| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,678,039 | 0 | T | G | 45.6% | 82.9 / 19.8 | 57 | N108T (AAC→ACC) | yphE | putative sugar ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base T (5/26); new base G (26/0); total (31/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.63e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGC > NC_000913/2677899‑2678186 | tttGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCAGAGGTTTTCCGcc < 1:733423/149‑1 (MQ=255) gcCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACCGACCGA‑GGCCGAGGGTTTTCGCCCCTGTc > 1:15718/1‑150 (MQ=255) ccGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAg < 2:547852/151‑1 (MQ=255) cGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑GCACCGGCCGG‑GGGAGAGGGGTTTCGCCACTCTCAGc > 2:325417/1‑151 (MQ=255) cGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑GCACCGACCCA‑GGCAGGGGGTTTTCGcccc > 2:267182/1‑142 (MQ=255) gggCTTAGCGTTGAAACAAGTTGTTCAGGACTAGCGTCACCGCCCAGCGCCTGTAAGCAAGGTGGGGCATCCTGCGCCTTTTGCAGGTAATCAATCAGGCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCATAGGTTTTCCGCCACTGTCAg < 2:858648/149‑1 (MQ=255) ggCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACCGACCGA‑GGCAGAGGGTTTTCGCCCCCGTCAGccc > 2:61469/1‑151 (MQ=255) ttAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑GCCCTGACCGC‑GGCCGAGGGTTTTCGCCCCTGTCAGCCCtt > 2:868529/1‑150 (MQ=255) cGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTccacc < 1:471925/151‑1 (MQ=255) gTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACTCACCCC‑GGCAGAGGGTTTCCCCCACTGTCAGCCCTcccccc > 1:368473/1‑150 (MQ=255) tGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAg < 1:447288/150‑1 (MQ=255) gAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACTGACCGC‑GGCAGAGGGTTTTCGCCCCTGTCAGCCCTTCCCCCAGAc > 1:455909/1‑151 (MQ=255) aaGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAAc < 2:304405/151‑1 (MQ=255) aGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCCCCGACCCA‑GGGAGAGGGTTTCCGCCCCCGTCCGCCCCTCCCCCCGACtt > 1:676441/1‑147 (MQ=255) gttgttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCAACGACCGA‑GGCAGGGGGTTTTCGCCACCGTCAGCCCTTTCACCAGACttta > 1:781140/1‑146 (MQ=255) gttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACTGACCGA‑GGGAGAGGGTTTTCCCCACTGTCCGCCCCTCCCCCCGACTTAACTcc > 1:221936/1‑149 (MQ=255) gttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACCGACCGC‑GGGAAAGGGTTTTCGCCCCTGTCAGCCCCTCCCCCCGACtttaaccc > 2:539919/1‑143 (MQ=255) aGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCCCACCG‑‑GGCGGGGGGTTTTTGCCCCTGTCAGCCCCTCCCCCCGACTTCACTCCTGAt > 2:191234/1‑149 (MQ=255) gTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCCCTGGCCGG‑GGGGGAGGGTTTCCGCCACTGTCAGCCCCTTCACCCGACTTAAATCCCGATaaaaccc > 2:171352/1‑143 (MQ=255) cAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCAc < 1:299190/151‑1 (MQ=255) cGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCAcccc < 2:392284/151‑1 (MQ=255) cccAGCGCCTGTAAGCAACGTTGGTCATCCTGCCCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCACCCCCa < 2:205846/151‑1 (MQ=255) cccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCAccccc < 1:841659/150‑1 (MQ=255) gcgcCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCACCCCCAGtt < 1:212645/150‑1 (MQ=255) gcgcCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCACCCCCAGTTc < 2:249984/151‑1 (MQ=255) gcCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCCCTGACCCA‑GGGAGAGGGTTTTCGCCACCGTCAGCCCCTCCACCCGACTTAACTCCTGATAAAACGCC‑CGCCCCCCCCGtt > 1:123243/1‑148 (MQ=255) gcCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACCGAACGA‑GGGAGAGGGTTTCCTCCACCGTCATCCCCTCCCCCAGACTTTACTCCCGCTAAACCCCG‑CGCGccccccct > 2:83970/1‑144 (MQ=255) ccTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCCCTGACCGA‑GGCAGAGGGTTTTCCCCCCTGTCAGCCCCTCCACCAAACTTTACTCCTGATAAACCGCC‑CGCccccccccct > 1:769418/1‑143 (MQ=255) aaGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑GCCCCGACCGG‑GGGAGAGGGTTTCCGCCCCCGTCCGCCCCTCCCCCACACTTAAAACCTCATAAAACCCG‑CGCCCCCCCCGCTCAGCgg > 2:265413/1‑148 (MQ=255) aGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCCCCGACCGG‑GGGAGAGGGTTTTCGCCCCTGTCAGCCCCTCCCCCACACTTTACTCCCGATAAACCGCG‑CGCCacccc > 2:715282/1‑137 (MQ=255) cAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACCGACCGA‑GGCAGAGGGTTTTCGCCACCGTCAGCCCTTCCCCCCGACTTAAATCCTGATAAACCCCG‑CGCACCCCCCGTTCCGCGGCAc > 1:65570/1‑148 (MQ=255) aCGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACTGGCCGG‑GGGAGAGGTTTTTCGCCCCCGTCAGGCCTTCCACCCGGCTTTATACCTTATTAACCCCG‑CGCACCCCCAGCTCAGCGGccccg > 2:440430/1‑144 (MQ=255) gTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑GCACCGACCGAGGGCGCAGGTTTT‑CGCCCCCGTTCACCCTTCCACCCCACCTAACACCTTAAAAAACACGCCCCACCCCCACTTctccgga > 2:586042/1‑137 (MQ=255) tGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCCCTGACCGA‑GGCAGAGGGTTTTCCCCCCTTTCAGCCCCTCCCCCAGACTTAACTCCTGATAAACCCCG‑CGCACCCCCCCTTCAGCGGCCCGGCg > 2:371111/1‑146 (MQ=255) tGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACCGACCCA‑GGCAGAGGGTTTTCCCCCCCGTCAGCCCTTCCACCAGACCTAACTCCTGATAAACCCCG‑CGCACCCCCCGTTCAGCGGCACGGCGAGTc > 1:45667/1‑150 (MQ=255) gcgcCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTc < 2:578783/151‑1 (MQ=255) cATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑GCACCGACCGA‑GGCAGAGGGTTTCCGCCACTGTCAGCCCTTCCACCAGACCTAACTCCTGATAAACCGCG‑CGCACCCCCCGTTCAGCGGCACGGCGAGTCAGCGTAGGtt > 1:133115/1‑146 (MQ=255) aTTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACCGTCAGCCCTTCCACCAGACTTAATTCCTGATAAACCGCA‑CGAACCCCCAGTTCAGCGGCACGGCGAGTCAACGTAGCTTCGTCAc < 1:779481/151‑1 (MQ=255) ggTAATCAATCATGCCGTTGCGGCGGGG‑CCCCCCACCCA‑GGCAGAGGGTTTTCGCCCCTGTCAGCCCTTCCACCCGACTTTACTCCCGATAAAACCCG‑CGCACCCCCCGTTCAGCGGCCCGGCGAGTCAGCGGAGCTTCGTCACCCTcc > 2:188036/1‑149 (MQ=255) tAATCAATCATGCCGTTGCGGCGGGG‑CCACTCACCGA‑GGGAGAGGGTTTTCCCCCCTGTCAGCCCCTCCACCCAACTTAACTCCCCATAAAACCCG‑CGCCCCCCCCGTTCAGCGGCACGGCGGGTCAGCGGAGCTTCGTCACCtctccct > 2:528729/1‑144 (MQ=255) atcaatcaTGCCGTTGCGGCGGGG‑CCCCTCTCCCG‑GGCAGAGGGTTTTCCCCCCTGTCAGCCCTTCCACCAGAATTTACTCCTTATAAAAAGCG‑CGCACCCCCCCGTCACCGGCACAGCGAGGCAGCGGAGCCTCGGCACCCTTCCGTcgc > 1:440677/1‑151 (MQ=255) aatcaTGCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCACG‑CGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGt < 2:838849/150‑1 (MQ=255) aTGCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCAGAGGGTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCCCG‑CGCACCCCCCGTTCAGCGGCCCGGCGAGGCAGCGTAGCTTCGTCACCCTCCAGTCGCGTCTCGc > 1:333854/1‑151 (MQ=255) gCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGcc < 1:325469/150‑1 (MQ=255) gCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCa < 1:835588/151‑1 (MQ=255) ggcggGG‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGat < 1:392235/151‑1 (MQ=255) gggg‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGatat < 1:699433/150‑1 (MQ=255) gggg‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATc < 2:63008/151‑1 (MQ=255) ccACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCg < 2:369648/151‑1 (MQ=255) gACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACAGCTAt < 1:543317/150‑1 (MQ=255) gA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCggg > 1:684658/1‑151 (MQ=255) ggCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGc < 2:4907/150‑1 (MQ=255) gCAGCGGTTGTCCCACACTGTCAGCCCTTCCACCAGACTTAACTCCTGATACACCGCG‑CGCACCCCCAGTTCAGCGGCACGGGGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTGGCCAATCCAGATATCACCGCTATCCGGGCGt < 2:205572/151‑1 (MQ=255) gCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGt < 2:551851/151‑1 (MQ=255) agagGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTc > 1:714439/1‑151 (MQ=255) gagGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCg > 2:645268/1‑151 (MQ=255) ggTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGc < 2:794989/150‑1 (MQ=255) | TTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGG‑CCACTGACCGA‑GGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCG‑CGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGC > NC_000913/2677899‑2678186 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |