| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,763,869 | (T)7→8 | coding (1285/1617 nt) | yfjL ← | CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,763,862 | 1 | . | T | 98.3% | 199.0 / ‑3.6 | 58 | coding (1292/1617 nt) | yfjL | CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (27/30); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.38e-01 | |||||||||||
TAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGG > NC_000913/2763737‑2763995 | tAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTgcgcg < 1:644492/139‑1 (MQ=255) agagTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTgcgcgc < 2:822082/139‑1 (MQ=255) agagTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTgcgcgc > 2:237551/1‑139 (MQ=255) agagTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTgcgcgc < 1:591725/139‑1 (MQ=255) agagTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTgcgcgc > 2:253169/1‑139 (MQ=255) agTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCgg > 1:907199/1‑139 (MQ=255) agTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCgg < 1:253169/139‑1 (MQ=255) tCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTa > 1:409464/1‑139 (MQ=255) acaAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATg < 1:237551/139‑1 (MQ=255) aaCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTgcgcg < 1:1129241/113‑1 (MQ=255) aaCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTgcgcg > 2:1129241/1‑113 (MQ=255) aaCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTcc < 2:907199/139‑1 (MQ=255) aaaCGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTAc > 1:296539/1‑139 (MQ=255) aTCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTg > 2:683724/1‑139 (MQ=255) cGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGAt > 2:101527/1‑139 (MQ=255) aGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTAt > 1:952968/1‑139 (MQ=255) cATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTAtctc > 1:972426/1‑139 (MQ=255) aaaGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACt < 2:895648/122‑1 (MQ=255) aaaGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACt > 1:895648/1‑122 (MQ=255) aaaGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGc > 2:649061/1‑139 (MQ=255) aaGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCa > 1:1134106/1‑139 (MQ=255) ttGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCt > 1:66635/1‑139 (MQ=255) gCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATc > 1:429524/1‑135 (MQ=255) gCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATc < 2:429524/135‑1 (MQ=255) tGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACgg < 2:66635/139‑1 (MQ=255) gCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGc < 2:409464/139‑1 (MQ=255) gCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGc < 1:149359/139‑1 (MQ=255) ccATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 2:301919/139‑1 (MQ=255) ccATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 2:1134106/139‑1 (MQ=255) ccATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 2:822979/139‑1 (MQ=255) ccATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 1:101527/139‑1 (MQ=255) aaTTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAat < 1:1170174/139‑1 (MQ=255) cGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTcc > 2:1007707/1‑87 (MQ=255) cGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTcc < 1:1007707/87‑1 (MQ=255) cGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAAt > 2:472276/1‑128 (MQ=255) cGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAAt < 1:472276/128‑1 (MQ=255) tGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGc < 2:480146/115‑1 (MQ=255) tGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGc > 1:480146/1‑115 (MQ=255) tGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTgcgcg < 2:748465/50‑1 (MQ=255) tGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTgcgcg > 1:748465/1‑50 (MQ=255) tGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCg > 2:603893/1‑139 (MQ=255) gCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCt < 1:649061/139‑1 (MQ=255) aaGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTAc > 2:354588/1‑77 (MQ=255) aaGCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTAc < 1:354588/77‑1 (MQ=255) gCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGc < 2:952968/139‑1 (MQ=255) gCAGGTAGTTATCGTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGc < 2:296539/139‑1 (MQ=255) gTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATc < 2:987965/131‑1 (MQ=255) gTCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATc > 1:987965/1‑131 (MQ=255) tCTTTAATGTCGAGGTT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTcc < 2:799385/55‑1 (MQ=255) tCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTcc > 1:799385/1‑55 (MQ=255) tCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTATAACTGATCCCGTATCTCCGGCAATCTGACGGCa > 2:413974/1‑94 (MQ=255) tCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTATAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 1:413974/94‑1 (MQ=255) tCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACaa < 1:919203/63‑1 (MQ=255) tCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACaa > 2:919203/1‑63 (MQ=255) tCTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGa > 1:70091/1‑139 (MQ=255) cTTTAATGTCGAGGAT‑TTTTTTTGTGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGAt > 1:417654/1‑139 (MQ=255) cTTTAATGTCGAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGAt < 2:282844/139‑1 (MQ=255) gAGGAT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTgg > 1:436658/1‑139 (MQ=255) | TAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGCTGATCACCTTCTGG > NC_000913/2763737‑2763995 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |