Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	29,496	0	T	A	48.6%	81.2 / 45.9	73	intergenic (+301/‑155)	dapB/carA	dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (30/7);  new base A (0/35);  total (30/43)
Fisher's exact test for biased strand distribution p-value = 6.46e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTG  >  NC_000913/29352‑29641                                                                                                                                                   |                                                                                                                                                  gTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATaaaaaa                                                                                                                                             <  2:327540/151‑1 (MQ=255)   aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAAt                                                                                                                                            <  2:99570/150‑1 (MQ=255)   aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAAt                                                                                                                                            <  1:1051770/150‑1 (MQ=255)   aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATc                                                                                                                                           >  1:455313/1‑151 (MQ=255)    aaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATcc                                                                                                                                          <  1:500824/151‑1 (MQ=255)     aTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATccc                                                                                                                                         >  1:1161693/1‑151 (MQ=255)          gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCa                                                                                                                                     <  1:600956/150‑1 (MQ=255)          gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCAt                                                                                                                                    >  2:386029/1‑151 (MQ=255)           aCCTTTTGGTCCTTTTTTTTCTGCTCGTTTTTATTTCATGAATTCTTCTTGCTGCGCAAGCGTTTTCA‑AAAAAAGGTTAGATAATTTTTTTGTCTCTAAATGCCTGAAAAACATGCG‑TGAGCCACAAAATAATATAAAAAACCCCGCCAt                                                                                                                                    <  1:255574/150‑1 (MQ=255)             cATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                                  >  2:958210/1‑150 (MQ=255)             cATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                                  >  2:285739/1‑150 (MQ=255)              aTTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                                  >  1:317589/1‑149 (MQ=255)                 tgttCCATTTTTTTCTTCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTCCC‑AAAACAGGTTAGATAATTTTTTTGTCGCTTAATCCCTGTAAAACATGCA‑GGGGCCAAAAAATAATATAAAAAACCCCGCCATTaa                                                                                                                                 <  1:751755/144‑1 (MQ=255)                 tGGTCAACTTTTTTATGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTg                                                                                                                             >  1:926141/1‑151 (MQ=255)                        cTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                                       >  2:163559/1‑150 (MQ=255)                         ttttttCTTCTCTTTTTTATTTCATGTAATCTTCTTGCTGCGCAGGCTTTTCCC‑AAAACATTTAAAATTATCTTTTTTTCGTTTAATGCTTATAAAACATGCA‑TGACCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGa                                                                                                                            <  2:193037/144‑1 (MQ=255)                         ttttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                                       >  1:691875/1‑149 (MQ=255)                           ttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAg                                                                                                                     >  1:296432/1‑149 (MQ=255)                                   tttttttttttTCATGCATTCTTCTTGCGGCAAAAGCTTTTCCA‑AAAACAGTTAAGATATTTTTTTTGTCTTTTAACGCTTAAAAAACAGGCA‑TGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCa                                                                                                               <  1:639434/144‑1 (MQ=255)                                     ttttttATTTGATCCCCTCTTCTTCCGGCGCACGCTTTTTCA‑AGAGCTGATAAGATAATTTTTTTGACTGTCAACCCATGTAAAAC‑TGTAGTGAGCCACAAAATACAAGAAAAAACTCCGCCATTAAGTTGACTTTTAGCGCCCatat                                                                                                            <  2:378769/147‑1 (MQ=255)                                       ttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                        >  1:56055/1‑150 (MQ=255)                                         ttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                        >  1:787566/1‑148 (MQ=255)                                            tttCATGAATTTTTCTTGCTGCGAAAGCTTTTTCA‑AAAACAGGTTAAATGTTTTTTTTGTCGTTAAATCCTTGAAAAACATGCA‑GGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                                   <  1:1024265/150‑1 (MQ=255)                                                   cAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGt                                                                                           >  1:119054/1‑151 (MQ=255)                                                   cAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGt                                                                                           >  2:183782/1‑151 (MQ=255)                                                         tcttGCGGCGCAAGCTTTTCCC‑AAAACGGGTTAGATGATTTTTTTGTCGTTTAATGCCTAAAAAACATGAA‑TGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGc                                                                                       <  2:929894/149‑1 (MQ=255)                                                         tcttGCGGCGCAAGCTTTTCCA‑AAAACAGGTTAGATGTTTTTTTTGTCGTTTAATCCCTGAAAAACATGCA‑TGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                         <  2:294597/147‑1 (MQ=255)                                                            tgctgcGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                                   >  2:956239/1‑150 (MQ=255)                                                                    aaGCTTTTTCC‑AGAACAGTTTAGATGATTTTTTTGTCGCTTATTGCCTGAAAAAAATGCA‑TGAGCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                            <  1:133604/149‑1 (MQ=255)                                                                     atgcttttCCAAGAAAAGTTTAAATTATTTTTTTGTCGTTAAATGCCTTAAAACCATGAA‑GGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                            <  1:210903/145‑1 (MQ=255)                                                                          tttCCAAGAACAGTTTAGATGTTTTTTTTGTCGTTTAATGCCTGAAAAACATGCA‑TGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCgg                                                                         <  2:18713/147‑1 (MQ=255)                                                                          tttCC‑AGACCAGGTAATATAATTTTTTGGCCCCTAATTGCTAGAAAAAAATCCT‑TGAGCCAAAAAAAAAAAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTaa                                                                      <  2:873924/149‑1 (MQ=255)                                                                              caaaaaCAGGTTAGATATTTTTTTTGTCGTTAATTCCCTGAAAAACATGCA‑TGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCa                                                                   <  1:937777/145‑1 (MQ=255)                                                                                aaaagagtttAGATGATTTTTTTTTCGCTAAAGTCCAAAAAACCAGGCA‑TGCCCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAt                                                                <  2:4223/142‑1 (MQ=255)                                                                                aGAAAAGGTGAGATGTTTTTTTTTCCGTTTAATGCATGAAAAACATGCA‑GGGCCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAtt                                                               <  2:657674/151‑1 (MQ=255)                                                                                  aacggtttAGTTGATTTTTTTGTCGCTAAATGCTTGAAAAACATGAA‑TGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTg                                                             <  2:967684/145‑1 (MQ=255)                                                                                        ttAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                        >  1:790851/1‑150 (MQ=255)                                                                                          aGATGTTTTTTTTGTCGTTAAATGCCTAAAAAACATGCA‑TGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                        <  2:232117/148‑1 (MQ=255)                                                                                           gatgatCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAttt                                                    >  1:44115/1‑151 (MQ=255)                                                                                             tgatCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTAc                                                  >  1:363822/1‑151 (MQ=255)                                                                                                ttttttttTCGTTTATTGCCTGTAAAACATGCA‑TGACCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                                 <  1:331695/147‑1 (MQ=255)                                                                                                tttttttGTGTCTTAATGCCTAAAAAACAGGCA‑AGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                                 <  1:305257/147‑1 (MQ=255)                                                                                                tttttttGTCTTTTAATGCCTGAAAAACAGGCA‑TGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                                 <  2:684083/147‑1 (MQ=255)                                                                                                tttttttGTCGTTAATTGCCTAAAAAACATGCA‑TGGCCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTAc                                                  <  1:508635/146‑1 (MQ=255)                                                                                                tttttttGTCGTTAAATCCCTGAAAAACATGAA‑TGAGCAAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                <  1:1035455/148‑1 (MQ=255)                                                                                                tCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                               >  1:608560/1‑151 (MQ=255)                                                                                                 cTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                               >  2:1043668/1‑150 (MQ=255)                                                                                                  ttttttTCGTTTAATGCTTAAAAACCAAGCA‑TTTCCAAAAAAATAATAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAt                                                      <  1:320724/142‑1 (MQ=255)                                                                                                  tttttGTCTTTTAATGCCTAAAAAACATGCA‑TGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtca                                              <  1:1116018/150‑1 (MQ=255)                                                                                                    tttGTCTTTTAATCCCTAAAAAAAAGGCA‑GGAGCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                            <  1:998252/150‑1 (MQ=255)                                                                                                     ttGTCTCTTAATCCCTTAAAAACATGCA‑TGACCAAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatca                                           <  1:1126109/150‑1 (MQ=255)                                                                                                     ttGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                          >  2:412587/1‑151 (MQ=255)                                                                                                     ttGTCGCTTAATCCTTGAAAAACATGCA‑GGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatca                                           <  2:909466/150‑1 (MQ=255)                                                                                                        tCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                                       >  1:322850/1‑151 (MQ=255)                                                                                                            ttAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                                   >  1:853697/1‑151 (MQ=255)                                                                                                            ttAATCCCTGTAAAACATGCA‑TGAGCCAAAAAATAATAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                                       <  2:1105151/147‑1 (MQ=255)                                                                                                                  cctaaaaaaCATGCA‑TGACCCAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                               <  1:436869/144‑1 (MQ=255)                                                                                                                      aaaaaCACCCA‑TGAGCACAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGATAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                            <  1:368061/147‑1 (MQ=255)                                                                                                                       aaaaaaTGCA‑TGAGCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaaa                        <  1:147962/151‑1 (MQ=255)                                                                                                                       aaaaCATGCA‑TGGGCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                            <  1:15190/147‑1 (MQ=255)                                                                                                                       aaaaCATGCA‑TGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa                          <  2:980624/149‑1 (MQ=255)                                                                                                                       aaaaCATGCA‑TGAACCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaaa                        <  1:732313/151‑1 (MQ=255)                                                                                                                           catgca‑tgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa                     >  2:473533/1‑150 (MQ=255)                                                                                                                              gca‑tgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                 >  2:838000/1‑151 (MQ=255)                                                                                                                                a‑tgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                 >  2:9944/1‑149 (MQ=255)                                                                                                                                 acggacaaaaaaaTAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaaa                    <  2:557719/137‑1 (MQ=255)                                                                                                                                    aGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg             >  2:408880/1‑150 (MQ=255)                                                                                                                                    aGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg             >  1:1108768/1‑150 (MQ=255)                                                                                                                                        aaaaaaaaataaaaaaatTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGaa           <  1:490622/146‑1 (MQ=255)                                                                                                                                        aaaaaaaaaaaaaaaaaaCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                 <  2:713841/140‑1 (MQ=255)                                                                                                                                         aaaaaTAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAtt       <  1:1002960/150‑1 (MQ=255)                                                                                                                                            aataatATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtctc    >  1:553853/1‑151 (MQ=255)                                                                                                                                            aataatATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtctc    >  2:156405/1‑151 (MQ=255)                                                                                                                                            aataatAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtctc    <  2:478426/151‑1 (MQ=255)                                                                                                                                             ataaaaAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTctct   <  1:407232/151‑1 (MQ=255)                                                                                                                                                ataaAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTg  <  2:236405/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTG  >  NC_000913/29352‑29641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑