Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,423,2900AC48.6% 24.4 / 13.2 37intergenic (+96/+133)yhdZ/rrfFputative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
Reads supporting (aligned to +/- strand):  ref base A (6/13);  new base C (16/2);  total (22/15)
Fisher's exact test for biased strand distribution p-value = 6.29e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

TCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTA‑CTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCC  >  NC_000913/3423146‑3423437
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tCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGGGGCCTT‑cccccccccccc                                                                                                                                                  >  2:92004/1‑148 (MQ=255)
tCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGGGGCCCG‑cccccccccccc                                                                                                                                                  >  1:63393/1‑148 (MQ=255)
tCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTG‑cccccccccccc                                                                                                                                                  >  2:557162/1‑148 (MQ=255)
tCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTG‑cccccccccccc                                                                                                                                                  >  2:215684/1‑148 (MQ=255)
tCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTG‑CCCGcccccccc                                                                                                                                                  >  2:403790/1‑148 (MQ=255)
tCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTC‑cccccccccccc                                                                                                                                                  >  2:210034/1‑148 (MQ=255)
  cTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGGGTCCCT‑Ccccccccccccccg                                                                                                                                               >  2:572896/1‑149 (MQ=255)
    aaaTCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTG‑CCCGCCACCCTCCGGGc                                                                                                                                             <  1:134030/151‑1 (MQ=255)
    aaaTCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTT‑CCCCCCCCCCCCCggg                                                                                                                                              >  2:496781/1‑150 (MQ=255)
          gagCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCGCCCCCCCCCCCCCCCGGCCGttt                                                                                                                                        >  1:464048/1‑150 (MQ=37)
            gCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCG‑CCCCCCCCCCCCCCGGCCGGTGttt                                                                                                                                     >  2:758514/1‑151 (MQ=255)
              gTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTT‑CCCCCCCCCCCCCCGGGCGTTGCTTc                                                                                                                                    >  2:180741/1‑150 (MQ=37)
               tACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGGGTCCCG‑Cccccccccccccccggcccttgtttg                                                                                                                                   >  1:779514/1‑136 (MQ=255)
               tACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCG‑CCCCCCCCCCCCCCGGCCGTTGCtttg                                                                                                                                   >  2:477231/1‑148 (MQ=255)
                   aGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCG‑CCCCCCACCCCCCCCGCCGCTGCTTTTC‑AAc                                                                                                                              >  1:756537/1‑150 (MQ=255)
                    gggCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCG‑CCCCCCCCCCCCCCCGCCGTTgtctctcaca                                                                                                                               >  1:86280/1‑140 (MQ=255)
                          ttttATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTT‑CCCCCCCCCCTCCCGGCCCGTGCTTCTC‑AACGTTCAAAt                                                                                                                      >  1:587777/1‑151 (MQ=25)
                           tttATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATc                                                                                                                     <  2:792122/151‑1 (MQ=255)
                           tttATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATc                                                                                                                     <  2:393774/151‑1 (MQ=255)
                            ttATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATcc                                                                                                                    <  2:602174/151‑1 (MQ=255)
                                     gTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCCG‑CCCCCCCCCCCCCCGGCCGGTGCTTTGC‑AACCTTTCAATcccccccccg                                                                                                            >  2:408849/1‑148 (MQ=21)
                                          ccATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGAtt                                                                                                      <  1:27425/151‑1 (MQ=255)
                                          ccATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTG‑CCCCCCCCCCCCCCGGCCGCTGCTTTGC‑CAAGTTTAAAATCGCCCCccgcggggt                                                                                                      >  2:772102/1‑148 (MQ=18)
                                               aaTTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTcc                                                                                                 <  2:647097/151‑1 (MQ=255)
                                                aTTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATTGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTG‑CCCCCCACCCCCCCGGGCGGTTCTTCGC‑AAAGATTAAAACCCCCCCCGGCGGGGTTGTc                                                                                                  >  2:538078/1‑149 (MQ=18)
                                                   gAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTT‑CCCGCCACCCCCCCGGGCGTTTCTTTGC‑AAACTTATAATCCCCTCCCCGCGGGGTTGTCCtt                                                                                               >  2:651382/1‑148 (MQ=255)
                                                     aTGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCC‑CCCCCCACCCCCCCGGGCGCTGTTTTCT‑AAAAATCAAAATCGCTCCccgcgggattttgcttctc                                                                                            >  2:612013/1‑137 (MQ=12)
                                                        ttAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGGCTGGCAGTTTATGGCGGGCGTCCTG‑CCCGCCCCCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTA‑CTCggg                                                                                        <  1:424333/150‑1 (MQ=255)
                                                         tAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTA‑CTCGGgag                                                                                      <  1:496543/151‑1 (MQ=255)
                                                                     cAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTG‑CCCGCCCCCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTA‑CTCGGGAGAGTGTTCAcc                                                                            <  2:634788/149‑1 (MQ=255)
                                                                     cAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTA‑CTCGGGAGAGTGTTCAcc                                                                            <  2:392186/149‑1 (MQ=255)
                                                                                 ccgtcaggATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCTGCCTT‑ACCCCCCCCCCCCCTGCCCTTGCTTTTC‑AATCGTCAAATTCTCTTCCCGCGGTCTTTTTTTA‑CCCCCGAGAGTGTTCTCCGCCCTacaacac                                                                >  1:199758/7‑148 (MQ=0)
                                                                                 ccTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTT‑CCCCCCCCCCCCCCGGCCGGTGCTTCCC‑CACCTTCAAAACCCCCCCCCGCGGGTTTTTCCTA‑CTCCGGAGGGGGTTTACCCacaaaaaaaaaaa                                                              >  2:552307/1‑146 (MQ=9)
                                                                                            gCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCCC‑CCCCCCCCCCCCCCGGCCGGTGCTTTGC‑AAAGGTCAAATCCCCCCCCCGCGGGGTTTTTCTT‑CTCCGGGGGGGGGTCACCCCCaaaaaaaaaaaaaaaaaaaaag                                                   >  2:352581/1‑149 (MQ=9)
                                                                                             ccTTTCGCTTGATTTGATGTCGGGCAGTTTATGGCGGGCGCCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTA‑CTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGc                                                  <  1:403578/151‑1 (MQ=255)
                                                                                                       gatttgatGTCTGGCAGTTTATGGCGGGCGCCCTG‑CCCCCCACCCCCCGGGCCGCTGCTTTGC‑AACGTTCAAAACCCCCCCCCGCGGGTTTTTCCTT‑CTCCGGAGAGGGTTTACCCACAAAAAAAAGAAAAAAAAAAAAGCCCCGGCTTc                                         >  2:87560/1‑150 (MQ=255)
                                                                                                        atttgatGTCTGGCAGTTTATGGCGGGCGTCCTG‑CCCGCCCCCCCCCCGGCCGGTGCTTCGC‑CAACTTTAAATCCCCTACCCGCGGGGTTGTTCTC‑CTCCGGCGGGTGTTCACCCACAAAACAAAGATAAAAAAAAAAGCCCCGttcttcg                                       >  2:148155/1‑145 (MQ=2)
                                                                                                        atttgatGTCTGGCAGTTTATGGCGGGCGTCCTG‑CCCCCCCCCCCCCCGGCCGGTGCTTCTCAAAGGTTCAAACCGCCCCCCGGCGG‑TTTGTCCTACCCCGGGGGGGGGTTCCCGAACAAACAACAAAAAAAACAAAAGACCCCGCCTTc                                         >  1:317514/1‑150 (MQ=2)
                                                                                                        atttgatGTCTGGCAGTTTATGGCGGGCGGCCCG‑CCCCCCCCCCCCCCGGCCCGTGCTTTGC‑AAAGGTTAAATTCCCTCCCCGCGGGTTTTTCCTT‑CTCCGGGGAGGGTTTACCCCCAAACAACAGAAAAAAAAAAAAAGCCAGGCTTcc                                        >  2:523413/1‑150 (MQ=255)
                                                                                                          ttgatGTCTGGCAGTTTATGGCGGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTA‑CTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGAc                                     <  1:792889/151‑1 (MQ=38)
                                                                                                            gatGTCTGGCAGTTTATGGCGGGCGTCCTG‑CCCCCCCCCCCCCCGGCCGGTGCTTCGC‑CACCCTCCAATCCGCCCCCCGCCGGTTTTTCCTA‑CTCCGGGGGGTGGTCACCCACCAACAACAAATAAAAAAAAAAGCCCACTCCTCCggccg                                   >  1:237480/1‑147 (MQ=2)
                                                                                                                        tttATGGCGGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTA‑CTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGt                         <  2:393855/149‑1 (MQ=38)
                                                                                                                              gcgggcgTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTA‑CTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTAttt                  <  1:500565/150‑1 (MQ=38)
                                                                                                                                  gcgTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTA‑CTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATg              <  2:566356/150‑1 (MQ=38)
                                                                                                                                               ccACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTA‑CTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTcc  >  2:655523/1‑150 (MQ=40)
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TCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACGTTCAAATCCGCTCCCGGCGGATTTGTCCTA‑CTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCC  >  NC_000913/3423146‑3423437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑