| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 827,507 | 0 | T | G | 45.1% | 61.7 / 11.2 | 51 | D492A (GAC→GCC) | ybhF | putative ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base T (9/19); new base G (23/0); total (32/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.62e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACA‑GGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCG > NC_000913/827369‑827655 | aTTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAg < 1:1012848/151‑1 (MQ=255) tAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTc < 1:907618/151‑1 (MQ=255) gTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCCACGCCCGGCGTCGGTttgtcccga > 2:317726/1‑147 (MQ=255) acacCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGGGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCCACGCCGGGAGTggggttttccggaaa > 1:169091/1‑139 (MQ=255) cacCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGAAGTCGGTTCGGCGAGaaaa > 1:78974/1‑150 (MQ=255) cacCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCGGGTCGTCGCGaaaa > 1:357343/1‑150 (MQ=255) cAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCa < 2:1148356/151‑1 (MQ=255) atatTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGTGAACGGCGGGAGGCGGGTTGTCGAGACAAAGAAAATCCGGtgcatgcac > 2:740697/1‑149 (MQ=255) atatTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCCCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAAAAGAATGTCCGGGTCATGCAt > 1:1099745/1‑150 (MQ=255) tttCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGTGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAg < 2:1120046/150‑1 (MQ=255) tCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAAAGCCCGAAGTCGGTTCGGCGGGAAAAAGAATGGCCGGTTCATGCATCAGCGAAAA‑gggc > 1:242523/1‑148 (MQ=255) ccATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGTCGGTTCGTCGAGAAACAGAAAGTCCGGGTTATGCATCAGCGGAAA‑Ggcccg > 1:310708/1‑149 (MQ=255) tAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACA‑Ggccagcgc < 2:824295/151‑1 (MQ=255) tAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACA‑Ggccagcgc < 1:343253/151‑1 (MQ=255) tAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATGCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACA‑Ggccagcgc < 2:564184/151‑1 (MQ=255) tAAAGAGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGTAAACGCCCGGAGTCCGTTTGTCGAGGAAAAGAATGTTCGGGTCTTGGATTAGCGAAAA‑Agcccgcg > 1:575037/1‑150 (MQ=255) tgACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACA‑GGCCAGGGCCAGCCGCTGttt < 1:932720/151‑1 (MQ=255) gACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAAAGCCGGAAGTCGGGTCGTCGAGAAAAAGAATGTCCGGGTCCTGCATCAGCGAACA‑GGCCAGCGCCCGCCCCCGTTTa > 1:244453/1‑151 (MQ=255) gACCATCACCGTGACGCCTTCCTCTACCATGCTGTTGATTTGCAGCCAAAATTCACGGCGGGCGGGGGGGTTCATGCCCGGACTCGCGTCGTTTGGGAACACCAAGTGCGGTTCAATCATCAGCGCACA‑AGCCAGCGCCAGcccaggtttt > 2:687777/1‑143 (MQ=255) aacACCGTGACGCCTGTCTCTACCATGCGGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATACATCAGCGAACA‑GGCCAGCGCCAGCCGCTGTTTAAAAc < 2:61697/149‑1 (MQ=255) aCCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGGTCGTCGAGAAAAAGAATGTGCGGGTCATGCCTCAGCGAAAA‑GGCCAGCGCCCGCCCCCGTTTTAAACCTa > 2:926446/1‑151 (MQ=255) ccGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGCGTGGGGGGCCACGCCCGGAGGCGGTTCGTCGGGAAACAGAAAGTCCGGTTCATGGATCAGCGAACA‑AGCCCGCGCCAACCGCCGTCTTTAACCtta > 2:253589/1‑149 (MQ=255) aCGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACA‑GGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGc < 1:764275/150‑1 (MQ=255) aCGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACA‑GGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGc < 1:838906/150‑1 (MQ=255) ccTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAAGCCGGGAGTGGAGTCGTCCAGAAACAGAAAGTTCGGGTTATGCATCAACGAACA‑GGCCAACGGCCGACGCTGTTTAAAACCTAATGTCCGtt > 2:818616/1‑151 (MQ=255) ccTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAAGCCGGAAGTCGGGTCGTCGGGAAAAAGAATGTCCGGGTCATGCCTCAGCGAACA‑GGCCCGCGCCCGCCGCCGTTTTAAAACTAATGGGAggt > 1:371503/1‑149 (MQ=255) ccTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCCGGAGTCGGTTCGTCGAGAAACAGAAAGTCCGGGTCATTCATCAACGAAAA‑AGCCAGCGCCCGGCCCTGTTTTAAAACCAATTGCAggt > 1:121796/1‑149 (MQ=255) tttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGCAACGCCTGGAGTCGGTTCGTCGAGGAAAAGAATGTCCCGGTCCTGGCTCCGCGAAAA‑GGCCCGCGCCCGCCGCCGTTTAAAACCCAAACGCAggtta > 2:75150/1‑147 (MQ=255) tttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAAAGCCGGAAGTTGGGTTTTCGAGAAAAAGAATGTCCGGGTCATGTCTCCGCGAACA‑AGCCAGCGCCAGCCGCTGTTTTAAAACTAATGGccggt > 1:540062/1‑145 (MQ=255) tttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGCGGGGGGGGCGACGCCCGAAGCCCGTTCTTCCGGAAAAAGAGTGGCGGGTGCCTCCATCTGCGAACA‑GGCCCGCGCCCCCCGCCGTTCAAAACCTAATGCCAGtgtt > 2:82671/1‑148 (MQ=255) tctcTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGTAACGCCCGAAGGGGGTTTCGCTAGAAAAAGAGTGTTCGGGTCATTTGACAGCGGAAA‑GGACCGCGCGAGCCCGCGTTTTTAACACAATGGCAgcttatg > 2:766095/1‑145 (MQ=255) tctcTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAAAGCCGGAAGGCGGTTCGTCGAGAAAAAGAATGTCCGGGTCATGCATCAGCGAAAA‑AGCCAGCGCCAGCCGCCGTTTAAAAACTAATGGCAggttat > 1:198089/1‑145 (MQ=255) ctACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGTCAACGCCCGAAGTCGGGTCGTCGAGAAACAGAAAGTCCGGGTCATGCAACAGCGAAAA‑GGCCCGCGCCCGCCCCTGGTTAAAAACTAAAGGGAGGTCATCggg > 1:1066461/1‑150 (MQ=255) ctACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAAGGGGGCAACTCGGGCCCACGGTTCCGTGGGAAACAGAAAGTTCGGTTAAAGCACCAGCCAACA‑CGCCAGGCCCCACCGCTTTTTTAAACCCAATCGGAGTTTAtaagt > 1:822920/1‑147 (MQ=255) aCCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTTGTCGGGAAAAAGAATGTCCGGTTCATGCCTCAGCGAACA‑GGCCCGCGCCCCCCCCCGTTTTAAACCCCAAGGCCGTTCATCCGTgg > 1:572381/1‑151 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAAAGCCGGAAGGCGGGTCGTCGAGAAAAAAAAAGTCCGGGTCATGCATCAGCGAACA‑GGCCAGCGCCAGCCGCTGTTTAAAAACCAATGGGCGGTCATCGGTGGCgg > 2:523805/1‑150 (MQ=255) tGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGGCCGGGTCATGCATCAGCGAACA‑GGCCAGCGCCAGCCGCCGTTTAAAACCCAAAGGCAggttctcggggggggg > 2:483212/1‑136 (MQ=255) cTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAAACCCGGGAATCGGGGTGGGGGAAAAAAGAAAGGCCGGTTCATGGATTAGCAAACACGCCCAGGGCCA‑CCGCCGTTTTAAAACTAAAGGCAGTTTAACGgggggggggg > 1:586747/1‑151 (MQ=255) cTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGTCAACGCCGGAAGGCGGTTTGTCGAGAAAAAGAATGTCCGGGTCATGCATCAACGAACA‑GGCCCGCGCCCGCCGCTGGTTTAAACCTAAAGGGAGGTTATTGGGGGCGgggg > 1:1141383/1‑151 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGTAACGCCGGGAGGCGGGTTGTCGAGAAAAAAAAAGTCCGGGTCATGCAACAGCGAAAA‑GGCCCGCGCCCGCCGCTGTTTTAAACCCAAAGGCCGGTCAAcgggggcggggggg > 1:146492/1‑149 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACA‑GGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGAt < 1:842453/151‑1 (MQ=255) tgCAGCCAAAATTCACGGCGGGTGAGGGTGTCAACGCCGGAAGTCGGTTCGTCGATAACCAGAATGTCCGGTTCATGCATCAGCGACCA‑GGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTTGCGTGGGAGGCGATAc < 2:801238/151‑1 (MQ=255) tgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACA‑GGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATAc < 1:199515/151‑1 (MQ=255) tgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACA‑GGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATAc < 2:318577/151‑1 (MQ=255) ccAAAATTCACGGCGGGTGAGGGGGTCACCGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACA‑GGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTc < 2:614911/151‑1 (MQ=255) ccAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACA‑GGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTc < 2:284696/151‑1 (MQ=255) gggggTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACA‑GGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGCTACTTTTCAGGCCGAACGCCTCGCTCAt > 2:1014452/1‑151 (MQ=255) ggggTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACA‑GGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGACACTTTTCAGGCCGAACGCCTCGCTCATg > 2:353977/1‑151 (MQ=255) gggTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACA‑GGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATg < 1:1100380/150‑1 (MQ=255) gggTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACA‑GGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATg < 1:715007/150‑1 (MQ=255) ggTCAACCCCTGATGTCGCTTCGACGAGAAACCGAATGTCCGGTTCATGCATCTGCGAACA‑GGCCAGCCCCAGCCGCTGTTTAAAACCTAATGGCAGTTTATTGGTGACATCGGCGGCGATACTTTCGAGGGCCAACGACTCGCTCATGCg > 2:327776/1‑151 (MQ=255) | ATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACA‑GGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCG > NC_000913/827369‑827655 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |