| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,151,689 | 0 | G | T | 64.7% | 12.8 / 6.5 | 35 | intergenic (+43/+22) | yegJ/yegK | uncharacterized protein/ser/thr phosphatase‑related protein |
| Reads supporting (aligned to +/- strand): ref base G (9/3); new base T (14/8); total (23/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01 | |||||||||||
GCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAA‑TTG‑TTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAG > NC_000913/2151567‑2151839 | gggtttatcctgttGATCTTTTATTGGTCCCCTCTAAAAAAGAAAAAGCCGACGAGATAAGAGCAAAATTAGTTCGCTAATCAGCCTTTATTTTTTTTTTTAAAA‑CTGGCGGTTGAA‑TTG‑TTGTCTT‑CAACCGCCATGAGAAactcactc < 1:131338/138‑1 (MQ=255) ggTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAA‑TTG‑TTTTCTT‑CCAACGCCATGAGAAACTCAATCTg > 1:286579/1‑150 (MQ=255) ggTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAA‑TTG‑TTGTCTT‑CAACCGCCATGAGAAACTCACTCTg > 1:93806/1‑150 (MQ=255) gTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAA‑TTG‑TTTTCTT‑TAACCGCCATGGGAAAATCACTCTGc > 2:102193/1‑150 (MQ=255) gTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAA‑TTG‑TTTTCTT‑CAACCCCCCTGAGAAAATCACTCTGc > 2:253057/1‑150 (MQ=255) ccATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAA‑TTG‑TTGTCCT‑TAAACCCCCTGAGAAACTCACCCTGccccccg > 2:100006/1‑146 (MQ=255) aTCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAA‑TTT‑TTTTCTT‑CAACCGCCCTGAGAAAATACCTCTTCCCCCCGCGCCAGt > 1:241593/1‑151 (MQ=255) aTCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAA‑TTT‑TTTTCTT‑CAACCGCCATTAGGAACTCACTCTGCCCACAGCGCCCGt > 1:401672/1‑151 (MQ=255) tcttttATTGCTCCCCTTACAAAAAGAAGAGGCCGACGAGCTAAGAGAAAATTTGTTTCGTTAATCAGCCTTTATTTTTTTTGTTTAAC‑CTGGCGTTTAAT‑TTT‑TTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTg < 1:263105/148‑1 (MQ=255) tCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAA‑TTT‑TTTTTTT‑CAACCCCCCTGAGAAACTCACCCTGGCCCCAGCGCCaagg > 2:269141/1‑148 (MQ=255) aTTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAA‑TTG‑TTGTCTT‑CAACCGCCATGGGAAACTCACTCTGCCCCCAGCGCCAGTGCCAACg > 2:278486/1‑151 (MQ=255) ttGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTTTA‑TTT‑TTGTCTT‑CAACCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGt > 1:405956/1‑151 (MQ=255) ttGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTTAA‑TTG‑TTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCCCAGCGCCCGTGCCAACGt > 2:392900/1‑151 (MQ=255) ggTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAA‑TTG‑TTGTCTT‑CAACCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGttt > 1:97001/1‑151 (MQ=255) ccTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAA‑TTG‑TTTTTTT‑CAACCGCCATGAGAAACTCACTCTGCCCCCAGCGCCCGTGCCCACGTTTTttca > 2:376484/1‑151 (MQ=255) ccTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAA‑TTG‑TTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCCGTGCCAACGTTTTatca > 2:69222/1‑151 (MQ=255) aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAA‑TTG‑TTGTCTT‑TAACCCCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAAAGTTTTttcatca > 2:141541/1‑151 (MQ=255) aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGATGAA‑TTG‑TTGTCTT‑CAACCCCCATGAGAAAATCACCCTGCCCACAGCCCCAGTGGCCAAGGTTTatcaata > 1:144888/1‑148 (MQ=255) caaaaaGAAAAAGCGTACGAGCAAAGAGAAAAATTAGTCCGCAATACCGCCTTTATATTTATGGTTAAAA‑CGGGGGTTAAAA‑TTG‑TTTTCTC‑AACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcatcatc < 1:142958/149‑1 (MQ=255) aaaaagaagCCGAAGAGTTAAGAGCAAATTTGTTTCTCTATCCCGCCTTTATATTTTTTGTTTAAC‑CGGGCGTTGAAA‑TTG‑TTTTTTC‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTa < 2:206525/148‑1 (MQ=255) aagaacCCGACGAGCAAAGAAAAAAATTTTTTCTTAAATCAGCCTTTATTTTTTTTGTTTAAA‑CGGGCGGTTAAA‑TTT‑TTTTCTT‑AAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTAc < 2:276003/149‑1 (MQ=255) aGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTTAA‑ATG‑TTTTCTT‑TCACCGCCCTGAGAAACCCCCTCTGCCCCCAGCGCCCGTGCCCACGTTTTTTCCTCATCCCTACGCTCGTTTAcccc > 2:376289/1‑148 (MQ=255) gagCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAA‑TTG‑TTTTCTT‑CAACCCCCATGAGAAACTCACTCTTCCCACAGCGCCAGTGCCAAAGTTTTTTCATCCTCCGTACCCTCGTTTTCCCCCggg > 2:381229/1‑148 (MQ=255) gCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAA‑TTG‑TTTTTTT‑CAACCGCCATGAGAAACTTACTCTGCCCACAGCGCCAGGGCCAACGTTTTATCATCATCCGTACGCCCGTTTACCGCCGGGCTGGa > 1:192584/1‑151 (MQ=255) cAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTTAA‑TTT‑TTTTCTT‑CAACCCCCATGAGAAAATCACTCCGCCCACAACGCCAGTGCCAACGTTTTATCATCATCCCTACCCTCGTTTTCCCCCGGGCTGGa > 1:181065/1‑150 (MQ=255) aaaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAA‑TTG‑TTTTCTT‑CAACCGCCATGAGAAAATTACTCTGCCCACAGGGCCAGTGCCAAAGTTTTATCTTCATACGTAAGCTCCCTTACCGCCCGGCCGGACaa > 1:129556/1‑151 (MQ=255) cTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAA‑TTG‑TTTTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGGCAAAAAATGCTTTa > 1:323399/1‑151 (MQ=255) aacccccctttATTTTTATTG‑TTAAA‑CCGGGGGTTGAATTTT‑TTTTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAAc < 2:397831/142‑1 (MQ=255) aaacacccGTTATATTTTTTG‑TTAAA‑CGGGGCGGTGAA‑TTGTTTAGTTT‑AAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAAc < 2:301361/145‑1 (MQ=255) aacAGCCTTTATTTTTTTTTTTAAAA‑CGGGCGTTTAAA‑TTT‑TTTTTTC‑CACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACa < 2:201233/149‑1 (MQ=255) ccAGCCGTTATTTTTATTTTTAAAC‑CTGGCGTTTAAT‑TTT‑TTTTCTC‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACaa < 1:89018/150‑1 (MQ=255) ttttGTTAAAC‑CGGGGGTTTAAA‑TTG‑TTTTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTGCCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAAt < 2:219330/149‑1 (MQ=255) tttttttAAACCTGGCGGTAAAG‑TTT‑TTGTCTC‑AAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAAt < 1:187640/147‑1 (MQ=255) gTTTAAA‑CTGGCGGTTGAA‑TTG‑TTTTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACCGCGCCAGTGCCAACGTTTTATTATCATCCGTACGCTCGTTTACCGCCGGGCCGGAGAAAAACTGCTTTAACAATTCTGGTAATAAATCGAg > 1:381247/1‑151 (MQ=255) ttAAA‑CCTGGGGTTTAA‑TTT‑TTTTTTTCCAACCGCCATGAGAAACTCGCTCTGCCCTCAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgt < 2:129584/151‑1 (MQ=255) gtCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAg > 1:288675/1‑151 (MQ=255) | GCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAA‑TTG‑TTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAG > NC_000913/2151567‑2151839 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |