Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	44.9%	133.4 / 33.0	89	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (12/37);  new base G (40/0);  total (52/37)
Fisher's exact test for biased strand distribution p-value = 1.10e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCG  >  NC_000913/354138‑354436                                                                                                                                                       |                                                                                                                                                      aCTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGt                                                                                                                                                       <  1:308508/151‑1 (MQ=255)      ggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGaaa                                                                                                                                                   <  1:29147/150‑1 (MQ=255)        cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGGATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGa                                                                                                                                                 <  2:1079569/150‑1 (MQ=255)        cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGa                                                                                                                                                 <  2:910416/150‑1 (MQ=255)        cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGa                                                                                                                                                 <  1:1127175/150‑1 (MQ=255)        cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGa                                                                                                                                                 <  2:552795/150‑1 (MQ=255)        cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTCGGGGTGAAAGa                                                                                                                                                 <  2:1418793/150‑1 (MQ=255)          tCCGTGACGCTGACGGTTATCACTTTATTATCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGATGCCGAAGCGGCGGGGGGCGGGGGGGAAggaga                                                                                                                                              >  2:539682/1‑147 (MQ=255)                aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGCGGCGGTGGGTGGGGGGGAAAATggg‑ccgca                                                                                                                                       >  2:1087466/1‑147 (MQ=255)                 cGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGaaa                                                                                                                                      <  2:1247990/151‑1 (MQ=255)                 cGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGGAAGATGCG‑CTGcaa                                                                                                                                      >  1:1183602/1‑151 (MQ=255)                   cTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAgg                                                                                                                                    <  2:1325514/151‑1 (MQ=255)                       cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCG‑CTGAAAGGGCAg                                                                                                                                >  1:1282812/1‑151 (MQ=255)                       cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCG‑CTGAAAAGGGAg                                                                                                                                >  2:663425/1‑151 (MQ=255)                            aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAggtggc                                                                                                                           <  2:1465047/151‑1 (MQ=255)                               aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAggtggcggt                                                                                                                        <  1:1461745/151‑1 (MQ=255)                               aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAggtggcggt                                                                                                                        <  1:1138592/151‑1 (MQ=255)                                cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCCAAGTGGCGGTGGGTTGGGGGCAAGCGGCG‑TTGTAATGGCAgggggcggtg                                                                                                                       >  1:1432175/1‑151 (MQ=255)                                    aTTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAGATGCG‑CCGGAAGGGCAGGTGGGGGTGGCGt                                                                                                                   >  1:750322/1‑151 (MQ=255)                                        tcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCGGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGt                                                                                                               <  1:341669/151‑1 (MQ=255)                                        tcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGt                                                                                                               <  1:810783/151‑1 (MQ=255)                                                 cTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGGG‑CCGGAAGGGGAGGGGGGGGGGGGGTTTTTCATTCCGaa                                                                                                      >  2:1455559/1‑151 (MQ=255)                                                   gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAAAGCG‑CCGTAAGGGCGGGGGGGGGGGGCGGTTGTCACTCCGtaaa                                                                                                    >  1:346271/1‑150 (MQ=255)                                                    aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAag                                                                                                    <  2:410610/150‑1 (MQ=255)                                                    aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAag                                                                                                    <  2:1213655/150‑1 (MQ=255)                                                       aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAagagag                                                                                                <  1:742121/151‑1 (MQ=255)                                                        tGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGGGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGc                                                                                               <  1:348192/151‑1 (MQ=255)                                                         gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCG‑CTGGAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCg                                                                                              >  1:1120440/1‑151 (MQ=255)                                                         gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAAAGGG‑CGGGAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCCGAagagaggg                                                                                              >  1:1360330/1‑149 (MQ=255)                                                         gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAGATGCG‑CTGAAAAGGGAGGGGGGGGGGGGGGTTTTCCTTTCGAAagagaggg                                                                                              >  2:658109/1‑149 (MQ=255)                                                             ttAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAg                                                                                          <  2:1138126/151‑1 (MQ=255)                                                                     gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCG‑CTGAAAGGGGCGGGGGCGGGGGGGTGTGTTATTGCGAAAGAGAGAGACAggtggggag                                                                                  >  1:48483/1‑143 (MQ=255)                                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                  <  1:1078033/150‑1 (MQ=255)                                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                  <  2:874666/150‑1 (MQ=255)                                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                  <  2:750766/150‑1 (MQ=255)                                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                  <  2:214950/150‑1 (MQ=255)                                                                       cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                                                                                <  1:264008/151‑1 (MQ=255)                                                                          gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAATGCG‑CTGGAAGGGGAGGTGGCGGTGGGGGTTGTTATTCCCAAAAAGAACGACAGTCTGGAAggatgg                                                                             >  1:1116371/1‑146 (MQ=255)                                                                              cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtg                                                                         <  1:664820/151‑1 (MQ=255)                                                                               ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCG‑CTGAAAGGGCAGGTGGGGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGTCTgggaaaacggggtgg                                                                        >  2:998420/1‑138 (MQ=255)                                                                               ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAATGCC‑CTGAAAGGGCCGGGGGGGGGGGGGGTTGGCCTTCCGAAAGAGAGCGACAGGCTGGAAGATCGGGAtgg                                                                        >  2:1464767/1‑150 (MQ=255)                                                                                     ggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcgc                                                                   <  2:1265026/150‑1 (MQ=255)                                                                                     ggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGTTGGGGGGGAAAAAGCG‑CTGAAAGGGCAGGGGGCGGTGGCGGTTGTCATTTCGAGAGAGAGCGAAAGACCGGAAGAttcggaggtggggc                                                                   >  1:1123177/1‑137 (MQ=255)                                                                                              agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCG‑CTGGAAGGGGGGGGGGGGGGGGGGTTTTTTATTTCGGAAAGGAGCGAAAGGCTGGGAAAGCGGGGTGTGGCGCCCTCGCAagg                                                         >  2:1368599/1‑150 (MQ=255)                                                                                                aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga                                                       <  1:481438/151‑1 (MQ=255)                                                                                                aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga                                                       <  2:1184160/151‑1 (MQ=255)                                                                                                aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga                                                       <  1:663080/151‑1 (MQ=255)                                                                                                aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCACTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                        <  1:1388019/150‑1 (MQ=255)                                                                                                aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCG‑CCGAAAGGGCAGGGGGGGGGGGGGTTTGTCATTTCGAAAGAGAGCGACAGTCTGGGAGATCGGGGTGGGGCGGCCCCGCAAaaga                                                       >  2:273625/1‑151 (MQ=255)                                                                                                aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGAAAGATGCGCCTGAAAGGG‑AGGGGGCGGGGGCGGTTGTCCTTCCGAAAAAGGGGGACACTCTTGCAGATGGTGATGTGGCGCCCTCGcccggga                                                       >  1:536719/1‑145 (MQ=255)                                                                                                aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCG‑CTGGAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGAAAAAGAGCGAACGGCTGGAAGATCGTGGTGTGGGGGACTCGCAAgaga                                                       >  1:272272/1‑151 (MQ=255)                                                                                                aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCG‑CTGAAAGGGCAGGGGGGGGGGGGGGTTGTGATTCCGAAAGAGAGGGACAGGCTGGAAGAAGGGGGTGGGGGGCACTCGCAAgaga                                                       >  2:647299/1‑151 (MQ=255)                                                                                                   gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCG‑CTGGAAGGGGAGGTGGGGGGGGCGTTTTTCATTCCGCAAGAGAGCGACCGGCCGGAAGATCGGGGTGTGGCGCACTCGCAAGAGAAgg                                                    >  1:494366/1‑151 (MQ=255)                                                                                                    aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAgg                                                    <  1:681026/150‑1 (MQ=255)                                                                                                        gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGGAAGATGCG‑CTGAAAGGGCAGGGGGGGGGGGGGGTTTTCCTTCCGAAAGAGAGCGGCAGGCTGGGAGATCGGGGTGTGGCGCACTCGCAAGAGAAgggggtt                                               >  2:1430591/1‑146 (MQ=255)                                                                                                        gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCG‑CTGAAAGGGCCGGGGGGGGGGGCGGTTGTTATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGGTGGGGCGCACTCGCAAGAGAAGGCGAtt                                               >  2:1372968/1‑151 (MQ=255)                                                                                                        gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCG‑CTGAAAGGGCAGGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGGTGTGGGGCCCCCGCAAGAGAAGGGGAtt                                               >  2:297345/1‑151 (MQ=255)                                                                                                        gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCG‑CTGAAAGGGCAGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGGtt                                               >  2:333552/1‑151 (MQ=255)                                                                                                        gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGGG‑CTGAAAAGGGAGGGGGGGGGGGGGGTTGTCATTCCGAAAAAGAGGGACAGGCTGGAAGAGAGGGGTGGGGGGCAATCGCAAGaggagggggtt                                               >  1:459218/1‑146 (MQ=255)                                                                                                          gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGGAAGATGCG‑CCGGAAGGGGCGGGGGCGGGGGGGGTTGTCCTTCCGGAAGAGAGGGACAGTCTGGGAGATCGGGGTGTGGCGGCCTCCCCAGAGAAGGCGAGTAt                                             >  1:16202/1‑151 (MQ=255)                                                                                                            aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCG‑CTTGAAGGGCAGGGGGGGGGGGGGTTTTTCATTCCGAAAAAGAGCGACAGTCTGGAAGAACGGGGTGGGGCGCACCCGCAAAAGAAGGGGATTATgg                                           >  2:436336/1‑151 (MQ=255)                                                                                                            aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGGGGCGTTTTTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGGGCACTCGCCAGAGAAGGCGGTTATgg                                           >  2:1127927/1‑151 (MQ=255)                                                                                                            aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGGTGGGGGGAAAGATGCG‑CCGGAAGGGCCGGGGGCGGGGGCGGTTGTGATTCCGAAAGAGGGGGAACGTCTGGCAGGTGGTGGTGTGGCCCACCCGCAAGAGAAAGCGCATATgg                                           >  1:116947/1‑151 (MQ=255)                                                                                                             tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCG‑CTGAAAGGGGAGGGGGGGGGGGGGGTTGTCATTTCGAAAAAGAGGGACAGGCTGGGAGAGCGGGGGGTGGCGCACTCGCCAGGGAAGGGGGTTATggg                                          >  1:11055/1‑150 (MQ=255)                                                                                                             tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAAAGGG‑CCGAAAAGGGAGGGGGGGGGGGGGGTTGTCATTCCCAAAAAGAGCGACAGGCTGGAAGATTGGGATGGGGCGCACCCCCGAAAGAAGGGGATTATgg                                           >  2:414852/1‑150 (MQ=255)                                                                                                              ctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCG‑CCGGAAGGGGCGGGGGCGGGGGGGGTTGTCATTCCGAAAGAGAGCGGCAGTCTGGAAGATCGTGGGGTGGGGCACTCGCAAGAGAAGGCGagttttgcg                                         >  1:1384444/1‑143 (MQ=255)                                                                                                               tcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgcgc                                        <  1:1086942/151‑1 (MQ=255)                                                                                                               tcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgcgc                                        <  2:11069/151‑1 (MQ=255)                                                                                                                   gCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCG‑CTGAAAGGGGAGGGGGGGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGCGGTTATGGCGCtggg                                    >  2:123502/1‑150 (MQ=255)                                                                                                                    ccATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcgc                                                                   <  2:138869/119‑1 (MQ=255)                                                                                                                      aTCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCG‑CTGAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGGGACAGTCTGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGGGATTATGGCGCTGGGGGa                                 >  2:293546/1‑151 (MQ=255)                                                                                                                      aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCG‑CTGAAAGGGGAGGGGGGGGGGGCGGTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGAGCGGGATGGGGGGCACTCGCAAGAGAAGGCGATTATGGCGGtgggggg                                 >  1:734960/1‑150 (MQ=255)                                                                                                                      aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCG‑CTGAAAGGGCAGGGGGCGGGGGCGTTTGTTATTCCGAAAGAGAGGGGCAGTCTGGAAGATCGTGATGTGGCGCCCTCGCAAGAGAAGGCGATTTTGGGGGTGGGGGa                                 >  2:180392/1‑151 (MQ=255)                                                                                                                      aTCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAGATGCG‑CTGAAAGGGCAGGGGGGGGGGGGGTTTGTCATTTCGAAAGAGAGCGACAGTCTGGGAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGGGATTATGGCGCtgggggg                                 >  1:677263/1‑150 (MQ=255)                                                                                                                      aTCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAATGCG‑CCGGAAGGGCAGGTGGGGGTGGCGGTTGTCCTTCCGAAAGAAGGCGAACGTCTGGAAGATCGTGGTGTGGCGCACTCGCCAGAGCAGGCGGATATggtggttgcggg                                 >  1:960027/1‑141 (MQ=255)                                                                                                                       tCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGATGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGAc                                <  2:624287/151‑1 (MQ=255)                                                                                                                           ggCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGc                             <  2:558462/150‑1 (MQ=255)                                                                                                                               ttGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAt                        <  2:1226656/151‑1 (MQ=255)                                                                                                                               ttGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCG‑CTGAAAAGGCAGGTGGGGGGGGCGGTTGTCATTCCGAAAGAGAGGGACAGTCTGGAAGATCGGGATGTGGGGCACTCGCAAGAGAAGGGGATTATGGGGCTGGGGGACAGCCAGAt                        >  2:650785/1‑151 (MQ=255)                                                                                                                                              tggtTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg         >  2:906596/1‑151 (MQ=255)                                                                                                                                              tggtTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg         >  1:929644/1‑151 (MQ=255)                                                                                                                                                   ggggTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg    >  2:1096757/1‑151 (MQ=255)                                                                                                                                                    gggTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg    >  2:646034/1‑150 (MQ=255)                                                                                                                                                    gggTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg    >  2:1283155/1‑150 (MQ=255)                                                                                                                                                    gggTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg    >  1:214748/1‑150 (MQ=255)                                                                                                                                                    gggTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg    >  2:1482961/1‑150 (MQ=255)                                                                                                                                                    gggTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg    >  2:985226/1‑150 (MQ=255)                                                                                                                                                     ggTGAAAGATGCG‑CTGAAAGGGCAGgtggcggtggcg                                                                                                                    <  2:605960/37‑1 (MQ=255)                                                                                                                                                     ggTGAAAGATGCG‑CTGAAAGGGCAGgtggcggtggcg                                                                                                                    >  1:605668/1‑37 (MQ=255)                                                                                                                                                     ggTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcg  >  1:498157/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                      ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCG‑CTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCG  >  NC_000913/354138‑354436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑