| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 380,012 | 2 | . | G | 22.4% | 101.3 / 44.7 | 49 | intergenic (‑131/+57) | frmR/yaiO | regulator protein that represses frmRAB operon/outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (24/14); new base G (7/4); total (31/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACAC > NC_000913/379902‑380140 | agacagataCTATAGGGGGGTATTATATATGTCAATGCAAACCCCCCTATAGTATAAAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAgg < 1:988114/133‑1 (MQ=255) gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGGAGGGAAGGCCGCCCCGGAGAgcggg > 2:13052/1‑139 (MQ=255) gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGAtctgtct > 2:988114/1‑133 (MQ=255) tatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGTCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg > 2:72330/1‑139 (MQ=255) gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAt > 1:622169/1‑139 (MQ=255) aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCa > 2:913201/1‑139 (MQ=255) aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAg > 1:912643/1‑139 (MQ=255) tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATGGGCGGGGAAGGCCGCCCCGGATAGGGGGGGGCAGAAGGAATAAGaa > 1:941987/1‑139 (MQ=255) tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGCGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGaa > 2:1074413/1‑139 (MQ=255) aCCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTcc > 1:661729/1‑139 (MQ=255) aGTATAAAAAAATGGAGAAAAAAATTTATCCGGGGAAAGGGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACg < 2:941987/139‑1 (MQ=255) tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGcg > 1:951687/1‑138 (MQ=255) tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt > 2:296072/1‑139 (MQ=255) tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAAACGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt > 1:420242/1‑139 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACACAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAGGGCCGCCCCGGATAGCGGGCGGGGGAAGGAATCAGAATTCCCAGGTCAGACGGGCGGCAAg > 1:464170/1‑139 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAGGGGGGGGGGGGCAAATAGGCCGGGGAGGGCGCACCGGAAAGCGGGCGGCAGAAGGGAACGAAAGTTCCAGGTCAAACgggagggca > 1:745953/1‑133 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGccc > 1:205687/1‑86 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGGAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg > 2:262478/1‑139 (MQ=255) cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg > 1:719278/1‑139 (MQ=255) cacTGGAGAATAAAATTTATCCGGGGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGccc < 2:205687/86‑1 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg < 2:919024/94‑1 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg > 1:919024/1‑94 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAgg > 1:589143/1‑77 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAgg < 2:589143/77‑1 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg > 2:860518/1‑139 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc > 2:983505/1‑139 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa > 2:1008518/1‑139 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa < 2:661729/139‑1 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa < 1:860518/139‑1 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGAAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTgaa > 1:754932/1‑137 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAg > 2:370736/1‑139 (MQ=255) gagaATAAAATTTATCCGGTGAATGGGGGGGCAACAAAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa < 1:262478/139‑1 (MQ=255) ggaAAAAAATTTTTCCGGGGAATGTGGGGGGAAAACAAAGAGGA‑AAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa < 1:185165/138‑1 (MQ=255) agaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa < 1:370736/139‑1 (MQ=255) aaTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AATGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGGTAGCGGACGGCAGAAGGGATGAGAATTTCCAGGTCAGACGGGCTGCAAGTTGAAGa > 1:819603/1‑139 (MQ=255) tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAcc > 1:681499/1‑139 (MQ=255) aTCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCGGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaaac > 2:185165/1‑138 (MQ=255) ccGGTGAATGTGGTCGGAAAACAAAGGGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGaa < 1:545354/85‑1 (MQ=255) ccGGTGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGaa > 2:545354/1‑85 (MQ=255) gggAAGGTGGTCGGAAAACAAAGAGGAGAG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCgg < 2:464170/137‑1 (MQ=255) gTGAATGTGGTCGGAAAACAAAGAGGA‑AA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatcatc > 2:49681/1‑136 (MQ=255) gTGAATGTGGTCGGAAAACAAAGAGGA‑AA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatcatc < 1:49681/136‑1 (MQ=255) tGAATGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATGGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGt > 1:27218/1‑139 (MQ=255) aaTGTGGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTg < 1:1074413/139‑1 (MQ=255) tgtgGTCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTggg < 2:622169/139‑1 (MQ=255) tCGGAAATCAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCa > 1:772784/1‑104 (MQ=255) tCGGAAAACAAAGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCa < 2:772784/104‑1 (MQ=255) gAAAACAAAGAGGA‑AAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGa < 2:556873/73‑1 (MQ=255) gAAAACAAAGAGGA‑AAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGa > 1:556873/1‑73 (MQ=255) aaaCAAAGAGGA‑AAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAc < 2:681499/139‑1 (MQ=255) aaaCAAAGAGGA‑AAGGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAc < 1:913201/139‑1 (MQ=255) aaaGAGGA‑AAG‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCacac < 2:719278/139‑1 (MQ=255) a‑aa‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc > 2:674360/1‑86 (MQ=255) a‑aa‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc < 1:674360/86‑1 (MQ=255) | TGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGA‑AA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACAC > NC_000913/379902‑380140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |