Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	51.1%	51.8 / 142.4	88	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (6/37);  new base T (45/0);  total (51/37)
Fisher's exact test for biased strand distribution p-value = 7.02e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.64e-12

CAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT  >  NC_000913/3206285‑3206581                                                                                                                                                         |                                                                                                                                                    cAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTc                                                                                                                                                     <  1:1191098/151‑1 (MQ=255)       aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑a                                                                                                                                              <  2:223336/151‑1 (MQ=255)        tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGG‑AATTTTTcag                                                                                                                                                   >  1:93923/1‑145 (MQ=255)          tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑aaa                                                                                                                                            <  2:981981/150‑1 (MQ=255)            gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATAc                                                                                                                                         <  2:416902/151‑1 (MQ=255)             cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTCCTGAC‑AAATAc                                                                                                                                         >  1:891131/1‑150 (MQ=255)               aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑G‑AATTTTTTCAGAC‑aaaaaaaa                                                                                                                                       >  1:1030320/1‑145 (MQ=255)                   aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACt                                                                                                                                  <  2:304404/151‑1 (MQ=255)                       ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑G‑GATTTTTTCCGAC‑AAATAaaaaaaacgg                                                                                                                                >  1:124234/1‑145 (MQ=255)                         aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGtt                                                                                                                            <  1:835066/151‑1 (MQ=255)                         aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGtt                                                                                                                            <  2:1061550/151‑1 (MQ=255)                          ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGAC‑AAAAAAAAAAAATGGAggt                                                                                                                            >  1:999714/1‑148 (MQ=255)                          ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGtt                                                                                                                            <  2:157435/150‑1 (MQ=255)                          ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGtt                                                                                                                            <  2:352807/150‑1 (MQ=255)                              cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCCA‑AC‑AAAAAAAAAAAATGGAGTTggcct                                                                                                                       >  1:1119924/1‑147 (MQ=255)                              cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCCGAC‑CAATAAAAAAAATGGAGtttg                                                                                                                          >  2:952068/1‑146 (MQ=255)                                gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑GAATTTTTCAGAC‑AAAAACAAAAAATGGAGTTGGCat                                                                                                                       >  2:41643/1‑149 (MQ=255)                                gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGAC‑AAAAAAAAAAAAAGGAGGTGcc                                                                                                                         >  2:890041/1‑147 (MQ=255)                                gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCatga                                                                                                                     <  1:878838/151‑1 (MQ=255)                                gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCat                                                                                                                       <  2:349148/149‑1 (MQ=255)                                   ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCatgat                                                                                                                    <  2:163098/149‑1 (MQ=255)                                     ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                                 <  1:725091/150‑1 (MQ=255)                                      gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGAA‑AAAAAAAAAAAAAGGAGTTGGCATTATGAggg                                                                                                               >  2:355621/1‑149 (MQ=255)                                       cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGAC‑AAAAAAAAAAACTGGAGGTGCCATGATGAGCg                                                                                                               >  2:192636/1‑150 (MQ=255)                                       cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                              <  2:255561/151‑1 (MQ=255)                                         cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGaaa                                                                                                            <  2:479297/151‑1 (MQ=255)                                         cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTATTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGaaa                                                                                                            <  2:181486/151‑1 (MQ=255)                                         cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGC‑G‑AATTTTTTTAGAA‑AAAAAAAAAAAATTGAGGTggaatg                                                                                                                      >  1:650272/1‑136 (MQ=255)                                            tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCAGAC‑AAAAACAAAAAAAGGAGGTGTCATGATGAGCGgaa                                                                                                            >  1:921396/1‑148 (MQ=255)                                             attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaa                                                                                                        <  2:309661/151‑1 (MQ=255)                                                   tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑GATTTTTTTAAAC‑AAAAAAAAAAAATGGGGTTGTCATGGTGAGGGGAAGaaataa                                                                                                     >  1:614100/1‑148 (MQ=255)                                                    cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGGAATAAACa                                                                                                  >  2:317205/1‑150 (MQ=255)                                                      tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCaa                                                                                                 <  1:778357/149‑1 (MQ=255)                                                      tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc                                                                                                <  2:932636/150‑1 (MQ=255)                                                       aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGAC‑AAAAAAAAAAAATGGAGTTGCCATGATGAGCGGAAGTAATAAacaaca                                                                                               >  2:504128/1‑150 (MQ=255)                                                         tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑GATTTTTTCAGAA‑AAATACAAAAAATGGAGTTTGCATGGTGAGCGAAAGGAATAAACaaaa                                                                                               >  1:248082/1‑146 (MQ=255)                                                         tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                             <  2:601254/150‑1 (MQ=255)                                                            cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAg                                                                                          <  2:1088745/150‑1 (MQ=255)                                                             gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTg                                                                                        <  1:404181/151‑1 (MQ=255)                                                              cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGAC‑AAATACAAAAACAGGAGGTGCCATGATGAGCGGAAGTAATAAGCAAAAGGCAg                                                                                          >  1:921583/1‑148 (MQ=255)                                                                 tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑GATTTTTTCAGAC‑AAAAAAAAAAAAAGGGGTTGGCATTATGAGCGAAAGAAATAAACaa                                                                                                 >  1:826892/1‑138 (MQ=255)                                                                 tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt                                                                                     <  2:966831/150‑1 (MQ=255)                                                                 tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTTATAC‑AAAAACAAAAAATGGAGATGTGCTTATGTGTGCAAATAATAAAAAAAAGGCAgag                                                                                        >  1:120061/1‑145 (MQ=255)                                                                   aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCTGAC‑AAATACAAAAAATGGGGTTTGCATTATGAGGGGAAGTAATAAAAAACAAGCAGGGAaaaa                                                                                   >  1:159480/1‑150 (MQ=255)                                                                   aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAAACAAAAAAAAAAAATTGGGGTTG‑CATTATGAGCGGAAAGAATAAAAAAAAAGCAGGGAaaaa                                                                                   >  1:706333/1‑150 (MQ=255)                                                                   aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCAGAC‑AAAAAAAAAAAACGGAGGTGGCATGATGAGCGAAAATAAAAAACAAAAGGGAgag                                                                                        >  2:1144567/1‑143 (MQ=255)                                                                    ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAg                                                                                  <  2:891139/150‑1 (MQ=255)                                                                      cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCGGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGtt                                                                                <  1:577038/150‑1 (MQ=255)                                                                        cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑GAATTTTTCAGAC‑AAAAACAAAAAAATGGGTTGTCCTTATTAGGGAAAGGAATAAAAAACAAGCAGGGGATaa                                                                                   >  2:487731/1‑145 (MQ=255)                                                                        cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGAC‑AAAAAAAAAAACTGGAGGTGTCATGATGAGCGGAAGGAATAAACAACAGGCAGGGAaaaaaat                                                                                >  2:1194298/1‑145 (MQ=255)                                                                          aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑T‑AAGATTTTCACAC‑GAAAAAAAAAAAGGGAGGTGCCCTGAGGAGGCATAGAAATAAGGaaaaacgaatgaata                                                                                    >  1:1126234/1‑129 (MQ=255)                                                                            cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑CATTTTTTTTGAT‑AAAAAAAAAAACCTGGGGTGCCCAGACGGGGGAAAATAATAAGAAACACGccaggaaaaaggtta                                                                              >  2:1203088/1‑132 (MQ=255)                                                                             tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCAAAC‑AAAAAAAAAAAATGGAGTTTCCATGATGGGCGAAAGTAAAAAAAAACAAGCAGGGAATAAGTTGaaa                                                                            >  1:1025685/1‑145 (MQ=255)                                                                              gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACagag                                                                         <  1:1166180/149‑1 (MQ=255)                                                                                 aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGA‑G‑AATTTTTTCAAAC‑AAATACAAAAAACTGAGTTGACAAGATAAGCGGAAGTAAAAAGAAACAGGGAGGGAATaaaatgtgagggattat                                                                    >  2:363723/1‑136 (MQ=255)                                                                                   ttACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGAC‑AAAAAAAAAAACTGGGGTTGCCATGATGAGGGAAAGTAATAAACAAAAAGCAGGGAATAAAATGGAAGAGAATGTCg                                                                  >  2:150233/1‑151 (MQ=255)                                                                                    tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCCTAA‑AAAAAAAAAAAAAGGGGTTGGCATGGTGGGCGAAAGAAATAAAAAACAGGGAGGGAA‑AAGTTGACAGAGATTGTTGcc                                                                >  1:445094/1‑150 (MQ=255)                                                                                    tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCg                                                                  <  2:921404/150‑1 (MQ=255)                                                                                       atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTTAGAA‑AAAAAAAAAAAATTGAGGTGTCATGATGAGCGGAAGTAATAAAGAACAAGCAGTGAATAAAATGGCAGAGATTggtgct                                                                >  2:481810/1‑144 (MQ=255)                                                                                       atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑GAAATTTTTTCA‑AC‑AAAAACAAAAAATGGAGTTGGCATGGTGGGGGGAAGTAATAAACAACAGGGAGTGGATAAATTGTCAGAGATTGtt                                                                   >  1:174228/1‑145 (MQ=255)                                                                                         atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACt                                                            <  1:1102629/151‑1 (MQ=255)                                                                                          ttCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGAC‑AAATACAAAAAATGGAGTTGCCACGATGAGCGGAAGTAATAAACAACAAGCAAGGAATAAATTGACAGAGATTGTCGCTAACtt                                                           >  2:88018/1‑151 (MQ=255)                                                                                          ttCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGAC‑AAAAAAAAAAAATGGAGGTGCCATGATGGGGGAAAGTAAAAAACAACAAGCAGGGAAAAAATTGAAAGAGATTTTCGCTAAcat                                                           >  1:77802/1‑149 (MQ=255)                                                                                           tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACtt                                                           <  2:569144/150‑1 (MQ=255)                                                                                             aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAc                                                        <  2:481218/151‑1 (MQ=255)                                                                                              cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑G‑AATTTTTTCACAC‑AAATAAAAAAACTTGGGGTACCCTCATGAGGGGAAGGAAAAAAAAACAAGCAGGGGATAAGTTGACAGAGATTGTCGGTaaat                                                            >  1:177958/1‑144 (MQ=255)                                                                                               ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCg                                                      <  2:1077434/151‑1 (MQ=255)                                                                                                gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCAAAA‑AAAAAAAAAAAATGGGGTTGGCATGGTGGGGGGAAGTAATAAAAAAAAGGCAGAGAAAAAATTGACAGAGATTGTCGCTAAATTTAcccc                                                     >  1:696720/1‑149 (MQ=255)                                                                                                 tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCCGAC‑AAATAAAAAAAATGGAGGTGCCATGATGAGCGGAAGGAATAAACAAAAAGCAGGGAAAAAGTTGACAGAGATTTTTGCTAAATTTTCcccc                                                    >  2:641940/1‑151 (MQ=255)                                                                                                   gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑G‑AATTTTTTCAAAC‑AAAAAAAAAAAATGGAGTTGGCATGATGAGGGAAAGTAATAAACAACAAGCAGTGAATAAATTGACAGAGAGTTTCGCTAACTTTTCcccc                                                    >  1:670475/1‑149 (MQ=255)                                                                                                    cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCAGAC‑AAAAAAAAAAAATGGAGTTTGCATGATGAGGGAAAGTAAAAAAAAAAAAGCAGTGAAAAAAATGAAAGAGATTGTCGCTTAATTTACCCCCCTg                                                 >  1:471422/1‑151 (MQ=255)                                                                                                    cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑G‑AATTTTTTTAAAT‑AAATAAAAAAAAAGCTGGTGCCGTGATTGGGGAAAGGAATAAAAAACAAGCAGGGAATAAAGTGAAAAAGAATGTCTCTAACattttcc                                                      >  1:1078733/1‑142 (MQ=255)                                                                                                         aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCACAA‑AAAAACAAAAACTGGAGTTGTCCTGATGAGCGAAAATAAAAAACAAAAAGCAGTGAAAAAATTGACAAAGAATGTCGCGAACTTTTCCCCCAAGAttt                                             >  2:1185982/1‑150 (MQ=255)                                                                                                          atatAAAACCACATTTTTAGTGGTAGTTTGTGGGGGT‑G‑AATTTTTCCAGAA‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGAAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCt                                           <  2:601434/151‑1 (MQ=255)                                                                                                          atatAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACGGGAGTGGCAATGATGACCGAAAGAAATAAGCAACAGGCAGTAAAAAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCt                                           <  2:655785/151‑1 (MQ=255)                                                                                                            ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGAC‑AAAAACAAAAAATGGAGTTGCCATGATGAGGGAAAGGAATAAACAACAGGCAGTGAATAAGTTGACAGAGATTTTCGCTAAATTTATCGCCCTGAttttt                                           >  2:1100589/1‑147 (MQ=255)                                                                                                             taAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGAC‑AAAAACAAAAAATGGAGGTGGCATGATGAGCGAAAGGAATAAACAAAAGGCAGTGAATAAATTGACAGAGATTGTCGCTAAATTTAACCCCCTGAttt                                             >  1:764327/1‑146 (MQ=255)                                                                                                               aaaCCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGAC‑AAAAACAAAAACTGGAGTTGCCATGATGGGCGAAAGTAATAAACAACAGGCAGGGAATAAAATTACAGAGATTGTCGCTAACTTTACCGCCATGATTTTTAccc                                       >  1:239565/1‑150 (MQ=255)                                                                                                                   cacaTTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCCGAC‑AAATAAAAAAACTGGAGGTGCCCTGATGGGCGGAAGGAAAAAGAAAAAAGCAGGGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCCaa                                    >  2:203018/1‑149 (MQ=255)                                                                                                                      aTTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGAC‑AAAAAAAAAAAAAGGAGTTGCCATGGTGAGGGGAAATAATAAAAAAAAAGCAGGGAAAAAATTGACAGAGAATGTCGCTAACTTTAACCCCATGATTTTTACCCCAATAcc                                >  2:101846/1‑150 (MQ=255)                                                                                                                       tttttAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCt                               <  2:168321/150‑1 (MQ=255)                                                                                                                             gTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAA‑AAATACAAAAACGGGATTTGCCATGATGAGCAAAAGAAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACg                        <  2:839928/151‑1 (MQ=255)                                                                                                                               ggTAGTTTGTGGCGGT‑A‑AATTTTTCAAGAC‑AAATACAAAAACTGGAGTTCCCATGATGAGCGAAAGAAATAAGCAACAGGCAGTAAAAAAGTTGACAGAGTTTGTCGCTATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                      <  1:990583/151‑1 (MQ=255)                                                                                                                                 tAGTTTGTGGCGGT‑G‑AATTTTTTCCGAC‑AAAAACAAAAAATTGAGTTGCCATGATGAGCGGAAGTAAAAAACAACAAGCAGAGAAAAAATTGACAGAGATTGTCGCTAACTTTAACCCCCTGATTTTTTACCGAAAGCCCGATGACgggg                     >  1:139663/1‑150 (MQ=255)                                                                                                                                       gtgGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCACCAGGCAGTGAATAAGTTGACAGAGATTGTCGCTATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt                <  1:577664/149‑1 (MQ=255)                                                                                                                                            ggT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa          >  2:112409/1‑150 (MQ=255)                                                                                                                                                g‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc      >  2:1181233/1‑151 (MQ=255)                                                                                                                                                  aaTTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc      >  2:615934/1‑150 (MQ=255)                                                                                                                                                    tttttCCAGAA‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAg    <  2:1119934/150‑1 (MQ=255)                                                                                                                                                     ttttCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt  >  1:445964/1‑151 (MQ=255)                                                                                                                                                         |                                                                                                                                                    CAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAC‑AAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT  >  NC_000913/3206285‑3206581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑