Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	42.5%	106.8 / 62.1	94	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (8/46);  new base T (40/0);  total (48/46)
Fisher's exact test for biased strand distribution p-value = 6.70e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206289‑3206583                                                                                                                                                   |                                                                                                                                                      gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAg                                                                                                                                                    <  1:306786/150‑1 (MQ=255)     ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACaa                                                                                                                                                <  1:656679/150‑1 (MQ=255)       cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATa                                                                                                                                             <  2:535498/151‑1 (MQ=255)       cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATa                                                                                                                                             <  1:1230304/151‑1 (MQ=255)       cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACaaa                                                                                                                                               >  2:1349790/1‑149 (MQ=255)        gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc                                                                                                                                            <  2:1753998/151‑1 (MQ=255)        gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc                                                                                                                                            <  1:673376/151‑1 (MQ=255)         cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTAATTTTTTTAGACaaaaa                                                                                                                                             >  2:1733026/1‑147 (MQ=255)         cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACaaa                                                                                                                                               >  2:800389/1‑147 (MQ=255)          aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑a                                                                                                                                          >  2:99337/1‑150 (MQ=255)            aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAaaaaa‑aaa                                                                                                                                        >  2:738725/1‑150 (MQ=255)             aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCTCACGAAAAC‑aaaa                                                                                                                                       >  1:553749/1‑150 (MQ=255)              cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAcaaaaac‑aaaaa                                                                                                                                      >  1:261248/1‑150 (MQ=255)                 tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAA‑AAAAAATgg                                                                                                                                  >  1:123680/1‑151 (MQ=255)                 tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAACttg                                                                                                                                  >  1:1683702/1‑149 (MQ=255)                 tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTg                                                                                                                                   <  2:1007584/150‑1 (MQ=255)                  gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTgg                                                                                                                                  <  2:414158/150‑1 (MQ=255)                    cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGt                                                                                                                               <  1:963241/151‑1 (MQ=255)                       tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCGCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTg                                                                                                                             <  2:1488945/150‑1 (MQ=255)                       tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTg                                                                                                                             <  1:840813/150‑1 (MQ=255)                          cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAA‑AAAAACTGGAGGTgtcct                                                                                                                         >  2:880439/1‑147 (MQ=255)                               ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCatgat                                                                                                                      <  2:291540/149‑1 (MQ=255)                                tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGTATTTTTTCAGACAAATAC‑AAAAAAAGGAGTTGCCatgat                                                                                                                      >  1:659416/1‑148 (MQ=255)                                   cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAAATGGAGGTGGCATGATGAggg                                                                                                                 >  1:1663697/1‑148 (MQ=255)                                    gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTTAGAAAAAAAA‑AAAAAATGGAGTTGGCATGATGAGCg                                                                                                                 >  2:1672052/1‑149 (MQ=255)                                     cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGaaa                                                                                                              <  1:252130/151‑1 (MQ=255)                                     cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGaaa                                                                                                              <  2:1341575/151‑1 (MQ=255)                                         attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGtaa                                                                                                          <  1:1345474/151‑1 (MQ=255)                                            cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGtaataa                                                                                                       <  2:721938/151‑1 (MQ=255)                                               tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAATAA‑AAAAAATGGAGTTGTCCTGATGGGCGAAAGgaa                                                                                                          >  2:459930/1‑145 (MQ=255)                                                 ttATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAC‑AAAAAAAGGAGGTGGCATGGTGAGGGAAAGtaataaaaaa                                                                                                   >  2:1577424/1‑146 (MQ=255)                                                   aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAA‑AAAAAATGGAGGTGCCATGATGAGCGGAAGGAAAAAacaaca                                                                                                 >  1:97170/1‑150 (MQ=255)                                                   aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAg                                                                                                <  2:53523/151‑1 (MQ=255)                                                    ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAA‑AAAAAATGGAGGTGGCCTGATGAGGGaaaagaaaaaaaaaaa                                                                                                 >  1:82716/1‑136 (MQ=255)                                                     tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                               <  2:252883/150‑1 (MQ=255)                                                          cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCGACAAAAAA‑AAAAACAGGGGGTTCCCTGGTGAGCGAAAAGAATAAACaaaaaggaggg                                                                                          >  1:934799/1‑141 (MQ=255)                                                             tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt                                                                                       <  2:1762080/150‑1 (MQ=255)                                                              gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                                      <  1:113691/150‑1 (MQ=255)                                                               aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAC‑AAAAAATGGGGTTGCCATGATGGGGGAAAAGAAAAAacaacaaggaggggata                                                                                      >  2:1382941/1‑138 (MQ=255)                                                                 tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGt                                                                                   <  1:434741/150‑1 (MQ=255)                                                                   aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGa                                                                                <  1:151609/151‑1 (MQ=255)                                                                     gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa                                                                              <  1:994545/151‑1 (MQ=255)                                                                     gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAA‑AAAAAATGGAGGTGCCATGATGAGGGAAAGGAATAAGAAAAAAGCAGGGAATAAGtt                                                                                  >  1:280281/1‑147 (MQ=255)                                                                        cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAC‑AAAAAATGGAGGTGGCATGATGAGCGAAAGGAATAAACAACAAGCAGGGAATAAATTGACa                                                                              >  1:431742/1‑148 (MQ=255)                                                                          gTTAATTACATATTCACGGTGGC‑AAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                         <  2:1273115/150‑1 (MQ=255)                                                                            tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAA‑AAAAAATGGAGGTGCCATGATGAGGGGAAGAAAAAAACAAAAGGCAGTGGATAAATTGaaa                                                                              >  1:134814/1‑142 (MQ=255)                                                                             aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCACAAAAAAAA‑AAAAACAGGGGTTGGCAAGATGAGCGCAAGAAATAAAAAAAAAGCAGTGAAAAAATTGATAGAGAGTg                                                                       >  2:1214560/1‑150 (MQ=255)                                                                                 aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGc                                                                   <  1:1496481/150‑1 (MQ=255)                                                                                   atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGGTGCCATGATGAGCGAAAGAAAAAAACAACAGGCAGTGAATAAGATGACAGAGATTGTCGCTa                                                                 >  1:902667/1‑150 (MQ=255)                                                                                   atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTaa                                                                <  2:769486/151‑1 (MQ=255)                                                                                   atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAC‑AAAAACTTGAGTTGGCCTGATGAGCGGAAAGAATAAAAAACAAACAGAGAAAAAAATTACAGAGATTGTTGCTa                                                                 >  2:842378/1‑150 (MQ=255)                                                                                    tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAA‑AAAAAATGGAGGTGCCCTGATGAGCGAAAGTAATAAACAAAAGGCAGGGAATAAATTTAAAGAGATTGTTGCTaa                                                                >  1:822579/1‑150 (MQ=255)                                                                                     atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAC‑AAAAAATGGAGTTGCCATGATGAGGGAAAGTAATAAACAAAAAGCAGGGAATAAATTTACAGAGATTTTTGCTaa                                                                >  2:1557430/1‑149 (MQ=255)                                                                                     atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAAc                                                               <  1:440624/150‑1 (MQ=255)                                                                                       tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAC‑AAAAAATTGAGTTGGCCTGATGAGCGAAAAGAATAAACAAAAAGCAGTGAATAAAATGACAGAGAATGTCGCTAAAttt                                                            >  2:471766/1‑151 (MQ=255)                                                                                        cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAACTGGAGTTGGCATGATGAGCGAAAGTAATAAACAACAAGCAGGGAATAAGTTGACAGAGATTGTCGCTAACttt                                                            >  1:650796/1‑150 (MQ=255)                                                                                         aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAc                                                          <  2:1756444/151‑1 (MQ=255)                                                                                          cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCACAAAAAAAA‑AAAAAATTGGGTTGCCCTGATGAGCGGAAATAAAAAACAAAAAGGAGGGAATAAATTGAAAGAGATTGTCGCCAACttttac                                                         >  1:1005709/1‑148 (MQ=255)                                                                                          cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACCAAAAC‑AAAAACAGGAGTTGTCCTGATGAGGGGAAAGAATAAACAACAAGGAGTGGATAAATTGAAAGAGAATTTCGCTAACTTTAcc                                                         >  1:1691033/1‑151 (MQ=255)                                                                                          cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAcc                                                         <  2:1287966/151‑1 (MQ=255)                                                                                           ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCg                                                        <  1:1465800/151‑1 (MQ=255)                                                                                             tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAC‑AAAAAATGGAGGTGGCATGATGAGGGAAAGGAATAAACAAAAAGCAGGGAATAAGTTGACAGAGATTGTCGCTAAATTTACCGcc                                                      >  1:368770/1‑151 (MQ=255)                                                                                             tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAGACAAATAA‑AAAAAATGGAGTTGGCATGATGAGCGGAAGGAATAAACAAAAAGCAGTGAATAAATTGACAGAGATTGTCGCTAACTTTACCGcc                                                      >  2:482432/1‑151 (MQ=255)                                                                                              ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa                                                     <  1:1126813/151‑1 (MQ=255)                                                                                                 aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATg                                                   <  2:896452/150‑1 (MQ=255)                                                                                                   aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAAACAAAAAA‑AAAAAATGGAGGTGCCATGATGAGGGAAAAGAAAAAACAAAAAGCAGTGAAAAAGATGAAAGAGGTTGTTGCTAAATTTTCCGCCATGAtt                                                >  2:1235990/1‑151 (MQ=255)                                                                                                    aaaTATAAACCCACATTTTTAGTGGTATTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGCCGCTAACTTTACCGCCATGAttt                                               <  2:494759/151‑1 (MQ=255)                                                                                                    aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGATTTTTTCCGACAAAAACAAAAAATTGGGGTTGCCA‑GATGAGGGACAGGAATAAACAAAAGGCAGGGAATAAGATGAAAGAGATTTTTGCTTACCTTATCGCCACGActt                                               >  2:1302090/1‑151 (MQ=255)                                                                                                    aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATAC‑AAAAACTGGAGGTGCCATGATGAGCGGAAGTAATAAACAACAGGCAGGGAATAAAGTGACAGAGATTGTTGCTAAATTTACCGCCCTGAttt                                               >  1:19019/1‑151 (MQ=255)                                                                                                      atatAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAAAAGGAGGTGCCATGATGAGAGAAAGGAATAAAAAAAAGGGAGGGAATAAATTTACAGAGATTGTTGCTCAATTTTCCGCCATGAttttt                                             >  1:1274468/1‑149 (MQ=255)                                                                                                      atatAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAACAAAATAA‑AAAAAAAGGGATTGGCCTGATGAGGGGAAGGAAAAAACAAAAAGGCGTGAAAAAATTGACAGAGAATGTCGCTAACCTTACCCCCCTGATTTCt                                             >  2:159855/1‑151 (MQ=255)                                                                                                        ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTAc                                           <  2:1640386/151‑1 (MQ=255)                                                                                                          aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAAATGGAGGTGCCATGATGAGCGGAAGAAATAAACAAAAAGCAGGGAATAAAATGACAGAGATTGTCGCTAAATTTACCGCCCTGATTTTTAccc                                         >  1:241422/1‑151 (MQ=255)                                                                                                           aaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAA‑AAAAAATTGGGGTGCCATGGTGAGGGGAAATAATAAACAACAAGCAGGGGATAAGATGACAGAGAATGTCGGTCAATTTTCCCCCCTGATTTCTAccc                                         >  1:271948/1‑150 (MQ=255)                                                                                                              ccACATTTTTAGTGGTATTTTGTGGCGGTAAATTTTCCCAGACAAAAAA‑AAAAACTGGAGTTGCCAGGATGAGCGAAAGAAATAAGCAACAGGCAGTGAATAAGTGGAAAGAGTTTGTCGCAAACTTTACCGCCATGATTTCTACCCGAAt                                     <  2:1422008/151‑1 (MQ=255)                                                                                                                     tttAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAAA‑AAAAAATGGGGGTGCCATGATGAGGGGAAGGAATAAGCAACAGGGAGGGAAAAAATTGAAAGAGATTGTCGCCAACCTTTACGCCCTGATTTTTAACCGAAAGcc                                  >  1:1621018/1‑147 (MQ=255)                                                                                                                      ttAGTGGTAGTTTGGGGCGGTGAATTTTTCCAGACAAATAA‑AAAACCTGGAGTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTAAATAAGTGGACAGAGATGGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCtgat                              <  2:279107/150‑1 (MQ=255)                                                                                                                          tGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCATGAGGAGCAAAAGTAAAAACCAACAGGCAGTAAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGAc                           <  2:1343648/149‑1 (MQ=255)                                                                                                                           ggTAGTTTGTGGCGGTGAATTTTTCAAGACAAATAC‑AAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAACCAACAGGCAGTAAATAATTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                         <  2:1581972/150‑1 (MQ=255)                                                                                                                                tttGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCAATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtgg                       <  2:612522/147‑1 (MQ=255)                                                                                                                                   gtgGCGGTGATTTTTTCAAGACAAATAA‑AAAAACGGGAGTTGCAATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt                  <  2:300850/149‑1 (MQ=255)                                                                                                                                    tgGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                >  2:918663/1‑150 (MQ=255)                                                                                                                                    gtgggggtaaTTTTTCCAGAAAAATAC‑AAAATCTGGAGTTGCCATGATGAGTGAAAGAAATAACCAACAGGAATTAAATAAGTTGACAGATTTTGTCGCAATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt                  <  2:1589759/140‑1 (MQ=255)                                                                                                                                     ggCGGTGAATTTTTCCAGAAAAATAA‑AAAACCTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc              <  1:800427/151‑1 (MQ=255)                                                                                                                                       cGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt             >  1:1670232/1‑150 (MQ=255)                                                                                                                                        ggTGAATTTTCCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa            <  1:738758/150‑1 (MQ=255)                                                                                                                                        ggTAAATTTTACCAGAAAAAAAC‑AAAAACGGGAGTTGCCATGATGAGCAAAAGAAAAAAGCAACAGGCAGTGAATAAGTGGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa            <  2:350639/150‑1 (MQ=255)                                                                                                                                         gTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa           >  1:1124638/1‑150 (MQ=255)                                                                                                                                         gTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa           >  2:1061618/1‑150 (MQ=255)                                                                                                                                            aaTTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc        >  1:1677170/1‑150 (MQ=255)                                                                                                                                             aTTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc        >  2:1226045/1‑149 (MQ=255)                                                                                                                                              tttttCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc     >  2:425650/1‑151 (MQ=255)                                                                                                                                              tttttCCAGAAAAATAC‑AAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAg      <  2:298624/150‑1 (MQ=255)                                                                                                                                               ttttCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc     <  1:335420/150‑1 (MQ=255)                                                                                                                                                   ccAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  >  2:1766504/1‑149 (MQ=255)                                                                                                                                                   ccAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  >  2:1499320/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                      GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206289‑3206583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑