Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,743,657	0	A	C	45.0%	72.6 / 32.3	60	T309P (ACC→CCC)	yiaK	2,3‑diketo‑L‑gulonate reductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (29/4);  new base C (0/27);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 3.62e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCG  >  NC_000913/3743508‑3743805                                                                                                                                                         |                                                                                                                                                      tttCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTtactac                                                                                                                                                      >  2:320369/1‑151 (MQ=255)    cacaAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTAccc                                                                                                                                                    <  1:773528/150‑1 (MQ=255)    cacaAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCc‑t                                                                                                                                                  <  2:828176/151‑1 (MQ=255)     acaAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCc‑tg                                                                                                                                                 >  1:22873/1‑151 (MQ=255)        aaTTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCc‑tgctg                                                                                                                                              >  1:650990/1‑151 (MQ=255)            tttATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGcc                                                                                                                                           <  1:1115544/150‑1 (MQ=255)              tATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGa                                                                                                                                         >  1:718468/1‑150 (MQ=255)               aTTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGaa                                                                                                                                        >  1:258684/1‑150 (MQ=255)                  gCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAAc                                                                                                                                     <  2:881445/150‑1 (MQ=255)                         aaGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTa                                                                                                                              >  2:590168/1‑150 (MQ=255)                         aaGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTa                                                                                                                              >  2:530858/1‑150 (MQ=255)                                 aaGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATc                                                                                                                      >  2:573825/1‑150 (MQ=255)                                    cTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACt                                                                                                                   >  1:11269/1‑150 (MQ=255)                                    cTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTg                                                                                                                  >  1:432767/1‑151 (MQ=255)                                       accgcggccccccccCGCGATGCCAAGCGGCACCGCACAATGGTTTCCGTTCCTAGGGCGGGGCGGGTTGAAAAAAACCGGGCAATTCGCTCCCCGGGCC‑A‑TAAT‑TTTACTCCCC‑TGGGGGCGAAAAACCGCCGTAACGGCATCACTGTtg                                                                                                               <  2:352267/142‑1 (MQ=255)                                        tCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTtg                                                                                                               >  1:672992/1‑150 (MQ=255)                                         cgccGGCCCCCCCCGCGATGCAACGTTGAAACGAATCATGGTTTACGTTCCGAGGCCCGAGGGTGCTGACAAAAACCAGCCCATCCCCTCCCCCGCCC‑A‑TGAT‑TTTTCTCCCC‑TCCGGGCAGAAATCCGCCGTAACGGCATCACTg                                                                                                                  <  1:1151351/143‑1 (MQ=255)                                           aCGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCTGAAAACCGCCGTAACGGCATCACTGTtgatg                                                                                                            >  1:571432/1‑150 (MQ=255)                                            cGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTtgatg                                                                                                            >  1:453490/1‑149 (MQ=255)                                             ggcccccccCGCGATGCAAAGCGGAAACCAATATGGGTTTCCGTTACTGGTGCGGGGGGGGCTGAAAAAAATCGGCCCATCCGCTTCCCCGCCA‑A‑TAAT‑TTTCCTCCCC‑TGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGAc                                                                                                          <  2:642289/147‑1 (MQ=255)                                               tCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGc                                                                                                       >  2:1014553/1‑151 (MQ=255)                                                  cccccGCGATGCCAAGCTGCACCGCATAAGGGTTTACGTTACTAGGGCCGGGCGTGCTGACAAAAACCAGCCCATCCGCTTCCCCGGCC‑A‑TAAT‑TTTACTCCCC‑TGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCgtg                                                                                                    <  2:702640/149‑1 (MQ=255)                                                    cccgcggtcaaaaGCTGCAACGAATCGTGTTTTACGTTACTGGGCCGGGGCGTGCTGACAAAAATAAGGCCTTCCTCTCCCCCGGCC‑A‑TAAT‑TTTACTCCCC‑TGCGGGCCAAAACCCGCCGTAACGGCATCACTGTTGATGACAGCgtgt                                                                                                   <  1:106549/139‑1 (MQ=255)                                                      cgcgATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTggg                                                                                                >  2:635300/1‑151 (MQ=255)                                                            gCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCaaa                                                                                           >  1:1015234/1‑150 (MQ=255)                                                               aaGCTGCAACCAATCAGGGTTTACTTTCCTGGTGCCGGGCGTGAGGAAAAAAACCAGCCCTTTCGCTT‑CCCGGCC‑A‑AAAATTTTACTCCCC‑TGCGGCCAAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTgg                                                                                                 <  1:734359/141‑1 (MQ=255)                                                                   taaaacacaaaatGGATTGCTTTACTAGCGCGGGGCGTGTTGACAAAAAACGGGCCCTTCG‑TTTCCCGGCCAA‑TAAT‑TTTCCCCCCC‑GGCGGCCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAg                                                                                     <  1:1004806/138‑1 (MQ=255)                                                                      accGCATCATGTTTTCCTTTACTGGTGCGGGGCGTGCTGAAAAAAATCAGCCCTTCCTCTTCCCCGGCC‑A‑TAAT‑TTTCCTCCCC‑TGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAgg                                                                                    <  2:669831/145‑1 (MQ=255)                                                                              atGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGCCAGCGTGTGGGCCAAAGTCCAGGCGTTATgagg                                                                          >  1:1008267/1‑149 (MQ=255)                                                                                gttttccGTTACTGGGGCGGGGGGTGCTGAAAAAAATCAGGCCTTTCGCTTCCCCGGCC‑A‑TAAT‑TTTCCTCCCC‑TGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATgag                                                                           <  2:861341/140‑1 (MQ=255)                                                                                ggATTACTTTACTAGGGCCGGGCGTGCTGAAAAAAACCGGCCCTTTCGTTTCCCCGCCA‑A‑TGAA‑TTTACTCCCC‑TGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATgag                                                                           <  2:1135027/146‑1 (MQ=255)                                                                                 gATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGAt                                                                      >  1:314861/1‑150 (MQ=255)                                                                                 gATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGAt                                                                      >  1:109984/1‑150 (MQ=255)                                                                                   ttcctttACTAGGGCCGAGCGTGCGGAAAAAAACCAGCCCATTCCCTTACCCGCCC‑A‑TAAA‑TTTACTCCCC‑TGCGGCCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGAt                                                                      <  2:807822/143‑1 (MQ=255)                                                                                   ttagtttACTGGGGCGGAGCGTGCTGACAAAAACCAGCCCATTCGTTTCCCCGGCC‑A‑TAAT‑TTTACTCCCC‑TGCTGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATgagga                                                                         <  2:1036799/140‑1 (MQ=255)                                                                                   ttACGTTACTGGGCCCGGGCTTGCGGAAGAAAAGCAGCCCATGCGCTCCCCCGCCC‑A‑TGAT‑TTTCCCCCCC‑GCCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATa                                                                     <  2:316978/149‑1 (MQ=255)                                                                                      cGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTc                                                                 >  1:82881/1‑150 (MQ=255)                                                                                        taagtggtcccGGGCGTGCGGAAAAAATCCAGCCCTTCCGCTCCCCCGGCC‑A‑TAAA‑TTTCCCCCCC‑GGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGt                                                                  <  2:881950/138‑1 (MQ=255)                                                                                          aCTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGAt                                                            >  1:1126774/1‑151 (MQ=255)                                                                                            tAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGAttt                                                          >  1:269044/1‑151 (MQ=255)                                                                                                gCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTgg                                                      >  1:798192/1‑151 (MQ=255)                                                                                                ccggAGCGTGTAGAAAAAAACCAGGCCATCCGTTCCCCCGGCC‑A‑TGAA‑TTTACTCCCC‑TGTTGCCAAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTg                                                       <  2:258943/147‑1 (MQ=255)                                                                                                 ccGGGCGTGCTGAAAAAAACCAGGCCATCCGCTCCCCCGGCC‑A‑TAAT‑TTTCCTCCCC‑TGCGGGCGAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGa                                                     <  2:388286/151‑1 (MQ=255)                                                                                                   gggCGTGCTGACAAAAACCAGCCCTTTCGCTTCCCCGGCA‑A‑TAAT‑TTTACTCCCC‑GGCTGGCAAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGAc                                                    <  2:1127464/148‑1 (MQ=255)                                                                                                       gTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATc                                               >  1:325557/1‑151 (MQ=255)                                                                                                         gctaaaaaaaaTCAGCCATTTCTCTTCCCCGGCC‑G‑TAAT‑TTTCCTCCCC‑GGTTGGCAAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGAc                                                    <  2:1114833/137‑1 (MQ=255)                                                                                                                aaaaCCAGGCCTTTCGCTTCCCCGGCC‑A‑TGAT‑TTTACTCCCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGcc                                       <  2:808322/150‑1 (MQ=255)                                                                                                                 aaaTCACGCCATCCGCTTCCCCGACT‑A‑TGAT‑TTAACTCCCC‑TGGGGGCAAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGccc                                      <  1:825071/150‑1 (MQ=255)                                                                                                                         ccATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGc                             >  1:1083686/1‑151 (MQ=255)                                                                                                                            tccccTTCCCCGCCC‑A‑TGAT‑TTTCCTCCCC‑TGCGGCCAAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATcc                                 <  2:1126833/140‑1 (MQ=255)                                                                                                                              cGCTCACCCGGCC‑A‑TAAA‑TTTCCTCCCC‑TGCTGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCg                           <  1:537232/148‑1 (MQ=255)                                                                                                                               ccctccccGGCC‑ATTAAA‑TTTCCTCCCC‑GGGGGGCCAAAACCCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATcc                                 <  1:739863/137‑1 (MQ=255)                                                                                                                                 ttccccGGCA‑A‑TAAT‑TTTCCTCCCC‑TGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGcc                            <  2:982025/141‑1 (MQ=255)                                                                                                                                    cccGCCC‑A‑TGAA‑TTTCCCCCCC‑TGCTGCCGGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGc                      <  2:60625/147‑1 (MQ=255)                                                                                                                                        gCC‑A‑TGTA‑TTTACCCCCCTTGCGGGCAAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCccgcc                 <  1:627940/149‑1 (MQ=255)                                                                                                                                            a‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGGGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCAtt           >  2:1052794/1‑151 (MQ=255)                                                                                                                                               gAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTg          >  1:257221/1‑150 (MQ=255)                                                                                                                                                 attttCCTCCCC‑TGCGGGCCAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCgccgcc              <  2:1079605/143‑1 (MQ=255)                                                                                                                                                 a‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCACAATCCAGGCGTTCTGAGGAGATCAGTCATGATTTTTGGACATATCGCGCAGACCAATCCGTGCCGTTTGCCCGCCGCCATTg          >  1:189015/1‑148 (MQ=255)                                                                                                                                                 a‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGaa        >  2:145285/1‑150 (MQ=255)                                                                                                                                                    ttACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGaaa       >  2:251430/1‑149 (MQ=255)                                                                                                                                                       ctacCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGgcg  >  1:1148024/1‑151 (MQ=255)                                                                                                                                                         |                                                                                                                                                      TTTCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAAGCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑A‑TGAA‑TTTACTACCC‑TGCTGGCCGAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAAAGGCG  >  NC_000913/3743508‑3743805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑