BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	40,515	0	G	T	47.5%	132.3 / 71.5	121	Q473K (CAA→AAA)	caiT	putative transporter
Reads supporting (aligned to +/- strand): ref base G (16/46); new base T (56/0); total (75/46)
Fisher's exact test for biased strand distribution p-value = 2.03e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 125 total aligned reads displayed.

ATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAAGAGCAGGCGTTAAC  >  NC_000913/40366‑40665

atcatTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGa                                                                                                                                                            <  1:284486/151‑1 (MQ=255)

  catTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGaa                                                                                                                                                           <  2:858380/150‑1 (MQ=255)

   atTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTg                                                                                                                                                             >  1:219300/1‑147 (MQ=255)

    tttAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑GTTGAAACg                                                                                                                                                        >  1:1065734/1‑151 (MQ=255)

     ttaaattaaaATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATCgg                                                                                                                                                       >  2:206432/1‑151 (MQ=255)

      taaattaaaATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATCgg                                                                                                                                                       >  2:648859/1‑150 (MQ=255)

      taaattaaaATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGGAtacg                                                                                                                                                       >  1:128915/1‑147 (MQ=255)

        aattaaaATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGGACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGttt                                                                                                                                                    <  1:878024/151‑1 (MQ=255)

           taaaATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATCGGgtttcg                                                                                                                                                 >  2:251731/1‑148 (MQ=255)

              aaTCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATAGGGTTCAGgcc                                                                                                                                              >  1:920675/1‑151 (MQ=255)

               aTCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTGATTGGCTTCAGgcc                                                                                                                                              >  1:558240/1‑150 (MQ=255)

               aTCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGgccg                                                                                                                                             <  2:823943/151‑1 (MQ=255)

                 ccATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑CTTAAAAGGGTTTTAGtccgcc                                                                                                                                           >  2:566799/1‑151 (MQ=255)

                   aTCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATCGGTTTTAgggc                                                                                                                                              >  1:313030/1‑144 (MQ=255)

                   aTCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATCGGGTTTAGgccgcc                                                                                                                                           >  1:272644/1‑149 (MQ=255)

                   aTCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATCGGGTTTAGgccgcc                                                                                                                                           >  1:265948/1‑149 (MQ=255)

                    tCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTTATCGGGTTCAGggcgcc                                                                                                                                           >  1:172515/1‑148 (MQ=255)

                      tttCAACCTCTTGATATTTTAGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTGATCGGTTTTAGgccgcc                                                                                                                                           >  1:1012191/1‑146 (MQ=255)

                       ttCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGa                                                                                                                                         <  2:930972/147‑1 (MQ=255)

                          aaCCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTTATTGGGTTTAGGGCGCCGC‑GCGcc                                                                                                                                   >  1:618164/1‑149 (MQ=255)

                          aaCCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTTATTGGGTTTAGGCCGCCGG‑GCGcc                                                                                                                                   >  1:410862/1‑149 (MQ=255)

                          aaCCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATTGGGTTCAAGCCGCCGG‑GCGcc                                                                                                                                   >  2:810188/1‑149 (MQ=255)

                           aCCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATCGGTTTCAGGCCGCCGC‑GCGcc                                                                                                                                   >  1:1006800/1‑148 (MQ=255)

                            ccTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTTATCGGTTTCAGGCCGCCCA‑GCGcc                                                                                                                                   >  1:683567/1‑147 (MQ=255)

                               ctTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAAACGGTTTCAGGCCCCCCC‑GCGCccgc                                                                                                                                >  2:175778/1‑147 (MQ=255)

                                 tGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTTATCGGGTTCTGGCCGCCGC‑GCGCCCGCCGaa                                                                                                                            >  1:811777/1‑149 (MQ=255)

                                  gATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATCGGGTTTAGGGCGCCGA‑GCGGCCGCAGaa                                                                                                                            >  2:164983/1‑148 (MQ=255)

                                  gATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGTATCGGGTTTAAGGCGCCCG‑GCGCCCGCAGaaaa                                                                                                                          >  2:934899/1‑148 (MQ=255)

                                   atatTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTGATCGGTTTCAGGGCGCCGC‑GCGCCCGCCGAACaa                                                                                                                         >  1:307676/1‑150 (MQ=255)

                                   atatTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATAGGGTTTAGggcgccccgcccccgccaaa                                                                                                                             >  1:680173/1‑133 (MQ=255)

                                   atatTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGTATCGGGTTTAGGCCGCCGC‑GCGCCAGCAGAaaaa                                                                                                                         >  1:590730/1‑150 (MQ=255)

                                      ttttGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTTAAAGGGTTTAGGGCGCCCG‑GGGCCCGCGGAACaaaa                                                                                                                       >  1:633690/1‑147 (MQ=255)

                                      ttttGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATTGGTTTTAGGCCGCCGC‑GCGCCCGCCGAaaaa                                                                                                                         >  1:860232/1‑147 (MQ=255)

                                      ttttGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATCGGGTTCAGGCCGCCCG‑GCGCCCGCAGAACaaaa                                                                                                                       >  2:1032286/1‑147 (MQ=255)

                                      ttttGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAgg                                                                                                                                                <  2:842408/125‑1 (MQ=255)

                                       tttGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGGTTTAGGGCGCCGC‑GCGCCAGCAGAAAAATaac                                                                                                                     >  2:1057923/1‑148 (MQ=255)

                                         tGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGa                                                                                                                  <  2:24149/150‑1 (MQ=255)

                                            gggTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑t                                                                                                            <  2:1162182/151‑1 (MQ=255)

                                               ttaattaaTCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGGTTTTTATTGGGTTTCGGGCCCCCG‑‑GCGCCCGCCGAACAAAA‑CCCAT‑AA‑AGcc                                                                                                         >  2:292514/1‑151 (MQ=255)

                                               ttaattaaTCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATCAGGTTTAGGGCGCCGC‑GCGGCAGCACAAAAAAA‑ACGAT‑AA‑AGcc                                                                                                         >  1:1038422/1‑151 (MQ=255)

                                                taattaatCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑GTTTAATAGGGTTTAGGCCGCCGA‑GCGCCAGCAGAACAATA‑ACGGT‑AA‑TGcc                                                                                                         >  2:392404/1‑150 (MQ=255)

                                                 aattaatCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATTGGGTTTAAGCCCCCGA‑GCGCCCGCAAAACAAAA‑ACGAT‑AA‑Tgac                                                                                                         >  2:280555/1‑147 (MQ=255)

                                                 aattaatCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATTGGTTTTTGGGCGCCGAGGGGCCAG‑AGAAAAACA‑ACGAT‑AA‑TTcc                                                                                                         >  1:87110/1‑149 (MQ=255)

                                                 aattaatCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCa                                                                                                        <  1:950232/150‑1 (MQ=255)

                                                  attaatCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAAc                                                                                                      <  1:308078/151‑1 (MQ=255)

                                                   ttaatCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTTATCGGGTTTAGGCCGCCGC‑GCGCCCGCCGAACAAAA‑ACCCT‑AA‑TGccca                                                                                                       >  1:776604/1‑147 (MQ=255)

                                                       tCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑ATTTTATCGGGTTTCGGCAGCCGA‑GCGCCCGCACGACAATA‑AAGGT‑AA‑TACCAAcc                                                                                                     >  2:233095/1‑147 (MQ=255)

                                                        cTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTTATTGGTTTTTGGCCGCCGG‑GCGCCCGCAGAAAAAAA‑CCGAT‑AA‑ATGCAACCAG‑a                                                                                                 >  1:1114600/1‑149 (MQ=255)

                                                        cTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGGTTTAGGCCGCCGG‑GCGCCCGCAGAAAAAAA‑ACCAT‑AA‑AGCCaaac                                                                                                     >  1:953033/1‑144 (MQ=255)

                                                         tttCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTTATTGGTTTTAGGCCGCCGA‑GCGCCAGCAAAAAAAAA‑ACCAT‑AA‑TGACCACCAG‑aaa                                                                                               >  1:1171750/1‑149 (MQ=255)

                                                          ttCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AAt                                                                                               <  2:729968/149‑1 (MQ=255)

                                                            ccAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGa                                                                                            <  2:540853/150‑1 (MQ=255)

                                                             cAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGGTTTAGGCCGCCGG‑GCGCCAGCAGAAAAAAA‑ACCAT‑AA‑TACCAACCAG‑AATTGAcc                                                                                          >  1:660848/1‑151 (MQ=255)

                                                              aGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCa                                                                                         <  1:1100879/151‑1 (MQ=255)

                                                                ttCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTGATCGGGTTTAGGGCGGCCG‑GCGGCAGGAGAAAAAAA‑ACGCT‑AA‑TGCCAACCAG‑aaaataccca                                                                                        >  1:1168319/1‑142 (MQ=255)

                                                                  cTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATCGGGTTTAGGCCGCCCA‑GCGCCAGCAGAACAAAA‑ACCAT‑AA‑TGCCAAACAG‑AAATGACCaaac                                                                                      >  1:1037565/1‑148 (MQ=255)

                                                                    gTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAAACGGTTTTAAGCCGCCGC‑GCGCCAGCAGAAAAAAA‑ACGAT‑AA‑AGTCCACCCA‑AATTGACCAAc                                                                                       >  2:1018422/1‑147 (MQ=255)

                                                                     tttCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATCGGGTTTAGGCCCCCGA‑GCGCCCGCAGAACAAAA‑ACGAT‑AA‑TGGCAACCAA‑AAATGACCaa                                                                                        >  1:457550/1‑145 (MQ=255)

                                                                        cgcgTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTTATCGGGTTTAGGGCGCCGA‑GCGCCCGCCGAACAAAA‑CCGAT‑AA‑TGCCAACCAG‑AAATTACCCACCGATAcac                                                                               >  2:206094/1‑149 (MQ=255)

                                                                        cgcgTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATCGGGTTTAGGCCGCCGG‑GCGGCCGCAGAACAAAA‑ACGAA‑AA‑TGCCAACCAG‑AAATGAACAACCGATa                                                                                  >  2:1138354/1‑148 (MQ=255)

                                                                        cgcgTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGGTTTAGGCCGCCGC‑GCGCCCGCAGAAAAATA‑ACGAT‑AA‑TGCCCACCAA‑AATTTACCAAACCATa                                                                                  >  2:367538/1‑148 (MQ=255)

                                                                          cgTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATCGGGTTTAGGCCGCCGG‑GGGCCCGCAGAAAAAAA‑ACGGA‑AA‑TACCCAACAC‑AAATTACCAACCGaaa                                                                                  >  1:928326/1‑144 (MQ=255)

                                                                             cTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATAGGGTTTAGGCCGCCGA‑GCGCCCGCAGAAAAAAA‑ACCAT‑AA‑TGCCAAACAG‑AATTTACCAACCGAAAAGCAcc                                                                            >  1:980266/1‑149 (MQ=255)

                                                                               ttAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACcag                                                                          <  1:1068974/149‑1 (MQ=255)

                                                                               ttAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACca                                                                           <  2:962814/148‑1 (MQ=255)

                                                                                  ataaAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATTGGTTTTAGGCCGCCCA‑GCGCCCGCAGAACAAAA‑ACGAT‑AA‑TTCCAACCAG‑AAATTACCAACCGATACGcccc                                                                            >  2:1145451/1‑144 (MQ=255)

                                                                                  ataaAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCagag                                                                     <  2:514122/151‑1 (MQ=255)

                                                                                   taaAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTTATCGGGTTTCGGCCGCCGA‑GCGCCAGCAGAACAAAA‑CCGAA‑AA‑TGCCAACCAG‑AAATTACCAACCCATACCCAcc                                                                            >  1:563173/1‑143 (MQ=255)

                                                                                     aaGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCAGAggtg                                                                  <  2:857432/151‑1 (MQ=255)

                                                                                          gagCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATCGGTTTTAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCCATACGCACCCGCAGAGGGGGgtct                                                             >  2:856155/1‑151 (MQ=255)

                                                                                          gagCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCAGAGGTGGttct                                                             <  1:312475/151‑1 (MQ=255)

                                                                                          gagCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑ATTTAATAGGGTTTAGGGCCCCGA‑GGGCCCAGAGAAAAAAA‑TCCCT‑AA‑TTTCAACCAGAAATTTACCAACC‑AAACCCACCACCAAAggtgggt                                                               >  2:461630/1‑147 (MQ=255)

                                                                                                cACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTTAACGGGTTTAGGCCGCCCC‑GCGCCCGCAGAAAAAAA‑CCGAT‑AA‑ATCCCAACAG‑AAATGACCAACCCACACGCACCAGCAGAGGGGGGTCTTTAcc                                                        >  1:935040/1‑150 (MQ=255)

                                                                                                cACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCAcc                                                        <  2:230097/150‑1 (MQ=255)

                                                                                                    aTAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTTAATGGTTTTAGGCCGCCCC‑GCCGCAGCCGAACAAAA‑ACGAT‑AA‑AGCCAAACAA‑AAATGAACAACCCAAACGCCCCAGCCGAggggggtgtttcacctccc                                                   >  1:257478/1‑137 (MQ=255)

                                                                                                    aTAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGGTTTAGGCCCCCCC‑GCGCCCGCAGAACAAAA‑ACCAT‑AA‑TGCCCACCAG‑AATTTACCAACCCAAACGCACCCGCAGAGGGGGGTCTTTACCATccc                                                   >  2:851822/1‑149 (MQ=255)

                                                                                                         gTTGACGAAGAACCGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAAACGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATT‑CCGAT‑AA‑TGCCAACCAG‑AATTGGCCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTAc                                               <  1:38734/150‑1 (MQ=255)

                                                                                                            gACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTc                                            <  1:1183087/150‑1 (MQ=255)

                                                                                                              cGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTcgcg                                         <  1:708248/151‑1 (MQ=255)

                                                                                                               gaagaaCAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTTAATAGGGTTTAGGGCGCCGC‑GCGCCCGCAGAACAATA‑CCCAT‑AA‑TGCCAACCAG‑AAATGACCAACCCATACGCCCCAGCAGAGGGGGGTCTTCTCCATCCCGGACtttccgg                                        >  1:803238/1‑146 (MQ=255)

                                                                                                               gaagaaCAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCgg                                        <  2:380036/151‑1 (MQ=255)

                                                                                                                 agaaCAGCGGGCATCCTCCGGCGATAATGGCGGTTTTAAAATGGTTCGAGGCC‑CCGC‑GCGCCCGCAGAACAAAA‑GCCAT‑AA‑AGACAAACAG‑AATTTGACAACAGCTACGCACCACCGGAGGGGGGTGTTAACCACCCCGttgctccccg                                        >  1:1168001/1‑140 (MQ=255)

                                                                                                                  gaaCAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCaa                                     <  2:463560/151‑1 (MQ=255)

                                                                                                                     cAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTg                                  <  1:580489/151‑1 (MQ=255)

                                                                                                                      aGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCTCCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTgg                                 <  2:333129/151‑1 (MQ=255)

                                                                                                                       gCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGa                                <  2:275938/151‑1 (MQ=255)

                                                                                                                         gggCATCCTCCGGCGATAATGGCGG‑TTTTAATCGGGTTCAGGCCGCCCC‑GCGCCCGCCGAACAAAA‑ACCAT‑AA‑AGCCCACCAG‑AAATGACCCACCCATACGCACCCGCAGAGGGGGGTCTTTACCCACCCGTACCTCCCGGGAAgggg                                 >  1:361283/1‑148 (MQ=255)

                                                                                                                          ggCATCCTCCGGCGATAATGGCGGTTTTAAATGGATTTCAGG‑CTCCCA‑GCGCCACCCGAACAATACCCCAT‑AA‑T‑CGCACCAA‑AAATTACCAACCAAAACACCCCCCCACAGGGGGTTCTTCACCAACCCGTAATTCTCGGCCATAGGACa                              >  2:653236/1‑150 (MQ=255)

                                                                                                                           gCATCCTCCGGCGATAATGGCGG‑TTTTAATTGGTTTTAGGCCGCCCA‑GCGCCAGCAGAACAATA‑CCCATAAA‑TTCCAA‑CAG‑AATTGACCAAACCCAACGCCCCAGCAGAGGGGGGTTTTTACCACCCCGCACTTCCCGGCacatgggcaa                             >  2:785921/1‑141 (MQ=255)

                                                                                                                            cATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAA‑AATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCg                           <  2:1155602/151‑1 (MQ=255)

                                                                                                                             aTCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTTAGGCCGCCGC‑GCGCCCGCAGAACAAAA‑CCGGT‑AA‑TGCCAACCAG‑AATTGACCCACCGCTACGCACCAGCAGAGGGGGTTCTTTACCATCGCGTACTTCGCGGCCAGGGGGCATCGc                          >  1:817741/1‑151 (MQ=255)

                                                                                                                                ctccGGCGATAATGGCGG‑ATTGAAACGGTGTCAAGCCCCCCA‑GCGCCAGCCGAAAAAAA‑ACCAT‑AATTGCAAACCAA‑AATT‑ACCCACCGATACGCACCAGCAGAGGTGGGTTTTCCCCATCCCGGACTTTGCGGCCAAGGGAAATCGcc                         >  1:306547/1‑149 (MQ=255)

                                                                                                                                  ccGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAgg                      <  2:265950/150‑1 (MQ=255)

                                                                                                                                      cGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGtata                 <  2:558245/151‑1 (MQ=255)

                                                                                                                                       gATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATaa                <  2:745767/151‑1 (MQ=255)

                                                                                                                                       gATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATaa                <  2:716592/151‑1 (MQ=255)

                                                                                                                                                    ttGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAAGAGCAGGCGtt     >  2:734754/1‑150 (MQ=255)

                                                                                                                                                      gAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAAGAGCAGGCGTTaa   <  1:224990/150‑1 (MQ=255)

                                                                                                                                                      gAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAAGAGCAGGCGTTaa   <  1:203751/150‑1 (MQ=255)

                                                                                                                                                      gAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAAGAGCAGGCGTTAAc  <  2:811783/151‑1 (MQ=255)

ATCATTTAAATTAAAATCCATCTTTCAACCTCTTGATATTTTGGGGGTTAATTAATCTTTCCAGTTCTGTTTCGCGTCTTTAATAAAGGAGAGCGTCACCATAATGTTGACGAAGAACAGCGGGCATCCTCCGGCGATAATGGCGG‑TTTGAATCGGTTTCAGGCCGCCGA‑GCGCCAGCAGAACAATA‑CCGAT‑AA‑TGCCAACCAG‑AATTGACCAACCGATACGCACCAGCAGAGGTGGTTCTTCACCATCGCGTACTTCGCGGCAAGTGGACATCGCCAGGGTATAAGAGCAGGCGTTAAC  >  NC_000913/40366‑40665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`