Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,865,870	0	C	T	42.1%	37.8 / 17.8	38	S257F (TCC→TTC)	ygbN	putative transporter
Reads supporting (aligned to +/- strand):  ref base C (6/16);  new base T (16/0);  total (22/16)
Fisher's exact test for biased strand distribution p-value = 4.62e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTAGAAGTACTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGTACGGTTTCCGCAACACTGA‑TGCCGCCTTCGCATCCCCTGCTTGGTACGCTACAGCTGATCGGCTCACCAATGGTAGCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTAT  >  NC_000913/2865723‑2866020                                                                                                                                                    |                                                                                                                                                        cAGTAGAAGTACTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGTACGGTTTCCg                                                                                                                                                       <  1:519575/150‑1 (MQ=255) cAGTAGAAGTACTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGGACGGGTTTCg                                                                                                                                                       >  2:601604/1‑150 (MQ=255)    tAGAAGTACTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGGACGGTTTCTGCac                                                                                                                                                    >  1:507247/1‑149 (MQ=255)              ggAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGGTCGGGTTTCTCACCACTGA‑Agcc                                                                                                                                         >  2:297522/1‑150 (MQ=255)                  cAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGGACGGGTTCCCCCACCCCGC‑Tgccgcc                                                                                                                                      >  2:736452/1‑149 (MQ=255)                        cAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGGGCGGGTTTCGCCACACATT‑TTCCGCCTTCGcccc                                                                                                                              >  1:518382/1‑148 (MQ=255)                                 ccGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGTATGGGTTTCGCAACACTGA‑TGCCCCCCTCGCCTCCCCttcct                                                                                                                      >  1:353547/1‑146 (MQ=255)                                 ccGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGTACGGTTTCCGCAACACTGA‑TGCCGCCTTCGCATCCCCTGCt                                                                                                                       <  2:736377/149‑1 (MQ=255)                                 ccGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGGACGGGTTTCGCCACACTGC‑TGCCGCCCtcccctcccc                                                                                                                           >  1:366737/1‑145 (MQ=255)                                 ccGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGGACGGGTTTCGCCACACTGA‑TGCCGCCCTCGCCTcccc                                                                                                                           >  2:311717/1‑145 (MQ=255)                                             gAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGAAAGGGTTTCGCCACACCGA‑TGGCGCCCTCGCCCCCCCCCCCTTGTACGCTAc                                                                                                            >  2:123669/1‑148 (MQ=255)                                              aaGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGGGCGGTGTCCGCAACACTCA‑AGCCGCCTTCGCATACCCCCCTTGGTACGccgca                                                                                                           >  1:134605/1‑144 (MQ=255)                                                 gcgcGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGTACGGTTTCCGCAACACTGA‑TGCCGCCTTCGCATCCCCTGCTTGGTACGCTACAGCTGAt                                                                                                     <  1:435180/151‑1 (MQ=255)                                                 gcgcGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGGACGGGGTTCCCCACACCGA‑TGCCGCCCTTGCCCCCCCCGCTTGGTACGCcacaaccggt                                                                                                     >  1:61216/1‑145 (MQ=255)                                                              aaGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGTACGGTTTCCGCAACACTGA‑TGCCGCCTTCGCATCCCCTGCTTGGTACGCTACAGCTGATCGGCTCACCAAt                                                                                         <  2:313714/150‑1 (MQ=255)                                                              aaGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGTACGGTTTCCGCAACACTGA‑TGCCGCCTTCGCATCCCCTGCTTGGTACGCTACAGCTGATCGGCTCACCAATg                                                                                        <  2:366749/151‑1 (MQ=255)                                                                gCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGTACGGTTTCCGCAACACTGA‑TGCCGCCTTCGCATCCCCTGCTTGGTACGCTACAGCTGATCGGCTCACCAATGGt                                                                                      <  1:696264/151‑1 (MQ=255)                                                                gCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGTACGGTTTCCGCAACACTGA‑TGCCGCCTTCGCATCCCCTGCTTGGTACGCTACAGCTGATCGGCTCACCAATGGt                                                                                      <  1:324533/151‑1 (MQ=255)                                                                gCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGTACGGGTTTCGCCCCACTTA‑TTCCCCCCTCCCCTCCCCCGCTTTGTGCGCTACAGACGATCGGCTcccc                                                                                            >  2:28271/1‑145 (MQ=255)                                                                     aaaataaaTCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGTACCGTGTCCGCAAAACCTA‑AGCCGCCCTCACCTCCCCCCCTTTGTGAGCTATCGCTGATCCGCTCGcccctggggg                                                                                    >  1:666402/1‑140 (MQ=255)                                                                       aataaatCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGTACGGTTTCCGCAACACTGA‑TGCCGCCTTCGCATCCCCTGCTTGGTACGCTACAGCTGATCGGCTCACCAATGGTAGCTCTa                                                                               <  1:178833/151‑1 (MQ=255)                                                                        ataaatCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGTACGGTTTCCGCAACACTGA‑TGCCGCCTTCGCATCCCCTGCTTGGTACGCTACAGCTGATCGGCTCACCAATGGTAGCTCTaa                                                                              <  2:996500/151‑1 (MQ=255)                                                                           aatCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGTACGGTTTCCGCAACACTGA‑TGCCGCCTTCGCATCCCCTGCTTGGTACGCTACAGCTGATCGGCTCACCAATGGTAGCTCTAATGa                                                                           <  2:158187/151‑1 (MQ=255)                                                                               cACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGTACGGTTTCCGCAACACTGA‑TGCCGCCTTCGCATCCCCTGCTTGGTACGCTACAGCTGATCGGCTCACCAATGGTAGCTCTAATGATTgc                                                                       <  1:819831/151‑1 (MQ=255)                                                                               cACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGGACGGTTTTCGCAACACTGA‑TGCCGCCTTCCCATCCCCCCCCTTGTACGCTACCGCTGATCGGCTCCCCCATGGTAGCTCTTattatt                                                                         >  2:164909/1‑149 (MQ=255)                                                                                aCCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGGTCGGGTTTCGCAAACCCGA‑AGGCGCCCTCCCCTCCCCCGCCTGGTTCGCTACAGCCGGTCGGCCCACCAAAGGGAGATCTaa                                                                              >  2:574385/1‑143 (MQ=255)                                                                                   gggCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGTACGGTTTCCGCAACACTGA‑TGCCGCCTTCGCATCCCCTGCTTGGTACGCTACAGCTGATCGGCTCACCAATGGTAGCTCTAATGATTGCGCTg                                                                   <  2:704491/151‑1 (MQ=255)                                                                                          gcgcTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGGACGGGTTTCGCAACACTTA‑TTCCCCCCTCGCCTCCCCCCCTTGGTGCGCTACCGCCGATCGGCCCCCCCATGGTTGctctctttattttgctgg                                                                  >  1:484451/1‑131 (MQ=255)                                                                                          gcgcTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGGACGGGGTTCGCCACACCTA‑TACCCCCCTCCCATCCCCCGCCTGTTACCATACACCTTATCTGCCCCCCCAAGCTCGCACTACTTACTGTGCtggtggtgg                                                            >  1:933243/1‑151 (MQ=255)                                                                                              tGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGGAAGGGTGTCGCCACACTGA‑AAGCGCCCTCCCAAACCCCCCCTGGTTCGCCTCAACCGATCGGCTCCCCCATGGGAGCTCTAATTAATTggctggggctggggtt                                                        >  2:134047/1‑147 (MQ=255)                                                                                                           aaTTGTTATTCCTATCGCGATTATCATGGCGGGTGCGGGTTTCCCCACACTTATTTCCCCCCTC‑CCTCCCCCCCTTTGTGAGCTTCAACCGATCTGCGCCCCAACGGGCGCTCTCATGGTTTCGCCGGGGGTGGGGTTCTGGTtttt                                               >  1:1012633/1‑147 (MQ=255)                                                                                                                 tATTCCTATCGCGATTATCATGGCGGGGACGGGGTTCTCCCCACTGA‑TATCGCCCTCCCATCCCCCGCTTTGTACGCCACAGCTGATCGGCGCACCCAAGGGAGCCCTAATTATTTCGCTTGTGGTGGGCTTCTGGTTATTTTCtttttct                                     >  2:945181/1‑147 (MQ=255)                                                                                                                          cgcgATTATCATGGCGGGTACGGTTTCCGCAACACTGA‑TGCCGCCTTCGCATCCCCTGCTTGGTACGCTACAGCTGATCGGCTCACCAATGGTAGCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTgg                            <  1:598673/151‑1 (MQ=255)                                                                                                                              aTTATCATGGCGGGTACGGTTTCCGCGACACTGA‑TGCCGCCTTCGCATCCCCTGCTTGGTACGCTACAGCTGATCGGCTCACCAATGGTAGCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCt                        <  1:14235/151‑1 (MQ=255)                                                                                                                                   cATGGCGGGTACGGTTTCCGCAACACTGA‑TGCCGCCTTCGCATCCCCTGCTTGGTACGCTACAGCTGATCGGCTCACCAATGGTAGCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTacaa                   <  1:836194/151‑1 (MQ=255)                                                                                                                                        cGGGTACGGTTTCCGCAACACTGA‑TGCCGCCTTCGCATCCCCTGCTTGGTACGCTACAGCTGATCGGCTCACCAATGGTAGCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAacacac              >  1:280768/1‑151 (MQ=255)                                                                                                                                           gTACGGTTTCCGCAACACTGA‑TGCCGCCTTCGCATCCCCTGCTTGGTACGCTACAGCTGATCGGCTCACCAATGGTAGCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAg           <  2:207293/151‑1 (MQ=255)                                                                                                                                                 tttCCGCAACACTGA‑TGCCGCCTTCGCATCCCCTGCTTGGTACGCTACAGCTGATCGGCTCACCAATGGTAGCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACAt     >  2:632148/1‑151 (MQ=255)                                                                                                                                                    ccGCAACACTGA‑TGCCGCCTTCGCATCCCCTGCTTGGTACGCTACAGCTGATCGGCTCACCAATGGTAGCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTAt  >  2:730683/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                        CAGTAGAAGTACTGGAACAGATGCAACTGGCTCCGGCCAGTGAGGAAGGCGCGACAAAATTAAGCGATAAAATAAATCCACCGGGCGTCGCGCTGGTCACCTCGCTAATTGTTATTCCTATCGCGATTATCATGGCGGGTACGGTTTCCGCAACACTGA‑TGCCGCCTTCGCATCCCCTGCTTGGTACGCTACAGCTGATCGGCTCACCAATGGTAGCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTAT  >  NC_000913/2865723‑2866020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑