BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,379,266	(C)_7→8	coding (732/1128 nt)	zapE ←	divisome ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,379,259	1	.	C	88.9%	259.8 / 24.2	82	coding (739/1128 nt)	zapE	divisome ATPase
Reads supporting (aligned to +/- strand): ref base . (1/8); new base C (40/32); total (42/40)
Fisher's exact test for biased strand distribution p-value = 1.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAGACGTGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTACATCACAATGCTGTTT  >  NC_000913/3379115‑3379403

agagACGTGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑ccccc                                                                                                                                              >  1:734025/1‑150 (MQ=255)

  agaCGTGAGGGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGCCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑cccccc‑c                                                                                                                                           <  2:39918/150‑1 (MQ=255)

  agaCGTGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑cccccc‑c                                                                                                                                           >  2:115429/1‑150 (MQ=255)

     cGTGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑cccccc‑ccgcc                                                                                                                                       <  1:978968/151‑5 (MQ=255)

           agCACAATATAGTCATGCTGCCTGGGGGCGTCGACGCCCAGCGTATAAAAAGAGACCGCCGGCGTCTGGTTCTGGGCGCCCTTTGTTGCAAATGCCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCTTT‑TC‑CCCCCC‑CGCCAgcgc                                                                                                                                   <  2:419907/150‑1 (MQ=255)

            gcgcAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCCCGTT‑TC‑CCCCCC‑CCCCAGCGCCa                                                                                                                                 >  1:1161187/1‑151 (MQ=255)

               cAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAAc                                                                                                                               >  1:87317/1‑150 (MQ=255)

               cAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAAc                                                                                                                               >  1:391558/1‑150 (MQ=255)

                 atatatTCATGCTGCCGGGGGGGGCCGCCGCACGGCGTAGAAAAAGGGCCCGCCGGCGTTTGTTTCCCGCCGCCCTTTTTTGCTATTGCCCGTTGTTTGATTTCAAACGTGGGTGATTTTTCTCTTTCC‑‑CCCCCC‑CGCCAGCGCCAACCAt                                                                                                                            <  1:461262/151‑1 (MQ=255)

                 atatAGTCATGCTGCCTCCGGCCGTCGACGCACATCTTTCTAAAAGATACCTCCAGCGTCTGGTTCTCGACGTCCATTGTCCCTAATGGCCCATGGCAGATTTCTAACGTCGGGTAACTTTCTCGTT‑T‑‑CCCCCCTCGCCAGCGccatccat                                                                                                                            >  2:807338/1‑151 (MQ=255)

                 atatAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCAt                                                                                                                            >  2:401685/1‑151 (MQ=255)

                 atatAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCAt                                                                                                                            >  2:350734/1‑151 (MQ=255)

                 atatAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCAt                                                                                                                            >  1:720597/1‑151 (MQ=255)

                 atatAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCAt                                                                                                                            >  1:558631/1‑151 (MQ=255)

                 atatAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCAt                                                                                                                            >  1:1117032/1‑151 (MQ=255)

                 atatAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCAt                                                                                                                            >  1:1057078/1‑151 (MQ=255)

                 atatAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCAt                                                                                                                            >  1:1132215/1‑151 (MQ=255)

                    taGTCATGCTGCCTGCGGGCGTCGACGCACAGCGTAGTAAAGGAGACCGCCAGCGTCTGGTTCGCGACGCCCTTTGTTGCTAATGGCCGATGGTTGATTTCTACCGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGt                                                                                                                         <  2:1056686/151‑1 (MQ=255)

                    taGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGt                                                                                                                         >  1:563181/1‑151 (MQ=255)

                      ttCATGCTGACTGGGGGCGTCGCCGCACAGCGTAGTAAAAGAGACCGCCAGCTTCTGGTTCTCGACGCCCATTTTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGttt                                                                                                                       <  1:994428/150‑1 (MQ=255)

                      gTCATGCTGACTGCGGGCGCCGCCGCCCAGGGTAGAAAAAGAGCCCGCCGGCGTCGGGTTCTCAACGCCATTTTTTACAAAGGCCCGATGGTTGATTTCTAACGGCGGTGAATTTTCCCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGtt                                                                                                                        <  2:231109/150‑1 (MQ=255)

                      ataaTGCTGACGGGGGGCGTCGACGCACAGGGTAGTAAAAGAGACCGCCAGCGTCGGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGttt                                                                                                                       <  2:267930/148‑1 (MQ=255)

                       gcaggttGACTGCGGGCGTCGGCCCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGTTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCTTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGttt                                                                                                                       <  1:433630/144‑1 (MQ=255)

                       gcATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGCCCGCAGGCGTCTGTTTCTCGACGCCCATTGTTGCTAATGGCCGATGTTTGATTTCTAACGTCGGTGAATTTTCTCGTTAC‑‑CCCCCC‑CGCCAGCGCCAACCATAGtt                                                                                                                        <  1:110706/148‑1 (MQ=255)

                       cttttactacgctgtgCGTCGACGCCCAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTa                                                                                                                      >  2:99466/16‑151 (MQ=255)

                        cATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTAt                                                                                                                     >  1:424348/1‑151 (MQ=255)

                         aTGCTGACTGCGGGGGGCGACGCCCAGGGTAGAAAAAGAGCCCGCGAGTGTCTGGTTCTCGACGCCCATTGTTGCCAATGGCCGATGGTGGATTTCTACCGTCGGTGAATTTTCTCTTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATc                                                                                                                    <  1:401799/151‑1 (MQ=255)

                         aTGCTGACTGCGGGCTTCGACGCACAGCTTAGTAAAAGAGCCCGCCCGCGTCTGGTTCTCGCCGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACTTGGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATc                                                                                                                    <  2:772941/151‑1 (MQ=255)

                         aTGCGGACGGCGGGAGTCGACGCAGAGTGTAGTAAAAGCGACCGCCGGCGTTTGGTTCTCCACGCCCATTGTTGATAATGGCCGATGGTTGATTTCAAACTTGGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGGTTATc                                                                                                                    <  2:385038/151‑1 (MQ=255)

                          tgctgcCGGCGGGCGCCGACGCACAGCGTAGTAAAAGAGTCCGCCAGCGTCTGGTTTTCGACGCCAATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATcc                                                                                                                   <  1:302441/151‑1 (MQ=255)

                           gCTGACTGCGGGCGGGGCCGAACAGGTTAGAAAAAGAGCCCGCCAGGGTCTGGTTCTCGACGCCGATTGTTGCTAAGGCCGGATGGTTGATTTCTAACGTCGGTGAATTTTCTCTTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATcc                                                                                                                   <  2:1237196/150‑1 (MQ=255)

                            ctgcctgcGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTTTGGTTCTCGACGCCCATTTTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCa                                                                                                                  <  1:228041/150‑1 (MQ=255)

                                         tCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGtt                                                                                                    >  2:142911/1‑151 (MQ=255)

                                         tCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGtt                                                                                                    >  1:784464/1‑151 (MQ=255)

                                           gACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGttt                                                                                                   >  2:1184327/1‑150 (MQ=255)

                                            aCGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGGTTATCCATTTGCGCCCGGGTTTc                                                                                                  >  2:582644/1‑150 (MQ=255)

                                              gCACAGCCTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGCCACCCCTTGTTGATAATGGCCGATGGTTGCTTTCTAACGTCGGTGTATTTTCTCGTT‑TC‑CCCCCC‑CTCCAGCTGCCACCATTGTTCATCCACTTGCGGCCTGGTTTCAt                                                                                                >  2:253474/1‑150 (MQ=255)

                                                   gCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCCACCATAGTTTATCCATTTGCGCCCGGGATTCATCGTGaa                                                                                          >  2:828107/1‑151 (MQ=255)

                                                   gCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGaa                                                                                          >  1:846991/1‑151 (MQ=255)

                                                    ggtagtaAAAGGGCCCGCCGGGGTCTGGTTCTCGACGCCCATTTTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa                                                                                           <  1:1117153/148‑1 (MQ=255)

                                                     gtagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCCACCATAGTTTATCCCTTTGCGCCCGGGTTTCCTCCTTAAGt                                                                                        >  1:1133240/1‑151 (MQ=255)

                                                     gtagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGt                                                                                        >  2:465892/1‑151 (MQ=255)

                                                      tagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TCCCCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGaaat                                                                                        >  2:541834/1‑149 (MQ=255)

                                                      tagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                                                                       >  1:837517/1‑151 (MQ=255)

                                                      tagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                                                                       >  2:574678/1‑151 (MQ=255)

                                                        gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                                                                      >  1:668346/1‑150 (MQ=255)

                                                        gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                                                                      >  1:462584/1‑150 (MQ=255)

                                                         aaaaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGAGGGTAGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                                                                      <  1:1173239/148‑1 (MQ=255)

                                                          aaaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGa                                                                                   >  2:986719/1‑151 (MQ=255)

                                                                   cGCCAGCTTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGAGGTTTGATTTCTAACGTCGGGGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAg                                                                          <  1:938975/151‑1 (MQ=255)

                                                                   cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAg                                                                          >  1:141155/1‑151 (MQ=255)

                                                                      cggCGTCGGGTTCTCGACGCCCTTTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATg                                                                       <  2:76945/149‑1 (MQ=255)

                                                                         cGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACTTCGGTGATTTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATg                                                                       <  2:28400/148‑1 (MQ=255)

                                                                                ttCTCGACGCCCATTTTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCTTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtg                                                             <  1:1103178/151‑1 (MQ=255)

                                                                                ttCTCGACGCCCATTTTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtg                                                             <  2:402524/151‑1 (MQ=255)

                                                                                         cccATTGTTGCTAATGGCCGATGTTTGATTTCTAACGTCGGTGAATTTTCTTGTT‑TA‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTCTCATCCTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACg                                                     >  2:473674/1‑150 (MQ=255)

                                                                                         cccATTGTTGCTAATGGCCGATGGTTGATTTCTACCGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc                                                    <  1:643927/151‑1 (MQ=255)

                                                                                          ccttttttgCTAATGGCCGATGGTTGATTTCTACCGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCa                                                   <  1:284040/145‑1 (MQ=255)

                                                                                           cttttttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAg                                                  <  2:749684/146‑1 (MQ=255)

                                                                                           cATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGCTGCGCCTGAGTGAGTGTAccccg                                                  >  1:76655/1‑147 (MQ=255)

                                                                                               gttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGa                                              >  2:5572/1‑151 (MQ=255)

                                                                                                  gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATaa                                           >  2:885650/1‑151 (MQ=255)

                                                                                                     aaTGGCCGATGGTGGATTTCTACCGTCGGTGAATTTTCTCTTTCC‑‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCa                                        <  1:1148216/151‑1 (MQ=255)

                                                                                                          ccaaTGTTTGATTTCTACCGTCGGTGAATTTTCTCGTTCC‑‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACAcc                                   <  2:239596/148‑1 (MQ=255)

                                                                                                                gTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGt                              >  2:1037188/1‑150 (MQ=255)

                                                                                                                     tttCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGt                        >  1:631478/1‑151 (MQ=255)

                                                                                                                     tttCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGt                        >  1:1093441/1‑151 (MQ=255)

                                                                                                                      ttCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGttt                                                                                                                       >  1:107913/1‑55 (MQ=255)

                                                                                                                      ttCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGtt                       >  1:274464/1‑151 (MQ=255)

                                                                                                                      ttCAAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGttt                                                                                                                       <  2:107889/55‑1 (MQ=255)

                                                                                                                       tCTAACGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTc                      <  1:23793/151‑1 (MQ=255)

                                                                                                                            cGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTAc                 <  1:464227/151‑1 (MQ=255)

                                                                                                                            cGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTAc                 <  2:401621/151‑1 (MQ=255)

                                                                                                                            cGTCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTAc                 <  1:115453/151‑1 (MQ=255)

                                                                                                                            cGTCGGTAAATTTTCTCGTTCC‑‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTAc                 <  2:996582/151‑1 (MQ=255)

                                                                                                                              tCGGTGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTACAt               >  1:1085911/1‑151 (MQ=255)

                                                                                                                               cgttGAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTACATc              <  2:678507/148‑1 (MQ=255)

                                                                                                                                ggTGAATTTTTCCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTACATc              <  2:660443/150‑1 (MQ=255)

                                                                                                                                   gAATTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTACATCACaa          >  1:296435/1‑151 (MQ=255)

                                                                                                                                     aTTTTCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTACATCACAATg        >  2:1019625/1‑151 (MQ=255)

                                                                                                                                      ttttCTCGTTCC‑‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTACATCACAATGc       <  2:1263171/151‑1 (MQ=255)

                                                                                                                                      ttttCTCGTTCC‑‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTGCATTACATCACAATGc       <  2:566241/151‑1 (MQ=255)

                                                                                                                                      ttttCTCGTTCC‑‑CCCCCC‑CGCCAGCGCCAACCATAGGTTATCCAGTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGGGTACGCAGACGGTAATCAACACCAGCGTCCACGTTCATTACAGCACAATGc       <  2:680481/151‑1 (MQ=255)

                                                                                                                                      ttttCTCGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCGCGTTCATTACATCACAATGc       <  2:529124/151‑1 (MQ=255)

                                                                                                                                         tctcGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTACATCACAATGCTGt    <  1:87239/151‑1 (MQ=255)

                                                                                                                                           tcGTT‑TC‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTACATCACAATGCTGttt  <  1:143458/151‑1 (MQ=255)

AGAGACGTGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTT‑T‑‑CCCCCC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTTCATTACATCACAATGCTGTTT  >  NC_000913/3379115‑3379403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`