Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	357,890	0	C	A	45.0%	57.8 / 33.3	60	L267F (TTG→TTT)	cynR	transcriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (22/11);  new base A (0/27);  total (22/38)
Fisher's exact test for biased strand distribution p-value = 1.44e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGA  >  NC_000913/357745‑358038                                                                                                                                                    |                                                                                                                                                         aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAAc                                                                                                                                                    <  1:192832/151‑1 (MQ=255) aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAAc                                                                                                                                                    >  2:803513/1‑151 (MQ=255)  aGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAAcc                                                                                                                                                   >  2:1002955/1‑151 (MQ=255)    cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAAccg                                                                                                                                                  <  2:1088880/150‑1 (MQ=255)     gTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAAccgcc                                                                                                                                                >  1:907089/1‑151 (MQ=255)     gTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAAccgcc                                                                                                                                                <  2:905316/151‑1 (MQ=255)     gTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAAccgc                                                                                                                                                 <  1:517911/150‑1 (MQ=255)            aTCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTtctct                                                                                                                                         >  1:1042438/1‑151 (MQ=255)               aGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTcc                                                                                                                                       >  2:1115605/1‑150 (MQ=255)                 gCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCagt                                                                                                                                    >  1:492135/1‑151 (MQ=255)                           ccGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCgggg                                                                                                                          >  2:1120553/1‑151 (MQ=255)                           ccGAAGCCCTGTGTCAGGGCCTCCCGTGATCCTTTCCCGCCACCACCCGCGCTTTTACCCAACGCC‑TTGTGCAAAAATGCT‑TTCGCGGCGCCTGTCTGCCAGTTTTTTTCCCGCCCCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCg                                                                                                                             <  1:346115/148‑1 (MQ=255)                              cagccttgtgTCGGCGGTTACCGTGATTCATTCCCCCCAACCCCCGCCCTTTTACCCACCGCG‑TTTTAAAAAATTCCC‑TGCGCGGGGGCTGTCTGCCAGCTTTTTTCCCCCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCa                                                                                                                        <  1:780243/144‑1 (MQ=255)                                gccttttgtCAGGGGCTCCCGTTTTCCTTTCCCCCCAACCCCCGCGCTTTTACCCACCGCA‑TTGTGAAAAATTCCC‑TCCGGGGGGGCTGTCTCCCAGTTTTTTTCCCGCCGAAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCaa                                                                                                                       <  2:189059/143‑1 (MQ=255)                                 cccTGTGTCGGCGCCTCCCGTGATTCATTCCCCCCAACACCCGCCAATTTACCAACCCCC‑ATGTGAAAAAATGCT‑TTGGGGGCGGCTTTCTGCCGGCTTTTTTCCCGCCGAAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAaga‑g                                                                                                                   <  1:970074/150‑1 (MQ=255)                                       gtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGGGA‑G‑aaa                                                                                                               >  1:1163795/1‑148 (MQ=255)                                        tCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCgggggcaaaggaaaa                                                                                                               >  2:70114/1‑138 (MQ=255)                                         cagcgccccccGTGTTTCTTCCCCCCCAACACCCGCGCTTTTACCCACCGCC‑ATGTGAAAAAAGCCC‑TTCGGGGCGGCTGTCGGCCAGTTTTTTTCCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCaa                                                                                                                       <  1:860479/131‑1 (MQ=255)                                             gcctcccGTGATTCATTTCCCCCAACAACCGCGCTTTTACCCACCGCC‑ATGTGAAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGTTATTTTCCCGCCGAAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTaa                                                                                                      <  2:343693/146‑1 (MQ=255)                                                tACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑CTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCCAGA‑G‑aaaaaaattttaaac                                                                                                   >  2:756624/1‑139 (MQ=25)                                                 cccgtttttCATTCCCCCCAACACCCGCGCATTTACCCACCCCC‑TTGTGAAAAAATGCC‑TCCGCGGCGGCTTTCTGCCAGTTTTTTTCCCGCCGAAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGc                                                                                                           <  1:634222/135‑1 (MQ=255)                                                  ccGTGTTTCATTCCCCCAAACAACCGCGCATTTACCCAACGCC‑TTGT‑AAAAAACGCCGTTCGCGGGGGGTCTCTGCCGCCTATTTTCCCGCCACAAA‑AAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGcc                                                                                                   <  2:486866/149‑1 (MQ=255)                                                  ccGTGATTCTTTCCCCCCAACACCCCCGCTTTTACCCACCCCC‑ATGTGAAAAAATCCC‑TTCGGGGGGGCTGTCTGCCAGTTTTTTTCCCCCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGcc                                                                                                   <  2:1147132/149‑1 (MQ=255)                                                   cGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑G‑AAAAAACTTTTAAccc                                                                                                  >  2:1011073/1‑149 (MQ=25)                                                     taaTTCTTTCCCCCCAACACCCGCGCTTTTATCCAACGCC‑ATGTAAAAAAATGCC‑TCCGGGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGAAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGt                                                                                                <  1:66639/147‑1 (MQ=255)                                                        ttCATTCCCGCCAACACCCGCGTTTTTA‑CCAACCCCAATGTGAAAAAAGCCT‑TTCGCGGCGTCTTTGTGCAAGCTTTTTTCCCCCCGAAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAtg                                                                                            <  2:1145357/150‑1 (MQ=255)                                                          cATTTCCGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGG‑G‑AAAAAGAATTAAGacccccagttcg                                                                                         >  1:155178/1‑142 (MQ=25)                                                          cATTCCCCCCAACACCCGCGCATTTACCAACCGCC‑ATGTGCAAAAATCCT‑TTCGCGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAtgt                                                                                           <  2:895164/149‑1 (MQ=255)                                                            ttcccccaaacaccCGCGCTTTTACCCAACGCC‑ATGTGCAAAAATCCC‑TCCGCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAtgttgt                                                                                        <  2:283485/142‑1 (MQ=255)                                                            ttcccGCCAACACCCGCGCTTTTACCCACCGCC‑ATGTGAAAAAATGCC‑TTCGGGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGAAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAtgttg                                                                                         <  2:1194130/146‑1 (MQ=255)                                                              cccGCCAACACCCGCGTATTTACCCACCGCC‑TTGTAAAAAATTGCT‑TCCGGGGGGGTGGTCTGCCAGTTTTTTTCCCGCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAtgttg                                                                                         <  1:1120003/146‑1 (MQ=255)                                                               ccccaaacaccCGCGCTTTTACCCAACGCC‑ATGTAAAAAAATGCT‑TGCGCGGCGGCTGTCTGCAAGTTTTTTTCCCGCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTtgt‑gtg                                                                                    <  1:931570/145‑1 (MQ=255)                                                                cGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAAA‑G‑AAAAAAATTTTAACCCCCCCTGTTTT‑GTggg                                                                                  >  2:1127865/1‑150 (MQ=25)                                                                cGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAAAAGCTTTAAACCCGCCCTGTTTT‑GTgg                                                                                   >  1:200887/1‑150 (MQ=255)                                                                  ccAACACCCGCGCATTTCCCCCACGCC‑ATTGGAAAAAATGCC‑TTCGCGGCGGCTTTTTGCCCGTTTTTTTCCCGCCCCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCa                                                                                 <  2:1009480/150‑1 (MQ=255)                                                                       aaccgcaattttACCAAACCCC‑ATGTCAAAAAATCCT‑TGCGGGGCGGTTTTCTCCCAGTTTTTTTCCCGCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCgc                                                                            <  1:836903/141‑1 (MQ=255)                                                                          cgcttttttACCCAACCCC‑ATGTACAAAAATCCC‑TTCGCGGCGCCTTTCTGCCAGCTTTTTTCCCGCCGAAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATc                                                                              <  1:879879/139‑1 (MQ=255)                                                                             gtaTTTACCCAACGCC‑ATGTGAAAAAGTGCC‑TGCGCGGCGGCTGTCTGCAAATTATTTTCCCCCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCGCTGCTGGt                                                                     <  2:907730/149‑1 (MQ=255)                                                                                       aaCGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGAGG‑AAAAAATTTTAACCCCG‑CATGTTGT‑TTGGGGATCGCTGCgggggaaaaagtgg                                                           >  2:349295/1‑140 (MQ=25)                                                                                        aCGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGG‑A‑AAAAAAATTTATACCCCTCCTTTTTT‑GGGGGGATCGCTGCTGGGAaaaaaatg                                                            >  1:379811/1‑145 (MQ=19)                                                                                         cGCC‑ATTTCAAAAATTGCT‑TCCGCGGGGGCTTCCTGCCAGTTTTTTTCCCGCCGAAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCGCTGCTGGTAACAATGTGGaa                                                         <  2:1168304/151‑1 (MQ=255)                                                                                              aTGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑G‑GAAAAAAATTAAACCCCTCCTGTTTT‑GTGGGAATCGCCGCTGGtgaaaaaatgg                                                           >  2:1095333/1‑135 (MQ=25)                                                                                               tgtaCAAAAATGCT‑TTGGCGCCGGTTGTCTCCCTTCTATTTTCCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCGCTGCTGGTAACAATGTGGAAAgg                                                      <  2:183160/149‑1 (MQ=255)                                                                                                gtgAAAAAATGCT‑TGCGGGGCGGCTGTCTCCCAGTTTTTTTCCCGCCACAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCGCTGCTGGTAACAATGTGGAAAggg                                                     <  2:719930/149‑1 (MQ=255)                                                                                                    aaaatTGCT‑TGCGCGCCGGTTTTCTGCCATTTTTTTTCCCGCCGAAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgc                                               <  2:699656/151‑1 (MQ=255)                                                                                                    aaaaaTGCC‑TTCGCGGCGGCTGTCTGCCAGTTATTTTCCCGCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGt                                                 <  2:445070/149‑1 (MQ=255)                                                                                                      aaaTGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAAAAAGTTTTAATCCCG‑CCTTTTGTGGGGGCAAT‑GCCGCTGGTGACAAAGTGGGAAGGGGAgggggg                                             >  2:245170/1‑146 (MQ=255)                                                                                                       aaTGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑A‑AAAAAAATTTTAACCCCCCCTGTTGT‑GTGGGGAACGCTGCTGGGGACAAAGTGGGAAGGGGAgggggg                                             >  2:289343/1‑145 (MQ=255)                                                                                                       aaTCCC‑TCCGGGGGGGTTGTCGCCCAGTTTTTTTCCCGCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcggc                                            <  1:246686/151‑1 (MQ=255)                                                                                                            ctttCGGGGCGGTTGTCTGCCAGTTTTTTTCCCGCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcggc                                            <  2:1066737/145‑1 (MQ=255)                                                                                                                   ggcggcTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGCGCGAGA‑GAAAAAAGTTTTAAACCCGCCATTTGTT‑TTGGCAACTCCGCCTGGTAAAAAATGGGAAGGGGAAGGGGGGGGAAtaactcc                                  >  2:421487/1‑145 (MQ=255)                                                                                                                      gccTGTCCCCCAGTTTTTTTCCCGCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGa                                          <  1:596731/137‑1 (MQ=255)                                                                                                                                ccattATTTTCCCCCCCCAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCgcgc                       <  1:585288/145‑1 (MQ=255)                                                                                                                                    ttttttcCCCCCGCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAAtt                  <  2:609120/147‑1 (MQ=255)                                                                                                                                    tATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGTGCGAGG‑G‑AAAAAAATTTTAAACCCTCCTTTTTT‑TTGGGGATCGCTGCTGGGGACAATATGGGAAGGGGAGGGGGGGGGATCAACCCCCCAACCGCGCCAAtt                  >  1:429274/1‑149 (MQ=255)                                                                                                                                      ttttcCCCCCGAAAC‑AAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAg               <  1:1267810/150‑1 (MQ=255)                                                                                                                                        tttCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGCGA‑G‑AAAAAAATTTAATCCCCCCATGTTGT‑GTGGGGAACGCTGCTGGGGAAAATGTGGGGAAGGGAGGGGGGGGGAGCAGCTCCACAAcccccccca                    >  2:994692/1‑136 (MQ=255)                                                                                                                                         tcccgccgCAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTc            <  1:548377/148‑1 (MQ=255)                                                                                                                                          cccgccgAAAA‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTc            <  2:733242/148‑1 (MQ=255)                                                                                                                                           ccgccgCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGcc         <  1:1196774/151‑1 (MQ=255)                                                                                                                                                  aac‑aaAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAAt    <  2:203455/149‑1 (MQ=255)                                                                                                                                                  aac‑aaAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGa  <  2:182838/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                         AAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCC‑ATGTGCAAAAATGCC‑TTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAAC‑AAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCATGTTGT‑GTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGA  >  NC_000913/357745‑358038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑