| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,478,324 | 0 | T | G | 41.1% | 110.4 / 10.9 | 56 | G33G (GGT→GGG) | ynbB | putative CDP‑diglyceride synthase |
| Reads supporting (aligned to +/- strand): ref base T (7/26); new base G (23/0); total (30/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.85e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTT‑GACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTT > NC_000913/1478176‑1478473 | aCTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGa < 2:924948/151‑1 (MQ=255) gCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAgg < 2:238702/150‑1 (MQ=255) cGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGtt < 1:580514/151‑1 (MQ=255) cGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTAc < 2:966331/150‑1 (MQ=255) gTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGt < 1:771865/151‑1 (MQ=255) gTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGt < 2:190210/151‑1 (MQ=255) tGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTa < 2:166639/150‑1 (MQ=255) gTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTATCCGGGA‑GGAAAAGGGGGGGGGGTACATTACGTAtt > 2:183506/1‑151 (MQ=255) cGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTc < 2:456000/150‑1 (MQ=255) cGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTc < 1:239764/150‑1 (MQ=255) cGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCg < 2:406761/151‑1 (MQ=255) cGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCg < 1:104571/151‑1 (MQ=255) gCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTATCGGGGG‑GGAAAGGGGGGGGGGTTTTATTCCGTATTCGTTcccg > 1:709539/1‑147 (MQ=255) tATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTgggg < 1:662766/149‑1 (MQ=255) cTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCGGGGA‑GAAAAGGGGGGGAGGGTACATTTCGTATTTGTACCTGGTGGGGGGTC‑GTCAtt > 1:53034/1‑151 (MQ=255) tGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGG‑GAAAAGGGGGGGGGGGTACATTACGTATTCGTACCTGGTGGGGGATC‑GTCAttt > 2:807105/1‑151 (MQ=255) tGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGGTAACGGAGG‑GAAAAGGGGGGGAGGGTATATTACGTATTCGTACCTGGTGGGGCATC‑GGCAttt > 1:68142/1‑151 (MQ=255) taATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAACGGGGA‑GAAAAGGGGGGGGGGGTTCATTACGTATTTGTAACTGGTGGGGGGTC‑GTCATttttttttt > 2:180486/1‑150 (MQ=255) aaTGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCCGGGA‑GAAAAAGGGGGGGGGGTACAATACGTATTTGTACCTTGGGGGGGATC‑GTCATTTGtttttt > 1:233904/1‑149 (MQ=255) aaTGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAACGGGGAGGAAAAGGGGGGGGGGTTAC‑TTTTGTATTTGTACATCGGGGGGGGTCGGTCttttttttttt > 1:827546/1‑150 (MQ=255) ggAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTg < 1:519162/151‑1 (MQ=255) aaaaaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGA‑GAAAAGGGGGGGGGGGTATATTACGTATTTGTACCTGGTGGGGGATC‑GTCATTTTTTTTTTAAt > 1:141224/1‑145 (MQ=255) ctctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTATCAGGGA‑GAAAAAGGGGGGGGGGTACATTACGTATTCGTACCTGGTGGGGCCTC‑GTCCTTTGtttttttattgggatt > 1:823870/1‑135 (MQ=255) acacTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCt < 1:464481/151‑1 (MQ=255) cacTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCt < 1:253295/150‑1 (MQ=255) cgcgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGAtgac < 1:140420/150‑1 (MQ=255) cgcgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGAtgac < 2:278589/150‑1 (MQ=255) aaTTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATgacgtt‑gacgtt < 2:587588/150‑1 (MQ=255) aaTTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAACGGGGA‑GAAAAGGGGGGGAGGGTATATTACGTATTTGTACCTGGGGGGGGATC‑GTCATTTTTTTTTTTATTGTGATTTTTGGTCCCCGCTGGGTGGCGTT‑GACGttt > 1:465037/1‑151 (MQ=255) ttAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGGTATCGGGGG‑GAAAACGGGGGGAGGGTTATTTTTGTATTCGGGTCTAGGGGGGCGGA‑GGTATTTTTTTTTTTATGGTGATTTTTTGTCCACGCTGGGTGGGGGT‑GTCGttttttt > 1:332690/1‑150 (MQ=255) tAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAACGGGGG‑GAAAAGGGGGGGAGGGTTCATTTCTTATTCTTACCTTGGGGGGGATC‑GTCATTTTTTTTTTTATGGTGATTTTTTGTCCCCCCTGGGTGACGTT‑GTCGtttttt > 1:488122/1‑149 (MQ=255) aGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGG‑GAAAAGGGGGGGGGGTTAGAATAAGTATTCGTAACTGGGGGGGGATG‑GTCATTTGTTTTTTTATGGTGATTTTAGGTCCACGCTGGGTGAGGTTGGAGGttttttc > 2:370052/1‑149 (MQ=255) aGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAGAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTT‑GACGTTTTTTgc < 2:854837/150‑1 (MQ=255) aaCGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCCGAGG‑GAAAAAGGGGGGAGGGTACATTACGTATTCGTACCTGGTGGGGCACC‑GTCATTTTTTTTTTTATGGGGGTTTTTGGTCCACGCCGGGTGGCGTT‑GAAGGTTTTTgtggt > 2:604478/1‑146 (MQ=255) cGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGGGGGGGTACATTACGTATTTGTACCTGGTGGGGGATC‑GTCATTTGTTTTTTTATGGGGATTTTAGGGCCACCCTGGGTGACGGT‑GACGTTTTTTTCGCTGAt > 2:698877/1‑151 (MQ=255) gCTGATAAACCGTTTTCTGCTATGGCGGTTTCCCGGGA‑GGAAAAGGGGGGAGGGTACATTACGTATTTGTAACTGGGGGGGGCTC‑GCCCTTTGTTTTTTTATGGTGATTTCAGGTCCACGCCGGGTGACGTT‑GTCGTTTTTTtcg > 2:252806/1‑145 (MQ=255) gCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTT‑GACGTTTTTTGCGCTGATa < 1:333029/151‑1 (MQ=255) tGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGG‑GAAAAGGGGGGGAGGGTTCATTACGTATTTGGACCTGGGGGGGGATC‑GTCATTTGTTTTTTAATGGGGGTTTTAGGGCCACGCTGGGTGACGTT‑GTCGtttttt > 1:27144/1‑140 (MQ=255) aTAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTT‑GACGTTTTTTGCGCTGATAAGtt < 2:474785/151‑1 (MQ=255) aTAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGGGAGGGTACATTTCGTATTCGTACCTGGTGGGGGATC‑GTCATTTTTTTTTTTATGGGGAATTTACGTCCACCCTGGGTGACGTT‑TACGTTTTTTTCGCTGATaa > 2:809726/1‑148 (MQ=255) aaCCGTTTTCTGCTATGGCGGTTACCGGGGG‑GAAAAAGGGGGGAGGGTACATTACGTATTCGTACCTGGGGGGGGCTC‑GTCATTTTTTTTTTAATGGTGATTTTAGGGCCACGCTGGAGGACGTT‑GACGGTTTTTTCGCTGGTAAAttttt > 1:573910/1‑150 (MQ=255) gTTTTCTGCTATGGCGGTTACCGGGGG‑GGAAAGGGGGGGGGGGTTCATTTCGTTTTCGTACCTGGTGGGGGATC‑GGCATTTGTTTTTTTATGGGGATTTTAGGGCCACGCTGGGTGACGTT‑GACGTTTTTTTTGGTGGTAAGtttttt > 1:689809/1‑146 (MQ=255) gTTTTCTGCTATGGCGGTTACCGGAGA‑GGAAAGGGGGGGGGGGTTCATTTCCTATTTGTTCCTCGTGGGGCAGC‑GTCATTTGTTTTTTTATTGGGGTTTCTGGTCCACGCTGGGTGGCGTT‑GTCGTTTTTTTCGCTGGTTAGtttttt > 1:156098/1‑146 (MQ=255) tttcttagaTGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTT‑GACGTTTTTTGCGCTGATAAGTTTTCTGGCa < 1:862607/142‑1 (MQ=255) gCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTT‑GACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGa < 2:899311/149‑1 (MQ=255) cTATGGCGGTTACCGGGGA‑GAAAAGGGGGGGGGGTTACATTACGTATTCGTACCTGGGGGGGGGTC‑GTCATTTGTTTTTTTATGGTGATTTCAGGGCCACGCTGGGTGACGTT‑GGGGGTTTTTTCGCTGATAAGTTTTTTGGCATTGaaa > 1:729028/1‑150 (MQ=255) tGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGGTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAAAGGGGATTTCAGGGCCCCGCTGGGTGACGTT‑GTCGTTTTTTTTGTTGATAAGTTTTCTGGCATTGAAAGaa > 1:54756/1‑150 (MQ=255) cGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTT‑GACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATAtt > 1:813435/1‑151 (MQ=255) gTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTT‑GACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGt > 2:261475/1‑151 (MQ=255) aCCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTT‑GACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTAc < 2:252304/150‑1 (MQ=255) gaga‑gaAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTT‑GACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCtt > 1:939334/1‑150 (MQ=255) gaga‑gaAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTT‑GACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTta < 2:483014/151‑1 (MQ=255) aaaaGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTT‑GACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCt > 1:569015/1‑151 (MQ=255) aaaGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTT‑GACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTg > 2:685543/1‑151 (MQ=255) aGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTT‑GACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGt > 1:63266/1‑150 (MQ=255) gTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTT‑GACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACAttt < 1:183504/151‑1 (MQ=255) | ACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATC‑GTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTT‑GACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTT > NC_000913/1478176‑1478473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |