Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	659,940	0	T	A	45.9%	70.0 / 44.0	61	I93F (ATT→TTT)	lipA	lipoate synthase
Reads supporting (aligned to +/- strand):  ref base T (4/29);  new base A (28/0);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 2.55e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTCATCACGGTCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAAT‑G‑CA‑GGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTG  >  NC_000913/659796‑660088                                                                                                                                                 |                                                                                                                                                         ggTCATCACGGTCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAgcgc                                                                                                                                                     <  1:1157811/150‑1 (MQ=255) ggTCATCACGGTCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGcc                                                                                                                                                    <  2:446221/151‑1 (MQ=255)     atcaCGGTCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAAAGCCGAgg                                                                                                                                                >  2:205274/1‑151 (MQ=255)       caCGGTCAACGGAGGTGATAACCATATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGa                                                                                                                                               <  2:960966/150‑1 (MQ=255)               aCGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACg                                                                                                                                      <  1:823494/151‑1 (MQ=255)                  gAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAACGCCCAGGGACATaaaaatt                                                                                                                                    >  2:963829/1‑146 (MQ=255)                        aTAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAAAGCCGacgggcattaacag                                                                                                                                     >  1:841396/1‑130 (MQ=255)                          aaCCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑g                                                                                                                          <  2:1215864/151‑1 (MQ=255)                           aCCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑Gt                                                                                                                         <  2:522685/151‑1 (MQ=255)                                 tAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTg                                                                                                                    <  1:55359/150‑1 (MQ=255)                                 tAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAAAAACGCCCAGGGACATAAACAGTGCTTTGCC‑GGGGGTgg                                                                                                                   >  1:578589/1‑150 (MQ=255)                                   aCGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAg                                                                                                                 <  2:609825/151‑1 (MQ=255)                                     gCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAGAGCCCAGGAGCATAAAAGATGCTGTGTC‑GGGGGTg                                                                                                                    >  1:800269/1‑146 (MQ=255)                                     gCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAACGGCGGGGGACCTAAAAGATGGTGTGCC‑gggggt                                                                                                                     >  2:16638/1‑143 (MQ=255)                                     gCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAAAGCCGAGGGACATaaaaacttct                                                                                                                                 >  2:235287/1‑127 (MQ=255)                                          gcCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAAAGCCCCGGAGCATAAAAGCTGCTGGGCC‑GTGGGTGTAGCAATCCg                                                                                                          >  1:376930/1‑151 (MQ=255)                                            cATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGcc                                                                                                        <  2:1102194/151‑1 (MQ=255)                                            cATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAACGCCCCGGGTCATAAAAGTTGCTGTGCC‑GGGGGTGGAGCATTCCGcc                                                                                                        >  1:484758/1‑151 (MQ=255)                                             atatCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAAAAAAGCCCAGGATCATAAAAATTGGTGTGCC‑GTGGTTGGAGCATTTCGcc                                                                                                        >  2:326667/1‑150 (MQ=255)                                               atCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAg                                                                                                      <  2:177572/150‑1 (MQ=255)                                               atCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAAGGGCGCGGGGCATAAAAGTTGCTTTGGC‑GGGGGTGAAGGAtttcccccgg                                                                                                     >  2:295033/1‑143 (MQ=255)                                                 cGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGGAAAAAAACGCCGAGGATCATAAACACTTCTTTGGC‑GGGGGTGGAGCATTCCCCCCgg                                                                                                     >  1:450680/1‑149 (MQ=255)                                                  ggCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTa                                                                                                  <  2:1145855/151‑1 (MQ=255)                                                   gCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAg                                                                                                 <  2:519612/151‑1 (MQ=255)                                                   gCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAACGCCGCGGGTGCTAAAACTTGCTGTGGC‑GCGGGTGGAGCAATCCGCCCggg                                                                                                    >  2:171054/1‑147 (MQ=255)                                                     aaTGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAggg                                                                                               <  2:1241283/151‑1 (MQ=255)                                                     aaTGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAACGCCGAGGGGCATAAAAGGTGCTGTGGC‑GGGGGTGGAGCATTCCCCCCCGGTAggg                                                                                               >  2:815925/1‑151 (MQ=255)                                                             gcgcCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCagga                                                                                          <  1:1110699/148‑1 (MQ=255)                                                             gcgcCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGc                                                                                       <  2:1110369/151‑1 (MQ=255)                                                                    tttCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAAAAAAGCCGCGGGGGAAAAAAGTTGCTGTGCC‑GTGGGTGGAGCATTTCGCCCGGGTAggggggggggggtcctc                                                                                 >  2:37965/1‑136 (MQ=255)                                                                       cACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGca                                                                              <  2:1071758/150‑1 (MQ=255)                                                                       cACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGca                                                                              <  1:142511/150‑1 (MQ=255)                                                                           ggTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGcagaca                                                                          <  2:690963/150‑1 (MQ=255)                                                                           ggTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAAAGCCGAGGGTCATAAAAGATGCTGTGCC‑GTGGGTGGAACATTCCGCCCGGGTTGGGGAGGAGGGCTTCTCGcagac                                                                           >  2:245648/1‑149 (MQ=255)                                                                                aTTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAAT‑G‑c                                                                  <  1:557915/151‑1 (MQ=255)                                                                                  tGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAGCGCCGAGGGTCATAAAAAATGCTGTGCC‑GGGGTTGGAGCAATTCGCCCGGGTAGGGGAGGGGGGGTCCTCGCCGACaaaa                                                                       >  1:36199/1‑146 (MQ=255)                                                                                   ggCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAACGCCGAGGATCATAAAAGGTGCTGTGCC‑GTGGGTGAAGCATTTCGCCCGGTTAGGGCAGGGGGGTTCCTCGcccgcaga                                                                        >  2:154741/1‑137 (MQ=255)                                                                                    gCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAAAGCCGAGGGACCTAAACAATGCTGTGCC‑CGGGGTGGAGCATTCCCCCCGGGTTGGGGAGGGGGGTTCCTCGCCGACAGaaaaca                                                                   >  1:716647/1‑144 (MQ=255)                                                                                            aGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAAT‑G‑CA‑GGCCATTTTTg                                                     <  2:865377/151‑1 (MQ=255)                                                                                              cTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAAAAACGCCCAGGGGCAAAAACATTGCTGTGGC‑CGGGGTGAAGGAATCCCCCCCGGTGGGGGAGGGGGGGTCCTCCCCGGCCAAAA‑G‑CAGGGCCAttttt                                                      >  1:30980/1‑149 (MQ=255)                                                                                                aCCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAAAAAAGCCGCGGGGCATAAAAGTTGCTGTGCC‑GTGGGTGGAGCATTTCCCCCGGGTAGGGGAGGAGGGCTTCTCCCCGGCAGAAA‑G‑GA‑GGCCCttttt                                                      >  2:80357/1‑146 (MQ=255)                                                                                                  cGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGGCAAAAAACGGCCGGGGGCAAAAAAGTTGTCTTGGC‑GGGGGTGAAGGATTTCGCCCGGGTAGGGGGGGGGGGTTTCTCCCCGACAGAATGG‑CG‑GGCCtttttttgccct                                                >  2:403219/1‑145 (MQ=255)                                                                                                  cGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGAGAAAAAGCGCCCGGGGACATAAAAGTTTGTGTGTC‑GGGGGTGGAGGATTTCGCCCGGGTTGGGGAGGGGGGGTCCTCGCCGACAGAAT‑G‑CA‑GGGCAtttttttggcat                                               >  2:519373/1‑144 (MQ=255)                                                                                                     gCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAAT‑G‑CA‑GGCCATTTTTGCGCATTGCg                                            <  1:722216/151‑1 (MQ=255)                                                                                                       gACCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAGCGCCGCGGAACATAAAAGTTGCTGTGCC‑GGGGGTGTAGCAATTCGCCCGGGTAGGGGAGGGGGCGTCCTCGCCGACAGAAA‑G‑CA‑AGGCACTTTTtcgcccttcgg                                           >  1:830797/1‑143 (MQ=255)                                                                                                        aCCGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAAAGCCCAGGATCATAAAAGGTGCTGTGCC‑GCGGGTGAAGCATTCCCCCCGGGTAGGGGAGGGGGGTTCCTCCCAGACAGAAA‑G‑CA‑GGGCCTTTTTTTGCATTTCg                                            >  2:526849/1‑148 (MQ=255)                                                                                                         ccGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCACCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAAT‑G‑CA‑GGCCATTTTTGCGCATTGCGGCtt                                        <  2:894197/151‑1 (MQ=255)                                                                                                         ccGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAAGGCCCCCGAAAATAAAAGATACTCTGCCGGGGGTGGAGGCATTCCGCCCGG‑TAGGGGGGGAGGACTCCCCCCCGGCAGAAA‑G‑TA‑AGCCCGTTTTTCGCCTTGCgg                                           >  1:176957/1‑148 (MQ=255)                                                                                                          cGTGGGCAACATCACAGAACGGACAACGGCGGGGACAAAAAACGCCGCGGGTCATAAAAAATGCTGTGGC‑GGGGGTGGAGGATTCCGCCCGGGTAGGGGGGGGGGGGTCCTCCCAGACAGAAA‑GAAA‑GGCCtttttttgccttgggggttt                                        >  1:706239/1‑137 (MQ=255)                                                                                                           gTGGGCAACATCACAGAACGGACAACGGCGGGTGCAAAAAACGCCCAGGGGCGTAAACCCAGACGTGTC‑CTGGGTGCAACATTCCGCCCAGGTAGGGGGCGAGGCTTTCCCGCACAGACAAA‑G‑CA‑CGCCACTTTTgttcctt                                               >  1:755578/1‑136 (MQ=255)                                                                                                              ggCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAAT‑G‑CA‑GGCCATTTTTGCGCATTGCGGCTTTGATg                                   <  1:898837/151‑1 (MQ=255)                                                                                                                   cATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAAT‑G‑CA‑GGCCATTTTTGCGCATTGCGGCTTTGATGCCCTg                              <  2:426359/151‑1 (MQ=255)                                                                                                                   cATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAAT‑G‑CA‑GGCCATTTTTGCGCATTGCGGCTTTGATGCCCTg                              <  1:22983/151‑1 (MQ=255)                                                                                                                        caGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAAT‑G‑CA‑GGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATAc                         <  1:948696/151‑1 (MQ=255)                                                                                                                                   aCGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAAT‑G‑CA‑GGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTcc              <  2:1253949/151‑1 (MQ=255)                                                                                                                                     ggcggGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAAT‑G‑CA‑GGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCg             >  2:400733/1‑150 (MQ=255)                                                                                                                                      gcggGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAAT‑G‑CA‑GGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCt           >  2:766351/1‑151 (MQ=255)                                                                                                                                          gTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAAT‑G‑CA‑GGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGaa       <  2:692119/151‑1 (MQ=255)                                                                                                                                           tACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAAT‑G‑CA‑GGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGa        >  2:968055/1‑149 (MQ=255)                                                                                                                                              aaaTAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAAT‑G‑CA‑GGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCtt   <  1:455139/151‑1 (MQ=255)                                                                                                                                               aaTAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAAT‑G‑CA‑GGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTg  >  2:467999/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                         GGTCATCACGGTCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGTACAAATAGCGCCGAGGATCATAAACGTTGCTGTGCC‑GTGGTTGAAGCATTCCGCCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAAT‑G‑CA‑GGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTG  >  NC_000913/659796‑660088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑