| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,873 | 0 | C | T | 41.0% | 32.0 / 18.9 | 39 | S273N (AGC→AAC) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base C (14/9); new base T (0/16); total (14/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.56e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAG > NC_000913/357723‑358009 | gCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGc > 1:673567/1‑151 (MQ=255) tGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTAtt > 1:224101/1‑151 (MQ=255) gTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccgc > 1:442401/1‑151 (MQ=255) gCGTAGCGCATCAGGCAATTCCAGCCGCAGTCCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAcc < 1:201229/150‑1 (MQ=255) tggcgcACCGGGCAATCCCAGCCCCAGACCTGTGTCAGCGGCTACCGGGATTCATTCCCGCCACCAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTCCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgcc < 2:103072/148‑1 (MQ=18) aGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGt > 1:956945/1‑151 (MQ=255) cgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtc > 2:584220/1‑151 (MQ=255) cATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtc > 2:566831/1‑149 (MQ=255) tCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtctctc > 2:362773/1‑151 (MQ=255) tCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtctctc > 2:63936/1‑151 (MQ=255) aaTTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCagt > 1:229798/1‑149 (MQ=255) tcccgcccccAGCCCTGTGCCGGCGGCTCCCGTGTTTCTTTCCCCCCACCACCCGCGCTTTTACCCAACCCCTTGGGAAAAAA‑TGCCTCCGGGGCGCCTTTCTGCCGTTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCagtagt < 2:23232/141‑1 (MQ=255) ccccgCCGCACTCCTGTCTCCCCGTCTCCCGTGATTCTTTCCCCCCAACACCCGCCCATTCACCCACCGCCATGTGAAAAAA‑TGCCTTCGCGGCGGCTGCCGGCAAGTTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTg < 2:375856/146‑1 (MQ=255) cAGCCGCAGCCTTTTGTCAGCGGCTCCCGTGTTTCTTTCCCGCCAACACCCGCGCTTTTATCCACCCCCATGTACAAAAA‑TCCCTTCGCGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCg < 1:394176/151‑1 (MQ=255) cggagccctgcgcgggCGTCTACCGTATTTCATCCCCCCCAACACCCCCTCATTT‑CCCCACCCCCTGTCAAAAAACTGCCCCCGGGGGGGTTGTCGGCCGGCTTTTTCTCCCCCACAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGc < 2:226110/136‑1 (MQ=255) tgtCAGCGGCTCCCGTGATTCATTTCCGCCAACACCCGCGCATTTACCCACCGCCATGTAAAAAAG‑TCCTTGCGCGGCGTCGGTCTGCCAGTTTTTTTCCCGCCCAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGaaa < 2:715979/149‑1 (MQ=255) gtCGGCGGCTCCCGTTATTCTTTCCCGCCAACACCCGCGTTTTTACCCAACGCCTGGTGCAAAAA‑TGCCTCCGCGGCGGCTGTCGCCCAGTTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAg‑aga < 1:680831/146‑1 (MQ=255) tACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAG‑AGaaaaaaggtttaaac > 2:445125/1‑139 (MQ=25) attcattcCCCCCAACAACCGCGCTTTTTTCCAACCCCATGTGAAAAAA‑TGCTTTCGCGGCGGCTGTCTCCCAGCTTTTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAG‑CTTTAAGCCCGTCa < 1:584036/149‑1 (MQ=255) tCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGTGAGAAGAAAAAT‑CTTTaaagcgcctcctt > 1:379540/1‑140 (MQ=25) ttccccccAACACCCGCGCATTTACCCAACGCCATGTGAAAAAA‑TGCCTTCGGGGGGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAG‑CTTTAAGCCCGTCAtgttg < 1:70352/143‑1 (MQ=255) ttcccccaaacaccCGCGCTTTTACCCACCCCCATGTAAAAAAA‑TGCTTCCGCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAG‑CTTTAAGCCCGTCAtgttg < 2:204062/141‑1 (MQ=255) ttcccGCCAACCCCCGCGCTTTTACCCAACGCCTTGTACAAAAA‑TGCTTCCGCGGCGCTTGTCTCCCAGCTTTTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAG‑CTTTAAGCCCGTCAt < 1:316642/142‑1 (MQ=255) tcccGCCACCACCCGCGCATTTCCCCAACGCCATGTACAAAAA‑GGCCTCCGCGGCGGCTGTCTCCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCCCTCCAGTAGTGGCGGGGCAAG‑AGAAATAG‑CTTTAAGCCCGTc < 2:310314/140‑1 (MQ=255) ccGCAACCACCCGCGCTTTTATCCACCGCCATGTGAAAAAA‑TCCCTCCGCGGCGGTTGTCTGCCAGTTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAG‑CTTTAAGCCCGTCATGTtgtgtg < 1:428019/150‑1 (MQ=255) ccaccaCCCGCGCTTTTACCAAACGCCTTGTGAAAAAA‑TGCCTCCGGGGCGGTTGTCTGCCAGTTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGc < 1:563449/149‑1 (MQ=255) ccaccaCCCCCGCTTTTACCCACCGCCAGGTAAAAAAG‑GGCTTCCGCGGCGGCGGCCGCCCGGTTTTTTTCCCCCCCCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAG‑CTTTAAGCCCGTCATGTtgtgtg < 2:627846/147‑1 (MQ=255) accacccGCCCTTTTACCCAACGCCATGTGAAAAAA‑TCCCTCCGGGGCGGCTGTCTGCCATTTATTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGCAATc < 1:597484/146‑1 (MQ=255) aacaccCGCGCATTTACCCAACGCCATGTGCAAAAA‑GGCCTCCGCGGCGGCTGTCTGCCATTTATTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGCAATc < 1:841172/151‑1 (MQ=255) cgcgCTTTTACCCAACGCCATGTCAAAAAG‑TGCCTTCGCGGCGGTTGTCTGCCACTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGCAATCgctg < 1:718001/149‑1 (MQ=255) aaCGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAG‑AGAAAAAGAGTTTTAACCC‑TCCTTTTTTGTGGGAATCGCTGCTGGtgaaaaagt > 2:433858/1‑141 (MQ=25) ccttgtcaaaaat‑TCCCTGCGGGGCGGCTGTCTCCCACTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGa < 1:455003/140‑1 (MQ=255) gtgCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAAAAA‑CTTTTAACCCGCCCTGTTGTTGGGGGATCGCTGCTGGTAACAAtaggg > 1:169724/1‑139 (MQ=255) aaaaaa‑TGCTCCCGCGGGGGTTCTCTGCCTGTTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGaa < 2:892681/147‑1 (MQ=255) aaaag‑TCCCTCCGCGGCGGCTGTCTGCAGGTTATTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGaa < 2:76547/147‑1 (MQ=255) aaaaa‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAG‑AGAAAAAA‑AATTAAAACCCTCATGTTTTGTGGGGATTGCTGGTTGTGACAAAGTGGGAAGGGGAgggg > 1:475917/1‑148 (MQ=255) gCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AAAAAAAA‑ATTTTATACCCTCATGTTTTGTGGGGATTGCTTCTGGTTGCAAAGTGGGGAGGGAAGGgcggcg > 1:713284/1‑149 (MQ=255) tccgcgGCGGCTGTCTGCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAAt < 1:304353/147‑1 (MQ=255) tcccgggggcttGTCTTCCCGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGTAATAG‑CTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcggcg < 2:953710/135‑1 (MQ=255) cAGCTTTTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAg < 1:71996/140‑1 (MQ=255) | GCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAG‑AGAAATAG‑CTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAG > NC_000913/357723‑358009 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |