| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 671,531 | A→G | G25G (GGT→GGC) | holA ← | DNA polymerase III, delta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 671,531 | 0 | A | G | 95.0% | 140.5 / ‑1.0 | 40 | G25G (GGT→GGC) | holA | DNA polymerase III, delta subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (23/15); minor base C (1/1); total (24/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
AAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑ACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGAC > NC_000913/671412‑671665 | aaGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑GCCTAGTAAAAGATAcgccg > 2:870068/1‑139 (MQ=255) cATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAg > 2:30257/1‑139 (MQ=255) ccAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑GCCTAGTAAAAGATACGCCGCGCGCAGCCCt < 1:870068/139‑1 (MQ=255) tcagtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑GCCAAGt > 1:218499/1‑111 (MQ=255) tcagtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑GCCAAGt < 2:218499/111‑1 (MQ=255) cAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGcgcgcg < 1:727483/139‑1 (MQ=255) aaTGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑Gcc > 2:1117791/1‑93 (MQ=255) aaTGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑Gcc > 1:220238/1‑93 (MQ=255) aaTGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCgg > 1:776260/1‑139 (MQ=255) tGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACCGAGGATCGT‑TGCCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTgcgcgc < 2:1124541/136‑1 (MQ=255) tGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTCG‑CCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTgcgcgc > 1:1124541/1‑136 (MQ=255) gtggtgTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCggg < 1:646278/139‑1 (MQ=255) cGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAat > 2:631982/1‑139 (MQ=255) tgtgCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCAtt > 2:868640/1‑95 (MQ=255) tgtgCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCAtt < 1:868640/95‑1 (MQ=255) cAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGc > 2:517519/1‑139 (MQ=255) gCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCgt > 2:1169278/1‑139 (MQ=255) cTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑Gcc > 1:183596/1‑52 (MQ=255) aGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTg < 2:776260/139‑1 (MQ=255) gCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAG‑CCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCg < 2:1153448/122‑1 (MQ=255) gCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAG‑CCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCg > 1:1153448/1‑122 (MQ=255) tCCTGGCTTTCCTGCAATAACAGAGGTTCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAgg > 2:933717/1‑139 (MQ=255) cTTTCCTGCAATAACAGAGGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGc > 2:233497/1‑139 (MQ=255) ttCCTGCAATAACAGAGGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCg > 2:565181/1‑139 (MQ=255) tCCTGCAATAACAGAGGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAg < 2:550524/109‑1 (MQ=255) tCCTGCAATAACAGAGGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAg > 1:550524/1‑109 (MQ=255) tGCAATAACAGAGGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGt < 1:587868/139‑1 (MQ=255) aCAGAGGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAg > 2:378791/1‑91 (MQ=255) aCAGAGGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAg < 1:378791/91‑1 (MQ=255) agGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGa > 1:582643/1‑79 (MQ=255) agGATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGa < 2:582643/79‑1 (MQ=255) ggATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAg > 2:82889/1‑59 (MQ=255) ggATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAg < 1:82889/59‑1 (MQ=255) gATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTgcgcg > 1:564249/1‑53 (MQ=255) gATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTgcgcg < 2:564249/53‑1 (MQ=255) gATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAAc > 2:450792/1‑74 (MQ=255) gATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAAc < 1:450792/74‑1 (MQ=255) gATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTc > 1:9586/1‑139 (MQ=255) gATCGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTc < 1:1169278/139‑1 (MQ=255) cGT‑T‑GCCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGAc > 2:14037/1‑139 (MQ=255) | AAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGT‑T‑ACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGAC > NC_000913/671412‑671665 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |