Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	53.8%	91.5 / 124.4	78	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (8/28);  new base G (42/0);  total (50/28)
Fisher's exact test for biased strand distribution p-value = 2.48e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.78e-14

ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGG  >  NC_000913/354138‑354434                                                                                                                                                        |                                                                                                                                                     aCTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGt                                                                                                                                                      <  2:623017/151‑1 (MQ=255)       gCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAg                                                                                                                                                 <  2:275050/150‑1 (MQ=255)           ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAGATgcg                                                                                                                                            >  2:1201024/1‑151 (MQ=255)                    tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAggg                                                                                                                                  <  1:1148816/151‑1 (MQ=255)                       cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGTGTAAGAATCGCT‑gcacagg                                                                                                                                  >  1:1247811/1‑142 (MQ=255)                       cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGGGCC‑GAAAGGGGAg                                                                                                                               >  1:358603/1‑151 (MQ=255)                       cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGTTGGGGGGGAAGATGGGCT‑GAAAGGGCAg                                                                                                                               >  2:924502/1‑151 (MQ=255)                        ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAAAAGGGCT‑GAAAGGGCggg                                                                                                                              >  2:1476233/1‑151 (MQ=255)                         gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCT‑GAAAGGGGAggg                                                                                                                             >  2:1227959/1‑150 (MQ=255)                           tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTTGGGGGAAAGATGCGCT‑GAAAGGGCCggggg                                                                                                                           >  2:1567580/1‑151 (MQ=255)                           tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAggtgg                                                                                                                           <  2:48433/151‑1 (MQ=255)                              cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGTTTGGGGGGAAGATGCGCT‑GTAAAGGCCgggggggg                                                                                                                        >  2:372041/1‑151 (MQ=255)                               aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGGAAAATGCGCT‑GGAAGGGGAggggggggg                                                                                                                       >  2:953544/1‑150 (MQ=255)                               aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGGGCT‑GGAAGGGGAggggggggg                                                                                                                       >  1:606428/1‑150 (MQ=255)                                cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCT‑GAAAGGGGAgggggcgggg                                                                                                                      >  2:1119738/1‑149 (MQ=255)                                cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGTTGGGGGGGAAGATGGGCT‑GAAAGGGGAgggggggggg                                                                                                                      >  2:546273/1‑149 (MQ=255)                                cTTTATTCCCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGAAAAAAGGGCA‑GAAAGGGccaggggggggg                                                                                                                      >  2:1678951/1‑140 (MQ=255)                                cTTTATTATCGGGCGAACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTACAGAAATCCGGGCGTTGCCGAAGTGGCGGG‑GGGGGGGGGGGAAAACGGGCG‑GAAAggggggggggggggg                                                                                                                      >  1:450083/1‑139 (MQ=255)                                         cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTc                                                                                                            <  1:247180/151‑1 (MQ=255)                                           ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCa                                                                                                           <  1:1541517/150‑1 (MQ=255)                                                    aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAag                                                                                                  <  2:772909/150‑1 (MQ=255)                                                     cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGCGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGATAATGCGCC‑GAAAGGGCGGGCGGTGGCGGC‑GGTTGTCCTTCCGCAagaa                                                                                                >  1:1077998/1‑150 (MQ=255)                                                     cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGTGAAAGATGGGCC‑GAAAGGGCAGGGGGGGGGGGG‑GTTTGTTATTTCGAAagag                                                                                                >  2:1310654/1‑151 (MQ=255)                                                       aTGTAATTAACGTTGCTGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑TGTTGGGGGGAAAGTTTCGCC‑GCAAGGGGGCGGGGCGGGGGG‑GTTTGTCATTCCGCAaaagag                                                                                              >  1:1683102/1‑151 (MQ=255)                                                         gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGGAAGAATGGCTGGAAGGGGCGGGGGGGGGGGGG‑GTTGGTCTTTCGAAAagagggg                                                                                             >  1:618089/1‑148 (MQ=255)                                                         gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGAAGGGCT‑GGAAGGGGAGGGGGGGGGGGG‑GGTTGTCATTTCGGAAAAGAGCg                                                                                            >  2:565503/1‑151 (MQ=255)                                                         gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATTGGCT‑GAAAGGGGCGGGGGGGGGGGG‑GGTTGTCATTTCGAAagagaggg                                                                                            >  1:200141/1‑149 (MQ=255)                                                         gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAGAAGCGCT‑GGAAGGGGCGGGGGCGGGGGG‑GGTTGTTATTTCGCAAAAGAGCg                                                                                            >  1:1457550/1‑151 (MQ=255)                                                          tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCT‑GAAAAGGGAGGGGGGGGGGGG‑GGTTGTCATTTCGAAAGAGAGGGa                                                                                           >  1:715735/1‑151 (MQ=255)                                                          tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGTTGGGGGGAAAGATGGGCT‑GAAAGGGGCGGGGGGGGGGGG‑GGTTTTCATTCCGGAagaggggga                                                                                           >  2:848244/1‑146 (MQ=255)                                                          tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAGATGCGCT‑GGAAGGGGCGGGGGCGGTGGG‑GGTTGTCATTTCCAAAGAGAGCGa                                                                                           >  1:134709/1‑151 (MQ=255)                                                           aaTTAGCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTGCAGCCAGCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGAc                                                                                          <  1:1672301/151‑1 (MQ=255)                                                           aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGAc                                                                                          <  2:1135625/151‑1 (MQ=255)                                                           aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCC‑GTAAGGGGAGGGGGCGGGGGG‑GGTTGTCATTCCGCAAGAGAGCGa                                                                                           >  1:1605730/1‑150 (MQ=255)                                                           aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAGAATGGCT‑GGGAGGGCAGGGGGGGGGGGG‑GGTTGTGAttttggcaagaaaggca                                                                                          >  1:1159140/1‑136 (MQ=255)                                                           aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGC‑GGTTGGGGGGGAAGATGGCCT‑GAAAGGGGCGGGGGTGGGGGC‑GTTTGGCATTTCGGAAGAGAGCGaa                                                                                          >  2:1218620/1‑150 (MQ=255)                                                              tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAAATGCGCT‑GAAAGGGCAGGGGGGGGGGGG‑GGTTTTCATTTCGAAAGAGAGGGACAgg                                                                                       >  2:89963/1‑150 (MQ=255)                                                                aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGTAAAATGCCGT‑GGAACGGGAGGGGGCGGGGGCGGTTTTTCTTTCCCaaaataggggaaggc                                                                                       >  2:266841/1‑138 (MQ=255)                                                                    tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                                                                                 <  1:1227366/151‑1 (MQ=255)                                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                <  1:976815/150‑1 (MQ=255)                                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                <  2:828186/150‑1 (MQ=255)                                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                <  1:1610675/150‑1 (MQ=255)                                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                <  1:115855/150‑1 (MQ=255)                                                                      ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCCCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                <  1:1545774/150‑1 (MQ=255)                                                                       cGGGCATCGGCTGGGGACGCGCGAGGTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                                                                              <  2:39301/151‑1 (MQ=255)                                                                       cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                                                                              <  2:618357/151‑1 (MQ=255)                                                                             tCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGGGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAt                                                                        <  2:465179/151‑1 (MQ=255)                                                                               ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAACGTGAtgt                                                                      >  2:1258128/1‑151 (MQ=255)                                                                               ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCG‑GGAAGGGGAGGGGGGGGGGGC‑GGTTGTCATTCCGAAAGAGAGGGACAGTCTGGGAGATCGTGgtgt                                                                      >  1:591941/1‑151 (MQ=255)                                                                                              agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGAAGCGCT‑GGAAGGGGCGGTGGGGGGGGC‑GTTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGCGGACTCGCCaga                                                       >  1:1699753/1‑151 (MQ=255)                                                                                                aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                      <  2:941442/150‑1 (MQ=255)                                                                                                aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga                                                     <  1:344851/151‑1 (MQ=255)                                                                                                   gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGG‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACCCGCCAGAGAAgg                                                  >  2:984852/1‑151 (MQ=255)                                                                                                   gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGGGCT‑GAAAGGGGAGGGGGGGGGGGG‑GGTTGTTATTCCGGAAGAGAGGGACAGGCTGGAAGAACGGGATGTGGGGCACTCGCAAGaggagg                                                  >  2:641387/1‑151 (MQ=255)                                                                                                        gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGAAGCGCT‑GAAAGGGCAGGGGGGGGGGGG‑GGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGGGCGCTCGCCAGAGAAGGCGttt                                             >  2:1153237/1‑151 (MQ=255)                                                                                                        gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCT‑GAAAGGGGAGGTGGCGGTGGG‑GGTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGGGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                             >  1:447128/1‑151 (MQ=255)                                                                                                          gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGTTGGGGGGAAAGATTCGCT‑GGAAGGGGAGGGGGCGGGGGG‑GGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGGGCACTCCCCAGAGAAGGCGATTAt                                           >  1:846120/1‑151 (MQ=255)                                                                                                          gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGAAAGAAGCGCT‑GAAAGGGAAGGGGGCGGGGGC‑GGTTGTCATTCCGAAAGAGAGGGACAGTCTGGAAGAACGGGGTGGGGCGCACTCGCAAGAGAAGGGGATTAt                                           >  2:1257301/1‑151 (MQ=255)                                                                                                           tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTAt                                           <  1:947273/150‑1 (MQ=255)                                                                                                            aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGTGGAAAAAGGGCCT‑GAAA‑GGCCGGGGGGGGGGGG‑GGTTGGCATTTCCAAAGAGAGCGCCAGTCTGGGAGATCTCTGTGGGGCGCACTCCCAAGAGAAGGCgggtgtgg                                         >  1:877241/1‑144 (MQ=255)                                                                                                            aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGGGGGGGC‑GGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGGGCACTCGCAAGAGAAGGGGATTATgg                                         >  2:209441/1‑151 (MQ=255)                                                                                                            aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGA‑GGGTGGGGGGGAAGATGCGCC‑GAAAGGGGAGGTGGGGGGGGG‑GTTTGTCATTCCGAAAGAGAGCGACAATCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGGAGGGGATTATgg                                         >  1:1316825/1‑151 (MQ=255)                                                                                                                     cATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGAAAGATGCGCT‑GGAAGGGGGGGGGGGGGGGGC‑GTTTGTCATTTCGGAAGAGAGCGACAGGTTGGAAGACCGTGATGTGGGGCACTCGCAAGAGAAGGGGAGTTTGGCGCtggtgg                                >  1:1116150/1‑151 (MQ=255)                                                                                                                       tCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGAc                              <  2:869214/151‑1 (MQ=255)                                                                                                                               ttGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAt                      <  2:1670632/151‑1 (MQ=255)                                                                                                                                tGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGGGCT‑GGAAGGGGCGGGGGGGGGGGG‑GGTTGTCCTTTCCAAAGAGAGCGGCAGGCTGGGAGATCGTGATGGTGCGCACTCGCAAGAGAAGGCGGTTATGGGGCTGGGGGACAGCCAGAtt                     >  1:106216/1‑151 (MQ=255)                                                                                                                                  ccGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGTGCTGGTGGACAGCCAGATTg                    <  2:47973/150‑1 (MQ=255)                                                                                                                                  ccGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTg                    <  1:565274/150‑1 (MQ=255)                                                                                                                                   cGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGc                  <  1:1531118/151‑1 (MQ=255)                                                                                                                                     aaGTGGCGGT‑GGGTGGGGGGAAAGATGCGCT‑GAAAGGGCAGGGGGCGGGGGG‑GTTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGGTTTTGGGGCTGGGGGGCAGCCCGATTGGCaa                >  2:1175293/1‑151 (MQ=255)                                                                                                                                        tGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAAc               <  2:608781/149‑1 (MQ=255)                                                                                                                                        tGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACtt             <  1:622687/151‑1 (MQ=255)                                                                                                                                         ggCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACttt            >  1:1610414/1‑151 (MQ=255)                                                                                                                                          gCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg           >  2:477803/1‑151 (MQ=255)                                                                                                                                          gCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg           >  2:252030/1‑151 (MQ=255)                                                                                                                                          gCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGGGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg           >  1:414156/1‑151 (MQ=255)                                                                                                                                              t‑ggtTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg       >  1:1515428/1‑151 (MQ=255)                                                                                                                                                  ttGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGccc    <  2:976772/151‑1 (MQ=255)                                                                                                                                                     gggTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg  >  2:853349/1‑150 (MQ=255)                                                                                                                                                        |                                                                                                                                                     ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCT‑GAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGG  >  NC_000913/354138‑354434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑