Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,413,735	0	A	T	43.3%	130.2 / 61.2	90	Y2* (TAT→TAA)	ydaC	DUF1187 family protein, Rac prophage; putative double‑strand break reduction protein
Reads supporting (aligned to +/- strand):  ref base A (2/49);  new base T (39/0);  total (41/49)
Fisher's exact test for biased strand distribution p-value = 1.75e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGG  >  NC_000913/1413592‑1413885                                                                                                                                                 |                                                                                                                                                             tttACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATg                                                                                                                                                        <  1:1049261/150‑1 (MQ=255)   tACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTAAATGaa                                                                                                                                                      >  1:446328/1‑150 (MQ=255)    aCTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAac                                                                                                                                                     <  1:552098/150‑1 (MQ=255)        tCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCt                                                                                                                                                <  1:614687/150‑1 (MQ=255)         cTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑TATTTTTTATATTaaaaacccc                                                                                                                                               >  2:51705/1‑144 (MQ=255)         cTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGC‑AAATTTTTATATGAACA‑CCTcc                                                                                                                                              >  1:985852/1‑151 (MQ=255)            ctctctGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTATATGAAAA‑cccc                                                                                                                                               >  2:593767/1‑145 (MQ=255)             tctctGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTTCATGAACA‑CCCCCatt                                                                                                                                           >  2:1334838/1‑150 (MQ=255)               tctGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTAAATTAAAA‑Ccccccctttt                                                                                                                                        >  1:539891/1‑145 (MQ=255)               tctGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AAATTTTTAAAAGAAAA‑CCCCCctta‑tt                                                                                                                                       >  1:586099/1‑151 (MQ=255)                ctGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTTAATGAACA‑CCCCCctta‑tt                                                                                                                                       >  1:1355200/1‑150 (MQ=255)                ctGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCatta                                                                                                                                          <  2:1364635/148‑1 (MQ=255)                  ggAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTTTATTAAAA‑CCCCCctta‑tta‑tt                                                                                                                                   >  2:1347397/1‑151 (MQ=255)                  ggAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTAAATTAACA‑ACTCCctta‑ttt‑tt                                                                                                                                   >  1:672599/1‑151 (MQ=255)                  ggAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCatta‑tta‑t                                                                                                                                    <  2:402192/150‑1 (MQ=255)                  ggAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCatta‑tt                                                                                                                                       <  2:832436/148‑1 (MQ=255)                       cGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑TAATTTTTTCATTAATA‑Ccccccctttttt                                                                                                                                      >  2:561736/1‑137 (MQ=255)                       cGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTTCATGAACA‑CCCCCCCTT‑TTA‑ttt                                                                                                                                  >  1:962519/1‑147 (MQ=255)                        gCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGt                                                                                                                             <  1:470238/151‑1 (MQ=255)                         ccTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTAAATGAAAA‑ACCCCCTTT‑TTT‑TTTTCAGT‑g                                                                                                                           >  1:1002538/1‑151 (MQ=255)                         ccTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑g                                                                                                                           <  2:468047/151‑1 (MQ=255)                         ccTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑g                                                                                                                           <  1:45300/151‑1 (MQ=255)                          cTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTACATGAAAA‑ACTCCCTTA‑TTA‑TTTTCAGT‑gg                                                                                                                          >  1:1273937/1‑151 (MQ=255)                          cTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTAAATGAAAA‑CCCCCCTTT‑TTT‑TTTTCTGT‑gg                                                                                                                          >  1:1227904/1‑151 (MQ=255)                             cttcttTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGtt                                                                                                                        <  2:148665/150‑1 (MQ=255)                               tcttTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTTGATTACAA‑CCTCCCTTA‑TTT‑TTTTTCGT‑GGTTc                                                                                                                       >  2:510793/1‑149 (MQ=255)                               tcttTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTc                                                                                                                       <  1:1209295/149‑1 (MQ=255)                                  tttttACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTTAATGAAAA‑CCCCCATTT‑TTT‑TTTTCCGT‑GGGTTGtttt                                                                                                                  >  1:1199113/1‑150 (MQ=255)                                  tttttACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTACAATAATA‑CCCCCCTTT‑TTT‑TTTTCAGT‑GGttgttttt                                                                                                                  >  1:109674/1‑145 (MQ=255)                                  tttttACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGttt                                                                                                                   <  1:403066/150‑1 (MQ=255)                                  tttttACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGttt                                                                                                                   <  2:925349/150‑1 (MQ=255)                                     ttACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTAtt                                                                                                                <  1:668763/150‑1 (MQ=255)                                      tACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTTAATTAAAA‑CCTCCATTA‑TTT‑TTTTCCTT‑GGGTCGttttttt                                                                                                               >  2:961810/1‑149 (MQ=255)                                        ccTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCAt                                                                                                            <  1:387471/151‑1 (MQ=255)                                        ccTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCAt                                                                                                            <  2:1132496/151‑1 (MQ=255)                                        ccTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCAt                                                                                                            <  2:331448/151‑1 (MQ=255)                                        ccTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AAATTTTTTCATTAACA‑CCCCCCTTA‑TTT‑TTTTCCGT‑GGTTCGttttttt                                                                                                               >  1:1027670/1‑147 (MQ=255)                                          tGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGCAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTAAATGAAAA‑ACCCCCTTT‑TTT‑TTTTCAGT‑GGGTCGTTTAtt                                                                                                                >  1:3107/1‑145 (MQ=255)                                           gagaGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCtt                                                                                                         <  2:153804/151‑1 (MQ=255)                                            agagCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTATATTAAAA‑CCCCCCTTA‑TTT‑TTTTTTTT‑GGTTTGTTTTTTTCATCttt                                                                                                        >  2:515865/1‑151 (MQ=255)                                            agagCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCttt                                                                                                        <  1:788746/151‑1 (MQ=255)                                             gagCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTc                                                                                                       <  2:351995/151‑1 (MQ=255)                                                cATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTACAATTAAA‑CCCCCCATA‑TTATTTTCCATT‑GG‑TCGTTTATTTCATCtttt                                                                                                       >  1:144301/1‑147 (MQ=255)                                                 aTTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGt                                                                                                   <  2:687153/151‑1 (MQ=255)                                                    ttttCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGc                                                                                                 <  1:1107194/150‑1 (MQ=255)                                                      ttCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGctt                                                                                               <  1:1282206/150‑1 (MQ=255)                                                        cGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTTAAAGAAAA‑CCCCCCATT‑TTA‑TTTTCAAT‑GGGTTGTTTTTTTCCTCtttttggtggttttt                                                                                            >  2:1009254/1‑139 (MQ=255)                                                        cGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGcttctt                                                                                            <  2:971498/151‑1 (MQ=255)                                                          cATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCtttt                                                                                          <  1:7870/151‑1 (MQ=255)                                                             tCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AAATTTTTATATTAAAACCCCCCCTTT‑TTT‑TTTCCGTG‑GGTTGGTTTTTTCCTTTTTTTG‑GGGCttttttttt                                                                                        >  2:448535/1‑149 (MQ=255)                                                                gATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCa                                                                                       <  2:550306/148‑1 (MQ=255)                                                                gATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTACGTTTATTCCATCTTTCGAGTGCTTCTTTTTCa                                                                                       <  1:1205258/148‑1 (MQ=255)                                                                 aTTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑TATTTTTTATATTAACA‑CCACCCTTATTTT‑TTTCCACG‑GGTT‑GTTTTTTTTCTCTTTTGAGTGGttttttttttctt                                                                                    >  1:1227879/1‑145 (MQ=255)                                                                    tCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTcca                                                                                <  1:852272/150‑1 (MQ=255)                                                                         aGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTTCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑a                                                                          <  1:1083835/150‑1 (MQ=255)                                                                          gTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AAc                                                                        <  2:159304/151‑1 (MQ=255)                                                                          gTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AAc                                                                        <  1:1222927/151‑1 (MQ=255)                                                                           tttAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTTAAATAAAA‑CCCCCCTTT‑TTT‑TTTTCAGT‑GGGTTGTTTTTTTTATCTTTCTTGTGCTTTTTTTTTTC‑TTCCACCAT‑aa                                                                         >  2:704903/1‑149 (MQ=255)                                                                            ttAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTTAATGAAGA‑CCCCCCTTA‑TTA‑TTTTCAGG‑GGGTCGTTTTTTTCCTTTTTTTAGTGGTTCTTTTTTTC‑TTTCTCCAT‑aaac                                                                       >  1:383524/1‑148 (MQ=255)                                                                            ttAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AAcc                                                                       <  1:254795/150‑1 (MQ=255)                                                                               gATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGtt                                                                   <  2:820435/151‑1 (MQ=255)                                                                                aTTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑TATTTTTTAAATGTAAA‑CCCCCACTT‑TTA‑TTTTCATG‑TGTTTGTTTTTTTCATTTTTTTGGTGGTTTTTTTTTAC‑TTtcacccctaaccg                                                                      >  2:1399494/1‑140 (MQ=255)                                                                                 ttttGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AAATTTTTACATTAAAA‑CCTCCCTTA‑TTT‑TTTTCAGT‑GGGTTGTTTTTTTCATCTTTTTAGGGGTTTTTTTTTAC‑TTCCACCCT‑AAcc                                                                       >  1:957737/1‑145 (MQ=255)                                                                                  tttGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCg                                                                 <  2:962498/150‑1 (MQ=255)                                                                                  tttGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCg                                                                 <  1:1375698/150‑1 (MQ=255)                                                                                    tGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑TATTTTTTTAAATAAAA‑CCCCCATTA‑TTT‑TTTTCAGT‑GGGTTGTTTATTTCATCTTTCTTGTGCTTTTTTTTTTC‑TTCTACCATAAACCGGTTgggg                                                               >  2:711805/1‑151 (MQ=255)                                                                                    tGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTAAATTAACA‑CCCCCCTTT‑TTT‑TTTTCCGT‑GGGTTGTTTTTTTCATCTTTTGCGGGGTTCTTTTTTAC‑TTTCCCCCT‑AAACCGGTCgggg                                                              >  1:130154/1‑150 (MQ=255)                                                                                      aaTATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTTCATGAAAA‑ACCCCCTTT‑TTT‑TTTTCTGTGGGTTGGTTTTTTCC‑TCTTTTTAGTGGTTCTTTTTTTC‑TTTCCCCCT‑AACCGGGTCTGGGtt                                                            >  1:1393570/1‑151 (MQ=255)                                                                                       atatCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTc                                                           <  1:340135/151‑1 (MQ=255)                                                                                       atatCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGA‑TATTTTTTATATTTTAA‑CCCCCCATT‑TTA‑TTTTTAAT‑GGGTCGTTTTTTTTCTCTTTTTAGTGCTTTTTTTTTTC‑TTTCTCCAT‑AACCgggttg                                                                 >  1:676208/1‑141 (MQ=255)                                                                                         atCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTAAATGAAAA‑CCCCCCTTT‑TTT‑TTTTCAGT‑GGTTTGTTTTTTTCCTCTTTTTAGTGCTTCTTTTTTTC‑TTTCACCCT‑AACCCGGTCGGGGtt                                                            >  1:145121/1‑148 (MQ=255)                                                                                         atCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCg                                                          <  2:980561/150‑1 (MQ=255)                                                                                              gTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTAAATGAACA‑ACTCCCTTA‑TTA‑TTTTCAGTGGGTTGGTTTTTTCC‑TCTTTTTAGGGCTTTTTTTTTTC‑TTTCCCCCT‑AAACCGGTCGGGGGTCGccttc                                                     >  1:1013399/1‑146 (MQ=255)                                                                                                   gTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTaca                                               <  2:472904/151‑1 (MQ=255)                                                                                                      agtagGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAAATTTTTTAAAAG‑ACA‑CCCCCCTTT‑TTA‑TTTTCTTG‑GGGTCGTTTTTTTTATTTTTTTGGTGGTTTTTTTTTACTTTCCCCCTA‑AACCGGTCCGGGTTTC‑CGTCCGTTTCACt                                             >  1:639285/1‑150 (MQ=255)                                                                                                       gtagGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTACACTTc                                           <  2:53509/151‑1 (MQ=255)                                                                                                         agGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTTAATTAAAA‑CCCCCCTTA‑TTT‑TTTTCATT‑GGGTTGGTTTTTTTATATTTTTAGGGCTTTTTTTTTTC‑TTTCACCAC‑AACCCGGTCGGGGTTTGCATTCGTTTCACtt                                            >  2:460328/1‑148 (MQ=255)                                                                                                              tGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTTTACATTAACA‑CCCCCCTTA‑TTT‑TTTTCCGTGGGTTGGTTTTTTCC‑TCTTTTCAGTGCTTCTTTTTTTC‑TTTCACCCT‑AACCCGGTCGGGGTTTGCCTCCCTTTCACTTccc                                         >  1:171395/1‑144 (MQ=255)                                                                                                                ccACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTaa                                  <  2:807536/151‑1 (MQ=255)                                                                                                                 cACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAat                                 <  1:749929/151‑1 (MQ=255)                                                                                                                          tttCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGaa                         <  2:1385259/150‑1 (MQ=255)                                                                                                                            tCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAAt                       <  2:682185/150‑1 (MQ=255)                                                                                                                                  gTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTc                <  1:961831/151‑1 (MQ=255)                                                                                                                                  gTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTc                <  1:1140021/151‑1 (MQ=255)                                                                                                                                    ggCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCa               <  1:651238/150‑1 (MQ=255)                                                                                                                                    ggCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCAc              <  1:593780/151‑1 (MQ=255)                                                                                                                                     gCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCAcc             >  2:629801/1‑151 (MQ=255)                                                                                                                                       ggT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGt           <  1:892192/151‑1 (MQ=255)                                                                                                                                           aaTTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTa         <  1:745119/150‑1 (MQ=255)                                                                                                                                             ttttATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTg      >  1:58496/1‑151 (MQ=255)                                                                                                                                                tataCATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATg   >  2:1224069/1‑151 (MQ=255)                                                                                                                                                 ataCATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATgg  <  1:1108796/151‑1 (MQ=255)                                                                                                                                                 ataCATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATgg  <  2:1393544/151‑1 (MQ=255)                                                                                                                                                 ataCATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATg   <  1:510856/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                             TTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGAGAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGT‑AATTTTATACATGAACA‑CCTCCATTA‑TTA‑TTTCCAGT‑GGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCAC‑TTCCACCAT‑AACCGGTTCGGGATTCGCATCCGTTACACTTCGCTCGGTAATATCCTGAAATGGCTTTCACCGTTACTGATGG  >  NC_000913/1413592‑1413885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑