| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,346,675 | 0 | T | G | 46.0% | 143.9 / 24.8 | 88 | T218P (ACC→CCC) | fumB | anaerobic class I fumarate hydratase (fumarase B) |
| Reads supporting (aligned to +/- strand): ref base T (6/41); new base G (40/0); total (47/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.42e-19 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTCGCAACG‑CAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCGAT > NC_000913/4346527‑4346825 | aaCGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTAc < 2:955992/151‑1 (MQ=255)aaCGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCCGCAGTACCGAGGGGAc > 2:358541/1‑151 (MQ=255) cTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCAGGACCGAGGGGACGCAtt > 1:391041/1‑151 (MQ=255) gACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAAGACCGAGGGGACGCAtttt > 2:827646/1‑151 (MQ=255) aCCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCTGCCGGACCGAGGGGACGCAtttt > 2:792477/1‑150 (MQ=255) ccATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTtct < 2:529222/151‑1 (MQ=255) cATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTtctc < 1:758866/151‑1 (MQ=255) cATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTtct > 2:1105316/1‑150 (MQ=255) ttcgttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGGCGCAGTAACCAGGGGACGCATTTTTTcgacg > 1:405336/1‑151 (MQ=255) tcgttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTcgacg < 2:894627/150‑1 (MQ=255) cgttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCCGGACCGAGGGGACGCAtttttttcac > 2:189085/1‑142 (MQ=255) cgttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTcgacga < 2:917981/150‑1 (MQ=255) cccTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAg < 2:1177874/151‑1 (MQ=255) tCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGtt < 2:650857/149‑1 (MQ=255) gTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGCGCAGTAACCCGGGGACGCATTTTTTTGGCCAGGGAGgtttt > 1:407787/1‑149 (MQ=255) cAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCAGTACCGAGGGGACGCAATTTTTCGAAGAGGGAGTTTTTTA‑Gttt > 2:1125042/1‑150 (MQ=255) aGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTCTCGACGAGGAAGGTTTTCA‑Gttt > 2:177853/1‑149 (MQ=255) ttCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGCGCAGTACCCAGGGGACGCATTTTTTCGACGAGGAAGTTTTTCA‑GTTTGcc > 1:266148/1‑150 (MQ=255) tCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCg < 2:699168/150‑1 (MQ=255) tCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCg < 1:659024/150‑1 (MQ=255) aTCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGa < 1:693065/151‑1 (MQ=255) cGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGt < 1:459393/151‑1 (MQ=255) cGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTACGCAGACGTCCTGCAAATCACAAACGCGATATGGTACGGCGGGCAGGGCGCACGACCCCGGGGGCGCATCTTCTCGACTCGGGAGttttt > 2:320654/1‑136 (MQ=255) gTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGGGCAGCACCGAGGGGACGCATTTTTCCTCCGAGGGAGTTTTTCA‑GTTTGTCggggg > 2:1259158/1‑147 (MQ=255) aaTAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGCCGCAATACCGAGGGGAGGCAATTTTTCGACGGGGAAGGTTTTCA‑GTTTGCCGGGAg > 1:51770/1‑147 (MQ=255) aaTAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCTGGAGGACCCAGGGGAAGCATTTTTTCGACGAGGGAGGTTTTTA‑GTTTGGCgggg > 1:1053322/1‑145 (MQ=255) aaTAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTca < 1:1135950/150‑1 (MQ=255) aGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTcagca‑g < 1:1256212/151‑1 (MQ=255) gTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑gg < 2:1156690/151‑1 (MQ=255) gAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑c < 2:577070/151‑1 (MQ=255) gcgcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCAGTACCGAGGGGACGCATTTTCTCGACGAGGGAGTTTTTTA‑GTTTGGCGGGGGTCAGCA‑ggg > 1:1189663/1‑148 (MQ=255) gcgcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGCGCAGGGCCGGGGGGACGCATTTTTTCGGCGAGGAAGGTTTTTT‑GGTTTCCGGGGGGCAGCC‑GGG‑Gtt > 1:355589/1‑151 (MQ=255) cgcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑Cttt < 1:418522/151‑1 (MQ=255) cTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTTTCGACGAGGAAGTTTTTTA‑GTTTGCCGGGAGGCAGCA‑GGG‑Gttt > 2:996932/1‑149 (MQ=255) gCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGttt < 1:567728/151‑1 (MQ=255) gCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGtt < 1:744478/150‑1 (MQ=255) aaCTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGGAGGAACCAGGGGAAGCATTTTTTCGGCGGGGGAGGTTTTTT‑GTTTGGCGGGGGTCAGCAGGGG‑CTTGGGTTTc > 1:257093/1‑150 (MQ=255) ggTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTag > 2:153188/1‑148 (MQ=255) tttCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATa < 1:334261/150‑1 (MQ=255) tCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACg < 1:758163/150‑1 (MQ=255) tCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGGACCGAGGGGACGCATTTTTTCGACGAGGGAGGTTTTTA‑GTTTGCCGGGGGTCAGCA‑GGG‑GTTTGGTTTCCTGGGAGAGATACg > 2:1337035/1‑150 (MQ=255) cAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTTTCGACGAGGAAGGTTTTTA‑GTTTGCCGGGGGGCAGCA‑GGG‑CTTTGGTTTTCTGGGAGAGATACGtt > 2:458855/1‑151 (MQ=255) aGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGt < 1:276156/149‑1 (MQ=255) aGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCCTTTTTTCGACGAGGGAGGTTTTTC‑GTTTGCCGGGGGGCAGCA‑GGG‑GTTTTGGTTCCTGGGAGAGATACGttt > 1:717682/1‑151 (MQ=255) aGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTCTCGACGAGGGAGTTTTTTA‑GTTTTCCGGGGGGCAGCA‑GGG‑CTTTGGTTTTCTGGGAGAGATACGttt > 2:114864/1‑151 (MQ=255) gttggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTCGGGTACCCCGGGGAAGCACTTTTTCCCCGAGGAACTTTTTTT‑GTTTGCCCGGGGTGAGCA‑GGG‑GTTTGGTTTTCTGGTCGAGGTACGtttt > 2:592449/1‑150 (MQ=255) ggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTttgttg < 2:1335391/151‑1 (MQ=255) gttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTgg < 2:333543/151‑1 (MQ=255) tCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTAACGAGGGGACGCATTTTTTCGACGAGGGAGGTTTTTA‑GTTTGCCGGGGGTCAGCA‑GGG‑CTTTTGTTTTCTGGGAGAGATACGTTttgtt > 2:297204/1‑146 (MQ=255) ccGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTCTCGACGAGGAAGGTTTTCA‑GTTTGCCGGGGGTCAGCA‑GGG‑CGTTGGTTTCCTGGTAGAGATACGTTTTGTTgg > 2:1063704/1‑147 (MQ=255) cGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTTTCGACGAGGAAGTTTTTTA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTtttttt > 2:1201192/1‑144 (MQ=255) gCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTTTCGACGAGGAAGTTTTTTA‑GTTTTCCGGGGGGCAGCA‑GGG‑CTTTGGTTTCCTGGGAGAGATACGTTTTGTTGGCag‑ag > 2:618262/1‑150 (MQ=255) cAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCGGCCGGACCGAGGGGACGCAATTTCTCCACGAGGAAGGTTTTTA‑GTTTTCCGGGGGGCAGCA‑GGG‑GTTTGGTTTTCTGGTAGAGGTAAGGTTTTGTGGCag > 1:921276/1‑147 (MQ=255) aCGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑Agccgcc < 1:101332/151‑1 (MQ=255) cGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTTTCGACGAGGAAGTTTTTTC‑GTTTGCCGGGGGTCAGCA‑GGG‑CTTTTGTTTTCTGGTAGAGATACGTTTTGTTGGCAG‑Agccgcc > 2:759303/1‑150 (MQ=255) gTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCGGCAGTACCGCGGGGAAGCATTTTCTCCACGAGGAAGGTTTTTA‑GGTTGCCGGGGGGCAGCA‑GGG‑GTTTGGGTTTCTGGGAGAGATACGGTTTTTTGGGCG‑Ggccgcc > 1:1325488/1‑149 (MQ=255) tACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGGGCCGAGGGGAAGCATTTTCTCGACGAGGAAGTTTTTCC‑GTTTGGCGGGAGCTAGCA‑GGG‑CTTTTGTTTCCTGGGAGAGGTACGTTTTGTTGTCAG‑Agccgccgcc > 2:685268/1‑151 (MQ=255) aCCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑Agccgccgcc < 2:72699/150‑1 (MQ=255) aCCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCt < 2:937177/151‑1 (MQ=255) aCCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACGCATTTTTTCGACGAGGAAGTTTTTTA‑GTTTGCCGGGGGTCAGCA‑GGG‑CTTTGGTTTCCTGGGAGAGATACGTTTTTTTTGCAG‑Agccgccgcc > 2:874511/1‑150 (MQ=255) ccGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCtt < 2:903977/151‑1 (MQ=255) cGCCAATCACAAACGCGATATGGTACGGCGGACCGGGTGGAAAACCGAGGGCACGTATTTTTTCCGCGTGGGAGTTTTTTACTTTTGCCGGGGGTCGCAA‑GGG‑CTTT‑GTTTTCTCGTTGCGATACGTTTTTTTTGCCGAAGCGGCGGCCtt > 2:643801/1‑151 (MQ=255) gCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTc < 1:216030/151‑1 (MQ=255) ccAATCACAAACGCGATATGGTACGGCGGGCAGGGCGCAGTACCGAGGGGACGCATTTTCTTGACGAGGGAGGTTTTTA‑GTTTGTCGGGGGTCAGCC‑GGG‑GTTTTGTTTTCTGGGAGAGAGACGTTTTTTTGGCCG‑AGCCGCCCCCttt > 1:3346/1‑149 (MQ=255) ccAATCACAAACGCGATATGGTACGGCGGGCAGGCCGCAATACCCAGGGGAAGCATTTTTTTGGCGAGGGAGGTTTTTT‑GGTTTCCGGGGGGCAGCC‑GGG‑GTTTGGTTTTCTGGGAGAGATACGTTTTTTTGGCAG‑AGCCGCCGCCttt > 1:1178694/1‑149 (MQ=255) cAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGAAGCATTTTTTCGACGAGGAAGATTTTCA‑GTTTTCCGGGGGTCAGCA‑GGG‑GTTTTGGTTTCTGGTAGAGATAAGGTTTTTTGGGAG‑AGCCGCCGCCTTTccc > 2:1297857/1‑149 (MQ=255) aaTCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTTTCGACGAGGAAGTTTTTTT‑GTTTTCCGGGGGTCAGCA‑GGG‑CTTTGGTTTCCTGGGAGGGATACGGTTTGTTTGCAG‑AGGCGCCGCCCTTcgc > 2:154137/1‑150 (MQ=255) aaTCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTcgca < 2:942026/151‑1 (MQ=255) cacaAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGAATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTcgcaac < 2:430524/150‑1 (MQ=255) caAACGCGATATGGTACGGCGGGCAAGCCGCAGTACCGGGGGGACGCACTTTTTCGAAGAGGGAGGTTTTTA‑GTTTTCCGGGGGGCAGCA‑GGGCCTTTGGTTT‑CTGGTAGGGGTAAGTTTTGTTGGCAG‑GAGCGCCGCCTTTtgccacg‑c > 1:839451/1‑150 (MQ=255) aaaCGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTcgcaacg‑caa < 1:1006864/151‑1 (MQ=255) aaaCGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTcgcaacg‑caa < 2:747132/151‑1 (MQ=255) aaCGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTCGCAACG‑Caaa < 1:1216909/151‑1 (MQ=255) aCGCGATATGGTACGGCGGGCGGGGGGCAGTACCGAGGGGATGCATTTTTTTGATGAGGGAGATTTTTT‑GCTTGACCGGAGGAAGAT‑GGG‑CTTTGGGTTTCTGGTTGAAATAGGTTTTTTTTGTAG‑AAGCGCCGCCTTTctgaagg‑caa > 1:322858/1‑149 (MQ=255) atGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTCGCAACG‑CAAAGGAAttt < 1:189082/150‑1 (MQ=255) gTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTCGCAACG‑CAAAGGAATTTGTAc < 2:1141686/151‑1 (MQ=255) tACGGCGGGCAGGGCGGAGGACCGCGGGGACGCATTTTTTTGTCGAGGAGGTTTTTTA‑GTTTGCCCGGGGGCCGCG‑GGG‑CGTTGGTTTCCTGGTAGAGATACGTTTTGTTGGGAG‑AGCCGCCGCCTTTCGCCACGCCAAAGAAAttttaat > 1:114139/1‑147 (MQ=255) tACGGCGGGCAGGCTGCAGTACCGAGGGGGCGCATTTTTTCGTACAGGGAGGTTTTTA‑GTTTTCCGGGGGTCAGCA‑GGG‑GTTTTGGTTTCTGGGAGAGATACGTTTTTTTGGGGG‑AGCCGCCGCCCTTTCCCACG‑CAAAGGGATTTGttat > 1:78450/1‑148 (MQ=255) ggcggGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTCGCAACG‑CAAAGGAATTTGTACTCAt > 2:1131277/1‑151 (MQ=255) gCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTCGCAACG‑CAAAGGAATTTGTACTCATCGCCATCTAc < 2:1007956/149‑1 (MQ=255) gCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTCGCAACG‑CAAAGGAATTTGTACTCATCGCCATCTAc < 1:708236/149‑1 (MQ=255) aGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTCGCAACG‑CAAAGGAATTTGTACTCATCGCCATCTACcgcg < 2:721637/151‑1 (MQ=255) ccGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTCGCAACG‑CAAAGGAATTTGTACTCATCGCCATCTACCGCGTAc > 2:932390/1‑150 (MQ=255) gAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTCGCAACG‑CAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAgg > 1:58925/1‑151 (MQ=255) gAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTCGCAACG‑CAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAgg < 2:1052567/151‑1 (MQ=255) gAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTCGCAACG‑CAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAgg > 1:627875/1‑151 (MQ=255) gggTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTCGCAACG‑CAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTc < 2:9180/151‑1 (MQ=255) gggTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTCGCAACG‑CAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTc < 2:633284/151‑1 (MQ=255) tACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTCGCAACG‑CAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCGAt > 2:265975/1‑151 (MQ=255) | AACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCA‑GTTTGCCGGGAGTCAGCA‑GGG‑CTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAG‑AGCCGCCGCCTTTCGCAACG‑CAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCGAT > NC_000913/4346527‑4346825 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |