Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	366,526	0	T	G	41.5%	117.6 / 16.2	66	T329P (ACC→CCC)	lacI	lactose‑inducible lac operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (6/32);  new base G (27/0);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 9.04e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGC  >  NC_000913/366380‑366675                                                                                                                                                     |                                                                                                                                                       gCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTTGGCGCCCgggggggt                                                                                                                                                    >  1:363818/1‑149 (MQ=255)    tGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑GTC‑TTTGGCGGCCGGGGGGttttt                                                                                                                                                 >  1:1984/1‑151 (MQ=255)    tGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTTGGGGCCAGGGGGGgtttt                                                                                                                                                 >  2:851351/1‑151 (MQ=255)        gTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTttttcttt                                                                                                                                             <  2:837292/151‑1 (MQ=255)          gCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTttttcttttc                                                                                                                                           <  1:956701/151‑1 (MQ=255)          gCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGGTTGG‑GTA‑TTTGGGGCCCGGGGGGGttttcttttt                                                                                                                                           >  2:542675/1‑150 (MQ=255)           ccTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCa                                                                                                                                          <  1:791332/151‑1 (MQ=255)             tAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGAGAGAGGCGGTTTTT‑GTA‑TTTGGGGCCAGGGGGGGTTTTTTTTTTAcc                                                                                                                                        >  1:459049/1‑151 (MQ=255)               aTGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑GTA‑TTTGGGGCCAGGGGGGGTTTTTTTTTTAcc                                                                                                                                        >  1:756987/1‑149 (MQ=255)               aTGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTATTTGGGGGCCGGGGGGGttttttttttacc                                                                                                                                         >  1:820013/1‑146 (MQ=255)                 gagtgagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTG‑GTA‑TTTGGGGCCAGGGGGGGttttttttttccca                                                                                                                                       >  1:107527/1‑143 (MQ=255)                    tgagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGATTGT‑TCA‑TTTGGCGGCCGGGCGGTTTTTCTTTtcatc‑a                                                                                                                                      >  1:546175/1‑145 (MQ=255)                     gagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTgaga                                                                                                                                <  2:93974/150‑1 (MQ=255)                      agCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCCTTTCACC‑AGTGAGACg                                                                                                                              <  2:530015/151‑1 (MQ=255)                        cTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑GTG‑TTTGGGGCCAGGGGGGGTTTTTTTTTTCCC‑AGGGAGACggg                                                                                                                            >  2:780416/1‑151 (MQ=255)                           aCTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCaa                                                                                                                         <  2:746789/151‑1 (MQ=255)                            cTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTG‑GTA‑TTTGGGGCCCGGGGGGGTTTTTTTTTTCCC‑CGTGGGACggg                                                                                                                            >  2:608237/1‑147 (MQ=255)                                    aaTTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGAtt                                                                                                                <  2:404274/151‑1 (MQ=255)                                    aaTTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGAt                                                                                                                 <  2:782388/150‑1 (MQ=255)                                      ttgcgttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGCTTGC‑GTA‑TTGGGGGCCAGGGGGGGTTTTCTTTTTAAC‑AGTGAGAAGGGCAAAAGCTGAtt                                                                                                                >  2:643540/1‑149 (MQ=255)                                         cgttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGATTGT‑GGG‑TTGGGGGCCCGGGGGGGTTTTTTTTTCCCCAAGTG‑GAGGGGCGAAAGCTGaagttcc                                                                                                            >  2:452517/1‑144 (MQ=255)                                              cgcTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCAcc                                                                                                      <  1:33670/151‑1 (MQ=255)                                               gcTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑GGA‑TTTGGCGCCCGGGGGGGTTTTTTTTTTACC‑ACGGAGACGGGCAAAAACTGCTTGCCCCTCAccc                                                                                                     >  2:163490/1‑150 (MQ=255)                                                 tCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTG‑GTC‑TTTGGGGCCCGGGGGGGTTTTCTTTTTACC‑AGTGAGACGGGGAACAGCTGAATGCCCTTCACCGcc                                                                                                   >  2:620438/1‑151 (MQ=255)                                                  cACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGAA‑TTGGGGCCCGGGGGGGTTTTTCTTTTTA‑C‑ACAGAGAAGGGCAAAAGCTGGTTTGCCCTCACcccc                                                                                                   >  2:9604/1‑150 (MQ=255)                                                  cACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GCA‑ATTGGCGCCAGGGGGGGTTTTTTTTTCCCC‑AGTGGGACGGGGAACAGCCGATTGCCCCTCACCGcc                                                                                                   >  2:86576/1‑150 (MQ=255)                                                    cTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GGA‑TTGGGCGCCCGGGGGGGTTTTTTTTCCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGcc                                                                                                   >  2:533576/1‑148 (MQ=255)                                                        ccGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑GTA‑TTTGGGGCCCGGGGGGGTTTTTTTTTTTCC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCCGGccc                                                                                             >  2:779163/1‑150 (MQ=255)                                                        ccGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GGG‑TTTGGCGCCCGGGGGGGTTTTTTTTTTACC‑CGTGAGACGGGCAACAGCTGATTGCCCTTCACCCCCCGGccc                                                                                             >  2:531060/1‑150 (MQ=255)                                                           cTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑GGG‑TTTGGGGCCAGGGGGGGTTTTTTTTTTACC‑AGTGGGACGGGCAACAACCGGTTTCCCTTTCCCCCCTGGccc                                                                                             >  2:567391/1‑147 (MQ=255)                                                            tttCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTC‑GTA‑TTTGGGGCCCGGGGGGGTTTTTTTTTTACC‑AGTGAGAAGGGCAACAACTGATTGCCCtttccccccctgccc                                                                                             >  1:907309/1‑133 (MQ=255)                                                            tttCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGG‑GTA‑TTTGGGGCCCGGGGGGGTTTTCTTTTCACC‑CGGGAGACGGGGAAAAGCTGATTGCCCTTCCCCCCCTCGGCCTggg                                                                                         >  1:891705/1‑148 (MQ=255)                                                               ccAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGagagag                                                                                     <  1:675351/151‑1 (MQ=255)                                                                    cGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTgca                                                                                <  2:103801/151‑1 (MQ=255)                                                                           ccTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCg                                                                         <  1:21713/151‑1 (MQ=255)                                                                              gTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCgg                                                                        <  1:197341/149‑1 (MQ=255)                                                                                cGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGT‑GGA‑TTGGGCGCCAGGGGGGTTTTTTTTTTTACC‑AGCGGTACGGGCAACAGCTGTTTTGCCTTCACCGCCTGGGCCTGAGGGAGTTGGCGCAAGGGGttc                                                                     >  2:372557/1‑148 (MQ=255)                                                                                 gTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCAc                                                                   <  2:749940/151‑1 (MQ=255)                                                                                   gCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCCAC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGc                                                                 <  2:345056/151‑1 (MQ=255)                                                                                   gCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGc                                                                 <  1:847288/151‑1 (MQ=255)                                                                                          gCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGttt                                                           <  1:923016/150‑1 (MQ=255)                                                                                          gCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGttt                                                           <  1:512331/150‑1 (MQ=255)                                                                                           cATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCCCC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGttt                                                           <  1:357777/149‑1 (MQ=255)                                                                                                 tGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGT‑GTA‑TTTGGCGCCAGGGGGGGTTTTTTTTTTACC‑AGGGAGACGGGGAAAAGGTGATTGCCCCTCACCCCCTGGCCCTGAGAGAGGTGCAGCAAGCGGGCCCCGCCGGGTTGCCCccgc                                                   >  2:90742/1‑151 (MQ=255)                                                                                                   aaTCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGGGCCAGGGGGGTTTTTTTTTTTACC‑AGTGAGACGGGGAAAAGCTGATTGCCCCTCACCCCCTGGGCCTGAGAGAGTTGCAGCCAACGGGCCACCCTGGGTTGCCCccgc                                                   >  1:454894/1‑149 (MQ=255)                                                                                                   aaTCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTGCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCcagca                                                  <  1:383944/150‑1 (MQ=255)                                                                                                      cGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGc                                               <  2:330742/150‑1 (MQ=255)                                                                                                       ggCCAACGCGCGGGGAGAGGCGGTTTGG‑GTA‑TTTGGGGCCCGGGGGGTTTTTCTTTTTCCC‑AGCGGGACAGGCAACAGCTGATTGCCCTTCACCACCCCGCCCTGAGGGGGTGGCACAAAAGGGGCCACGCTGGGTTGCCCCCGCAGGCg                                              >  2:893612/1‑150 (MQ=255)                                                                                                         ccATCGCGCGGGGAGAGGCGGTTTGC‑GGA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGCCGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCcagc                                                   <  2:546047/143‑1 (MQ=255)                                                                                                           aaCGCGCGGGGAGAGGCGGTTTTT‑GGT‑CTGGGCGCCCGGGGGGGTTTTTTTTTTTCC‑AGGGAGAAGGGCCACAGCTGATTGGCCTTCACCCCCCTGCCCTCAGAGAGTGGCCGCCAGCGGGCCCCGCTGGGTTGTCCCCGCCGGCGgaaa                                          >  2:48962/1‑150 (MQ=255)                                                                                                             cgcgcgGGGAGAGGCGGTTTTC‑GTG‑TTTGGCGCCCGGGGGGGTTTTCTTTTTCCC‑AAGGAGAGGGGGAAAAGCTGAATGCCCTTCACCCCCCCGGCCTGAGAGAGTTGCAGCAAACGGTTCACCCAGGGTTTCCCCAGCAGGCGGAAAAcc                                       >  2:945764/1‑151 (MQ=255)                                                                                                                gcgGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGt                                    <  2:535103/151‑1 (MQ=255)                                                                                                                  ggggAGAGGCGGTTGGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGttg                                  <  2:349113/151‑2 (MQ=255)                                                                                                                        agGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGCtgg                             >  2:717667/1‑150 (MQ=255)                                                                                                                           cGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATggtggt                         >  2:842325/1‑151 (MQ=255)                                                                                                                            ggTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGGGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGtt                        >  2:526158/1‑151 (MQ=255)                                                                                                                             gTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTa                       <  2:452550/151‑1 (MQ=255)                                                                                                                                tGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcg                    >  2:806473/1‑151 (MQ=255)                                                                                                                                 gC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcgg                   >  2:79805/1‑151 (MQ=255)                                                                                                                                 gC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcg                    <  1:431641/150‑1 (MQ=255)                                                                                                                                  c‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcggc                  <  1:643311/151‑1 (MQ=255)                                                                                                                                         tGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGa              <  1:238700/150‑1 (MQ=255)                                                                                                                                          gggCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGat             <  1:973235/150‑1 (MQ=255)                                                                                                                                            gcgcCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGAtata          <  2:648584/151‑1 (MQ=255)                                                                                                                                              gcCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATaaaa         >  2:931854/1‑150 (MQ=255)                                                                                                                                              gcCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAAc        <  1:697332/151‑1 (MQ=255)                                                                                                                                              gcCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAAc        <  2:458936/151‑1 (MQ=255)                                                                                                                                                   ggtggtTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAg   <  1:482413/151‑1 (MQ=255)                                                                                                                                                    gtggtTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGc  <  1:626519/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                       GCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC‑GTA‑TTGGGCGCCAGGGTGGTTTTTCTTTTCACC‑AGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGC  >  NC_000913/366380‑366675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑