| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,258,242 | (A)8→9 | intergenic (‑232/+43) | yhaO ← / ← tdcG | putative transporter/L‑serine dehydratase 3, anaerobic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,258,234 | 1 | . | A | 94.1% | 53.4 / ‑3.0 | 17 | intergenic (‑224/+51) | yhaO/tdcG | putative transporter/L‑serine dehydratase 3, anaerobic |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (9/7); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AA‑AAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATA > NC_000913/3258096‑3258365 | gTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATcac < 1:421678/139‑1 (MQ=255) aaTAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑aa‑aaaaaa < 1:331336/139‑1 (MQ=255) tAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑aa‑aaaaaaag > 1:132625/1‑137 (MQ=255) aaaTGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAA‑AAAAAAGGTGca < 1:223915/139‑1 (MQ=255) tCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAA‑AAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATg > 2:145880/1‑139 (MQ=255) gATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAA‑AAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCa < 2:89297/123‑1 (MQ=255) gATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAA‑AAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCa > 1:89297/1‑123 (MQ=255) aGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAA‑AAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGtc < 2:185097/139‑1 (MQ=255) tCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAA‑AAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCg < 2:39992/110‑1 (MQ=255) tCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAA‑AAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCg > 1:39992/1‑110 (MQ=255) gctaataAATTAATCGCAGAAAAATATCGATTTCCTATTATCGGCGGATCAC‑AATACAAAAGGTTATACTCATTTCACGCAGGGATGGATCATGCCAGCATAGTTAGCCGCAGACCCCTTTAATGGCCAGTCCTCcgct > 1:94472/2‑138 (MQ=255) taAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAA‑AAAAAAGGTGCACATTTGTGCACCCGAGGATGAAAGCTGACAACAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATg > 2:308890/1‑139 (MQ=255) aaTCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCACAAA‑AAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAACAATGTCAGCCGCAGACCACTTTAATGGCCTGTCCTCCGCGTGATGTTTcgc < 1:308890/139‑1 (MQ=255) tatTCATTCTCTATTTGCGGCAGATCACAAA‑AAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGtt > 2:46492/1‑139 (MQ=255) tATTTGCGGCAGATCACAAA‑AAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGc > 2:256216/1‑139 (MQ=255) tttGCGGCAGATCACAAA‑AAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCg < 1:145880/139‑1 (MQ=255) gCGGCAGATCACAAA‑AAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGtt > 2:295732/1‑139 (MQ=255) gCAGATCACAAA‑AAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGa > 1:374501/1‑99 (MQ=255) gCAGATCACAAA‑AAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGa < 2:374501/99‑1 (MQ=255) aGATCACAAA‑AAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATa > 1:355819/1‑139 (MQ=255) | GTTATTAGAATAAAGAAATGATACGCGCGAGGTCAGAGAAAATCTTCACAATCAAGACTTTGAAATAACGGAGATGGATAAGAATTTTCTACTAAATTAATCGCAGGAGAATATTCATTCTCTATTTGCGGCAGATCAC‑AA‑AAAAAAGGTGCACATTTGTGCACCCAAGGATGAAAGCTGACAGCAATGTCAGCCGCAGACCACTTTAATGGCCAGTCCTCCGCGTGATGTTTCGCGGTATTTATCGTTCATATCTTTGCCGGTTTCATA > NC_000913/3258096‑3258365 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |