Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,417,546	(T)8→7	coding (218/276 nt)	racC ←	Rac prophage, uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,417,539	0	T	.	100.0%	88.1 / NA	21	coding (225/276 nt)	racC	Rac prophage, uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (10/11);  total (10/11)

ATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTTC  >  NC_000913/1417414‑1417667                                                                                                                              |                                                                                                                                 aTTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCggg                                                                                                                    <  2:153210/139‑1 (MQ=255)                            ccGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGca                                                                                         >  1:292860/1‑139 (MQ=255)                                 tACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACAttt                                                                                    >  1:181690/1‑139 (MQ=255)                                          ttCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGacac                                                                           >  2:202399/1‑139 (MQ=255)                                                    gcgcCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAg                                                                 >  2:393016/1‑139 (MQ=255)                                                              cAGGCTATTTCTTTCAAATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACAACTTCATCAAGTGTTTTGACg                                                       <  2:348427/139‑1 (MQ=255)                                                                    aTTTCTTTCAGATTTCACCTTTTAATTTAATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGGAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCt                                                 >  2:317477/1‑139 (MQ=255)                                                                         tttcAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATgg                                            <  2:110047/139‑1 (MQ=255)                                                                             aGATTTCACCTTTTACTTTCATTGCAATTAGAGATGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTTTTGGACCa                                        >  2:345826/1‑139 (MQ=255)                                                                              gATTTCTCCTTTTAATTTCATTGAAATTAGAGTTGCCAGACATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATACGCACCAGGCACATTTTTTTGACACCATCATCAAGTGTTTTTACGTTGCCTGATGGACCAt                                       <  2:246197/139‑1 (MQ=255)                                                                                        tttAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAAc                             <  2:292860/139‑1 (MQ=255)                                                                                         ttAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAAcc                            <  2:91355/139‑1 (MQ=255)                                                                                                 aTTGCAATTAGAGTTGCCAGAAATTCGGC‑TTTTTTTCTGCGGGGAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGaa                    <  1:317477/139‑1 (MQ=255)                                                                                                              ttGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGatat                                 <  2:187352/113‑1 (MQ=255)                                                                                                              ttGCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGatat                                 >  1:187352/1‑113 (MQ=255)                                                                                                                gCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGa                                           >  1:374118/1‑101 (MQ=255)                                                                                                                gCCAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGa                                           <  2:374118/101‑1 (MQ=255)                                                                                                                 ccAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTAttt    >  2:22371/1‑139 (MQ=255)                                                                                                                 ccAGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTAttt    >  2:6847/1‑139 (MQ=255)                                                                                                                   aGAAATTCGGC‑TTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTTc  <  2:181690/139‑1 (MQ=255)                                                                                                                   aGAAATTCGGC‑TTTCTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTTc  <  1:468910/139‑1 (MQ=255)                                                                                                                              |                                                                                                                                 ATTCGTTTTGTTCAGTGAATGATCTTGCCGGATACACACTGTTCATAGCCTGCGCCATACGCAGGCTATTTCTTTCAGATTTCACCTTTTAATTTCATTGCAATTAGAGTTGCCAGAAATTCGGCTTTTTTTTCTGCGGGCAGATTCTTTCCGATATGCACCAGGCACATTTTTTTGACACCTTCATCAAGTGTTTTTACGTTGCCTGATGGACCATCGATATCAACCACAGTGAATGGGGTTTCTTTATTTTC  >  NC_000913/1417414‑1417667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑