Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	380,012	1	.	G	73.0%	88.8 / 31.2	37	intergenic (‑131/+57)	frmR/yaiO	regulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base . (7/3);  new base G (17/10);  total (24/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAA‑‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAC  >  NC_000913/379874‑380135                                                                                                                                             |                                                                                                                                 cTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGaaa                                                                                                                                   >  2:244315/1‑139 (MQ=255)              aCCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTAAAGTATATAAAACTGGGGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAA‑‑GGGGGGGGG‑G‑CT‑a                                                                                                                 <  2:464920/139‑1 (MQ=255)               ccTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAA‑‑ggggggggg‑ggcta                                                                                                                  <  2:34409/139‑5 (MQ=255)                   atcatcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAATGAGGAAA‑‑ggggggggg‑ggcgagtcg                                                                                                              >  2:323128/1‑131 (MQ=255)                                        ataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAA‑‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATa                                                                                      >  2:340475/1‑139 (MQ=255)                                            gggggTATTTTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGa                                                                                        <  2:227966/134‑1 (MQ=255)                                            gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGa                                                                                        >  1:227966/1‑134 (MQ=255)                                              gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAA‑‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAgcgggc                                                                                >  2:395302/1‑139 (MQ=255)                                                gTATTCTATATGTCAATGCATACCCCCCTATAGTATAAAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAgcgggcg                                                                               <  1:39905/139‑1 (MQ=255)                                                 tATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CTCAATGGGAGGGGAAGGCCGCCCC‑GGTTggcgggcg                                                                               >  2:219189/1‑139 (MQ=255)                                                           gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAA‑‑‑GGGGGGGG‑G‑CC‑CATCCGGAGGGAAAGGCGCCCCGGGAAAGCGGGGGGAAGAGGGAAta                                                                   >  2:26503/1‑138 (MQ=255)                                                             cAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑GT‑AATCGGCAGGGAAGGGCGCCCC‑GGATAGCGGGGGGGAGGAGGaagaa                                                                  >  2:426466/1‑136 (MQ=255)                                                              aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAg                                                                 >  2:385520/1‑139 (MQ=255)                                                                tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGaa                                                               >  2:70799/1‑139 (MQ=255)                                                                  cagaCCCCCCTGTAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAAtt                                                             >  1:289621/4‑139 (MQ=255)                                                                     aCCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAA‑‑GGGGGGGGG‑G‑CT‑AATCgg                                                                                                            >  2:446594/1‑89 (MQ=255)                                                                     aCCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAA‑‑GGGGGGGGG‑G‑CT‑AATCgg                                                                                                            <  1:446594/89‑1 (MQ=255)                                                                             tttatataaaACACTGGGAAAAAAAATTTTTCCCGGTAAAGTGGTCGGGAAAACAAAGGGAAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAg                                                   <  1:426466/135‑1 (MQ=255)                                                                                 tatataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACggg                                              >  1:241828/1‑139 (MQ=255)                                                                                   tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                            >  1:468168/1‑139 (MQ=255)                                                                                   tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                            >  1:79200/1‑139 (MQ=255)                                                                                     taACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                                          <  2:241828/139‑1 (MQ=255)                                                                                      aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAGGGGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCCCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                                          >  2:135734/1‑139 (MQ=255)                                                                                      aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCa                                         >  1:150763/1‑139 (MQ=255)                                                                                        ctcTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCa                                                                                                          <  1:287096/71‑1 (MQ=255)                                                                                        cacTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAGA‑‑GGGGGGGGG‑GCCT‑AATCGGAAGGGAGGGCCGCCCC‑GGATAGGGGGGGGC‑GGAGGGAGCAGGAATTTCAGGTCCAACGGGGTGCCAgg                                      >  1:2779/1‑138 (MQ=255)                                                                                        cacTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAGGGGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGTaa                                        >  2:186803/1‑139 (MQ=255)                                                                                        cacTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCa                                                                                                          >  2:287096/1‑73 (MQ=255)                                                                                           tGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg                                    >  2:269895/1‑139 (MQ=255)                                                                                           tGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg                                    >  2:62818/1‑139 (MQ=255)                                                                                             gagaATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                                  >  2:30777/1‑139 (MQ=255)                                                                                             gagaATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCGGCAAGTTGCa                                  >  2:404108/1‑139 (MQ=255)                                                                                             gagaATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAA‑‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                                          >  2:264776/1‑130 (MQ=255)                                                                                             gagaATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAA‑‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                                          <  1:264776/130‑1 (MQ=255)                                                                                                aaTAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAA‑‑GGGGGGGGGCG‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAc                               >  2:268033/1‑139 (MQ=255)                                                                                                            ccGGTGAATGTGGTC‑GGAAAACAAAGAGGAAA‑‑GGGGGGGGA‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCATAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatcatc                  >  1:232295/1‑139 (MQ=255)                                                                                                                   aTGTGGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTgg            <  1:385520/139‑1 (MQ=255)                                                                                                                     gtggtg‑GGAAAGAGAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTgggg          <  1:404108/139‑1 (MQ=255)                                                                                                                     gtgGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTgggg          <  2:468168/139‑1 (MQ=255)                                                                                                                     gtgGTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTgggg          <  1:30777/139‑1 (MQ=255)                                                                                                                       ggTC‑GGAAAACAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgtg        <  2:204549/139‑1 (MQ=255)                                                                                                                              aaaaCAAAGAGGAAAG‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAc  <  1:70799/139‑1 (MQ=255)                                                                                                                                             |                                                                                                                                 CTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTC‑GGAAAACAAAGAGGAAA‑‑GGGGGGGGG‑G‑CT‑AATCGGCAGGGAAGGCCGCCCC‑GGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAC  >  NC_000913/379874‑380135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑