Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	650,464	0	A	T	44.1%	81.1 / 29.9	59	R9R (CGT→CGA)	citE	citrate lyase, citryl‑ACP lyase (beta) subunit
Reads supporting (aligned to +/- strand):  ref base A (6/27);  new base T (26/0);  total (32/27)
Fisher's exact test for biased strand distribution p-value = 2.32e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGT  >  NC_000913/650318‑650614                                                                                                                                                   |                                                                                                                                                        aTGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACgtt                                                                                                                                                     <  2:946531/151‑1 (MQ=255) aTGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACgtt                                                                                                                                                     <  2:315668/151‑1 (MQ=255)      gcgggcggTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGc                                                                                                                                                <  1:554810/151‑1 (MQ=255)      gcgggcggTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTCgtttttt                                                                                                                                                 >  2:109623/1‑149 (MQ=255)      gcgggcggTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTCgttgtt                                                                                                                                                  >  1:602703/1‑149 (MQ=255)                  tttttCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGAGCGAGGTTTTTGTTTGTGCGGCGAAGCgg                                                                                                                                      >  1:421224/1‑149 (MQ=255)                   ttttCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGGGGGGTTTTCGTTGTTTTAGCGCAGcag                                                                                                                                      >  1:686012/1‑146 (MQ=255)                   ttttCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTCGTTTTTGCAGCGAAACgg                                                                                                                                      >  1:910405/1‑148 (MQ=255)                   ttttCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTTTTTCAGCGAAACGGaa                                                                                                                                    >  1:972756/1‑150 (MQ=255)                   ttttCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCCCGTTTTTCGGTGTTGGACCGAAGCGGaa                                                                                                                                    >  1:110477/1‑150 (MQ=255)                     ttCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGGTTTTTGTTGTTGCAGCGAAACGGaaa                                                                                                                                   >  1:788639/1‑149 (MQ=255)                     ttCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAAt                                                                                                                                  <  1:156997/150‑1 (MQ=255)                       cACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCa                                                                                                                                <  2:857530/150‑1 (MQ=255)                       cACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGCGGGTTTATGGTTTTTCAGCGAAGCGGGAAACa                                                                                                                                >  2:692046/1‑150 (MQ=255)                            aaTGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGTTTTTCGTTGTTGCAGCGAAGCGGAAATCAtt                                                                                                                              >  1:270628/1‑147 (MQ=255)                            aaTGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTGCAGCGAAGAGGGAAACa                                                                                                                                >  1:560850/1‑145 (MQ=255)                               gCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAAtc                                                                                                                       <  1:843016/151‑1 (MQ=255)                                 tACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTGGCGCGAAGCGGGAAACATTGGGAAtcc                                                                                                                      >  1:441993/1‑150 (MQ=255)                                  aCGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGTTTTTTGTTTGTGGAACGAAACGGAAATCATTTGCaa                                                                                                                         >  1:761444/1‑146 (MQ=255)                                  aCGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAAtcctc                                                                                                                    <  2:602724/151‑1 (MQ=255)                                      aGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTCGTTGTTGCAGCGAAGCGGAAAACATTGGCAATCCTccc                                                                                                                  >  1:530677/1‑149 (MQ=255)                                          ttCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTGGGGCGGAGCGGGAAACAATGGGCAACCTccc                                                                                                                  >  2:166612/1‑145 (MQ=255)                                           tCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGCGGTTTTCGTTGTTGCAGCGAAGCGGAAATCATTTGCAATCCCCCCCTgg                                                                                                              >  2:393797/1‑148 (MQ=255)                                              aGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGAGGTTTTTGTTTTTGCAGCGAAACGGAAAACAATGGCAATCCTCCCATGGGag                                                                                                           >  1:920608/1‑148 (MQ=255)                                                gTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGGTTTACGTTGTTTGGGAGAAACGGGCAACATTTGCAATACTCCCCTGGCCGA‑Gcc                                                                                                      >  2:226788/1‑150 (MQ=255)                                                  cAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCgg                                                                                                    <  1:1052723/150‑1 (MQ=255)                                                   aaaCATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTTGTTGTTGCAGCGAAGCGGAAAACATTGGCAATCCTCCCCTGGCAGA‑GCCggg                                                                                                   >  1:260425/1‑150 (MQ=255)                                                    aaCATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTTGTTGTTGCAGCGAAAAGGGAAACATTTGCAATCCTCCCCTGGCCGA‑GCCggg                                                                                                   >  1:461493/1‑149 (MQ=255)                                                        tCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTTCGGCGAAGAGGGAAAAATTGGGAAACCCCCCCTGGGAGAGGCCgggga                                                                                                 >  2:1113631/1‑146 (MQ=255)                                                         cAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCg                                                                                             <  2:972792/150‑1 (MQ=255)                                                                 cAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGCGTTTTTTGGTGTTTGCGCGGAGAGGAAAAAATTTGGAATCCACCCATGGCAGA‑ACCGGGGTAcccccgg                                                                                         >  1:848206/1‑141 (MQ=255)                                                                       ggTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCcagc                                                                              <  2:990778/151‑1 (MQ=255)                                                                        gTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCcagca                                                                             <  2:867015/151‑1 (MQ=255)                                                                                    gTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTc                                                                 <  2:1070986/151‑1 (MQ=255)                                                                                    gTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTc                                                                 <  2:1085887/151‑1 (MQ=255)                                                                                    gTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTc                                                                 <  2:788663/151‑1 (MQ=255)                                                                                       gCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGt                                                               <  2:173967/150‑1 (MQ=255)                                                                                        cTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGGTTTTCGTTGTCGCAGCGAAGCGGAAAACATTGGGAATCCTCCCCTGGCAGA‑GCCGGGGTACCGCCGGCGCGGGCCAGCAGGGCTTCCCGTc                                                                 >  1:1030538/1‑147 (MQ=255)                                                                                        cTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTGCAGCGAAGCGGAAAAAATTGGCAATCCCCCCCTGGCCGA‑GCCGGGAGACCGCCGGCGCGGGCCCGCAGGGCTTcc                                                                     >  2:50296/1‑143 (MQ=255)                                                                                         tGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGAGGTTTTCGTTTTTTCAGCGGAGCGGAAAACAATTGCAATCCCCCCCTTGCAGA‑GGCGGGGAACCGCCGGGGCGGGCCCGCAGGGGTTcc                                                                     >  2:481958/1‑142 (MQ=255)                                                                                          gACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCAc                                                           <  2:842926/151‑1 (MQ=255)                                                                                           aCCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACg                                                          <  1:244677/151‑1 (MQ=255)                                                                                                cgcgGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTaaaa                                                     <  1:734053/151‑1 (MQ=255)                                                                                                cgcgGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTaaa                                                      <  1:810602/150‑1 (MQ=255)                                                                                                 gcgGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAAt                                                    <  2:202560/151‑1 (MQ=255)                                                                                                              cAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGGTTTTTCAGCGAAACGGGAATCATTGGCAAACCCCCCCTGGCAGA‑GCCGGGGTACCCCTGGCGCGGGGCAGCAGGGCCTCCCGTCGTGCACGGAAAAATCAGTCCAGTg                                         >  2:139279/1‑149 (MQ=255)                                                                                                                 gCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCt                                    <  2:530487/151‑1 (MQ=255)                                                                                                                   acaaacaaCATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCttt                                  <  1:783356/151‑1 (MQ=255)                                                                                                                   acaaacaaCATGCTGCGGCGGGGGGGGGGTTTTCGTTGTTTCAGCGAAACGGGAAACAATTGCAAACCTCCCCTGGCCGA‑GCCGGGGGACCCCCGGGGCGTGGCCGCCGGGCTTCCAGGCGGGCCCGGAAAAAGCAGTcccgggggccct                                   >  2:18558/1‑140 (MQ=255)                                                                                                                     aaacaaCATGCTGCGGCGGGGGCGGGGTTTTTTTTGTTTCCGCGAAAAGGAAAACAATGGGAAACCCCCCCTGGCAGA‑GCCGGGGTACCCCCGGCGCGTGCCCGCAGGGGTTCCAGTCGGGCACGTAAAAAACAGGCCAGAGGGCCctt                                  >  2:478748/1‑149 (MQ=255)                                                                                                                                 gcggcgGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACAtt                      <  2:734528/149‑1 (MQ=255)                                                                                                                                    gcggGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCt                 <  2:685336/151‑1 (MQ=255)                                                                                                                                    gcggGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCt                 >  1:183517/1‑151 (MQ=255)                                                                                                                                      gggTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGt               <  2:761464/151‑1 (MQ=255)                                                                                                                                        gTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTAc             <  1:299804/151‑1 (MQ=255)                                                                                                                                            gAGTTTTACGTTGTCGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTAcgccg         <  2:1030575/151‑1 (MQ=255)                                                                                                                                                 ttACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCAc    >  1:824935/1‑151 (MQ=255)                                                                                                                                                 ttACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCAc    >  1:1092303/1‑151 (MQ=255)                                                                                                                                                  tACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACg   >  1:24143/1‑151 (MQ=255)                                                                                                                                                   aCGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGt  >  2:585674/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                        ATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGA‑GCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCACGT  >  NC_000913/650318‑650614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑