Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,556,207	0	T	C	41.5%	105.7 / 18.4	65	S215G (AGT→GGT)	rtcA	RNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base T (35/3);  new base C (0/27);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 2.80e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCAC  >  NC_000913/3556060‑3556354                                                                                                                                                      |                                                                                                                                                    ccGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTccc                                                                                                                                                  >  2:582007/1‑151 (MQ=255)     aaCCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGcc                                                                                                                                              >  1:940679/1‑151 (MQ=255)       ccaccTCGGCACTGACGCGCTTTTCACGGGCGACAAAAAATCGTTCGGTGATATTTTCCTTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGGC‑GCGCGGCGGTTTATG‑AATATTCTTTTCATGCAGGAAAAAACTCCCCGCCa                                                                                                                                             <  2:251953/150‑1 (MQ=255)            tCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAgtgt                                                                                                                                         >  2:533462/1‑149 (MQ=255)                         gcTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTc                                                                                                                          >  2:145224/1‑151 (MQ=255)                           ttttCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTc                                                                                                                          >  1:23382/1‑149 (MQ=255)                           tttcCACCGACGACAAAAAGGTGTGCGGGGATTTTTCCCCTTCCGACTTCAAGCGAGACGGTATTCCCCGCCCCTGGGCC‑GCGCGGCAGTTTATA‑AATTTTCTTTTCAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCt                                                                                                                           <  2:29154/148‑1 (MQ=255)                               cACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCAGCCATTGTAGCGATTTCGCGCTCAGCa                                                                                                                      >  2:523546/1‑149 (MQ=255)                                 ccgccgAAAAAAATGCGTTGGGGTATTTTTCCCCTTTCGACTTAAAGCGAGCCGGTTTTCCCCGCCCCCTGGCC‑GCGCGGCAGGTTATA‑ATTTTTCTGTCCAGGAAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACa                                                                                                                   <  2:565263/150‑1 (MQ=255)                                 ccGACGACAAAAAAGCGTTCGGTGATATTTTCCCTTTCGCCTCCAAGCGAGACGGTATTCCCCGGCCCCGGGCC‑GCGCGGCGGGTTATG‑AATTTTCTTTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACa                                                                                                                   <  2:844254/150‑1 (MQ=255)                                 ccGACGAAAAAAAGGCGTTCGTGGATATTTTCACTTTGGTCTTCAAGAGAAACGTTATTCCCCGCCCCCTGGTC‑GCTGGGCTGGTTATG‑AATATTCTTTTCATGGAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCaa                                                                                                                     <  1:106845/148‑1 (MQ=255)                                     caaaaaaaaaGCGTTCGGTATTTTTTCCACTTCCGCCTTCAAGGGAGACGTTATTCCCCGCCCCCGGGCC‑GCGCGGCGGTTTATA‑AATTTTCTGTTCAGGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgc                                                                                                               <  2:284694/146‑1 (MQ=255)                                        aaaaaagGCGTGCGGTGATTTTTCCCCTTTCGACTACAAGCGAGACGGTATTCCCCGGCCCCTGGCC‑GCGCGGCAGTTTATA‑AATTTTCTGTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCgg                                                                                                           <  2:311590/150‑1 (MQ=255)                                          aaaaaGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGc                                                                                                          >  2:896452/1‑150 (MQ=255)                                                  ttCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCt                                                                                                 >  2:830584/1‑151 (MQ=255)                                                   tCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCt                                                                                                 >  1:787506/1‑150 (MQ=255)                                                    cGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCTCGCTCAGCAACATGGCGCGGCACACCAGCTa                                                                                                >  1:994916/1‑150 (MQ=255)                                                        gATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAAt                                                                                              >  2:941930/1‑148 (MQ=255)                                                          tatTTTCTCTTTCGACTACAGGGGAGACGGTATTCCCCGGCCCCTGGCC‑GCGCGGCAGTTTATA‑ATTTTTCTGTTCATGCAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGa                                                                                           <  2:744480/149‑1 (MQ=255)                                                           atTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACt                                                                                        >  1:685537/1‑151 (MQ=255)                                                            ttttCCCTTTCGACTTAAAGCGAGACGGTATTCCCCGGCCCCGGGCC‑GCGCGGCAGGTTATA‑AATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc                                                                                         <  1:729420/149‑1 (MQ=255)                                                              tcctcTTTCGACTTCAAGCGAGGCGGTATTCCCCGGCCCCGGGTC‑GCGCGGCAGTTTATA‑AATATTCTGTTCATGCGGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtc                                                                                      <  1:565326/146‑1 (MQ=255)                                                                 aCTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTcc                                                                                   >  2:361509/1‑150 (MQ=255)                                                                  cTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCAc                                                                                 >  2:336906/1‑151 (MQ=255)                                                                    ttCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGc                                                                               >  2:505057/1‑151 (MQ=255)                                                                      cgccTTCAAGCGAGACGGTATTCCCCGCCCCCTGGTC‑GCGCGGCAGTTTATA‑AATTTTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCAt                                                                             <  1:606367/148‑1 (MQ=255)                                                                       gACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATc                                                                            >  1:739644/1‑151 (MQ=255)                                                                          ttCAAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGc                                                                         >  1:117380/1‑151 (MQ=255)                                                                             aaGGGAGACGTTATTCCCCGGCCCCTGGTC‑GCGCGGCAGTTTATG‑AATATTCTGTTCATGCAGGGAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTg                                                                          <  1:612430/147‑1 (MQ=255)                                                                             aaGCGAGCCGTTATTCCCCGGCCCTGGGCC‑GCGCGGCGGTTTATG‑AATATTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGc                                                                         <  1:803708/148‑1 (MQ=255)                                                                              aGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcaca                                                                      >  1:858035/1‑150 (MQ=255)                                                                                   gccGGTATT‑CCCGGGCCCTGGGCGGCGCGGAAGGTTATA‑AAATTTTTTTACATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt                                                                  <  1:498611/147‑1 (MQ=255)                                                                                   gaCGTTTTTCCCCGGCCCCTGGCC‑GCGCGGCAGTTTATA‑AATTTTCTTTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGtt                                                                 <  1:979622/150‑1 (MQ=255)                                                                                      gttttccccgggCCCCTGGTC‑GCGCGGCAGTTTATA‑AATATTTTTTTCATGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGtt                                                                 <  2:403105/137‑1 (MQ=255)                                                                                      gttATTCCCCGGCCCCGGGCC‑GCGCGGCAGTTTAGA‑AATATTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGtt                                                                 <  1:788307/145‑1 (MQ=255)                                                                                      ggTATTCCCCGCCCCGTGCTC‑GCGCGGCGGTTTAAA‑AATTTTCTGTTAAGGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTcct                                                              <  2:113247/150‑2 (MQ=255)                                                                                         aTTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCgcgc                                                          >  2:930752/1‑151 (MQ=255)                                                                                          ttccccGGCCCCGGGCC‑GCGGGGC‑GGTTAAGAAATTTTCTTTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcaca                                                                      <  2:945509/135‑1 (MQ=255)                                                                                           tACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTc                                                        >  1:105556/1‑151 (MQ=255)                                                                                                gcccccGGGCC‑GCGCCGCAGTTTATG‑AATATCCTTTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCa                                                    <  2:166278/148‑1 (MQ=255)                                                                                                  ccccTGGTC‑GCGGGGCAGTTTATA‑AATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                                  <  1:737914/150‑1 (MQ=255)                                                                                                    ccTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTg                                               >  1:700070/1‑151 (MQ=255)                                                                                                    ccTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTg                                               >  2:670521/1‑151 (MQ=255)                                                                                                      tGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGc                                              >  1:29266/1‑150 (MQ=255)                                                                                                       ggTC‑GCGCGGCAGGTTATG‑AATATTTTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                             >  2:779651/1‑150 (MQ=255)                                                                                                              gcgGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGtt                                      >  2:138772/1‑151 (MQ=255)                                                                                                              gcgGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGtt                                      >  1:40923/1‑151 (MQ=255)                                                                                                               cgGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgtgt                                       >  1:817992/1‑149 (MQ=255)                                                                                                                    ggTTATA‑AATTTTCTGTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAc                                  <  1:681823/149‑1 (MQ=255)                                                                                                                      ttATA‑AATTTTCTGTCAATGCAGAAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                               <  2:684661/150‑1 (MQ=255)                                                                                                                       tATG‑AATTTTCTTTCCATACAGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATg                              <  1:1008618/150‑1 (MQ=255)                                                                                                                            aaTATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACg                         >  2:124763/1‑151 (MQ=255)                                                                                                                                tttttttCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACggg                       <  2:648393/144‑1 (MQ=255)                                                                                                                                ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCg                     >  2:368110/1‑151 (MQ=255)                                                                                                                                 tCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCg                     >  1:277758/1‑150 (MQ=255)                                                                                                                                     ttCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACt                >  2:1014205/1‑151 (MQ=255)                                                                                                                                       catgaggagAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCga                    <  2:730154/137‑1 (MQ=255)                                                                                                                                       cATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTc              >  2:411076/1‑151 (MQ=255)                                                                                                                                       cATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACt                <  2:376665/149‑1 (MQ=255)                                                                                                                                          gaaGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGAc                 <  1:523687/143‑1 (MQ=255)                                                                                                                                             gggAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGc        >  1:262166/1‑151 (MQ=255)                                                                                                                                             gggAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGc        >  1:121884/1‑151 (MQ=255)                                                                                                                                             ggaaaaacCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTg         <  2:857821/147‑1 (MQ=255)                                                                                                                                             ggaaaaacCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGc        <  2:330892/148‑1 (MQ=255)                                                                                                                                                    aCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCcaccac  >  1:416272/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                    CCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTC‑GCGCGGCAGGTTATG‑AATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCACCAC  >  NC_000913/3556060‑3556354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑