| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,806,042 | (C)6→7 | coding (42/759 nt) | ydiY ← | acid‑inducible putative outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,806,036 | 1 | . | C | 97.5% | 142.5 / ‑4.1 | 40 | G16G (GGC→GGG) | ydiY | acid‑inducible putative outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (19/20); minor base T (0/1); total (19/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.38e-01 | |||||||||||
GGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGA > NC_000913/1805901‑1806165 | ggagCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑‑ccc > 2:748215/1‑139 (MQ=255) tttCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGT‑CCCCCCGc < 1:897241/139‑1 (MQ=255) tgCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACa < 2:985872/126‑1 (MQ=255) tgCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACa > 1:985872/1‑126 (MQ=255) cAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGaa < 2:829663/139‑1 (MQ=255) cAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGaa > 1:938504/1‑139 (MQ=255) cAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGaa < 1:357246/139‑1 (MQ=255) ttCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGc > 1:925011/1‑139 (MQ=255) cAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCtt < 2:462291/139‑1 (MQ=255) cTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATc > 2:277420/1‑139 (MQ=255) ttCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCa < 1:1027522/139‑1 (MQ=255) cGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAag > 2:25977/1‑139 (MQ=255) gTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGaa < 1:346573/106‑1 (MQ=255) gTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGaa > 2:346573/1‑106 (MQ=255) ttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTacaa < 2:925011/139‑1 (MQ=255) gcTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACaaaa > 1:371493/1‑139 (MQ=255) cTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCa < 1:1050699/113‑1 (MQ=255) cTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCa > 2:1050699/1‑113 (MQ=255) cTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑‑cccccccg > 2:972570/1‑72 (MQ=255) cTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑‑cccccccg < 1:972570/74‑3 (MQ=255) atcCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATc > 2:336644/1‑139 (MQ=255) tcCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATcg < 2:811210/139‑1 (MQ=255) ccATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATcgc > 2:891019/1‑139 (MQ=255) aCAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTcc > 1:468789/1‑139 (MQ=255) aGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCg > 2:198241/1‑139 (MQ=255) aGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCg > 2:584455/1‑139 (MQ=255) tAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTccc > 1:455905/1‑136 (MQ=255) tAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTccc < 2:455905/136‑1 (MQ=255) tAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGa < 1:277420/139‑1 (MQ=255) atcatcGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACg < 1:576487/53‑1 (MQ=255) atcatcGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACg > 2:576487/1‑53 (MQ=255) atcatcGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTa < 2:13/139‑1 (MQ=255) catcGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAg < 1:198241/139‑1 (MQ=255) tcGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCa < 1:1016762/84‑1 (MQ=255) tcGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCa > 2:1016762/1‑84 (MQ=255) cGGCAGCTGCATTCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGAt > 1:1094583/1‑139 (MQ=255) ttCAGTGACGCAAACATGCCCCCCCCGCCAACATAACGATGGCTGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATTGCAGTCCCGGAAACTTTCTTAGGGATCACAGATTAg < 1:721343/139‑1 (MQ=255) tCAGTGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCg < 2:468789/139‑1 (MQ=255) tGACGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTccc > 2:112009/1‑139 (MQ=255) cGCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTgg < 1:336644/139‑1 (MQ=255) gCAAACATGC‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGa < 1:891019/139‑1 (MQ=255) cAAACATGC‑CCCCCCGCCAACATAACGATGACAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGa > 1:108334/1‑117 (MQ=255) cAAACATGC‑CCCCCCGCCAACATAACGATGACAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGa < 2:108334/117‑1 (MQ=255) | GGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATG‑‑CCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGA > NC_000913/1805901‑1806165 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |