Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,021,908	C→A	R659L (CGC→CTC)	yccS ←	putative transporter, FUSC superfamily inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,021,908	0	C	A	100.0%	66.1 / NA	20	R659L (CGC→CTC)	yccS	putative transporter, FUSC superfamily inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/12);  total (8/12)

CAGTAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGCGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAATTTCAGGATTAGTTAACTGCTCCCGGTGAGCACCGAGGGCTGAGATATAGCTGGTAAACGT  >  NC_000913/1021796‑1022041                                                                                                                 |                                                                                                                                      cAGTAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGt                                                                                                             >  2:382357/1‑139 (MQ=255)    tAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAAc                                                                                                          >  1:121779/1‑139 (MQ=255)    tAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAAc                                                                                                          >  1:68590/1‑139 (MQ=255)           atcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGtcat                                                                                                   <  2:68590/139‑1 (MQ=255)           atcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGtcat                                                                                                   <  2:66795/139‑1 (MQ=255)                            ttgtACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCg                                                                                  <  1:382357/139‑1 (MQ=255)                                  gACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAAcgc                                                                                                        <  2:147454/111‑1 (MQ=255)                                  gACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAAcgc                                                                                                        >  1:147454/1‑111 (MQ=255)                                                      cTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAATTTCAgg                                                        >  2:260527/1‑139 (MQ=255)                                                       tGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAATTTCAGGa                                                       <  2:121779/139‑1 (MQ=255)                                                              ggTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAATTTCAGGATTAGTTa                                                <  1:438073/139‑1 (MQ=255)                                                              ggTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAATTTCAGGATTAGTTa                                                <  1:110182/139‑1 (MQ=255)                                                                     gTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAATTTCAGGATTAGTTAACTGCTc                                         <  1:260527/139‑1 (MQ=255)                                                                        gctgcATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAATTTCAGGATTAGTTAACTGCTCCCg                                      >  1:243399/1‑139 (MQ=255)                                                                                 gCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAATTTCAGGATTAGTTAACTGCTCCCGGTGAGCAcc                             >  1:147539/1‑139 (MQ=255)                                                                                  cTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTTATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAATTTCAGGATTAGTTAACTGCTCCCGGTGAGCACCg                            <  1:289192/139‑1 (MQ=255)                                                                                       tCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAATTTCAGGATTAGTTAACTGCTCCCGGTGAGCACCGAGGGc                       <  2:365607/139‑1 (MQ=255)                                                                                                     gCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCa                                                                                         >  1:323231/1‑59 (MQ=255)                                                                                                     gCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCa                                                                                         <  2:323231/59‑1 (MQ=255)                                                                                                            tGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAATTTCAGGATTAGTTAACTGCTCCCGGTGAGCACCGAGGGCTGAGATATAGCTGGTAAACGt  <  2:243399/139‑1 (MQ=255)                                                                                                                 |                                                                                                                                      CAGTAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGCGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAATTTCAGGATTAGTTAACTGCTCCCGGTGAGCACCGAGGGCTGAGATATAGCTGGTAAACGT  >  NC_000913/1021796‑1022041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑