Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,581	0	A	G	41.9%	139.6 / 31.7	87	noncoding (72/88 nt)	serX	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (9/41);  new base G (36/0);  total (46/41)
Fisher's exact test for biased strand distribution p-value = 5.09e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAA  >  NC_000913/1097437‑1097730                                                                                                                                                  |                                                                                                                                                            aCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑aa                                                                                                                                                      <  4:1248023/151‑1 (MQ=32)          aCGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGTTTG‑AAccccc                                                                                                                                                 >  2:369757/1‑147 (MQ=16)           cGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑Ac                                                                                                                                           <  2:1065663/151‑1 (MQ=39)                   ccAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCg                                                                                                                                    <  1:324347/150‑1 (MQ=255)                    cAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGTTTG‑GACCCCCGAT‑aagccgcc                                                                                                                                     >  2:79087/1‑141 (MQ=11)                             gTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑Ctt                                                                                                                        <  4:1200813/151‑1 (MQ=255)                             gTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑Ct                                                                                                                         <  2:233574/150‑1 (MQ=255)                                 ccAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCa                                                                                                                    <  4:278913/151‑1 (MQ=255)                                  cAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAg                                                                                                                   <  4:588173/151‑1 (MQ=255)                                   aGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGATCG‑AAGCCCCggaacagtgcc                                                                                                                                      >  4:859407/1‑123 (MQ=11)                                    gTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTG‑CGCCCCCCAT‑ACGATTGCGGATTCACA‑CTCTcccg                                                                                                                   >  1:170652/1‑147 (MQ=11)                                    gTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑Ctt                                                                                                                        <  2:751186/144‑1 (MQ=255)                                      tGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGG‑AACCCCC‑CT‑ACGTTTCCGGATAAAAA‑CCTTTCAGGCg                                                                                                                >  4:1325544/1‑150 (MQ=11)                                      tGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCg                                                                                                                <  2:879359/150‑1 (MQ=255)                                           aaCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTcc                                                                                                          <  4:1183238/151‑1 (MQ=255)                                                cGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAg                                                                                                     <  1:263339/151‑1 (MQ=255)                                                 ggTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCG‑AAACCCCCCC‑ACGTTGCCGTATACACA‑ATTTCCAGGCGGGCTCCtt                                                                                                        >  4:1002375/1‑147 (MQ=17)                                                    cGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAAGCGTGCTCCTTCAG‑CCa                                                                                                 <  3:35106/150‑1 (MQ=255)                                                        gCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCG‑AACCCCCCAT‑ACGTTCGCGTATACACA‑CTTTCCCGGCGTGCTCCtttaccctc                                                                                                 >  3:11800/1‑140 (MQ=18)                                                        gCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑Cgg                                                                                           <  4:1123663/151‑1 (MQ=255)                                                          ttCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTG‑AACCCCCCCT‑AACATGCCGTATACAAACCTTTCCA‑GCGGGCTCCTTcctcccccccgg                                                                                            >  1:206528/1‑139 (MQ=14)                                                           tctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ac                                                                                        <  4:215519/150‑1 (MQ=255)                                                             tcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCG‑AACCCCCCCT‑ACCGTTCCGTATACAAA‑CTTTCCAGGCGGGCTCCTTCAG‑CCCCC‑CGG‑aca                                                                                       >  2:906815/1‑149 (MQ=17)                                                             tcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTG‑GACCCCCCCT‑ACGTGGGCGTATACACA‑CATTCCAGGCGGGGTCCTTCAG‑CCCCT‑CCG‑ACAcc                                                                                     >  2:1513028/1‑151 (MQ=17)                                                              cATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTTA‑AGAGGGGGTTCGTAACCCCCACA‑AAGTT‑CCGTAACCACA‑CCTTCCACTCGTTCTTCTTC‑G‑CCAccgcaggcacc                                                                                      >  4:1092496/1‑140 (MQ=2)                                                              cATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑aca                                                                                       <  2:631788/148‑1 (MQ=255)                                                              cATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGA‑GGGGGGGAGTCG‑CAACCCCCAC‑AACCTGCCCTATACACA‑CTCTTCACGCCTGCTCCCTCTG‑CCCCC‑CGG‑GCAccc                                                                                    >  2:949126/1‑150 (MQ=9)                                                               aTCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTc                                                                                   <  1:418731/151‑1 (MQ=255)                                                                  cccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGG‑GGGGGGGGGTTG‑AAACCCCGAG‑AAAATCGCGGATAAAAA‑ACCTCCAGGGGGGCGCCCTCAG‑CCACA‑CGG‑ACAACACAcc                                                                                >  2:667005/1‑151 (MQ=2)                                                                    cccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGG‑AACCCCCCCT‑‑CGTTTCCGGCTAACCA‑CTTTCCCAGCGTGGTCCTTCAA‑CCCCC‑CCG‑ACAccccccc                                                                                >  4:1639144/1‑144 (MQ=2)                                                                      cGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTTG‑AACCCCCCAT‑ACCTTGCCGTATAAACA‑CCTTCCAGGCGTGCTCCTTCCG‑CCACC‑CGG‑GCACCCCACCaaa                                                                             >  2:333635/1‑150 (MQ=14)                                                                      cGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGA‑GGGGGGGGGTTG‑AACCCCCCAT‑ACGGTGCCGGATAAACA‑CCTTTCAGGCGGGGTCCTTCAG‑CCACC‑CCG‑GCCCCCCACCaaa                                                                             >  2:965872/1‑150 (MQ=14)                                                                       ggTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAAtt                                                                           <  2:631768/151‑1 (MQ=255)                                                                       ggTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑AGGGGGGGTTTG‑GACCCCCCCT‑ACGTTGCCGTATAAACA‑CTTTCCAGGCGGGCTCCTTTCG‑CCACC‑CGG‑GCACCCCCCCaaa                                                                             >  2:102991/1‑149 (MQ=14)                                                                         tgtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGCGA‑GGGGGGGATTCG‑AACCCCCCCT‑AAGTTGCCGGATAAACA‑CTTTTCAGGCGGGCTCCTTCAG‑CCACC‑CGG‑ACACCCCACCAAATTGt                                                                         >  3:268125/1‑151 (MQ=14)                                                                          gtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCG‑AAACCCCCAC‑ACCTTGCCGGCTACACA‑CTTTCTAAGGGTGCTCCTTCCG‑CCACT‑CCG‑GCAACCCAccccatt                                                                           >  2:1414360/1‑143 (MQ=14)                                                                          gtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTc                                                                        <  4:447568/151‑1 (MQ=255)                                                                           tgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTG‑GACCCCCCCT‑ACGTTGCCGTATACACA‑CTTTTCCGGCGGGCTCCTTCAG‑CCACC‑CCG‑ACACCCCCCCaaaat                                                                           >  4:1243439/1‑145 (MQ=14)                                                                             tgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGGTTG‑TAACCCCGAA‑ACCCTGGCGGATTAACA‑CCTTCCAAGCGGGGTCCTTCCGCCCCCT‑GGG‑CAACCTCCCCAAAttttggt                                                                      >  2:1349818/1‑146 (MQ=2)                                                                             tgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCG‑AACCCCCCAT‑ACGTTTCCGTATACACA‑CTTTCCACGCGGGCTCCTTCAG‑CCACC‑CCG‑ACACCCCACCAAATTTTCGtt                                                                     >  4:44375/1‑151 (MQ=14)                                                                              gCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCG‑GAACCCCGATAACTTTGCC‑CATAAACA‑CTTTCCAGGCGGGCTCCTTCAG‑CCCCT‑CCG‑ACACCCCCCCaaaattttggt                                                                     >  4:1574868/1‑142 (MQ=14)                                                                              gCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGtt                                                                     <  4:1024136/150‑1 (MQ=255)                                                                               cAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTcc                                                                   <  3:409952/151‑1 (MQ=255)                                                                                     aCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGTTTCT‑AACCCCC‑CT‑ACGTTTTCGTTTTAACC‑ATTTTCCCGCGGGGGCCTTAAG‑CCACT‑CGGCGCACC‑CACCAAAATATTGTTTCTGTCtt                                                             >  4:239813/1‑151 (MQ=2)                                                                                      cGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTT‑AACCCCCCAT‑ACGTTGCCGTATACACA‑CCTTTCCGGCGGGCTCCTTCCG‑CCACC‑CCG‑GCACCCCACCAAAATTTCGTTTCTGTCtt                                                             >  2:507023/1‑150 (MQ=14)                                                                                      cGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTg                                                            <  2:565869/151‑1 (MQ=255)                                                                                       gAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGACAGGGGGGGGATTTG‑AACCCCC‑AT‑TCGTTTCAGGTTAAAAA‑CCTTCCCCGCGGCCGCCTTCAC‑CCCCC‑CCC‑ACACCCCACCCCAATTTCGTTCCTGTCttt                                                            >  2:935944/1‑149 (MQ=2)                                                                                        aaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCt                                                          <  1:413702/151‑1 (MQ=255)                                                                                          aaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTgg                                                        <  4:1105958/151‑1 (MQ=255)                                                                                           aaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGa                                                       <  3:278625/151‑1 (MQ=255)                                                                                            aaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCG‑AACCCCCCAT‑ACGTTTCCGTATACACA‑CTTTTCCAGCGTGCTCCTTCAG‑CCACC‑CGG‑ACCCCTCACCCAATTGTCGTTCCTGtttt                                                             >  3:232183/1‑144 (MQ=14)                                                                                             aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTT‑AACCCCCCCT‑ACGCTTCCGGATAAACA‑CTTTCCAGGGGTGCTCCTTCAG‑CCCCT‑CGG‑ACACCCCACCCAAATTTTGTTCCTGTCTTTCTggga                                                      >  2:903924/1‑148 (MQ=14)                                                                                             aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGA‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAAc                                                     <  1:13969/151‑1 (MQ=255)                                                                                              aaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTG‑GACCCCCCCC‑ATCCTGCCGGATAAACA‑ACTTCCCGGCGGGCTCCTTCCA‑CCACC‑CGG‑ACACCCCCCCCAAATTTTGTTCCTGTCTTTCTggga                                                      >  2:836307/1‑147 (MQ=9)                                                                                              aaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACg                                                    <  4:255268/151‑1 (MQ=255)                                                                                               aGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCG‑AACCCCCGCT‑ACGTTGCCGTATAAAAA‑CTTTTCAGGGGTGCTCCTTCAG‑CCACA‑CCG‑ACACCTCCCCCAACTTTCGTTCCTGTCTTTTTGGGACgg                                                   >  1:381121/1‑151 (MQ=14)                                                                                               aGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACgg                                                   <  4:5230/151‑1 (MQ=255)                                                                                                  ccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGTTTG‑TAACCCCCAC‑ACGGTGGCGTCTTAACA‑CCTTTCAGGCGGGCTCCTTCCG‑CCCCC‑CCG‑ACCCCCCCCCCAATTTTCGTTCCTCTCTTGTTTGTACgg                                                   >  2:1577398/1‑148 (MQ=9)                                                                                                  ccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTG‑AACCCCCCCT‑AAGTTTCCGTATAAACA‑CTTTTCACGCGGGCTCCTTCCG‑CCACT‑CCG‑ACACCCCCCCCAAATTTCGTTCCTGTCTTTCTGGAAAggg                                                  >  4:479477/1‑149 (MQ=14)                                                                                                  ccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCG‑GACCCCCCAC‑ACGTTGCCGTATACACA‑CTTTCCCGGCGGGGTCCTTCAG‑CCACTCCGG‑CCCCCCCCCCCCATT‑TCGTTCCTGTCTTTCTGGGACggggg                                                >  2:879240/1‑149 (MQ=2)                                                                                                  ccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGgcg                                                <  3:170666/151‑1 (MQ=255)                                                                                                      aCTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTa                                             <  4:552709/150‑1 (MQ=255)                                                                                                       cTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTaa                                            <  2:853518/150‑1 (MQ=255)                                                                                                        tttCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCG‑AACCCCCCAT‑ACGTTGCCGTATACACA‑ATTTTCAGGCGTGCTCCTTCCG‑CCACC‑CGG‑GCACCTCACCAAATTGTCGTTCCTGTCTTTCTTGAACGGGCGCTAAtt                                          >  3:115467/1‑151 (MQ=17)                                                                                                        tttCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCG‑AACCCCCCAT‑AAGGTGCCCGATACACA‑CTTTTCCAGCGTGGTCCTTCAG‑CCACC‑CCG‑ACACCCCACCAAATTTTCGTTTCTTTCTTTGTGGGACGGGCCCCAAtt                                          >  4:1223867/1‑151 (MQ=2)                                                                                                         ttCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTG‑GACCCCCCCT‑AAGTTGCCGTATACACA‑CCTTTCCGGCGTGCTCCTTCCG‑CCACC‑CGG‑GCACCTCCCCCAATTTTTGTTCCTTTCTTTCTTGAACGGGCGCTAAttt                                         >  4:226586/1‑151 (MQ=9)                                                                                                          tCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAAttt                                         <  4:1272104/150‑1 (MQ=255)                                                                                                            gTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTG‑GACCCCCCCC‑ACCTTGCCGTTTACACA‑CTTTTCCCGCGGGGTCCCTCCG‑CCCCC‑CCG‑ACACCCCACCCAAATTTCGTTTCTTTCTTGTTGGGACAGGGGCTAAatt                                         >  4:1575970/1‑148 (MQ=2)                                                                                                            gTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGATCG‑AAACCCCCCT‑ACGTTGCCGGATACAAA‑CTTTTCAGGCGTGGTCCTTCCG‑CCACT‑CGG‑ACACCCCCCCCAAATTTCGTTTCTGTCTTTCTGGGACGGGCGCTAAttt                                         >  4:1414251/1‑148 (MQ=14)                                                                                                             tACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGGTCG‑GACCCCCCCC‑AAGATGCCCTATAAAAA‑CCTTCCCGGCGTGCTCCCTTTC‑CCCCC‑CCG‑GCACCTCAACAAATTTTTGGTCCTCTCTTTCTTGGAAAGGCGCCAACTTAggg                                     >  2:311580/1‑151 (MQ=2)                                                                                                                  gCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATc                                <  4:79234/151‑1 (MQ=255)                                                                                                                  gCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATc                                <  3:336337/151‑1 (MQ=255)                                                                                                                    tcttctTTAAATATGGCGGTGA‑GGGGGGGGGTCG‑AACCCCCCCT‑ACGTTGCCGTTTAAACA‑CTTTCCAGGCGGGCTCCTTCAG‑CCACC‑CGG‑ACACCCCACCAAAATTTTGTTCCTGTTTTGCTGGAACGGGCGCCAATTTAGGGAAATCa                               >  3:66304/1‑150 (MQ=17)                                                                                                                     cttcttTAAATATGGCGGTGA‑GGGGGGGGTTCC‑AACCCCCCCT‑ACGTTTTCGCTTAAAAA‑CCTTTCCGGCGTGGCCCTTCAG‑CCACC‑CGG‑GCAACCCACCCAAATTTCGCTTCTTTCTTTCTTGGACGGGCGCCAATTTTGGGgaaaa                                >  4:579432/1‑146 (MQ=2)                                                                                                                          ttAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGAcc                          <  3:175457/149‑1 (MQ=255)                                                                                                                          ttAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGAc                           <  4:251694/148‑1 (MQ=255)                                                                                                                            aaaTATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGa                       <  4:143765/150‑1 (MQ=255)                                                                                                                              atatGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTTCCGGATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTCATTTAGGGAAATCATGACCTgggg                     >  2:214677/1‑150 (MQ=255)                                                                                                                                atGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTca                  <  4:1504830/151‑1 (MQ=255)                                                                                                                                  ggCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaac                <  4:732213/151‑1 (MQ=255)                                                                                                                                  ggCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaac                >  3:412274/1‑151 (MQ=255)                                                                                                                                  ggCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaac                <  4:1118025/151‑1 (MQ=255)                                                                                                                                  ggCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaac                <  3:16164/151‑1 (MQ=255)                                                                                                                                      gTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAAc            >  2:1418206/1‑151 (MQ=255)                                                                                                                                        gA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtt          >  2:463381/1‑151 (MQ=255)                                                                                                                                         a‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACttt         >  4:1124434/1‑151 (MQ=255)                                                                                                                                               gggATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaa    <  2:17157/151‑1 (MQ=255)                                                                                                                                                  aTTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaaaa  >  4:796639/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                            ACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCG‑AACCCCCGAT‑ACGTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAG‑CCACT‑CGG‑ACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAA  >  NC_000913/1097437‑1097730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑