| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,998,178 | 0 | G | A | 40.4% | 61.8 / 26.1 | 47 | A66T (GCG→ACG) | uvrD | DNA‑dependent ATPase I and helicase II |
| Reads supporting (aligned to +/- strand): ref base G (2/26); new base A (19/0); total (21/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.01e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCT > NC_000913/3998041‑3998323 | cgcCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGAt < 2:820510/151‑1 (MQ=255) cACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCg < 1:678082/151‑1 (MQ=255) cagcaACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGGGGAGTTTTACAAAAAAAAGGCGGCGGGGGTGCGTcc > 2:101042/1‑149 (MQ=255) agcaACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATc < 1:266317/151‑1 (MQ=255) ggcgggcgCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑g < 1:487594/151‑1 (MQ=255) gggcgcgggcAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGc < 1:578758/151‑1 (MQ=255) gggcgcgggcAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGc < 1:473206/151‑1 (MQ=255) gcgcgggcAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCAc < 1:581883/151‑1 (MQ=255) cgcgggcAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCACg < 1:929060/151‑1 (MQ=255) gggcAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGGCGTTTACCCACAAAACGGCGGCGGGGGGGCGTCATCGTATCGGGCGAATGAT‑ggg > 1:390081/1‑144 (MQ=255) aGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCACGAGCCAgg < 1:729691/151‑1 (MQ=255) tGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGGCGGTTTCCAACAAAACGGGGGCGGGGAAGGGGCACCGTATACGGCAACCGGA‑GGGCACGAGcc > 2:264679/1‑146 (MQ=255) aGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACCTTTTCTCAAAAAACGGCGGCGGGGATGCGTCCTCGGCTCGGGGAACAGAT‑GGGCCCGAGcccaggggg > 2:940861/1‑141 (MQ=255) gcgTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGGTTTCCAACAAAACGGCGGGGGGGGGGGGTCATCGTATCCGGGGACAGAT‑GGGGACCGGCCAGGGGGGGGTGTggg > 2:118728/1‑150 (MQ=255) gTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCACGAGCCAGGGCGGTATGTGGGTCg < 1:852609/151‑1 (MQ=255) gTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGAAGTTTTTCAACAAAACGGCGGCGGGGGAGCGGCCTCGTATTGGGGGAATGAT‑GGGGACGAGCCAGGGGGGGGTGTggg > 2:397336/1‑148 (MQ=255) tGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTTTCAACAAAACGGCGGCGGGGAGGGGTCATCGTATCTGGGAACTGAT‑GGGGACGAGCCAGGGGGGGGTGTGGGGCGGGAccct > 2:856894/1‑149 (MQ=255) gCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTc < 2:65878/151‑1 (MQ=255) gCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGGTTTCCAACAAAACACGGGCGGGGGTGCGGCCTCATCTCTGGCCACCGAT‑GGGCGCGAGCCCGGGGGGGTTGTGGGGCggggccct > 1:369993/1‑144 (MQ=255) tCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGGTTACCAAAAAAACGGGGGCGGGGATGCGTCATCGGATTGGGGGACCGGT‑GGGGACGAGCCCGGGGGGGGTGTGGGGGGGGACCCTCCAc > 2:866691/1‑151 (MQ=255) cGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACg < 2:463912/151‑1 (MQ=255) gTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACgg < 1:383968/151‑1 (MQ=255) gCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGGCGTTTTCCCAAAAAACGGCGGCGGGGAAGCGTCCTCGTATCGGGGAACTGAT‑TGGCCCGAGCCAGGGCGGGATGTGGGTCGGCCCCCTCCCCCGGCTg > 2:17673/1‑150 (MQ=255) gCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTTTCAACAAAAAGGCGGCGGGGAGGCGGCCTCGTATCGGGGGACTGCT‑GGGGCCCAGCCACGGGGGGGTGGGGGGGGGGACCCTCCACcgg > 1:433349/1‑147 (MQ=255) tgaGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGGTTTCCCAAAAAACGGCGGGGGGGGTGCGTCATCGTATCGGGGAACTGAT‑GGGCACGAGCCAGGGCGGGGTGTGGGGCGGGGCCCTCCACGGGCCGGCGCCCCGttt > 2:709121/1‑151 (MQ=255) gaGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGttt < 2:50923/150‑1 (MQ=255) gaGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTtg < 1:821268/151‑1 (MQ=255) gTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTtgctgc < 1:197087/151‑1 (MQ=255) aaaaCTGCTCGCCATACTCGATTATGGCGGTGACAATTTTCAAAAAAACCGAGGCGGGAATGAGTCATCGGAACGGGGGACTGAT‑GGGGGCGAGCCAGGGGGGGGTGTGGGGGGGGCCCCCCCACGGGCCGGGGCCCCGGTtgct > 2:82335/1‑145 (MQ=255) aaaCTGCTCGCCATACTCGATTATGGCGGTGACGGTTACCCACAAAAACGCGGCGGGGGTGCGTCATCCTATCGGGCCACTGAT‑GGGGGCGAGCCCGGGGGGGATGTGGGGGGGCCCCCTCCACCGGCggggggccccctt > 1:235741/1‑131 (MQ=255) aaCTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTgcgc < 1:715761/151‑1 (MQ=255) aaCTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAAACGCGGCGGGGATGCGGCATCCTATCGGGCAACTGAA‑GGGCACGAGGCACGGCGGGGTGGGGGGGGGGGCCCTCCCCCGGCCGGCGCACCGCTTGCttcg > 1:231978/1‑146 (MQ=255) cTCGCCATACTCGATTATGGCGGTGAAGTTTACCAACAAAAACGCGGCGGGGGTGTGTCGTCGGTTCGGGCAAATGATGGGGCACG‑GCCAGGGGGGGGTGTGGGGGGGCCCCCCCCCCCGGCGGGGGCCCCGTTTTCTGCGGGCGCGccc > 1:673333/1‑149 (MQ=255) cGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCTCGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCata < 1:322919/150‑1 (MQ=255) gCCATACTCGATTATGGCGGTGACGTTTTCCCACAAAACGGCGGCGGGGATGCGTCATCGTATCGGGGAACAGAT‑GGGGACGAGCCAGGGGGGGGTGTGGGGGGGCACCCTCCCCCGGCTGGCGCCCCCGTTTCTGCGTGGGcccc > 2:830310/1‑146 (MQ=255) aTACTCGATTATGGCGGTGACGGTTTCCAACAAAACCGCGGCGGGGGTGCGTCATCCTATCGGGGAAATGAT‑GGGGCCGAGCCCGGGGGGGGTGGGGGGGGGGCCCCTCCCCCGGCCGGGGCACCCGTTTCTGCGTGCGCCCCctattg > 1:149832/1‑147 (MQ=255) aTACTCGATTATGGCGGGGACGGTTTTCAACAAAACGGCGGCGGAGATGCGTCATCCTGTCGGGCAAAAGAT‑GGGGACGCACCAGGGGGGGATGTGGGGCGGCACCCTCCCCCGGGGGGCGGCCCGCTTGCTGCGTGCGCAccccatag > 1:740745/1‑143 (MQ=255) aCTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACg < 1:843125/150‑1 (MQ=255) aCTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACg < 1:720665/150‑1 (MQ=255) cGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGcc < 2:833406/149‑1 (MQ=255) cGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGACAACTGAT‑GGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCa < 1:263399/150‑1 (MQ=255) ggCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCGCGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGcc < 1:302735/150‑1 (MQ=255) aCGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGAtt < 1:781022/151‑1 (MQ=255) cGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGAttt < 1:642244/151‑1 (MQ=255) aacaaAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATcc > 1:697434/1‑151 (MQ=255) aacaaAGCGGCGGCGGAGATGCGTCATCATATCGGGCAACTGAT‑GGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATcc < 1:759392/151‑1 (MQ=255) acaaAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCt > 1:632078/1‑151 (MQ=255) | CGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGAT‑GGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCT > NC_000913/3998041‑3998323 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |