Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,434,882	0	T	G	45.3%	109.8 / 29.5	75	Y252S (TAC→TCC)	truA	tRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base T (7/34);  new base G (34/0);  total (41/34)
Fisher's exact test for biased strand distribution p-value = 9.13e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATAT  >  NC_000913/2434736‑2435032                                                                                                                                                    |                                                                                                                                                          gTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATc                                                                                                                                                       <  1:1018911/151‑1 (MQ=255)  tCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGGTTTGGAAGATCATACCGGGCAGGGGaa                                                                                                                                                         >  2:428161/1‑148 (MQ=255)   ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCATAACCGGCAGGGGaaaac                                                                                                                                                      >  1:149344/1‑147 (MQ=255)   ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGGAGATCATACCGGGCAGGGTaaaac                                                                                                                                                      >  2:971191/1‑147 (MQ=255)   ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCa                                                                                                                                                     <  2:61420/151‑1 (MQ=255)     atatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGGAGAATATACCGGGCAGGGGAATCCAcc                                                                                                                                                   >  2:1280970/1‑151 (MQ=255)         ttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCATACCGGTCAGGGGAAACCACcgc                                                                                                                                                 >  1:168493/1‑149 (MQ=255)                     tGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑Ag                                                                                                                                    <  1:748671/149‑1 (MQ=255)                     tGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGc                                                                                                                                   <  1:184188/150‑1 (MQ=255)                       tACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCATACCGGGCAGGGGAAACCACCCCGACCAGATAC‑AGccc                                                                                                                                 >  1:217715/1‑150 (MQ=255)                        aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCATACCGGGCAGGGGAATACACCCCGACCAGAAAC‑AGcccct                                                                                                                               >  1:955573/1‑149 (MQ=255)                         cTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCATACCGGGCAGGGGAATCCACCGCGACCCGATAC‑AGccc                                                                                                                                 >  2:243673/1‑148 (MQ=255)                          tCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCGCGACCCGATAC‑AGCCCCTcc                                                                                                                             >  1:1265192/1‑151 (MQ=255)                           cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTc                                                                                                                              <  1:301529/149‑1 (MQ=255)                                  tttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGGTTTTGTAGATAATAACGGACAGGGGAATTCAACGCGACCAGAGAC‑AGGCCTTCCGG‑tttt                                                                                                                      >  2:743890/1‑149 (MQ=255)                                    tCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGGAATTCACCCCGACCAGATAC‑AACCCTTCCGC‑ctttttc                                                                                                                   >  1:956027/1‑147 (MQ=255)                                     cAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCCGGGGAATCCACCGCGACCAGATAC‑AACCCCTCCGC‑TTTTTcc                                                                                                                   >  2:437672/1‑149 (MQ=255)                                      aGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATTATACCCGGCAGGGGAATCCACCGCGACCAGATAA‑AACCCCTCCGC‑CTTTTCCGtt                                                                                                                >  2:1146523/1‑151 (MQ=255)                                      aGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGtt                                                                                                                <  2:1101695/151‑1 (MQ=255)                                      aGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGtt                                                                                                                <  2:940465/151‑1 (MQ=255)                                       ggCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTTGGAGGA‑CATACCCGGGCGGGGAATCCACCCCCACCCAAAACAAACCCTTCCCC‑ttttccgct                                                                                                                 >  1:801165/1‑145 (MQ=255)                                          tGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGTAGATCATATCAGGCAGGGGAAATCACCCCCACCAGAAAA‑AAACCTTCCGC‑TTTTTGCGTtgc                                                                                                              >  2:437871/1‑149 (MQ=255)                                           gTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCATACCGGGCCGGGGAATCCACCCCGACCAGAAAC‑AGCCCTTCCCC‑TTTTTcc                                                                                                                   >  1:728866/1‑143 (MQ=255)                                              aCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTgccgc                                                                                                        <  1:234744/151‑1 (MQ=255)                                                       gCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGtt                                                                                                 <  1:20336/149‑1 (MQ=255)                                                        cTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGAAGATCATAACCGTCAGGGGAATTCACCCCGACCAGATAC‑AGGCCCTCCCC‑TTTTTCCGTTGCTGCCCCCAGCGTTCt                                                                                               >  1:268512/1‑150 (MQ=255)                                                         tACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCATACCCGGCAGGGGAAATCACCCCGACCAGATAC‑AGCCCCTCCCC‑CTTTTCCGTTTCTGCCGCCCGCGGTCt                                                                                               >  1:1230946/1‑149 (MQ=255)                                                                tGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATAC‑AGCCCTTCCCC‑TTTTTCCGTTGCTGCCGCCCGCGTTCTTT‑CCttt                                                                                       >  2:406578/1‑149 (MQ=255)                                                                  aaaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTgccg                                                                                   <  1:154084/151‑1 (MQ=255)                                                                      gTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAg                                                                               <  2:24512/151‑1 (MQ=255)                                                                       ttCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCATACCGGGCAGGGGAATCCACCGCGACCACATAC‑AACCCCTCCCC‑TTTTTCCGTTGGTGCCGCCCGCGTTCTGT‑TCTTTgtcccc                                                                                 >  1:750534/1‑143 (MQ=255)                                                                         ccGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCa                                                                             <  1:276910/150‑1 (MQ=255)                                                                          cGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCCTACCGGTCAGGGGAATCCACCCCGACCCGATAC‑AGCCCCTCCCC‑TTTTTCCGTTGCTGCCGCCCGCGGTCTGT‑CCTTTTCCGCCCGCaa                                                                            >  2:1156922/1‑150 (MQ=255)                                                                          cGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCCCGACCCGATAC‑AACCCCTCCGC‑TTTTTCCCTTGCTGCCCCCCGCGGTCTGT‑CCTTTTccgcccgcca                                                                            >  1:1201662/1‑148 (MQ=255)                                                                           gAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAAct                                                                          <  2:780959/151‑1 (MQ=255)                                                                            aGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCATACCGGGCCGGGGAATCCACCCCGACCAAATAC‑AGCCCTTCCGCTTTTTGCCTTGGCTGCC‑CCAGCCGTCTGT‑CCTTTGCCCCCAg                                                                               >  2:732605/1‑145 (MQ=255)                                                                               attattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGGAGATCATACCGGGCAGGGGAATCCACCCCGACCAGATAC‑AACCCTTCCGC‑TTTTTCCGTTGCTGCCGCCAGCGGTCTGT‑CCTTTTCCGCCCGCAActc                                                                         >  2:1062845/1‑148 (MQ=255)                                                                                ttattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATTATAACCGGCAGGGGAAACCACCCCGACCCGATAC‑AGCCCCTCCCC‑CTTTTCCGTTGCTGCCCCCCGCGGTCTGT‑CCTTTTCCCCCCGCAAatc                                                                         >  1:611573/1‑147 (MQ=255)                                                                                   ttCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGAAGATCATACCGGTCCGGGGAATCCACCGCGGCCAGATAA‑AACCCCTCCCC‑TTTTTCCGTTGGTGCCCCCAGCGTTCTGT‑CCTTTTCCGCCAGCAACCCTGCTa                                                                    >  2:604899/1‑149 (MQ=255)                                                                                     ctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATAC‑AACCCTTCCCC‑TTTTGCCGTTGCTGCCCCCAGCGTTCTGT‑CCTTTTCCGCCAGCAACTCTGCTATcc                                                                 >  2:972918/1‑150 (MQ=255)                                                                                       cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCATAACGGTCAGGGGAATCCACCGCGACCAGAAAC‑AACCCCTCCGC‑TTTTTCCCGTTCTGCCCCCCGCGTTCTGT‑CCTTTTCCGCCCGCAACTCTGCTATCCAGc                                                              >  2:401017/1‑151 (MQ=255)                                                                                       cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCATAACGGGCAGGGGAATCCACCCCGACCAGATAC‑AACCCCTCCCC‑TTTTTCCGTTTCTGCCCCCAGCGGTCTTT‑CCTTTGCCGCCAGCAACTCTTCCATcc                                                                 >  2:967778/1‑148 (MQ=255)                                                                                       cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATcc                                                                 <  2:1114050/148‑1 (MQ=255)                                                                                          tAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGGAGATCATACCCGTCCGGGGAAACCACCCCGACCAGATAC‑AACCCTTCCCC‑TTTTTCCGTTGCTTCCGCCCGCGGTCTTT‑CCTTTGCCCCCCGCAACTCTGCTATCCAGctc                                                            >  2:673246/1‑150 (MQ=255)                                                                                          tAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGATTTTGGAGATTCAAACGGTCAGGGTAATCCCCCTCGACCCGATAC‑AGCCCCTCCCC‑TTTTTCCCCTGCCGCCCCCCGCGGTTTGT‑CCTTTGCCGCCAGCAACACTGCTTTCCAGctc                                                            >  2:412015/1‑150 (MQ=255)                                                                                            gTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATAC‑AGGCCCTCCGC‑TTTTTCCGTTGCTGCCGCCCGCGTTCTGT‑CCTTTGCCGCCCGCAACTCTGCTATCCACCCCTcc                                                         >  1:142655/1‑151 (MQ=255)                                                                                              ccGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCg                                                        <  2:567344/150‑1 (MQ=255)                                                                                               cGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGc                                                      <  1:28179/151‑1 (MQ=255)                                                                                               cGCCAGAAATAGCGGGCCCATTGGC‑GGCTTTTGAAGATCATACCGGGCAGGGGAATCCACCCCGGCCAGAAAC‑AGCCCCTCCCC‑TTTTTTCGCTGCGGCCGCCCACGTTCTGTCCCTTTGCC‑CCCGCGACCCTGCCTTCCAGCTCTcc                                                         >  1:414442/1‑148 (MQ=255)                                                                                                gCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCt                                                     <  1:581066/151‑1 (MQ=255)                                                                                                   aGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCTGGCTGGt                                                  <  1:66929/151‑1 (MQ=255)                                                                                                        tAGCGGGCCCATTGGC‑GGTTTTTGGAGATCATAACGGGCAGGGGAATCCACCGCGACCCGACAC‑AGCCCTTCCGC‑TTTTTGCGCTGCTGCCCCCCGCGGTCTGT‑CCTTTTCCGCCCGCAAATCTGCTATTCCGCTCTCCCGCTGGGTGTgg                                             >  1:1235504/1‑151 (MQ=255)                                                                                                        tAGCGGGCCCATTGGC‑GGTTTTGGAAAATCATACCGGGCAGGGGAATTCACCGCGGCCCGATAC‑AGCCCTTCCGC‑TTTTGCCGTCGCTGCCCCCCGCGTTCTGT‑CCCTTTCCGCCCGCCACTCTGCTCTCCAGCCCTcccccctggtgtgg                                             >  1:257428/1‑139 (MQ=255)                                                                                                          gCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGc                                           <  1:1231875/151‑1 (MQ=255)                                                                                                             ggCCCATTGGC‑GGTTTTGGGAAAACATAACCGGGAGGGGAAACCACCCCGACCCGATAC‑AGCCCTTCCCC‑TTTTGCCGTTGCTGCCGCCCGCGGTCTGT‑CCTTTTCCGCCAGCAACTCTGCTATCCAGCTCTCCGGGTGGGTGTGGGCAcc                                        >  1:498142/1‑151 (MQ=255)                                                                                                                    tGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTc                                  <  2:265445/150‑1 (MQ=255)                                                                                                                     ggC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTcc                                 <  2:481560/150‑1 (MQ=255)                                                                                                                       c‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc                              >  2:815455/1‑151 (MQ=255)                                                                                                                       c‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc                              <  1:453401/151‑1 (MQ=255)                                                                                                                         ggTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCa                             <  1:853446/151‑1 (MQ=255)                                                                                                                         ggTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCa                             <  1:497199/151‑1 (MQ=255)                                                                                                                          gTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAg                            <  2:797027/151‑1 (MQ=255)                                                                                                                            tttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGc                          >  1:194342/1‑151 (MQ=255)                                                                                                                               ggAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGc                       >  2:255861/1‑151 (MQ=255)                                                                                                                                 aaGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCg                     <  2:62669/151‑1 (MQ=255)                                                                                                                                      cATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACaa                 <  1:406540/150‑1 (MQ=255)                                                                                                                                      cATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAat                <  1:437074/151‑1 (MQ=255)                                                                                                                                       aTACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAata               <  2:721156/151‑1 (MQ=255)                                                                                                                                          ccGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTc            <  1:424432/151‑1 (MQ=255)                                                                                                                                           cGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTcc           <  2:654771/151‑1 (MQ=255)                                                                                                                                           cGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTcc           <  2:622972/151‑1 (MQ=255)                                                                                                                                           cGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTcc           <  2:913314/151‑1 (MQ=255)                                                                                                                                             gTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg         >  2:768420/1‑151 (MQ=255)                                                                                                                                                aGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc      >  1:1220421/1‑151 (MQ=255)                                                                                                                                                  ggTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCat    <  2:147652/151‑1 (MQ=255)                                                                                                                                                    tAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCatat  >  2:186230/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                          GTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGC‑GGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGC‑TTTTGCCGTTGCTGCCGCCAGCGTTCTGT‑CCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATAT  >  NC_000913/2434736‑2435032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑