Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	59.5%	45.0 / 100.9	74	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (5/25);  new base G (44/0);  total (49/25)
Fisher's exact test for biased strand distribution p-value = 4.06e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-10

GCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGG  >  NC_000913/354144‑354434                                                                                                                                                 |                                                                                                                                                     gCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAg                                                                                                                                                 <  2:657147/150‑1 (MQ=255)  cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGa                                                                                                                                                <  1:344101/150‑1 (MQ=255)  cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGa                                                                                                                                                <  1:124184/150‑1 (MQ=255)    tCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGgggtggggtgggagagg                                                                                                                                              >  2:741388/1‑143 (MQ=255)    tCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAATgg                                                                                                                                             >  2:1086348/1‑150 (MQ=255)             cTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAgg                                                                                                                                   <  1:679833/151‑1 (MQ=255)                 cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGGT‑GGAAggggcg                                                                                                                               >  2:395953/1‑148 (MQ=255)                 cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCGCTGGAAAgggag                                                                                                                                >  1:1015161/1‑149 (MQ=255)                   gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAAAGGGCG‑GAAAGGGCAggg                                                                                                                             >  1:421449/1‑150 (MQ=255)                   gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGGGCG‑GGAAGGGGAggg                                                                                                                             >  1:906736/1‑150 (MQ=255)                     tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtgg                                                                                                                           <  2:933819/151‑1 (MQ=255)                         aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGGGGT‑GGAAGGGGAggtgggggg                                                                                                                       >  2:308798/1‑150 (MQ=255)                          cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCT‑GGAAGGGGCggtggggggg                                                                                                                      >  2:1582117/1‑149 (MQ=255)                          cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAGAAGCGCT‑GGAAGGGGAgggggcgggg                                                                                                                      >  2:1516370/1‑149 (MQ=255)                              aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCT‑GAAAGGGCAgggggggggggggg                                                                                                                  >  1:481796/1‑148 (MQ=255)                              aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCT‑GAAAGGGGAgggggcgggggggg                                                                                                                  >  1:1348243/1‑148 (MQ=255)                                 ctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGa                                                                                                                                                >  1:527090/1‑119 (MQ=255)                                 ctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGGATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGa                                                                                                                                                <  2:526897/119‑1 (MQ=255)                                   cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTc                                                                                                             <  1:1526366/151‑1 (MQ=255)                                     ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCT‑GAAAGGGCAGGGGGGGGGGGGGGTTGTCAt                                                                                                           >  1:1470625/1‑151 (MQ=255)                                        gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAAATGCGCT‑GAAAGGGGAGGTGGCGGGGGCGGTTGTCATTc                                                                                                         >  2:1542146/1‑150 (MQ=255)                                         cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAGATGCGGTGGAAAGGGCGGGGGGGGGGGGGGttgtgatttcg                                                                                                        >  2:928231/1‑142 (MQ=255)                                             gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCCTCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGGGCT‑GACAGGGGCGGTGGCGGGGGGGTTTTTTCTTCCGgaaa                                                                                                   >  2:888046/1‑150 (MQ=255)                                             gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAATGCGCT‑GAAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGgaaa                                                                                                   >  2:224557/1‑150 (MQ=255)                                             gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAATGCGCT‑GAAAGGGGAGGGGGGGGGGGGGGTTTTCATTTCGAAag                                                                                                   >  1:1114771/1‑151 (MQ=255)                                              aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAag                                                                                                   <  1:832980/150‑1 (MQ=255)                                              aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCACTCCGAAag                                                                                                   <  1:259470/150‑1 (MQ=255)                                              aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAGATGCGCT‑GGAAGGGGCGGGGGCGGGGGGGGTTGTCCTTCCGAAagg                                                                                                  >  2:216517/1‑150 (MQ=255)                                                 aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGGGGGGGCGTTTGTTCTTTCGAAagagag                                                                                               >  2:1371500/1‑151 (MQ=255)                                                 aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCAATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAagagag                                                                                               <  2:1269238/151‑1 (MQ=255)                                                  tGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGCTGGAAAGGGC‑GGGGGGGGTGGGGGTTGTCATTCCGGAagagag                                                                                               >  1:1436010/1‑150 (MQ=255)                                                   gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGAAGCGCT‑GGAAGGGGCGGTGGGGGGGGGGGTTGTTATTCCGGAAGAGAGC‑g                                                                                            >  2:1555386/1‑151 (MQ=255)                                                   gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGGGGGGGGGGGGGTTGTCTTTCCGAAagaggggg                                                                                             >  1:1409375/1‑147 (MQ=255)                                                   gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCC‑GGAAAGGGAGGGGGGGGTGGGGGTTGTCATTCCGGAagagaggg                                                                                             >  2:942246/1‑149 (MQ=255)                                                   gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAGATGCGCT‑GGAAAGGCAGGGGGGGGGGGGGGTTGTCCTTCCGGAagagaggg                                                                                             >  1:724118/1‑149 (MQ=255)                                                   gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAATGCGCC‑GGAAGGGGAGGGGGCGGGGGCGGTTGTCATTTCGAAAGAGAGC‑g                                                                                            >  2:1343700/1‑151 (MQ=255)                                                   gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGGGGCGGGGGGTGGGGGGAAAGAAGCGCT‑GAAAGGGCAGGGGGGGGGGGGGGTTGTTCTTCCGAAAGAGAGC‑g                                                                                            >  1:602727/1‑151 (MQ=255)                                                     aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GAc                                                                                          <  1:718047/151‑1 (MQ=255)                                                     aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGGC‑GGAAGGGGGGGGGGGGGGGGGGGTTGGCATTCCCGAagagagggga                                                                                           >  2:777250/1‑147 (MQ=255)                                                       ttAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAg                                                                                        <  1:167322/151‑1 (MQ=255)                                                          aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCt                                                                                     <  1:1584156/151‑1 (MQ=255)                                                             ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTgg                                                                                   <  1:1412996/150‑1 (MQ=255)                                                                ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAg                                                                                <  1:1061476/150‑1 (MQ=255)                                                                ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAg                                                                                <  2:1475234/150‑1 (MQ=255)                                                                ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAg                                                                                <  2:619296/150‑1 (MQ=255)                                                                   ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGGGGCGGGGGGTGGGGGGGAAGATGGGCC‑GGAAGGGGAGGGGGGGGGGGGGGTTGGCATTCCGAAAGAGAGG‑GGCAGGCTGGGAGATCg                                                                            >  1:571000/1‑151 (MQ=255)                                                                       tCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGAt                                                                        <  1:83872/151‑1 (MQ=255)                                                                         ggCTGGGGACGCGCGAGTTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGGGGGGGGGGGGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGGGAtgt                                                                      >  1:221181/1‑151 (MQ=255)                                                                         ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCGCT‑GGAAAGGCAGGGGGGGGGGGCGGTTGTCCTTCCCTAAGAGAGCGGACA‑TCTGGGAGAGCGCGAtgt                                                                      >  2:620289/1‑151 (MQ=255)                                                                          gCTGGGGGCGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGAtgt                                                                      <  2:281370/150‑1 (MQ=255)                                                                          gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAAAGCCCT‑GAAAGGGGCGGTGGCGGCGGCGTTTGTCATTTCCAAAGAGAGG‑GGCAGTCTGGAAGATCGGGAtgtg                                                                     >  1:453235/1‑151 (MQ=255)                                                                          gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGGGCT‑GAAAAGGCAGGGGGGGGGGGCGGTTGTCATTCCGAAAAAGAGG‑GAAAGGCTGGAAGATCGGGagggg                                                                     >  1:351404/1‑147 (MQ=255)                                                                                        agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAg                                                                                <  1:732659/126‑1 (MQ=255)                                                                                        agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGGGCT‑GAAAGGGGAGGGGGGGGGGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGGGATGTGGCGCCCTCGCAaga                                                       >  1:1245472/1‑151 (MQ=255)                                                                                          attggtgagggTATTTCCGGGCATCCGGGCGTTGCCGAAGTGGCGTTGGTTGGGGTGAAAGATGCGCT‑GAATGGGCAGGTGGCGGTGGCGTTTATCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCTCACTCGCAagata                                                     <  2:306943/145‑3 (MQ=255)                                                                                          aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGAAGGGCT‑GAAAGGGGAGGGGGGGGGGGGGTTTGTTATTTCGAAAGAGAGG‑GACAGTCTGGAAGATCGTGGTGTGGGGCCCTCGCAAggga                                                     >  1:467863/1‑151 (MQ=255)                                                                                          aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GGAAGGGGAGGGGGCGGGGGGGGTTGTCATTCCGAAAAAGAGC‑GACAGTCTGGGAGATCGTGATGTGGGGCACCCGCAAgaga                                                     >  2:1502212/1‑151 (MQ=255)                                                                                          aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAATGGGCT‑GAAAGGGGAGGGGGGGGGGGCGTTTGTCCTTCCGAAAGAAAGC‑GGCAGGCTGGGAGAACGTGATGTGGCGCACCCCCAAgaga                                                     >  1:1565347/1‑151 (MQ=255)                                                                                            tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAg                                                   <  2:18908/151‑1 (MQ=255)                                                                                                  gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCT‑GAAAGGGCAGGGGGGGGGGGCGGTTGTCATTTCGAAAAAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                             >  2:505123/1‑151 (MQ=255)                                                                                                  gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAAAGCGCT‑GGAAGGGGCGGGGGGGGGGGGGGTTGTTATTCCGAAAGAGAGG‑GGAAGTCTGGAAGACCGGGATGGGGCGCACCCCCAAGAGAAGGCGAtt                                             >  2:814526/1‑151 (MQ=255)                                                                                                  gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGGGCACTCGCAAGAGAAGGCGGtt                                             >  1:845947/1‑151 (MQ=255)                                                                                                  gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGGGGGGGGGTTTGTCATTCCGAAAGAGAGG‑GACAGTCTGGAAGATCGGGGTGTGGGGCACTCGCAAGAGAAGGGGAtt                                             >  1:426868/1‑151 (MQ=255)                                                                                                    gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGGTGGGGGGCACTCGCAAGAGAAGGGGATTAt                                           >  1:565640/1‑151 (MQ=255)                                                                                                     tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTAt                                           <  1:208176/150‑1 (MQ=255)                                                                                                      aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATgg                                         <  1:1340549/151‑1 (MQ=255)                                                                                                      aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGAAGCGCT‑GAAAGGGCAGGGGGGGGGGGCGGTTTTCCTTCCGAAAGAGAGG‑GGCAGTTTGGGAGATCGTGATGTGGGGCACTCGCAAGAGAAGGGGGTTATgg                                         >  1:1568195/1‑151 (MQ=255)                                                                                                      aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GGAAGGGCAGGGGGGGGGGGCGGTTTTCATTCCGGAAGAGAGC‑GACAGTCTGGAAGAACGTGGTGTGGCGCACTCGCAAGAGAAGGCGATTATgg                                         >  2:577455/1‑151 (MQ=255)                                                                                                             gCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGG‑GACAGGCTGGGAGATCGGGATGTGGCGCCCCCGCCAGAGAAGGCGATTATGGCGCtggg                                  >  1:1037263/1‑150 (MQ=255)                                                                                                               cATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGGGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGGGATGTGGCGCACCCGCCAGAGAAGGGGGTTTTGGCGCtggggg                                >  1:1159083/1‑151 (MQ=255)                                                                                                                                 gTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCaa                >  2:1464913/1‑149 (MQ=255)                                                                                                                                       gtggtTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgc         <  1:593425/150‑1 (MQ=255)                                                                                                                                         ggtTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg       >  1:1380493/1‑150 (MQ=255)                                                                                                                                             ggggTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg  >  1:207812/1‑151 (MQ=255)                                                                                                                                              gggTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGGGGACAGCCAGATTGGCAACTTTGGCCGCCCgg  >  1:53344/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                     GCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGC‑GACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGG  >  NC_000913/354144‑354434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑