Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,734,535	T→C	E167G (GAG→GGG)	yfiH ←	UPF0124 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,734,535	0	T	C	100.0%	39.8 / NA	13	E167G (GAG→GGG)	yfiH	UPF0124 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/5);  total (8/5)

AAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAA‑‑CCT‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGC  >  NC_000913/2734403‑2734662                                                                                                                                        |                                                                                                                                 aaaaTTTGCTCAACACCCACCGTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTTGATGCAAGCTCCACTTGCATTGGCGTCTAAAGCCATAAACGCCTCGC‑GAA‑‑Ccc‑cccccc                                                                                                                           <  1:202619/139‑1 (MQ=255)                              gcccTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCCCTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAAC‑CCC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc                                                                                               <  1:9632/136‑1 (MQ=255)                                                          gCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCCCC‑CCCCCCCCCCTTCCAAGGCCCGTGGCAAAATTCCCGGCCCTAACCAGCCAGAAATATTTTc                                                                    >  1:386730/1‑139 (MQ=255)                                                                            tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAAC‑CCC‑CCCCCCCCACTTCAAACGCGCGTGGACCAATTGCCGGCCCTACCCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAg                                                 >  1:438358/1‑139 (MQ=255)                                                                            tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAA‑‑CCC‑CCCCCCCCACTTCGTACGTGCGTGCACCAATTGc                                                                                               >  1:166783/1‑92 (MQ=255)                                                                            tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAA‑‑CCC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGc                                                                                               <  2:166783/92‑1 (MQ=255)                                                                             gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAAC‑CCC‑CCCCCCCCCCTTCGAACCCGCGTGGCCCAATTGCCGGCCCTAACCAGGCGAAAAAATTTTCCGGATTTCCAGCaaacaaga                                                >  1:237494/1‑134 (MQ=255)                                                                                                tGCTTTAGCGTCTACTGCCATAAACGCCCCCC‑GAACCCCC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa                              <  1:405246/139‑1 (MQ=255)                                                                                                         gTCTACTGCCCAAAACCCCCCCCGGAC‑‑CCC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                    <  2:606750/139‑1 (MQ=255)                                                                                                         gTCTACCGCCATAAACGCCTCCC‑GAAC‑CCC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                    <  1:99353/139‑1 (MQ=255)                                                                                                         ctCTACTGCCAAAAACCCCCCGC‑GAA‑‑CCCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                    <  2:237494/138‑1 (MQ=255)                                                                                                          tCTACTCCCATAAACCCCTCGC‑CAACCCCC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                    <  2:386730/139‑1 (MQ=255)                                                                                                            tACTGCCATAAACGCCTCGC‑GAAC‑CCC‑CCCCCCCCACTTCGAACGCGCGTGGACCATTTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATAATCAGCAAAAAAGGAAACCGTCTCTTCCAGCACCCCTGCGCACAg                 >  1:606750/1‑139 (MQ=255)                                                                                                                 ccATAAACGCCTCGC‑GAAC‑CCC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGACTATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGAACGCCTCCGCACAGTCCAc            >  1:141716/1‑139 (MQ=255)                                                                                                                           cTCGC‑GAAC‑CCC‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGCCTCTTCCAGCCCGCCTGCGCACAGTCCACGCcacccagc  >  2:564027/1‑139 (MQ=255)                                                                                                                           cTCGC‑GAAC‑CCC‑CCCCCCCCACCTCGAACCCCCTGGGACCAATTTCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAAAAGGAAACCGTCCCTTCCAGCACGCCTGCCCACAGCCCACCCcccccagc  >  2:381757/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                 AAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAA‑‑CCT‑CCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGC  >  NC_000913/2734403‑2734662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑