Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,443,277	0	A	C	44.9%	106.9 / 25.4	69	V274G (GTA→GGA)	secY	preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (35/3);  new base C (0/31);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 1.50e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACT  >  NC_000913/3443127‑3443426                                                                                                                                                         |                                                                                                                                                      gCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTa                                                                                                                                                       <  2:75505/151‑1 (MQ=255)  cAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTAc                                                                                                                                                      <  2:399503/151‑1 (MQ=255)        cATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGc                                                                                                                                                >  2:118734/1‑151 (MQ=255)          tAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGcc                                                                                                                                               >  1:157007/1‑150 (MQ=255)           agagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCa                                                                                                                                              <  1:393461/150‑1 (MQ=255)               tAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTc                                                                                                                                         >  2:584153/1‑151 (MQ=255)                          ggggTTGCCCAGGCTGCAAATACAGCGAATTTGTTCCCACCCGGTCCCAA‑CCGGTACCGCCCCCGACCCATGACGGGAGGTTCGCCGGGAAAAAAATAATACGGG‑AAGCAAAGATTGCCGGAATCCCCCCCGCCATATTCACTTTCAGCgg                                                                                                                              <  2:387636/149‑1 (MQ=255)                            gtttccccAGGCTGAAAATACAGCAAATTTTTTGCCAGCCGTTCCCAC‑CCAGTCCCGCCCCCGAACCATGACGCGTTGGTCGCCGGAAAAAAAATAATACTGG‑AAGAGAAGTTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTa                                                                                                                            <  2:65457/146‑1 (MQ=255)                            ggTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCgg                                                                                                                              >  2:107430/1‑149 (MQ=255)                            ggTTCCCCGGGCTGCAAATACAGCAAAATTTTTGCCAGCCAGTTCCAA‑CCAGTCCCGCCCCCGACCCATGACGCGATGGTCGCGGGGAACAAAATAATACTGG‑AAGCGAAGATTGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTa                                                                                                                            <  2:459665/151‑1 (MQ=255)                             gTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTa                                                                                                                            >  1:400347/1‑150 (MQ=255)                                 cccAGGCGGAAAAAACAGGAAATTTGTTGTCGGCCATTCCCAC‑CCAGTCCCGCCCCCGAACCATGCCGCGATGGTCGCGGGGAAAAGAAAAATACGGG‑AGGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGt                                                                                                                             <  2:811496/145‑1 (MQ=255)                                    aGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtgtg                                                                                                                    >  1:570298/1‑151 (MQ=255)                                     ggCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGc                                                                                                                   >  1:1041959/1‑151 (MQ=255)                                      gCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGct                                                                                                                  >  1:869545/1‑151 (MQ=255)                                           aaaTACAGCAAATTTTTTGCCAGCCA‑TTCCAACCCAGTCCCCCCCCCGACCCAGGCCGCGATGGCCGCGGGAAACAAAATAATACTGG‑AAGCGAAGATGGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctct                                                                                                                <  2:736617/148‑1 (MQ=255)                                            aaaccagaaaagttgttgCCAGCCAGTTCCAC‑CCACCCCCCCCCCCAAACCATGCCGCGAGGGCCGCGGGAAAAAAAATAATACTGG‑AAGGGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                               <  1:781275/136‑1 (MQ=255)                                            aaaaCAGCAAATTTTTTGTCCCCCA‑TTCCAACCCAGTCCCCCCCCCAACCCATGACGCGAGGTTCGCGGGAAACAAAATAATACTGG‑AAGCAAAGTTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgc                                                                                                            <  2:797664/148‑1 (MQ=255)                                                    gAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                       >  2:68061/1‑148 (MQ=255)                                                      aaTTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                                   >  2:758769/1‑150 (MQ=255)                                                       aTTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGacac                                                                                                 >  1:367027/1‑151 (MQ=255)                                                        tttttgtCACCTATTCCCAC‑CAAGTGCCGCCCCCGACCCAGAACGGGATGGGCGCGGGAAAAAAAAAAATAGTGG‑AAGGGAAGTGGGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGacac                                                                                                 <  1:897439/147‑1 (MQ=255)                                                           ttgtCCGCCATTCCCAC‑CCAGTCCCCCCCCCGAACCATGCCGCGATGGTCGCCGGAAACAAAATAATACTGG‑AAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                              <  2:999163/150‑1 (MQ=255)                                                            tgccacccAGTCCAAC‑CCAGTCCCCCCCCCAACCCATGCCGCGTTGGTCGCCGGGAACAAAATAATCCTGA‑AAGCGAAGATGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                              <  1:464760/143‑1 (MQ=255)                                                              tCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGAcc                                                                                          >  2:117687/1‑151 (MQ=255)                                                               cAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCt                                                                                         >  2:400901/1‑151 (MQ=255)                                                                aGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                        >  1:702362/1‑151 (MQ=255)                                                                  ccattcccAC‑CCAGTCCCCCCCCCAAACCATGCCGCGAGGGTCGCGGGAAACAAAATAATACGGG‑AAGCGAAGTTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                        <  2:875512/143‑1 (MQ=255)                                                                   cAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCt                                                                                     >  1:384523/1‑151 (MQ=255)                                                                          aa‑CCATTCCCCCCCCCAACCAAGGCCGCGATGGTCGCGGGAAAAAAAATAATACTGG‑AAGCAAAGATTCCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                              <  2:213489/151‑1 (MQ=255)                                                                          aa‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                              >  2:690715/1‑151 (MQ=255)                                                                             ccAGTCCCCCCCCCGACCCTTCACGCGAGGCTCGCGGGGAAAAAAATAATCCTGG‑AAGAAAATATTGCGGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTc                                                                             <  2:482898/150‑1 (MQ=255)                                                                               aGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgcg                                                                          >  1:777069/1‑151 (MQ=255)                                                                                 tACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt                                                                         >  1:606508/1‑150 (MQ=255)                                                                                  ccccccccatacccATGAGGCGATGGTCGCGGGGAACAAAATAATACTGG‑AAGCAAAGATTGCCGGTTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAg                                                                       <  1:612150/139‑1 (MQ=255)                                                                                   ccccccccAAACCAGGCCGCGTTGGCCCCCGGAACAAAAATAAT‑CTGGAAAGCAAATTTTCCCGGATTTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTc                                                                             <  1:340207/141‑1 (MQ=255)                                                                                    cccccccAAACCAGGACGCGATGGTCGCCGGGAACAAAAAAATACTGG‑AAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGtt                                                                     <  2:58626/149‑1 (MQ=255)                                                                                       ccccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATTTTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAc                                                                  >  2:592861/1‑151 (MQ=255)                                                                                       ccccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAc                                                                  >  2:705420/1‑151 (MQ=255)                                                                                       ccccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAc                                                                  >  2:427484/1‑151 (MQ=255)                                                                                             aCCATGACGCGATGGGCGCCGGAAACAAAAAAATACGGG‑AAGCGAAGTTTGCCGGATTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcaca                                                              <  1:510898/149‑1 (MQ=255)                                                                                              ccatggcggggggggcgcgGGGAACAGAATAATAGTGG‑AGGCGAAGATTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATg                                                           <  1:493823/136‑1 (MQ=255)                                                                                                aTGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcg                                                         >  1:656761/1‑151 (MQ=255)                                                                                                  gACGGGATGGTCGCGGGAAAAAAAAAAATACGGG‑AAGGGAAGTTTGCCGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAAt                                                            <  2:808046/146‑1 (MQ=255)                                                                                                   aCGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggcg                                                      >  1:863100/1‑151 (MQ=255)                                                                                                    cgcgATGGTCGCCGGGAACAAAATAATACTGG‑AAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggcg                                                      <  2:672725/150‑1 (MQ=255)                                                                                                     gcgATGGCCGCGGGAAAAAAAATAATACGGG‑AAGAGAAGTTGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggcg                                                      <  1:778645/149‑1 (MQ=255)                                                                                                      cgATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTg                                                   >  1:122725/1‑151 (MQ=255)                                                                                                          ggTCGCGGGAAAAAGAAAAATACTGG‑AAGCGAAGTTTCCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTg                                                   <  1:721201/147‑1 (MQ=255)                                                                                                          ggTCGCGGGAAAAAAAATAATGCTGG‑AAGCGAAGATGGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTg                                                   <  1:124394/147‑1 (MQ=255)                                                                                                          ggTCGCCGGAAAAAAAATAATACTGG‑AGGCGATGATGGCCGGTTTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTg                                                   <  1:893590/147‑1 (MQ=255)                                                                                                           gTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCAc                                              >  1:120958/1‑151 (MQ=255)                                                                                                                 ggaaaaaaaataatGCTGA‑AAGCGAGGATTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTca                                         <  1:962913/142‑1 (MQ=255)                                                                                                                   gAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaac                                       >  1:516854/1‑150 (MQ=255)                                                                                                                      aaaaataatACCGA‑AAGCAAATATTGCCGGCCTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaacaa                                     <  1:781860/146‑1 (MQ=255)                                                                                                                       aaaataatACTGG‑AGGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACaaa                                    <  1:108334/147‑1 (MQ=255)                                                                                                                       aGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                   >  2:828486/1‑150 (MQ=255)                                                                                                                        aaaaaaggattgaaaGCGAAGTTTGCGGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                  <  2:684190/138‑1 (MQ=255)                                                                                                                         aataatACGGG‑AGGCAAAGTTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                   <  2:301204/148‑1 (MQ=255)                                                                                                                            aataCTGA‑AAGCAAAGTTTGCGTGTATACCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                              <  1:946674/150‑1 (MQ=255)                                                                                                                             ataCTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaaca                             >  1:536228/1‑150 (MQ=255)                                                                                                                                          aaGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTg                >  2:556779/1‑151 (MQ=255)                                                                                                                                          aaGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTg                >  2:808434/1‑151 (MQ=255)                                                                                                                                              ttGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaa             >  1:776139/1‑150 (MQ=255)                                                                                                                                                 ccGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac         >  1:278807/1‑151 (MQ=255)                                                                                                                                                  ggttaTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac         <  2:985198/146‑1 (MQ=255)                                                                                                                                                  cGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact        >  2:606601/1‑151 (MQ=255)                                                                                                                                                   ggtataaCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaaa            <  2:157028/140‑1 (MQ=255)                                                                                                                                                   gggATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaata          <  2:639160/148‑1 (MQ=255)                                                                                                                                                        tACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaatact  >  1:988236/1‑151 (MQ=255)                                                                                                                                                        tACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaatact  >  1:662931/1‑151 (MQ=255)                                                                                                                                                         |                                                                                                                                                      GCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGG‑AAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACT  >  NC_000913/3443127‑3443426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑