Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	535,918	0	A	G	40.7%	93.4 / 11.1	60	E70G (GAG→GGG)	hyi	hydroxypyruvate isomerase
Reads supporting (aligned to +/- strand):  ref base A (9/26);  new base G (24/0);  total (34/26)
Fisher's exact test for biased strand distribution p-value = 2.15e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACG  >  NC_000913/535768‑536067                                                                                                                                                        |                                                                                                                                                      gTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGa                                                                                                                                                       <  1:265752/151‑1 (MQ=255)              tGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGCGGTCTTGGTGGGGGGGGGGGGCgggcttgt                                                                                                                                           >  1:678148/1‑144 (MQ=255)               gcgcAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGc                                                                                                                                           <  1:231955/149‑1 (MQ=255)                cgcAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTg                                                                                                                                        <  2:885874/151‑1 (MQ=255)                cgcAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTg                                                                                                                                        <  1:341067/151‑1 (MQ=255)                  cAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGGGGCTGGGGGGGGGGGGGGCGCGGGATTGCCTGt                                                                                                                                       >  2:18172/1‑150 (MQ=255)                  cAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGGGAACGGGCGGCGGGGGGGCGCGGGATTGCCt                                                                                                                                         >  2:214413/1‑148 (MQ=255)                      gtgGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGGGACTGGGGGGGGGGGGGGGGCGGGATTGCCCGTAtt                                                                                                                                    >  2:267873/1‑149 (MQ=255)                         gTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTgg                                                                                                                               <  1:163047/151‑1 (MQ=255)                                cggcgTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGGGGGTGGGGGGCGGGGGGGGGCGGGATTTTCTGGTTTCCTgggc                                                                                                                             >  1:506298/1‑144 (MQ=255)                                   cgTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTgaagaa                                                                                                                     <  2:568152/151‑1 (MQ=255)                                       gAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGt                                                                                                                 <  2:624307/151‑1 (MQ=255)                                                 ttCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATg                                                                                                        <  1:972407/150‑1 (MQ=255)                                                 ttCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATg                                                                                                        <  1:617955/150‑1 (MQ=255)                                                 ttCCTTATGACTACGACATTGAAGAATTAAAACATGAGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGCGGGGGGTCTGGG‑GGGGGGGGAGCGGGGGATTACGCATTTTCTTGGCCCTGGAGAAGAGGTTCggggggg                                                                                                       >  2:509923/1‑147 (MQ=255)                                                             aCGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTagcagcagc                                                                                           <  1:606708/151‑1 (MQ=255)                                                             aCGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGGGGCTGGGGGGCGGGGGGGCGCGGGATTTCCTGGATTTCTGGCCGGGGAGAAGAGGTTTGGGGTGGGGGagcag                                                                                               >  2:221099/1‑147 (MQ=255)                                                              cGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGACTAGGCGGGGGGGGGGCGCCGGATTGCCCGGATTCCCGGTCGTGAAGAAGGGGTTCggggtggggtgggcacagc                                                                                           >  2:755917/1‑141 (MQ=255)                                                                      aagaaTTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGGGAACGGGCGGGGGGGGGGCGCGGGGTTGCCTGTATTTCTGGCCCGGGAGAAGAGGGTCCGGGTGGCGGAGCAGACGCGGTTCGt                                                                                    >  1:858104/1‑149 (MQ=255)                                                                       agaaTTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTAt                                                                                 <  1:11703/151‑1 (MQ=255)                                                                          aTTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGGGAGTGGGACGGGGGGGGGGGCGGCATTGTCTGTTTTTCTTGCCGTGGAGAAAAAATTTGGGAGGGGGTAGCCGCAGACATTCtttttttc                                                                              >  2:334973/1‑146 (MQ=255)                                                                            tAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGGGCGCGGGATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTTGGGGTGGCGGAGCAGCAGCGATTCGTTATGccc                                                                             >  2:407630/1‑150 (MQ=255)                                                                            tAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGGGGCTGGGCGGCGGGGGGGGGCGGGATTGCCTGTATTTCTGGCCGTGAAGAAGAGGTTCGGGGTGGCGTAGCAGCAGCGATTTGTTATGccc                                                                             >  2:495597/1‑150 (MQ=255)                                                                                 cATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGGGGCTGGGCGGCGGGGGGGCGCGGGATTGCCTGTATTTCTGGCCGTGAAAAAGAGGTTTGGGGGGGCGTGGGAGCAGCGATTTGtttttgcc                                                                             >  1:518294/1‑139 (MQ=255)                                                                                      gCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTggg                                                                   <  2:619947/150‑1 (MQ=255)                                                                                      gCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGGGGCTGGGGGGCGGGGGGGCGCGGGATTGCCTGTATTTCTTGGCGTGAAGAAGAGGTTTGGGGGGGGGTAGGCGCAGCGGTTTGTTTTTCCCCTGCGCTggg                                                                   >  1:614366/1‑150 (MQ=255)                                                                                      gCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGGGACTGGGCGGCGGGGGGGCGCGGGATTGCCTGTATTTCTGGCCGGGGAGAAGAGTTTTGGGGTGGGGGAGCAGCAGCGGTTTGTTATGCCCGTGCGCTggg                                                                   >  2:390732/1‑150 (MQ=255)                                                                                            gAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATaaa                                                            <  1:148107/151‑1 (MQ=255)                                                                                              gTAATAAACTCGATCATACGCTGCACAATTTACCGGC‑GGGGGGATGGGCGGCGGGGGGGGGCGGGATTGCCTTTATTCCTGGCCGGGGAGAAGAGGTTCGGGATGGGGGAGCAGCAGCGGTTTGTTATGTCCCTGCGCTGGGGAaaaaaa                                                           >  1:891924/1‑150 (MQ=255)                                                                                               taataaACTCGAACATACGCTGCACAATTTACCGGC‑GGGGGACTGGGGGGCGGGGGGGGGCGGGATTGCCTTTATTTCTTGGCGGGGAGAAGAGGTTTGGGGTGGGGTGGCAGCAGCGATTCGTTATGGCCGCGCGCTGGGGGATaaaaa                                                          >  1:298342/1‑150 (MQ=255)                                                                                                aataaACTCGAACATACGCTGCACAATTTACCGGC‑GGGGGGATGGGCGGCGGGGGGGGGCGGGATTGCCTGTATTTCCGGCCGGGAAGAAGAGATTTGGGGTGGCGGAGCAGCAGCGCCTCGGGTTGCCCCGGCGCTGGGGTATaaaaaa                                                         >  1:905936/1‑150 (MQ=255)                                                                                                aataaACTCGAACATACGCTGCACAATTTACCGGC‑GGGGGACTTGGGGGCGGGGGGGCGCGGGATTGCCTGTATTTCTGGCCGGGAAGAAGAGGTTTGGGGGGGGGTAGCAGCAGCGATTCGTTTTTCCCGTGCGCTGGGGAATaaaaaa                                                         >  1:476800/1‑150 (MQ=255)                                                                                                 ataaACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGGCTGGGCGGGGGGGGGGCGCGGAAATTTCTCTACTCCTGGCCGGGGAGTAGAGCTTCGGGGCGGGGTAGCAGCCGCGGGTTGCTATTTCCGTGCGGCGGGGAATtaaaaa                                                         >  2:147217/1‑149 (MQ=255)                                                                                                 ataaACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGGGGGGGAGCGCGGGATTGCCTGTATTTCTGGCCGTGGAGGAGAGGTTCGGGGTGGCGGAGCAGCAGCGATTCGTTTTGCCCGCGCGCTGGGTGATaaaaaaa                                                        >  2:405121/1‑150 (MQ=255)                                                                                                 ataaACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGAACGGGGGGGGGGGGGGGGCGGTATTTTCTGTATTTACGGGCGTGAAGAAAGGCTTTGGGGTGGGGAAAAAGCAGCGGTTCCGTATGACCGAGCGCTGGGGAATaaaaaaa                                                        >  1:747366/1‑150 (MQ=255)                                                                                                   aaaCTCGAACCTACGCTGCACAATTTACCGGC‑GGGTGACGTGGGGGGGGGGGGGAGCGGGGTTTCCTGTATTTCCCGGCGGGAAGAAAAGGTTTGGGGGGGGGGAGCAGCAGAGTTTTGGTATTCCCCTGCGCGGTGGCATAAAAAAAtt                                                      >  1:791876/1‑150 (MQ=255)                                                                                                    aaCTCGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGGGGCGGGGGAGCGCGGTATTGCCTGTATTTCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGGAATAAAAAAATTa                                                     >  2:768708/1‑150 (MQ=255)                                                                                                       ttgAACATACGCTGCACAATTTACCGGC‑GGGGGACTGGGGGGCGGGGGGGGGCGGGATTGCCTGTATTTCTGGCCGTGAAGAAGAGGTTTGGGGTGGGGGAGGAGGAGCGATTCGTTTTTTCCCTGCGCTTGGTAATaaaaaaa                                                        >  2:554419/3‑144 (MQ=255)                                                                                                        cGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACt                                                  <  1:534869/149‑1 (MQ=255)                                                                                                        cGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTg                                                 <  1:540262/150‑1 (MQ=255)                                                                                                        cGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGt                                                <  2:865187/151‑1 (MQ=255)                                                                                                        cGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGt                                                <  2:748613/151‑1 (MQ=255)                                                                                                          aaCATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCt                                              <  2:13331/151‑1 (MQ=255)                                                                                                          aaCATACGCTGCACAATTTACCGGC‑GGGTGAATTGGGGGGGGGGGAGCGCGGGATTGGCCGTATTTTTGGTCGTCAGGAAGAGGTTTTGGGTGGCGTAGCAGAAGCGAGTCGCTTTGGCCGTGCGCTGGGTTATaaaaaaaatta                                                    >  2:790634/1‑141 (MQ=255)                                                                                                          aaCATACGCTGCACAATTTACCGGC‑GGGGGACTGGGGGGGGGGGGAGCGGGGGATTGCCTGTATTTCTGGCCGTGGAGAAGAGGTTTGGGGTGGCGTAGCAGCAGCGGTTTGGTATGTCCGCGCGCTGGGGAATAAAAAAATTAAATGTCt                                              >  1:461313/1‑151 (MQ=255)                                                                                                           aCATACGCTGCACAATTTACCGGC‑GGGTGACGGGGCGGCGGGGGCGCGCGGTACTGCCCTGATTCCTGGCCCTGAAGGGGAGTTTCGGGATGGGCTTGCAGAAGCGATTCCTTATGCCCCTGACCTTGGTAATCAAAAAATTAAATg                                                 >  1:622238/1‑147 (MQ=255)                                                                                                           aCATACGCTGCACAATTTACCGGC‑GGGGGACTGGGCGGCGGGGGGGCGCGGGATTGCCTGTATTCCTGGCCGTGAAGAAGAGGTTCGGGGTGGCGGAGCAGCAGCGATTCGTTATGCCCCTGCGCTGGGGAATAAAAAAATTAACTGTCt                                              >  2:694959/1‑150 (MQ=255)                                                                                                             aTACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGt                                           <  2:442995/151‑1 (MQ=255)                                                                                                               aCGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCg                                         <  1:360079/151‑1 (MQ=255)                                                                                                                        aaTTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGc                                <  2:13115/151‑1 (MQ=255)                                                                                                                         aTTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGcc                               <  2:885611/151‑1 (MQ=255)                                                                                                                            tACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCgg                             <  1:431375/150‑1 (MQ=255)                                                                                                                             aCCGGC‑GGGTGACTGGGCGGGGGGGGGGCGCGGGATTGCCTGTATTCCTGGCCGTGGAAAAGAGGTTCGGGGTGGGGGAGCAGCAGCGGTTCGTTTTGCCCGCGCGCCGGGGAATAAAAAAAATAACTGTCTTGTCGGTAAAAAGcc                               >  1:383426/1‑147 (MQ=255)                                                                                                                              ccGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTg                          <  2:45368/151‑1 (MQ=255)                                                                                                                                ggc‑ggGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTgg                         >  1:965194/1‑150 (MQ=255)                                                                                                                                        gACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTcagca                 <  1:430418/151‑1 (MQ=255)                                                                                                                                           tGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTg              >  2:65360/1‑151 (MQ=255)                                                                                                                                             ggcggcGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGTTGGGGAATAAAAAAATTAACTGTCTGGTCGGTAAACCGCCGGCTGGTTTCAGCAGGGa             >  1:672645/1‑150 (MQ=255)                                                                                                                                                  cggGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAAc           >  1:148568/1‑147 (MQ=255)                                                                                                                                                  cggGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGAt       <  1:878923/151‑1 (MQ=255)                                                                                                                                                       gAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACg  <  2:634498/151‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                      GTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAACATGTGCTGGCGAGTAATAAACTCGAACATACGCTGCACAATTTACCGGC‑GGGTGACTGGGCGGCGGGGGAGCGCGGTATTGCCTGTATTCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACG  >  NC_000913/535768‑536067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑