Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	640,862	G→A	A370A (GCG→GCA)	ahpF →	alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	640,862	0	G	A	100.0%	44.0 / NA	14	A370A (GCG→GCA)	ahpF	alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/7);  total (7/7)

CAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAAAACGTCGACATTATT  >  NC_000913/640733‑640979                                                                                                                                  |                                                                                                                      cAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGAc                                                                                                              >  2:224123/1‑139 (MQ=255)                                 gCACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACgctgct                                                                              >  2:283920/1‑139 (MQ=255)                                                           cACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTaa                                                                                     >  1:63889/1‑106 (MQ=255)                                                           cACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTaa                                                                                     <  2:63889/106‑1 (MQ=255)                                                                     gCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCt                                          <  1:224123/139‑1 (MQ=255)                                                                          cTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGTGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAgg                                     <  1:283920/139‑1 (MQ=255)                                                                                    gTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAAtt                                                                                                                 >  1:326634/1‑53 (MQ=255)                                                                                    gTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAAtt                                                                                                                 <  2:326634/53‑1 (MQ=255)                                                                                       aaCGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGa                                                                                             >  1:61374/1‑70 (MQ=255)                                                                                       aaCGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGa                                                                                             <  2:61374/70‑1 (MQ=255)                                                                                       aaCGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAg                        >  1:207102/1‑139 (MQ=255)                                                                                              aGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAgg                                     >  1:58185/1‑119 (MQ=255)                                                                                              aGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAgg                                     <  2:58185/119‑1 (MQ=255)                                                                                                             cggTAACTCCGGCGTGGAAGCAGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAAAACGTCGACattatt  <  2:207102/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                      CAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCAAAGGCGTGACCTACTGCCCGCACTGCGACGGCCCGCTGTTTAAAGGTAAACGCGTAGCGGTTATCGGCGGCGGTAACTCCGGCGTGGAAGCGGCAATTGACCTGGCGGGTATCGTTGAGCACGTAACGCTGCTGGAATTTGCGCCAGAAATGAAAGCCGACCAGGTTCTGCAGGACAAACTGCGCAGCCTGAAAAACGTCGACATTATT  >  NC_000913/640733‑640979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑