Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	357,890	0	C	A	59.7%	‑5.4 / 58.3	62	L267F (TTG→TTT)	cynR	transcriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (17/8);  new base A (0/37);  total (17/45)
Fisher's exact test for biased strand distribution p-value = 1.46e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.13e-05

GATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGT‑CAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATG  >  NC_000913/357742‑358037                                                                                                                                                               |                                                                                                                                                     gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGT‑CAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGc‑aacaa                                                                                                                                                    >  1:569531/1‑151 (MQ=255) gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGT‑CAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGc‑aacaa                                                                                                                                                    >  2:1553464/1‑151 (MQ=255) gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGT‑CAGCGGCTACCGAGATTCATTTCCGCCAACAACCGCGAATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCCGCTATTTTT‑CCGC‑CGc‑aacaa                                                                                                                                                    >  2:578935/1‑151 (MQ=255)     aGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGT‑CAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGC‑AACAAAAc                                                                                                                                                 >  1:415924/1‑150 (MQ=255)      gCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGT‑CAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGC‑AACAAAAccg                                                                                                                                               >  1:775338/1‑151 (MQ=255)                   ggCAATTCCAGCCGCAGACCTGTGT‑CAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGC‑AACAAAACCGCCGTTCTCTCCag                                                                                                                                  >  1:505602/1‑151 (MQ=255)                   ggCAATTCCAGCCGCAGACCTGTGT‑CAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGC‑AACAAAACCGCCGTTCTCTCCag                                                                                                                                  >  1:195687/1‑151 (MQ=255)                      attcccggCCGAAGCCCGGTGC‑AAGCGCCTCCCTTAATTCTTTCCCCCCAACACCCGCTCTTTTCCCC‑AA‑CCCCATTTG‑AAAAATG‑CCCT‑CGGCGGGGG‑CTGTCGGCCATTTTTTTTC‑CCGC‑CCC‑AACAAAACCGCCGTTCTCTCCagtag                                                                                                                               <  1:1327964/144‑1 (MQ=255)                      attccccgCCGAAGACCTGTGT‑CGGGGCCTCCCGTGATTCATTCCCCCCAACACCCGCGCTTTTACCC‑AA‑CCCATTGTGAAAAAAGG‑CCTC‑C‑GGGGGGG‑TTTTCTGCCAGCATTTTTC‑CCGC‑CCA‑AACAAAACCGCCGTTCTCTCCagtag                                                                                                                               <  1:958434/144‑1 (MQ=255)                      atccccACCCGCAGCCGTGTGC‑CGGCGCCTACGGTGTTTCATTCCCCCCAACACCCGCGATTTTACCC‑AC‑CCCATTGTCCAAAAATC‑CCCT‑C‑GGGGCGG‑CTGTCTGCAGGTTTTTTTC‑CCGC‑CGC‑AAAAAAACCGCCGTTCTCTCCagtag                                                                                                                               <  2:371554/147‑1 (MQ=255)                      actcccggCCGCAGCCTTGTGC‑CGGCGGCTACGGTGATTCATCCCCCCCAACCCCCGCGCTTTCCCCC‑AA‑CGCCATGTGAAAAAAGG‑CCTC‑C‑GCGGCGG‑CTGTCGGCAGGTTTTTTTC‑CCGC‑CGA‑AAAAAAACCGCCGTTCTCTCCagtag                                                                                                                               <  2:44042/144‑1 (MQ=255)                      aaccccAGCCGAACCCTTGTGA‑CGGCGGCCACTGTGATACTTCCCCGCAAACAACCGCTCTTTTACCA‑AA‑CGCATTGTAAAAAAATC‑CCTC‑C‑GCGGCGG‑CTGCCTGCCGGTTTTTTTC‑CCGC‑CGA‑AAAAAAACCGCCGTTCTCTCCagtag                                                                                                                               <  2:1090162/147‑1 (MQ=255)                      aaTCCCAGCCGCAGA‑CTGGGTCCAGCGCCACCCGGAATTCATTCCCGCAAACACCCGCGCTTTTCCCA‑AC‑CGCCTGGTGCAAAAATC‑CCTC‑C‑GCGGGGG‑CTTTCGGCAGGTTTTTTTC‑CCGC‑CGA‑AAAAAACCCGCCGTTCTCTCCagtag                                                                                                                               <  2:368585/151‑1 (MQ=255)                       aTTCCAGCCGCAGACCTGTGT‑CAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGC‑AACAAAACCGCCGTTCTCTCCagtagt                                                                                                                              >  1:740216/1‑151 (MQ=255)                       aTTCCAGCCGCAGACCTGTGT‑CAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGC‑AACAAAACCGCCGTTCTCTCCagtagt                                                                                                                              >  1:1623062/1‑151 (MQ=255)                              ccGCAGACCTGTGT‑CAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg                                                                                                                       >  2:281465/1‑151 (MQ=255)                                   gACCTGTGT‑CAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCgggggcagg                                                                                                                  >  1:1106021/1‑146 (MQ=255)                                   gACCTGTGT‑CAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGcgaca                                                                                                                  >  2:402903/1‑147 (MQ=255)                                    aCCTGTGT‑CAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCgggggtcgag                                                                                                                 >  2:802305/1‑145 (MQ=255)                                     cttgtgT‑CGGCGGATCCCGTGATTCTTTCCCCCCAACACCCGCCCTTTTACCC‑AC‑CGCCAGGTGAAAAAATG‑CCTT‑C‑GGGGGGG‑CTTTCTGCAAGTTTTTTTC‑CCCC‑CGA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAgag‑a                                                                                                               <  1:509576/149‑1 (MQ=255)                                     ccTGTGT‑CAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCgggggcaggga                                                                                                                >  1:21410/1‑144 (MQ=255)                                        gtgt‑CAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCggggggcgaggaaa                                                                                                             >  2:355973/1‑141 (MQ=255)                                        gtgt‑CAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCgggggcaggggaaa                                                                                                             >  1:452466/1‑141 (MQ=18)                                            ccggCGTCTACGGTATTTCATTCCCCCCAACACCCGGCCATTACCCC‑AC‑CGCGATG‑GCAAAAAAG‑GCTT‑G‑GGGGGGGTGGGTCGACCATCTGTTTTC‑CCCC‑CGC‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAg                                                                                                          <  1:337003/147‑1 (MQ=255)                                                    tcccgtttttCTTTCCCCCCAACACCCGCG‑ATTTTTCC‑AA‑CCGCAAGGTAAAAAATG‑GCTTCC‑GGGGGGG‑CTGCCTCCCAGTTTTTTTC‑CCGC‑GGC‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGcc                                                                                                 <  1:411282/143‑1 (MQ=255)                                                    tACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAG‑GAAAAACTTTaaaa                                                                                                  >  1:1233587/1‑148 (MQ=25)                                                    ccctgtgtTTCATTCCCCCAAACACCCG‑GCTTTTTACC‑AACCCCCGTGTGAAAAAATC‑CTTC‑C‑GGGGGGG‑CGGCCTCCCGGTTTTTTTC‑CCGC‑CAA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGcc                                                                                                 <  2:980553/147‑1 (MQ=255)                                                    cccggtatttCATCCCCGCAAACACCCGCGTTTTTCCCC‑AC‑CCCCTGGAGAAAAATTC‑CCTC‑C‑GCGGCGG‑TTGTCGCCCGGTTTTTTTC‑CCGC‑CAA‑AAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGcc                                                                                                 <  2:602424/143‑1 (MQ=255)                                                     aCCGTGATTCATTTCCGCCAACAACCGCGCATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAa‑aaaaaacattttacc                                                                                                 >  1:1366820/1‑138 (MQ=25)                                                           aTTCATTTCCGCCAACAACCGCGCATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGTAAGAG‑AAACAACTTTaacaccccgcgt                                                                                          >  2:1404033/1‑141 (MQ=25)                                                            ttcttcccccacaacaaCCGC‑TATTTAACCAAA‑CCCCGTGGGCAAAACTG‑CGTG‑G‑GGGGGGG‑CTGTCGGCAGGTTTTTTTC‑CCGC‑CAA‑AAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCAtgt                                                                                         <  2:863313/140‑1 (MQ=255)                                                             tctttccccccaccaCCCGCCCATTTACCC‑AC‑CCCCTTGTGCAAAAATC‑CTTC‑C‑GCGGCGG‑CTGTCCCCCAGCTTTTTTC‑CCGC‑CCC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCAtgtt                                                                                        <  1:763080/142‑1 (MQ=255)                                                              ttttccccccAACCCCCGCGCTTTTCCCC‑AA‑CCCCATGTAAAAAAATG‑CCTT‑C‑GGGGGGG‑CTGTCTGCCAGTTTTTTTC‑CCCC‑CGC‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCAtgttg                                                                                       <  1:347240/143‑1 (MQ=255)                                                              ctttccccccaccaCCCGCGCTTTTACCA‑AC‑CGCCATGTGAAAAAATC‑CCTC‑C‑GCGGCGG‑CTGTCTGCGAGTTATTTTC‑CCGC‑CGC‑AAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCAtgttg                                                                                       <  2:191470/143‑1 (MQ=255)                                                              ctttccccccAACACCCGCGCTTTTCCCC‑AA‑CGCCATGTAAAAAAATG‑CCTT‑C‑GCGGGGG‑CTTCCTCCCAGTTTTTTTC‑CCGC‑CGC‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCAtgttg                                                                                       <  1:199571/143‑1 (MQ=255)                                                              catcccccccAACACCCGCGCTTTTACCC‑AC‑CGCCAGGTAAAAAAATC‑CCTG‑C‑GGGGCGG‑CTGTCTCCCAGTTTTTTTC‑CCGC‑CGA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCAtgttg                                                                                       <  2:78311/143‑1 (MQ=255)                                                                   ccaccAACACCCGCGCTTTCACCC‑AA‑CCCCATGTAAAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTC‑CCGC‑CAC‑AAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTgg                                                                                  <  2:1529981/148‑1 (MQ=255)                                                                    ccccaccaCCCGCGCTTTCCCCC‑AC‑CCCCTTGTGAAAAAATG‑CCTT‑C‑GGGGCGG‑CTGTCTGCCAGCTTTTTTC‑CCGC‑CAC‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGc                                                                                 <  2:499741/149‑1 (MQ=255)                                                                                    tttACCC‑AC‑CGCTATGAAAAAAATGG‑CTTT‑C‑GGGGCGG‑CTGTCGGCCTGCTTTTTTC‑CCGC‑CGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAc                                                                 <  1:176602/151‑1 (MQ=255)                                                                                    tttACCC‑AA‑CCCCTTGT‑AAAAAAAG‑CCTTCC‑GGGGCGC‑CTGTCTGCCAGTTTTTTTC‑CCGC‑CAC‑AACAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAc                                                                 <  2:597374/151‑1 (MQ=255)                                                                                     tttacc‑AA‑CCCCATTGGAAAAAAAG‑CCTTGC‑GCGGCGG‑TTGGATGCCAG‑TTTTTTC‑CCGCGCCA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAc                                                                 <  2:1336393/147‑1 (MQ=255)                                                                                     ttATCC‑AA‑CCCCCTGGGAAAAAAGGCCCTT‑C‑GCGGGGC‑CTGTCGCCGAGTTTTTTTC‑CCCC‑CGC‑AAAAAAACCGCCGTTCTCTCCAGTAATGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAc                                                                 <  1:1366406/151‑1 (MQ=255)                                                                                        cccccaacccatggtgCAAAACTC‑CTTC‑C‑GCGGCGC‑CTTTCTGCTAGTTTTTTTC‑CCGC‑CAA‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACaa                                                               <  2:727415/138‑1 (MQ=255)                                                                                          cccaccccctgggaaaaaaaGC‑CCTC‑C‑GGGGCGG‑TTTTC‑GCCCGCTTTTTTTCCCGC‑CGA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAtg                                                             <  1:706090/136‑1 (MQ=255)                                                                                                   ttgtcaaaaaaCC‑CCTC‑C‑GCGGCGG‑CTTTCTGCCATTTTTTTTC‑CCGC‑CGA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAggg                                                    <  2:1206674/145‑1 (MQ=255)                                                                                                    tgtcaaaaagTG‑CTTC‑C‑GGGGGGG‑TTTTCGGCCAGTTTTTTTC‑CCGC‑CCA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGa                                                   <  1:1598891/146‑1 (MQ=255)                                                                                                    tgcgaaaaagTC‑CCTC‑C‑GCGGCGG‑CTGTCTGCCAGCTTTTTTC‑CCGC‑CGA‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGa                                                   <  2:166928/146‑1 (MQ=255)                                                                                                    tgaacAAAAGTC‑CCCT‑C‑GCGGCGG‑CTGTCTGCCAGTTTTTTTC‑CCGC‑CGC‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGa                                                   <  2:109731/147‑1 (MQ=255)                                                                                                     gtgCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAGAAAAAAACTTTAACCCCG‑CCTGTTTTGTGGGCGACGCCGCCGGTTAAAAAGTGGGAAGGGag                                                  >  2:1399586/1‑150 (MQ=255)                                                                                                         aaaaaTC‑CCTT‑C‑GGGGGGG‑TTTTCGCCCAGTTTTTTTC‑CCGC‑CCA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgc                                              <  1:677849/151‑1 (MQ=255)                                                                                                                                ttgtctccaatttATTTTC‑CCGC‑CGA‑AAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc                           <  1:357415/139‑1 (MQ=255)                                                                                                                                ttgcctgcagtttATTTTC‑CCCC‑CAA‑AAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc                           <  2:441985/139‑1 (MQ=255)                                                                                                                                ttGTCTCCAAGTTATTTTC‑CCGC‑CGA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc                           <  1:1270052/150‑1 (MQ=255)                                                                                                                                ctttctgccgttttttttc‑CCGC‑CGA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc                           <  2:769005/137‑1 (MQ=255)                                                                                                                                ctttctgccgttttttttc‑CCGC‑CGA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc                           <  1:340963/137‑1 (MQ=255)                                                                                                                                ctgcctgcGAGTTTTTTTC‑CCGC‑CGC‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc                           <  2:1403422/151‑1 (MQ=255)                                                                                                                                cTGTCTGCAGGTTTTTTTC‑CCGC‑CGA‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc                           <  2:1105498/151‑1 (MQ=255)                                                                                                                                cTGTCTCCCATTTTTTTTC‑CCCC‑CGA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCGAGAAc                           <  1:1053335/151‑1 (MQ=255)                                                                                                                                cTGTCGGCCAGTTTTTTTC‑CCGC‑CGA‑AAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAc                           <  1:844665/151‑1 (MQ=255)                                                                                                                                      cccgttttttttc‑CCGC‑CGA‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCgcgc                      <  1:1097336/142‑1 (MQ=255)                                                                                                                                           tttttttc‑CCCC‑CCC‑AAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTg                <  1:1379978/148‑1 (MQ=255)                                                                                                                                           cTATTTTC‑CCGC‑CGA‑AAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTg                <  2:1077036/151‑1 (MQ=255)                                                                                                                                             tttttc‑CCCC‑CCC‑AAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAg              <  1:403014/150‑1 (MQ=255)                                                                                                                                              ttttc‑CCGC‑CCCAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAg              <  2:676442/151‑1 (MQ=255)                                                                                                                                                    cccc‑cga‑AAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGGAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGcc        <  1:1454430/148‑1 (MQ=255)                                                                                                                                                            aaacaaACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATg  <  2:1096190/150‑1 (MQ=255)                                                                                                                                                               |                                                                                                                                                     GATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGT‑CAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCC‑AA‑CGCCATGTGCAAAAATG‑CCTT‑C‑GCGGCGG‑CTGTCTGCCAGCTATTTTT‑CCGC‑CGC‑AACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATG  >  NC_000913/357742‑358037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑