Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	659,942	0	G	A	40.0%	126.0 / 50.3	90	A92V (GCT→GTT)	lipA	lipoate synthase
Reads supporting (aligned to +/- strand):  ref base G (8/46);  new base A (36/0);  total (44/46)
Fisher's exact test for biased strand distribution p-value = 1.02e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCACGGTCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATT  >  NC_000913/659799‑660091                                                                                                                                                 |                                                                                                                                                        catcaCGGTCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAg                                                                                                                                                  <  1:170237/151‑1 (MQ=255)    caCGGTCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAt                                                                                                                                               <  2:149287/151‑1 (MQ=255)    caCGGTCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAt                                                                                                                                               <  3:497869/151‑1 (MQ=255)       agTCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACAAAAAAAGCCGAGGAA‑Ca                                                                                                                                            >  1:500708/2‑150 (MQ=255)           aaCGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAAc                                                                                                                                       <  3:738052/151‑1 (MQ=255)           aaCGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGGAAAAAAAAGCCGCGGGT‑CATaaa                                                                                                                                        >  1:84405/1‑150 (MQ=255)             cGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGt                                                                                                                                     <  3:193444/151‑1 (MQ=255)               gAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTTAAAATAGAGCCGAGGAG‑CATAAACggt                                                                                                                                    >  3:1245651/1‑148 (MQ=255)                   agATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGAAAAAAAAAGCCCAGGGG‑CATaaaagat                                                                                                                                    >  3:964418/2‑142 (MQ=255)                    gATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACCAAAAACGCCGACGGT‑TATaaa                                                                                                                                        >  3:510319/1‑141 (MQ=255)                       aaCCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGcc                                                                                                                            <  1:1223058/150‑1 (MQ=255)                        aCCTCATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACAAAAAACGGCGCGGAA‑CATAAAAAATTCTGTGCCg                                                                                                                           >  3:851478/1‑150 (MQ=255)                        aCCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGt                                                                                                                          <  3:92575/151‑1 (MQ=255)                          cacaTAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTgg                                                                                                                        <  3:1122925/151‑1 (MQ=255)                          cacaTAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACAAAAAACGCCGAGGGT‑CATAAACGTTGCTGTGCCGTgg                                                                                                                        >  2:452284/1‑151 (MQ=255)                           acaTAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACAAAAAACGCCGCGGGT‑CATAAAACATGCTGTGGCgggg                                                                                                                        >  3:745160/1‑150 (MQ=255)                                     cgctcttccgattaaTGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACAAAAAAACCCGCGGGA‑CATAAAAGCTGCTTTGCCGTGGGTGGAGCAtt                                                                                                              >  1:36313/14‑150 (MQ=255)                                      cgcCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACAAAAAAAGCCGCGGGT‑CATAAAACTTGCTGTGCCGGGGGTGTAGCAtt                                                                                                              >  3:791131/1‑149 (MQ=255)                                       gcCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCg                                                                                                           <  1:723043/151‑1 (MQ=255)                                       gcCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACAAAAAAAGCCGCGGGT‑CATAAAACATGCTGTGGCGTGGGTGGAggaattc                                                                                                            >  3:232143/1‑144 (MQ=255)                                         cATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACAAAAAACGCCCGGGGG‑CATAAAAACTTCTGTGCCCTGGGTGGAGGAATCCG‑cc                                                                                                        >  3:1068125/1‑151 (MQ=255)                                          atatCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCa                                                                                                       <  2:112319/151‑1 (MQ=255)                                           tatCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTGAAAAAAACGCCCCGGGT‑TATAAAAGATGCTGTTCCGGGGGTGGAGCacttcccccc                                                                                                       >  2:149626/1‑142 (MQ=255)                                            atCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAg                                                                                                      <  3:619143/150‑1 (MQ=255)                                              cGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGtt                                                                                                   <  3:108269/151‑1 (MQ=255)                                              cGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGAAAAAAAAAGCCGCGGGA‑CATAAAAGACGCTGTGCCGGGGGTGGAGCAATCCC‑CCCgggt                                                                                                   >  2:199251/1‑149 (MQ=255)                                               ggCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTa                                                                                                  <  2:551551/151‑1 (MQ=255)                                               ggCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACAAAAAAAGCCGAGGAG‑CATAAAACTTGCTGTGCCGTGGGTGGAGCATTCCG‑CCCgggt                                                                                                   >  2:499564/1‑148 (MQ=255)                                                  aaTGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAACGCCGAGGGA‑GATAAAACATATTGTGGCCGGGGTGAAACATTTCT‑CCACGTTAggg                                                                                               >  3:1155295/1‑151 (MQ=255)                                                   aTGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGGCAAAAAACGCCCAGGGT‑GATAAACATTTTTGTGGCGGGGGTGGAGAATTTCC‑CCCGGGTAgggg                                                                                              >  1:849693/1‑150 (MQ=255)                                                     ggTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCag                                                                                            <  3:709232/151‑1 (MQ=255)                                                     ggTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCa                                                                                             <  3:943754/150‑1 (MQ=255)                                                     ggTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGAACAAAAAGCGGCGAGGGG‑CATAAAAGGTGCTGTGCCGTGGGTGTAGAAATCCG‑CCCgggttgg                                                                                                >  3:1118062/1‑142 (MQ=255)                                                        cTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGAAAAAAAAAGCCGAGGAT‑CATAAAAGGTGCTGTGCCGGGGGTGGAGGATTTCG‑CCCGGGTAGGGGagg                                                                                           >  3:756382/1‑149 (MQ=255)                                                         tGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACAAAAAACGCCGCGGGG‑CAAAAACAATGCTGTGCCGCGGGTGGAGGATTCCG‑CCCGGGTAGGGGaggggg                                                                                        >  4:417579/1‑151 (MQ=255)                                                          gcgcCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCaggagg                                                                                        <  3:733823/150‑1 (MQ=255)                                                          gcgcCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCagga                                                                                          <  3:712437/148‑1 (MQ=255)                                                          gcgcCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGc                                                                                       <  2:546240/151‑1 (MQ=255)                                                          gcgcCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGGCAAAAAAAGCCCAGGGG‑CATAAAAATTTCTTTGCCGTGGGTGGAGCGTTTCC‑CCCGGGTAGGGGagggggg                                                                                       >  3:1134622/1‑150 (MQ=255)                                                           cgcCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCt                                                                                      <  1:993454/151‑1 (MQ=255)                                                            gcCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACAAATAACGGCCAGGGA‑CATAAAACGTGCTGTGGCGGGGGTGTAGCGATCCG‑CCCGGGTTGGGGaggggg                                                                                        >  3:590301/1‑148 (MQ=255)                                                              cAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGCCAAAAAACGCCGAGGGT‑AATAAAAATTTTTCTGGCGGGGGTGGAGAAGTCCG‑CCCGGGTAGGGGaagagggctc                                                                                    >  1:1309324/1‑146 (MQ=255)                                                               aGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCt                                                                                  <  3:739971/151‑1 (MQ=255)                                                                  ttCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCg                                                                                <  3:937560/150‑1 (MQ=255)                                                                   tCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACAAAAAACGCCGAGGAA‑CATAAAAGTTGCTGTGCCGTGGGTGAAGCATTCCG‑CCCGGGTAGGGGAGGGGGCGTCCTCGcc                                                                              >  3:840294/1‑150 (MQ=255)                                                                    cACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGcag                                                                             <  1:752929/151‑1 (MQ=255)                                                                     aCTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGcaga                                                                            <  4:517120/151‑1 (MQ=255)                                                                        ggTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGcagaca                                                                          <  2:560069/150‑1 (MQ=255)                                                                        ggTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGcagaca                                                                          <  4:163747/150‑1 (MQ=255)                                                                        ggTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGcagaca                                                                          <  1:702832/150‑1 (MQ=255)                                                                           tCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACAAAAAACGCCCCGGGG‑CCTAAACGTTGCTGTGCCGGGGGTGAAGGATTCCG‑CCCGGGTAGGGCAGGGGGGTTCCTCCccgaca                                                                          >  3:1078683/1‑147 (MQ=255)                                                                             aTTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGc                                                                    <  3:389232/151‑1 (MQ=255)                                                                              ttGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACAAAAAAAGCCGGGGAT‑CCAAAACAATGTTGTGCCGCGGGTGTAGCAATTCC‑CCACGGTAGGGGGGGGGGGTTCCCCCCCCACAGaaag                                                                     >  1:736582/1‑147 (MQ=255)                                                                                 gCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGc                                                                <  2:401092/151‑1 (MQ=255)                                                                                   aTCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGAAAAAAAAACCCCGGGAT‑CATAAAAATTGGTGTGCCGGGGGTGGAGGATTTCGCCCAGGTT‑GGGCAGGAGGGTTCCTCCCCGACCGAATGTAGGcc                                                               >  3:894353/1‑150 (MQ=255)                                                                                     cAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAAAAAAGCCGAGGGT‑CATAAAAGGTGCTGTGCCGGGGGTGGAGCATTCCG‑CCCGGGGAGGGGAGGAGGGGTCCTCGCCGACAGAAAGCAGGGCact                                                            >  3:1102493/1‑149 (MQ=255)                                                                                          gCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTgc                                                       <  1:1262596/151‑1 (MQ=255)                                                                                              ccGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGAGAAATAAAGCCGGGGGG‑TATAAAAGGTGCGGTGGCGGGGGTGAAGGAATCCG‑CCCAGGTAGGGGAGGGGGGCTTCTCGCAGACAGAATAGAGGGCAttttttgg                                                      >  4:217058/1‑145 (MQ=255)                                                                                              ccGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACAAAAAACGCCGGGGGG‑CATAAAAATTGCTGTGCCGTGGGTGGAGAATTTCC‑CCAGGGTAGGGGAGGGGGGTTTCTCGCCGACAGAAAGCAGGGCATTTTTTTGCAt                                                   >  3:175443/1‑151 (MQ=255)                                                                                              ccGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACAAAAAACGCCGAGGGG‑CACAAAAATTGCTGTGCCGTGGTTTGAGCATTTCG‑CCAGGGTAGGGGGGGGGGGGTCCTCGCCGGCAGAAAGCAGGGCCtttttttgcc                                                    >  1:1397061/1‑145 (MQ=255)                                                                                               cGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACAAAAAACGCCGAGGGTCCAAAAAAGTGTTTTTGCGGGGGTGGAA‑CAATCCG‑CCCGGGTAGGGGGGGGGGGTTTCCCCCCGACAAAAAGAAGGGCAtttttttgcctt                                                  >  3:1159886/1‑144 (MQ=255)                                                                                                gggCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTg                                                 <  3:319160/151‑1 (MQ=255)                                                                                                gggCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTg                                                 <  4:430851/151‑1 (MQ=255)                                                                                                gggCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTg                                                 <  1:25403/151‑1 (MQ=255)                                                                                                gggCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACAAAAAAGGCCCAGGAG‑CCTAAAAATTTCTTTGGCGTGGGTGTAACAATTCC‑CCAGGGTAGGGGAGGGGGGTTTCTCTCAGACAGAAAGCAAGCCCtttttttgccttt                                                 >  3:556485/1‑143 (MQ=255)                                                                                                 ggCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGc                                                <  1:1148632/151‑1 (MQ=255)                                                                                                  gCGACCGTGGGCAACATCACAGAACGGACAACGGCGGGGGAAAAAAAAGGCCGGGGA‑‑CATAAACGGTTCTGTGGCGGGGGTGAAGGATTTCG‑CCAGGTTAGGGACGGGGGCGTTCTCGCCGACAGAATACAGGGCCTTTTTTTGCAtt                                                  >  3:276262/1‑148 (MQ=255)                                                                                                  gCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCg                                               <  1:1130557/151‑1 (MQ=255)                                                                                                      ccGTGGGCAACATCACAGAACGGACAACGGC‑GGGGATAAAAAAAACCGAGGGA‑CAATAACCTTGCGGTGTCGGGGGTGGAGCAATTCT‑CCCAGTTATGGGAGGAGGGTTTCTCCCCCACAGGAAGTAGGCCAGTTTTTCGCATTGCGGCt                                            >  3:659306/1‑150 (MQ=255)                                                                                                       cGTGGGCAACATCACAGAACGGACAACGGC‑GGGGACAAAAAACGCCGCGGGG‑CATAAAAATTGCTGTGCCGGGGGTGGAGCATTTCG‑CCCGGGTAGGGGGGGGGGGGTCCTCGCCGACAGAAAGCAGGGCATTTTTTTGCATTGCGGCttt                                          >  3:91229/1‑151 (MQ=255)                                                                                                        gTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTg                                         <  3:433256/151‑1 (MQ=255)                                                                                                          gggCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGAt                                       <  1:1281812/151‑1 (MQ=255)                                                                                                           ggCAACATCACAGAACGGACAACGGC‑GGGTAAAAAAAACGCCGCGGAT‑CATAAACAATGCTGTGGCCGGGGTGGAGCATTTCC‑CCCGGGTAGGGGAGGGGGGTTCCTCGCAGACAGAAAGCAGGGCCTTTTTTTGCATTTGGGCttt                                          >  3:19353/1‑147 (MQ=255)                                                                                                                    acaGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATa                             <  3:130549/151‑1 (MQ=255)                                                                                                                      aGAACGGACAACGGC‑GGGTACAAAAAACGCCGGGGAT‑CATAAAAATTGCTGTGCCGGGGGTGGAACAATCCG‑CCAGGGTAGGGGAGGGGGCGTCCTCTCCGACAGAAAGCAGGGCCTTTTTTTGCATTTTGGGGTTTATGCCCCGGATACg                           >  2:84943/1‑151 (MQ=255)                                                                                                                       gAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACgt                          <  3:1163849/151‑1 (MQ=255)                                                                                                                            gACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTaga                     <  1:777022/151‑1 (MQ=255)                                                                                                                             aCAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTagag                    <  3:1306601/151‑1 (MQ=255)                                                                                                                                aCGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTcc                 <  1:406707/151‑1 (MQ=255)                                                                                                                                  ggc‑ggGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGc               >  1:919333/1‑151 (MQ=255)                                                                                                                                         tACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGa           >  3:351190/1‑149 (MQ=255)                                                                                                                                          aCAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAg         <  1:1323735/150‑1 (MQ=255)                                                                                                                                          aCAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAg         <  4:217680/150‑1 (MQ=255)                                                                                                                                            aaaTAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCtt      >  1:208845/1‑151 (MQ=255)                                                                                                                                            aaaTAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCtt      <  1:745050/151‑1 (MQ=255)                                                                                                                                             aaTAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTg     >  3:316343/1‑151 (MQ=255)                                                                                                                                             aaTAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTg     <  1:1117931/151‑1 (MQ=255)                                                                                                                                                aGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGAtt  >  3:833359/1‑151 (MQ=255)                                                                                                                                                aGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGAtt  <  2:204413/151‑1 (MQ=255)                                                                                                                                                aGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGAtt  >  2:254620/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                        CATCACGGTCAACGGAGGTGATAACCACATAACGCAGCGCCATATCGGCAATGGTCTGCGCCAGTTTCACTGGTTCATTGGCATCAGGAGCTACCGGGCGACCGTGGGCAACATCACAGAACGGACAACGGC‑GGGTACAAATAGCGCCGAGGAT‑CATAAACGTTGCTGTGCCGTGGTTGAAGCATTCCG‑CCAGGTTAGGGCAGGAGGCTTCCTCGCAGACAGAATGCAGGCCATTTTTGCGCATTGCGGCTTTGATGCCCTGGATACGTGTAGAGTCCGCTGGAAGCTTGATT  >  NC_000913/659799‑660091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑