAAAAAACATGCAGGAGACAAAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                           <  1:847986/146‑1 (MQ=255)                                                                                                                       ccTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAAC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTaa                                     <  2:805033/148‑1 (MQ=255)                                                                                                                            aaaaCATGAATGAGCCAAAAAATAAAAAAAAAATG‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa                               <  1:948683/149‑1 (MQ=255)                                                                                                                            aaaaCATGAATGACCCAAAAAAAAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAata                                   <  1:891330/145‑1 (MQ=255)                                                                                                                            aaaaCAGGCATGGACCAAAAAATAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGc                                <  1:255797/148‑1 (MQ=255)                                                                                                                                catgcatgAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa                          >  2:878991/1‑150 (MQ=255)                                                                                                                                  tgcatgAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAag                        >  2:1107995/1‑150 (MQ=255)                                                                                                                                   gcatgACCCAAAAAATAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt                       <  1:584533/150‑1 (MQ=255)                                                                                                                                      taaaacataaaaaaATTAAAAAACA‑CCCGCCATTAAGTTGACTTTTAGCTCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAAATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaaa                         <  1:419930/137‑1 (MQ=255)                                                                                                                                       gacccAAAAAATAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagt                   <  2:586002/147‑1 (MQ=255)                                                                                                                                          ccAAAAAAAAATAAAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagt                   <  2:509982/147‑1 (MQ=255)                                                                                                                                           caaaaaaTAAAAAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat               <  1:609031/148‑1 (MQ=255)                                                                                                                                            aaaaaaaaATAAAAAAAAC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAata              <  1:761795/148‑1 (MQ=255)                                                                                                                                            aaaaaaTAATAAAAAAAAC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt                       <  1:1152419/139‑1 (MQ=255)                                                                                                                                                 ataataTAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTctct        >  1:398788/1‑151 (MQ=255)                                                                                                                                                 ataataTAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTctct        >  2:802271/1‑151 (MQ=255)                                                                                                                                                   aataTAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg      >  2:683834/1‑151 (MQ=255)                                                                                                                                                   aataAAAAAAAC‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTg       <  2:128602/150‑1 (MQ=255)                                                                                                                                                       taAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCGAGAAATTCGTCGGTAAGCAGATTTGCATTGTTTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAggg  >  2:790900/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                        TTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAT‑CCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGGG  >  NC_000913/29347‑29646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑