| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,594,132 | (C)8→9 | pseudogene (3856/3861 nt) | yneO ← | pseudogene, AidA homolog |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,594,124 | 1 | . | C | 96.2% | 88.1 / ‑2.8 | 26 | pseudogene (2/1461 nt) | yneO | pseudogene, AidA homolog |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (12/13); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01 | |||||||||||
AACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCC‑TTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCC > NC_000913/1593986‑1594255 | aaCGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCC‑TTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTg > 2:388607/1‑139 (MQ=255) cGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCC‑TTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTGTTAGCGACACGCAGTG‑‑cc < 2:447321/139‑1 (MQ=255) caGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCC‑TTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑‑cccccccc < 1:191696/139‑1 (MQ=255) caGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCC‑TTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑‑cccccccc < 1:378686/139‑1 (MQ=255) caGGCCATTGTCTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCC‑TTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑‑cccccccc < 1:526139/139‑1 (MQ=255) ttGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCC‑TTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCATAACGTTCATATCAcc > 1:741579/1‑139 (MQ=255) ccGTTAACGGTCAGTACATTCCCCGGTTTCCCCCC‑TTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGc < 1:766031/139‑1 (MQ=255) gTTAACGGTCAGTACATTCCCCGGTTTCCCCCC‑TTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAg > 1:431991/1‑139 (MQ=255) aCATTCCCCGGTTTCCCCCC‑TTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTcc < 2:316626/139‑1 (MQ=255) tttCCCCCCTTTCCCTTTTCATTTGACCCGGCCCCCCATTCCCAAGGTGGCCCCGGAAATGGTTTTTACCGACACGCGGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATcaca < 1:773683/139‑1 (MQ=255) tttCCCCCC‑TTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCCGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCacac < 1:388607/139‑1 (MQ=255) tttCCCCCC‑TTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCacac < 2:431991/139‑1 (MQ=255) tCCCCCC‑TTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCg < 2:468229/139‑1 (MQ=255) tCCCCCC‑TTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCCCCGCTAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCg < 2:127807/139‑1 (MQ=255) ttCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTAt > 1:442400/1‑139 (MQ=255) cATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTaa > 1:619158/1‑139 (MQ=255) ttGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAAtgt > 1:600824/1‑139 (MQ=255) aaCCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACAt > 1:240988/1‑110 (MQ=255) aaCCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACAt < 2:240988/110‑1 (MQ=255) aaCCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGtt > 1:178897/1‑139 (MQ=255) aaCCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGtt > 2:234033/1‑139 (MQ=255) cGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGAc < 2:280192/139‑1 (MQ=255) tGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGa < 1:186540/136‑1 (MQ=255) tGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGa > 2:186540/1‑136 (MQ=255) cgccATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGcc < 2:600824/139‑1 (MQ=255) ggTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGtt < 2:247381/103‑1 (MQ=255) ggTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTGCCCCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGtt > 1:247381/1‑103 (MQ=255) ccgccgATAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCAt > 1:557799/1‑46 (MQ=255) ccgccgATAGTGGTTTTAGCGACACGCAGTGC‑CCCCCCCCAGGCAt < 2:557799/46‑1 (MQ=255) aGCGACACGCAGTGC‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAAttt > 2:452446/1‑139 (MQ=255) cacGCAGTG‑‑‑CCCCCCCAGGCATAACGCTCATATCACCGGCAGTAGTGACACTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTTACTTGCCATATTAATGGCAGAGTCGCAACCGAAAGCAATTTtcccccc > 1:539196/1‑139 (MQ=255) | AACGTCATCAGGCCATTGTTTCCGGTATAGTTGCCGTTAACGGTCAGTACATTCCCCGGTTTCCCCCC‑TTCGCTATTCATTTGAACCGTGCCGCCATTCTCCAGGTTGCCGCCGATAGTGGTTTTAGCGACACGCAGTG‑‑CCCCCCCCAGGCATAACGTTCATATCACCTGCAGTGGTGACATTTCCCGACATCACACCGCTGTTATGGATATTAATGTGTTGACTTGCCATATTAATGGCAGAGTCGGAACCGAAAGCAATTTCCCCCCC > NC_000913/1593986‑1594255 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |