Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,968,260	(T)6→5	coding (175/2247 nt)	ptsP ←	PEP‑protein phosphotransferase enzyme I, GAF domain containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,968,255	0	T	.	100.0%	255.7 / NA	61	coding (180/2247 nt)	ptsP	PEP‑protein phosphotransferase enzyme I, GAF domain containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (27/34);  total (27/34)

TTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGCGTG  >  NC_000913/2968127‑2968388                                                                                                                                 |                                                                                                                                      tttACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGccc                                                                                                                            <  1:671291/139‑1 (MQ=255)  ttACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGcccc                                                                                                                           >  1:334475/1‑139 (MQ=255)   tACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGccccc                                                                                                                          >  2:121949/1‑139 (MQ=255)     cGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCgg                                                                                                                        >  2:147609/1‑139 (MQ=255)     cGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCgg                                                                                                                        >  1:430519/1‑139 (MQ=255)      ggAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCggg                                                                                                                       <  1:531566/139‑1 (MQ=255)       gAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGt                                                                                                                      <  1:1115238/139‑1 (MQ=255)       gAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGt                                                                                                                      <  1:326187/139‑1 (MQ=255)         ggggATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCg                                                                                                                    <  2:882964/139‑1 (MQ=255)          gggATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGc                                                                                                                   >  1:559609/1‑139 (MQ=255)          gggATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGc                                                                                                                   >  1:438790/1‑139 (MQ=255)            gATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGc                                                                                                                   <  2:911402/137‑1 (MQ=255)            gATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGc                                                                                                                   >  1:911402/1‑137 (MQ=255)             aTGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCAt                                                                                                                <  2:1090018/139‑1 (MQ=255)             aTGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCAt                                                                                                                <  1:250995/139‑1 (MQ=255)             aTGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCAt                                                                                                                <  1:1016605/139‑1 (MQ=255)               gTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCa                                                                                                              <  2:430352/139‑1 (MQ=255)                 aTTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAgg                                                                                                            <  1:59595/139‑1 (MQ=255)                  tttGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGgt                                                                                                           <  1:214303/139‑1 (MQ=255)                     gAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGgtagt                                                                                                        <  1:663416/139‑1 (MQ=255)                       aGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTTCGACCGCGTGG‑TTTTTCAGCCCCCGGGACGCCATCAGGTAGTaa                                                                                                      >  2:638263/1‑139 (MQ=255)                       aGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTaa                                                                                                      >  2:152556/1‑139 (MQ=255)                          tGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGt                                                                                                                      >  2:142520/1‑120 (MQ=255)                          tGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGt                                                                                                                      <  1:142520/120‑1 (MQ=255)                           ggggTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTaacaac                                                                                                  >  1:139379/1‑139 (MQ=255)                            gggTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACg                                                                                                 >  1:582045/1‑139 (MQ=255)                                           tCTGCAAGGTTTAACGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCAt                                                                                                                <  2:731408/109‑1 (MQ=255)                                           tCTGCAAGATTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCAt                                                                                                                >  1:731408/1‑109 (MQ=255)                                                aaGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGt                                                                             <  1:147609/139‑1 (MQ=255)                                                    tttATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCg                                                                                                                    >  2:195471/1‑96 (MQ=255)                                                    tttATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCg                                                                                                                    <  1:195471/96‑1 (MQ=255)                                                      tATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCg                                                                       >  2:164516/1‑139 (MQ=255)                                                      tATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCg                                                                       >  2:231258/1‑139 (MQ=255)                                                       aTCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGa                                                                      >  2:408623/1‑139 (MQ=255)                                                            ttCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGa                                                                 <  1:107153/139‑1 (MQ=255)                                                            ttCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGa                                                                 <  1:231258/139‑1 (MQ=255)                                                             tCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATc                                                                                                               >  2:194168/1‑92 (MQ=255)                                                             tCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATc                                                                                                               <  1:194168/92‑1 (MQ=255)                                                              ccGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACATTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGaa                                                                     >  2:813494/1‑133 (MQ=255)                                                              ccGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGAt                                                                                      <  1:463016/116‑1 (MQ=255)                                                              ccGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGAt                                                                                      >  2:463016/1‑116 (MQ=255)                                                              ccGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGaa                                                                     <  1:813494/133‑1 (MQ=255)                                                                      cTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATc                                                       <  2:151953/139‑1 (MQ=255)                                                                      cTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATc                                                       <  2:334475/139‑1 (MQ=255)                                                                         ccAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCAt                                                    <  2:559609/139‑1 (MQ=255)                                                                               aGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAg                                              <  1:121949/139‑1 (MQ=255)                                                                                         tCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCg                                    <  1:164516/139‑1 (MQ=255)                                                                                          cccTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCgg                                   <  2:621496/139‑1 (MQ=255)                                                                                           ccTTCATCAAACGCGAGTGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGt                                  <  2:1067458/139‑1 (MQ=255)                                                                                              tcatcaAACGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAAc                               <  1:408623/139‑1 (MQ=255)                                                                                                   aaaCGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGaa                          <  2:430519/139‑1 (MQ=255)                                                                                                     aCGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAAc                               <  1:216261/132‑1 (MQ=255)                                                                                                     aCGCGAGCGTTACAGTGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAAc                               >  2:216261/1‑132 (MQ=255)                                                                                                                    tGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCt              >  2:651499/1‑134 (MQ=255)                                                                                                                    tGCGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCt              <  1:651499/134‑1 (MQ=255)                                                                                                                      cGACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAg       >  1:268357/1‑139 (MQ=255)                                                                                                                       gACCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAgg      >  2:494011/1‑139 (MQ=255)                                                                                                                        aCCGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGc     >  2:941044/1‑139 (MQ=255)                                                                                                                         ccGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGCg    >  2:605580/1‑139 (MQ=255)                                                                                                                         ccGCGTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGCg    >  1:249640/1‑139 (MQ=255)                                                                                                                           gcgTGG‑TTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGCgtg  <  1:152556/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                      TTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGCGTG  >  NC_000913/2968127‑2968388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑