Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,434,882	0	T	G	51.4%	85.7 / 118.4	70	Y252S (TAC→TCC)	truA	tRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base T (7/27);  new base G (36/0);  total (43/27)
Fisher's exact test for biased strand distribution p-value = 2.95e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-12

GTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCAT  >  NC_000913/2434736‑2435030                                                                                                                                                    |                                                                                                                                                     gTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCGGGCAGGGGaa                                                                                                                                                    >  2:80121/1‑149 (MQ=255)   ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATTATACCGGGCAGGGGAAtac                                                                                                                                                 >  1:557732/1‑148 (MQ=255)   ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGATCATACCGGGCAGGGGAATcc                                                                                                                                                 >  2:1042503/1‑150 (MQ=255)       atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCGGGCAGGGGAATACACcgc                                                                                                                                            >  1:772817/1‑151 (MQ=255)       atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACcgc                                                                                                                                            <  1:1033785/151‑1 (MQ=255)        tttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCGGTCAGGGGAATCCACcgc                                                                                                                                            >  2:1034010/1‑150 (MQ=255)          tGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAAATCATACCGGTCAGGGGAATcccaccc                                                                                                                                            >  2:328592/1‑143 (MQ=255)            ccTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGATTATACCGGTCAGGGGAAACCACCGCGAcc                                                                                                                                        >  2:991454/1‑150 (MQ=255)               aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATAACCGGCCGGGGAAACCACCGCGACCCGa                                                                                                                                     >  1:1052941/1‑150 (MQ=255)               aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAt                                                                                                                                    <  2:1044610/151‑1 (MQ=255)                 gCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATa                                                                                                                                   <  1:159023/150‑1 (MQ=255)                   cTTGTACTCGTCCTCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGATCATAACGGGCAGGGGAATCCACCCCGACCCGATAc                                                                                                                                  >  2:458587/1‑149 (MQ=255)                      gTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGccc                                                                                                                             <  1:52024/151‑1 (MQ=255)                        aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATTATACCGGGCAGGGGAATACAACCCGACCCGATAccaacc                                                                                                                             >  2:319158/1‑144 (MQ=255)                        aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCGGCCCGGGTAATCCACCGCGACCAGATACAGccc                                                                                                                             >  1:747686/1‑149 (MQ=255)                         cTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCCGGCAGGGGAAACCACCCCGACCCAATACAGCCCtt                                                                                                                           >  1:1144205/1‑150 (MQ=255)                          tCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGGCCTTcc                                                                                                                         >  2:889761/1‑151 (MQ=255)                           cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTcc                                                                                                                         <  2:254154/150‑1 (MQ=255)                           cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTTcc                                                                                                                         >  2:892227/1‑150 (MQ=255)                            gTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATAACGGGCAGGGGAATCCACCCCGACCAGATACAGcccct                                                                                                                           >  1:23276/1‑145 (MQ=255)                               aTCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGATCATAACCGGCAGGGGAATCCCCCCCGACCAGAAACAGCCCTTCCGCttt                                                                                                                    >  1:1037283/1‑151 (MQ=255)                               aTCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑ATTTGGGAGAACCTAACGGTCAGGGGAAACCACCGCGAACAGAAACAGCCCCTCCGCttt                                                                                                                    >  2:1068716/1‑151 (MQ=255)                                  tttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGAACCTACCGGGGAAGGGAATCCACCGCGACCAGATACACGCCTTCCTCctttt                                                                                                                  >  1:596200/1‑149 (MQ=255)                                     cAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCGGGCAGGGGAATCCCCCGCGACCAGATACAACCCTTCCGCTTTTTcc                                                                                                                >  2:635983/1‑149 (MQ=255)                                     cAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCg                                                                                                               <  1:351987/150‑1 (MQ=255)                                      aGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATAACGGTCAGGGGAATCCACCGCGACCAGATACAACCCCTCCGCTTTTTCCGtt                                                                                                             >  2:181699/1‑151 (MQ=255)                                          tGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGATCATACCCGTCAGGGGAATCCACCCCGACCCGATACAACCCTTCCGCTTTTTCCg                                                                                                               >  1:546538/1‑145 (MQ=255)                                           gTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCGGTCAGGGGAATCCACCGCGGCCAGATACAACCCTTCCGCTTTTGCCGTtgctg                                                                                                         >  2:71094/1‑150 (MQ=255)                                                  cGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCACTGGCGG‑TTTTTGAAGATCATACCCGTCAGGGGAATCCACCGCGGCCAGATACAACCCCTCCCCTTTTTCCCTTTGTgccgcc                                                                                                    >  1:490321/1‑148 (MQ=255)                                                   gTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCATACCGGGCAGGGGAATCCACCCCGGCCAGATACAACCCCTCCGCTTTTGCCGTTTCTgcccccc                                                                                                   >  1:142584/1‑147 (MQ=255)                                                   gTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGct                                                                                                <  1:722599/151‑2 (MQ=255)                                                      ggCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTc                                                                                             <  1:1074193/151‑1 (MQ=255)                                                          aCAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGt                                                                                          <  1:1195041/150‑1 (MQ=255)                                                           cAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAAATCATACCGGGCAGGGGAATCCACCGCGACCCGATACAGCCCTTCCGCTTTTTCCGTTTCTGCCCCCAGCGTTCTGTcc                                                                                        >  2:208872/1‑151 (MQ=255)                                                             gCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCtt                                                                                      <  1:994332/151‑1 (MQ=255)                                                              ccTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGCAATAGCGGGCCCATTGGCGG‑GTTTGGTAGATAATAACGGGCAGGGGAATTCACCACGACCAGATACACTCCTTCCTCCTTTTCTGTTGCTGCCGCCCGGGAGCTGTCCttt                                                                                     >  1:200102/1‑151 (MQ=255)                                                                tGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAAG‑TCATACCGGTCAGGGGAAACCACCCCGACCAGAAACAGCCCCTCCGCCTTTGCCGTTGCTGCCGCCCGCGTTTCGTCCttt                                                                                     >  1:367847/1‑149 (MQ=255)                                                                 gAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGTAGATCATACCCGGCAGGGGAATCCACCCCGACCAGATACAGCCCTTCCGCTTTTTCCGTTGCTGCCCCCAGCGTTCTGTCCttt                                                                                     >  1:322879/1‑148 (MQ=255)                                                                 gAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCCTCCGCTTTTGCCGTTGCTGCCGCCCGCGTTCTTTCCTTTgcc                                                                                  >  2:723258/1‑151 (MQ=255)                                                                   aaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGAAGATAACGGTCAAGGGAATTCACCGCGACCAAATACAACCCTTTCGCGTTTTCCGTTGGTGCCGCCAGCGTTCTGTCCTTTg                                                                                    >  2:206190/1‑147 (MQ=255)                                                                    aTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTgccgcc                                                                               <  1:577695/151‑1 (MQ=255)                                                                      gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCa                                                                              <  1:168454/150‑1 (MQ=255)                                                                        tCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTTCCGTTGCTGCCGCCAGCGTTCTGTCCTTTtccgcc                                                                               >  2:539419/1‑147 (MQ=255)                                                                         ccGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGATCATACCCGGCAGGGGAAACCACCCCGACCAGATACAGGCCCTCCCCTTTTTCCGTTTCTGCCGCCCGCGTTCCGTCCTTTGCCGCCAg                                                                             >  1:301928/1‑148 (MQ=255)                                                                          cGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAAc                                                                         <  1:444012/151‑1 (MQ=255)                                                                           gAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAAct                                                                        <  1:964030/151‑1 (MQ=255)                                                                            aGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATAATACCGGTCAGGGGAAAACACCCCGACCAGATAAAGCCCCTCCGCTTTTTCCGGTGCCTCCGCCCGCGGTCTGTCCTTTttcgcccccaa                                                                          >  1:805708/1‑143 (MQ=255)                                                                            aGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAActc                                                                       <  2:1169281/151‑1 (MQ=255)                                                                              cATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTg                                                                     <  1:350670/151‑1 (MQ=255)                                                                               attattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGATCATACCGGTCAGGGGAATCCACCCCGACCAGATACAGCCCTTCCGCTTTTTCCGTTGCTGCCCCCAGCGTTCTGTCCTTTTCCGCCCGCAActct                                                                      >  2:880207/1‑149 (MQ=255)                                                                               attattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGAACAAAACCGTCAGGGTAAACCAACCCGACCCGATAAAGGCCCTCCCCCTTTGCCGTTGCTGCCGCCCGCGTTCTGTCCTTTTTCGCCCGCCActct                                                                      >  2:282036/1‑149 (MQ=255)                                                                                   ttCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTTGGAGATCAAACCCGGCAGGGGAAATCACCGCGACAAGATACAGCCCTTTCCCTTTTTCCTGTGCTGCCGCCCGCGCTCTTTCCTTTTCCCCCCGCCACTATggt                                                                   >  1:136147/1‑146 (MQ=255)                                                                                     ctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGGAGATCATACCGGTCCGGGGAAACCAACCCGACCAGATAAAGCCCTTCCCCTTTTTCCGTTGCTGCCGCCAGCGTTCTGTCCTTTTCCGCCCGCAACTCTGCTATcc                                                               >  2:1057282/1‑150 (MQ=255)                                                                                       cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATcc                                                               <  2:812272/148‑1 (MQ=255)                                                                                       cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATcc                                                               <  1:631614/148‑1 (MQ=255)                                                                                       cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCa                                                              <  1:486125/149‑1 (MQ=255)                                                                                             tCCGCCAGAAATAGCGGGCCCATTGGCGG‑GTTTGGAAGAACATACCGGTCAAGGGAATCCACCGCGACCCGATACAGCCCTTCCGCTTTTTCCGTTGATGCCGCCCGCGGTTTGTCCTTTTCCGCCCACAACTCCGCTATCCCGCCCTcc                                                       >  2:1162254/1‑150 (MQ=255)                                                                                                 ccAGAAATAGCGGGCCCATTGGCGG‑TTTTTGAAAAACATAACCGCCCCGGGAATCCAACCCGACCACACACAGCCCATTCACTTTTGTCCCTGCCGCCGCCAGCGCTCTTGCCTTTGCCAGCAGCAACCCTTCTATCCCCCCCTCCg                                                      >  1:635007/1‑147 (MQ=255)                                                                                                               cccATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGa                                    <  1:358332/151‑1 (MQ=255)                                                                                                                            tttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAgg                         <  1:1033985/150‑1 (MQ=255)                                                                                                                            tttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAgg                         <  1:845376/150‑1 (MQ=255)                                                                                                                            tttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGc                        >  2:663012/1‑151 (MQ=255)                                                                                                                             ttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGc                        <  1:880193/150‑1 (MQ=255)                                                                                                                              tGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCt                       <  1:932835/150‑1 (MQ=255)                                                                                                                              tGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTg                      >  1:1058601/1‑151 (MQ=255)                                                                                                                               ggAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGc                     <  2:1130817/151‑1 (MQ=255)                                                                                                                                  aGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGa                  <  1:1073729/151‑1 (MQ=255)                                                                                                                                               cAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGa      <  1:903495/150‑1 (MQ=255)                                                                                                                                               cAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAc     >  1:1173728/1‑151 (MQ=255)                                                                                                                                                  ggTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCat  >  2:479730/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGG‑TTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCAT  >  NC_000913/2434736‑2435030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑