Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,423,294	0	T	C	41.8%	111.4 / 28.4	79	intergenic (+100/+129)	yhdZ/rrfF	putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
Reads supporting (aligned to +/- strand):  ref base T (11/35);  new base C (33/0);  total (44/35)
Fisher's exact test for biased strand distribution p-value = 6.42e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGT‑CTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTAC  >  NC_000913/3423149‑3423441                                                                                                                                                   |                                                                                                                                                         tAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GGCCTTCcccccccccc‑cc                                                                                                                                                       >  2:187864/1‑148 (MQ=255)  aaaTCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCgg                                                                                                                                                     <  1:670169/149‑1 (MQ=255)     tCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCcccccccccc‑cc                                                                                                                                                       >  1:85465/1‑144 (MQ=255)     tCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GGCCTGCcccccccccc‑cc                                                                                                                                                       >  1:80428/1‑144 (MQ=255)          gCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTTCCCCCCCCCCC‑CCCGGCCGATGcgt                                                                                                                                           >  2:618356/1‑149 (MQ=37)          gCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑t                                                                                                                                           <  1:903058/150‑1 (MQ=255)           cGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTTGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCCTCCCCCCCCCCC‑CCcgg                                                                                                                                                    >  2:401393/1‑141 (MQ=37)           cGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCCCCCC‑CCCGGCCGGTGC‑tt                                                                                                                                          >  1:722549/1‑150 (MQ=255)                    gCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCCTCCCCCCACCCC‑CCCGGCCGCTGC‑TTTTCAACgct                                                                                                                                 >  1:1474214/1‑148 (MQ=25)                     cATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCCCCCCCCC‑CCCCGCCCTTGC‑TTCCCAACGtt                                                                                                                                 >  1:712209/1‑149 (MQ=25)                      aTTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCCCCCC‑CCCGGCCGTTGC‑TTCGCAACGtt                                                                                                                                 >  2:1335223/1‑148 (MQ=255)                            aTCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GGCCTCCCCCCCCCCCC‑CCCGGCCGGTGC‑TTCCCCACGTTCTAATCCGc                                                                                                                        >  2:472788/1‑151 (MQ=255)                              cGCAGGTAATCCATTAATTGAACGTTAGTTCGAAAAGCAAAAAGGCAATCCTTGCGGATGGCCTTCCGCTTGATTTGATGTGTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCAGCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCt                                                                                                                       <  1:534012/150‑1 (MQ=18)                                 aGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGG‑GTCCTGCCCCCCACCCC‑CCGGGCCGCTGC‑TTCGCAACGCTCAAATCCACTccc                                                                                                                    >  1:1181209/1‑150 (MQ=255)                                 aGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTTCCCGCCCCCCC‑CCCGGCCGCTGC‑TTCGCAACGTTCAAATcccccccc                                                                                                                    >  2:223261/1‑150 (MQ=21)                                 aGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCCCCACCCC‑CCGGGCCGGTGC‑TTCGCAACGTTCAAATCCGCcccc                                                                                                                    >  2:89925/1‑150 (MQ=255)                                  ggTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCcgg                                                                                                                  <  2:14852/151‑1 (MQ=255)                                      aTCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGa                                                                                                              <  1:347259/151‑1 (MQ=255)                                        ccATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGAt                                                                                                             <  2:152570/150‑1 (MQ=255)                                          aTTAATTGAATGTTCGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGGCCCGCCC‑CCCCCCC‑CCCCGCCGTTTC‑TTCGCAACCGTCCAATCCCCACCCGGCGGGttt                                                                                                           >  1:1181423/1‑150 (MQ=255)                                              aTTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGG‑GGCCTCCCCCCCCCCCC‑CCCGGCCGTTGCTTTTCCAACCTT‑AAAACCGCTCCccgcggggtttt                                                                                                         >  1:193098/1‑142 (MQ=255)                                               ttGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGG‑GGCCTCCCCCCCCCCCC‑CCCGGCCGGTGC‑TTTGCAAAGGTTAAAAACGCCCCCCGCGGAgtttt                                                                                                         >  1:946466/1‑145 (MQ=255)                                                 gAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACt                                                                                                   <  2:1444824/151‑1 (MQ=255)                                                 gAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCCGTCCGCCCCCCT‑CCCCGCCGTTGC‑TTTCCAACCTTCAAATACGCTCCCCGCGGGTTTGTTCTAc                                                                                                    >  1:1037415/1‑150 (MQ=18)                                                 gAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCCGCCCCCCACCCC‑CCCGGCCCGTTC‑TTCGCAACATTCCAATTCGCCCCCCGCGGGttt                                                                                                           >  2:766654/1‑143 (MQ=18)                                                  aaTGTTAGTTCGAAAATCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACt                                                                                                   <  1:1316659/150‑1 (MQ=255)                                                  aaTGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑CTCCCCCCCCCCACCCTCCCGGGCGGTTGC‑TT‑GCAACGGTCAAAATCGCCCCccgcgggcgtgttcttc                                                                                                    >  1:1558267/1‑138 (MQ=255)                                                    tGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GGCCTGCCCCCCACCCC‑CCCGGCCGTTTC‑TTTGCCACCGTCCAATTCCCCCCCCGCGGGGTTTTTCTACT‑Cgg                                                                                               >  2:273034/1‑151 (MQ=255)                                                           tCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCCCTTGATTTGATGTCTGGCAGTTTAGGGCGGGC‑GTCCTGCCCGCCGCCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGgaga                                                                                           <  1:224941/148‑1 (MQ=255)                                                            cGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTTCTGCCCGCCACCCC‑CCGGGCCGTTGC‑TTTGCAAAGGTTAAAACCCCCCCCCGCGGGTTTTTCCTACT‑CCGGagaggg                                                                                        >  2:1234928/1‑148 (MQ=17)                                                            cGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTTCCCGCCACCCC‑CCCGGCCGTTGC‑TTTGCAAACTTCAAATCCCCCCCCCGCGGGTTTGTCCTTCT‑CCGGggag                                                                                          >  1:46522/1‑148 (MQ=17)                                                            cGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAgtg                                                                                        <  1:1380567/150‑1 (MQ=255)                                                             gAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GGTCTGCCCGCCCCCCT‑CCGGGCCGTTGC‑TTTGCAACGTTTAAATCCCCCCCCCGCCGGTTTTTTCTTCT‑Ccgggggggggt                                                                                      >  2:149389/1‑143 (MQ=255)                                                              aaaaGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCCGCCCCCCCCCCC‑CCCGGCCGTTGC‑TTCGCCACGGTCCAATTCCCCCCCCGCGGGTTTTTCCtcccccgggggggg                                                                                         >  1:516865/1‑135 (MQ=17)                                                                aaGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GGCCCTCCCCCCCCCCC‑CCCGGCCGGTGC‑TTCTCAACCTTCCAAAACCCCCCCCCCGGCTTTTTCCTACT‑CCGGGGAGTGGTccc                                                                                   >  1:40325/1‑149 (MQ=255)                                                                 aGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCAcc                                                                                  <  1:733631/151‑1 (MQ=255)                                                                 aGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCCCCCCCCT‑CCCGGCCGTTGC‑TTCGCAACGTTCAAAACCCCTCCCCGCGGGTTTTTTCTCCT‑CGGGGGAGTGTTCAcc                                                                                  >  2:1531773/1‑151 (MQ=17)                                                                 aGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCCCCCCCCC‑CCCGGCCGGTTC‑TTCGCAACCTTTCAAACCCCCCCCCGCGGGTTTTTCCTACT‑CCGGGGAGTGTTCAcc                                                                                  >  2:1281033/1‑151 (MQ=17)                                                                  gCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCg                                                                                 <  2:1350407/151‑1 (MQ=255)                                                                   cAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GGCCCTCCCCCCCCCCC‑CCCGGCCGTTGC‑TTCGCAACATTCAAAACCGCCCCCCGCGGGTTTTTTCTACT‑CCGGGGAGGGTTCAcc                                                                                  >  2:799654/1‑149 (MQ=14)                                                                     aaaaGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTTCCCCCCACCCC‑CCGGGGCGTTGC‑TTCGCAAAAGTCAAATCCGCCCCCGGCGGGTTTGTCCTACT‑CCGGGGGGGGTTTACCCac                                                                               >  1:759341/1‑150 (MQ=14)                                                                     aaaaGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGaca                                                                              <  1:1088547/151‑1 (MQ=255)                                                                        aGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGacaaac                                                                           <  1:565236/151‑1 (MQ=255)                                                                        aGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGacaaac                                                                           <  2:1512922/151‑1 (MQ=255)                                                                         ggCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGacaaaca                                                                          <  1:509101/151‑1 (MQ=255)                                                                         ggCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTGATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGacaaac                                                                           <  1:570317/150‑1 (MQ=255)                                                                           ccATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAaacaac                                                                        <  1:403092/151‑1 (MQ=255)                                                                            cATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCCGCCCCCCCCCCC‑CCCGGCCGCTGC‑TTCTCAACGCTCCAATCCGCCCCCCGCGGGTTTGTTCTTCT‑CCGGAGAGTGGTTCCCCACCaacaac                                                                        >  1:1412998/1‑150 (MQ=12)                                                                                   tCGGATGGCCTGTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATaaa                                                                <  2:93245/150‑1 (MQ=255)                                                                                    cGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAATGTTCACCGACAAACAACAG‑ATAAAAc                                                              <  2:572250/151‑1 (MQ=255)                                                                                     ggATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GGCCCGCCCCCCACCCT‑CCGCGGCGGTGC‑TTCTCAAACGTCCAATACGCTCCCCGCGGGTTTTTTCTTCT‑CGGGAGGGGGTTTTCCCGCAAAACAAAG‑ATaaaa                                                               >  1:1326605/1‑149 (MQ=255)                                                                                       aTGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCCGCCCGCCCCCCC‑CCCGGGCGGTTC‑TTTGCCAAGTTTCAATACGCTCCCCGCCGCCTTGTTCTTCT‑CGGGGGCGGGTTCCCCCCCAAAAAACCG‑GTaaaa                                                               >  1:295566/1‑147 (MQ=9)                                                                                        tGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCCCCCC‑CCCGGCCGTTTC‑TTTTCAAAGTTCAAATCCCCCCCCCGCGGGTTTGTCCTTCT‑CCGGGGAGGGGTTACCCACAAAAAACAG‑ATAAAACaaa                                                           >  2:490196/1‑150 (MQ=14)                                                                                         ggCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GGCCTGCCCGCCACCCC‑CCCGGCCGTTGC‑TTTGCAACCTTCAAAACCGCCCCCCGCGGATTTTTCCTACT‑CCGGAGAGTGTTTACCCACAAACAACAG‑ATAaaaaaaaa                                                          >  2:286705/1‑150 (MQ=255)                                                                                          gCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑TTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAgg                                                        <  2:496127/151‑1 (MQ=255)                                                                                             tttCGCTTGATTTGATGTCTGGCAGTTTATGGCGGTC‑GCCCTCCCCGCCCCCCC‑CCGGGCCGGTGT‑TTCCCCACCTTTCAATTCGCTCCCCGGGGGTTTTTCCCACT‑CGCGGGGGGGTTTCCCGCCAAACAACCA‑ATTTAACAAAAAGccc                                                     >  2:950598/1‑151 (MQ=255)                                                                                                cGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCCCCCC‑CCCGGCCGGTGC‑TTCGCAACGTTTAAATCCGCTCCCCGCGGGTTTTTCCTACT‑CCGGGGAGGGTTCACCCACCAACAACAG‑ATAAAAAAAAAAGccc                                                     >  2:1509221/1‑148 (MQ=11)                                                                                                cGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGt                                                  <  2:521988/151‑1 (MQ=40)                                                                                                    tgatttgatGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGT‑Ct                                               <  1:1350418/149‑1 (MQ=40)                                                                                                    tgatttgatGTCTGGCAGTTTATGGCGGGC‑GTCCTCCCCCCCCCCCC‑CCCGGCCGTTTC‑TTCGCAACGTTTAAAACCCCCCCCCGCGGGTTTTTCCTACT‑CGGGAGAGGGTTCACCGACCAAAAAAAG‑ATAAAAAAAAAAGCCCAGT‑Ctt                                              >  2:508746/1‑150 (MQ=11)                                                                                                        ttgatGTCTGGCAGTTTATGGCGGGC‑GTCCCGCCCGCCCCCCT‑CCGGGCCGGTTC‑TTCGCAACGTTCCAATCCCCCCCCCGCGGGGTTGTTCTACC‑CGGGAGAGGGTTCACCCACCAACAACCG‑GTAAAACAAAAAGCCCAGT‑Ctt                                              >  2:59680/1‑146 (MQ=11)                                                                                                         tgatGTCTGGCAGTTTATGGCGGGC‑GTCCCGCCCGCCCCCCT‑CCCGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGGTTTGTCCTACT‑CGGGAGAGGGGTTACCGACAAACAAAAG‑ATAAAAAAAAAAGGCCAGT‑CTTCCGAc                                         >  1:43387/1‑150 (MQ=11)                                                                                                          gatGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGT‑CTTCCGACTg                                       <  2:734655/151‑1 (MQ=38)                                                                                                           atGCCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCCCCCC‑CCCGGCCGGTGC‑TTTGCAACGGTCAAATACGCCCCCCGCCGGTTTTTTCTTCTCCGGGGGGGGGTTC‑CCCACAAAAAACAGAAAAAAAAAAAAGGcccggcttt                                               >  1:950907/1‑138 (MQ=0)                                                                                                             gTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGT‑CTTCCGACTGAg                                     <  2:198437/150‑1 (MQ=38)                                                                                                                   cAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGT‑CTTCCGACTGAGCCTTTTg                              <  1:149368/151‑1 (MQ=37)                                                                                                                    aGTTTATGGCGGGC‑GTCCTGCCCCCCACCCC‑CCCGGCCGGTTC‑TTTGCAAAGGTCAAAACCGCCCCCGGCGGGGTTTTTCTACT‑CGGGGGGGTGTTTACCGACCAACAACAG‑ATAAAACAAAAAGCCCAGTCCTTCCG‑CTGAGCCcttttt                             >  1:887966/1‑149 (MQ=2)                                                                                                                    aGTTTATGGCGGGC‑GTCCCGCCCGCCACCCC‑CCCGGCCGCTGC‑TTCGCAAAGTTCAAATCCGCCCCCGGCGGGTTTTTCCTACT‑CGGGAGAGTGTTCACCCACAAACAACAG‑ATAAAACAAAAAGCCCCGT‑CTTCCCACTGAGCCTTTTGt                             >  2:439901/1‑151 (MQ=255)                                                                                                                         aTGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGT‑CTTCCGACTGAGCCTTTTGTTTTAt                        <  1:1540967/151‑1 (MQ=38)                                                                                                                         aTGGCGGGC‑GTCCTGCCCGCCACCCC‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGGGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAAGCCCAGT‑CTTCCGACTGAGCCTTTTGtttttt                        >  1:820107/1‑149 (MQ=255)                                                                                                                           ggcgggc‑gTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGT‑CTTCCGACTGAGCCTTTTGTTTTAttt                      <  2:1166474/151‑1 (MQ=38)                                                                                                                             cgggc‑gTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGT‑CTTCCGACTGAGCCTTTTGTTTTATTTg                     >  2:883116/1‑150 (MQ=38)                                                                                                                              gggc‑gTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGT‑CTTCCGACTGAGCCTTTTGTTTTATTTGa                    <  2:1023319/150‑1 (MQ=38)                                                                                                                              gggc‑gTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGT‑CTTCCGACTGAGCCTTTTGTTTTATTTGAt                   <  2:1402213/151‑1 (MQ=38)                                                                                                                                 c‑gTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGT‑CTTCCGACTGAGCCTTTTGTTTTATTTGATGTc                >  1:916677/1‑151 (MQ=39)                                                                                                                                 c‑gTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGT‑CTTCCGACTGAGCCTTTTGTTTTATTTGATGTc                >  1:703506/1‑151 (MQ=39)                                                                                                                                   gTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGT‑CTTCCGACTGAGCCTTTTGTTTTATTTGATGTCt               >  1:1229164/1‑151 (MQ=40)                                                                                                                                    tCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGT‑CTTCCGACTGAGCCTTTTGTTTTATTTGATGTCt               <  2:712295/150‑1 (MQ=40)                                                                                                                                     ccTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGT‑CTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTgg             <  1:222293/151‑1 (MQ=40)                                                                                                                                     ccTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGT‑CTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTg              <  2:623691/150‑1 (MQ=40)                                                                                                                                             ccACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGT‑CTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTccc     <  1:1531624/151‑1 (MQ=40)                                                                                                                                                 ccT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGT‑CTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTa   <  1:1270981/149‑1 (MQ=40)                                                                                                                                                   t‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCCCCGACAAACAACAG‑ATAAAACAAAACGCCCAGT‑CTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTAc  >  1:1277972/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                                         TAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGC‑GTCCTGCCCGCCACCCT‑CCGGGCCGTTGC‑TTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGT‑CTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTAC  >  NC_000913/3423149‑3423441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑